Chimeric rabies glycoprotein with a transmembrane domain and cytoplasmic tail from Newcastle disease virus fusion protein incorporates into the Newcastle disease virion at reduced levels

Gui Mei Yu,Shu Long Zu,Wei Wei Zhou,Xi Jun Wang,Lei Shuai,Xue Lian Wang,Jin Ying Ge,Zhi Gao Bu 대한수의학회 2017 Journal of Veterinary Science Vol.18 No.-

Rabies remains an important worldwide health problem. Newcastle disease virus (NDV) was developed as a vaccine vector in animals by using a reverse genetics approach. Previously, our group generated a recombinant NDV (LaSota strain) expressing the complete rabies virus G protein (RVG), named rL-RVG. In this study, we constructed the variant rL-RVGTM, which expresses a chimeric rabies virus G protein (RVGTM) containing the ectodomain of RVG and the transmembrane domain (TM) and a cytoplasmic tail (CT) from the NDV fusion glycoprotein to study the function of RVG’s TM and CT. The RVGTM did not detectably incorporate into NDV virions, though it was abundantly expressed at the surface of infected BHK-21 cells. Both rL-RVG and rL-RVGTM induced similar levels of NDV virus-neutralizing antibody (VNA) after initial and secondary vaccination in mice, whereas rabies VNA induction by rL-RVGTM was markedly lower than that induced by rL-RVG. Though rL-RVG could spread from cell to cell like that in rabies virus, rL-RVGTM lost this ability and spread in a manner similar to the parental NDV. Our data suggest that the TM and CT of RVG are essential for its incorporation into NDV virions and for spreading of the recombinant virus from the initially infected cells to surrounding cells.

Control synthesis of magnetic Fe3O4–chitosan nanoparticles under UV irradiation in aqueous system

Lian-ying Zhang,Xin-jun Zhu,Han-wen Sun,Gui-rong Chi,Jun-xiao Xu,Yong-ling Sun 한국물리학회 2010 Current Applied Physics Vol.10 No.3

Novel magnetic Fe3O4–chitosan nanoparticles were synthesized via photochemical method in an emulsifier-free aqueous system at room temperature for the first time. The scanning electron microscopy (SEM)and transmission electron microscopy (TEM) results showed that the Fe3O4–chitosan nanoparticles were in regular shape with a mean diameter of 41 nm, whereas the average size in aqueous solution measured by photocorrelation spectroscopy (PCS) was 64 nm, which indicated that the nanoparticles had waterswelling properties. X-ray diffraction (XRD) patterns indicated that the Fe3O4 nanoparticles were pure Fe3O4 with a spinel structure, and the irradiation under UV light did not result in a phase change. The Fe3O4–chitosan nanoparticles were also characterized by Fourier transform infrared (FTIR) spectra, thermogravimetric analysis (TGA) and vibrating sample magnetometer (VSM). Magnetic measurement revealed that the saturated magnetization (Ms) of the Fe3O4–chitosan nanoparticles reached 48.6 emu/g and the nanoparticles showed the characteristics of superparamagnetism. The stability test showed these novel nanoparticles had high magnetic stability. The PCS and TGA results indicated that the size and chitosan content of Fe3O4–chitosan nanoparticles formed was pH- and chitosan/Fe3O4 ratio-dependent,which could be used to synthesize magnetic Fe3O4–chitosan nanoparticles with different size to meet the requirements of different applications.

Anisotropic NdFeCoZrBGa Magnet Powder with High properties Produced by Modified HDDR Process

FU Meng,LIAN Fa-zeng,WANG Ji-jie,PEI Wen-li,ZHOU Gui-qin,YANG Hong-cai 한국자기학회 2003 한국자기학회 학술연구발표회 논문개요집 Vol.13 No.2

Influences of Solution pH and Redox Potential on the Bioleaching of $LiCoO_2$ from Spent Lithium-ion Batteries

Li, Lei,Zeng, Gui-Sheng,Luo, Sheng-Lian,Deng, Xiao-Rong,Xie, Qing-Ji 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.2

The influences of solution pH and redox potential on bioleaching of $LiCoO_2$ from spent lithium-ion batteries using Acidithiobacillus ferrooxidans were investigated. Bioleaching at different initial pH and ferrous ion ($Fe^{2+}$) concentrations were carried out, and electrochemical behavior of $LiCoO_2$ dissolution was examined to study the effect of solution redox potential on the bioleaching process. The results showed maximum cobalt dissolution at initial pH of 1.5 and initial $Fe^{2+}$ concentration of 35 g/L, and cobalt dissolution showed only slight relationship with pH of solution. Nonetheless, there was improvement of cobalt dissolution at higher redox potential. The cyclic voltammograms showed that dissolution rates increase when the solution potentials are higher than 0.4 V, and rapid decrease at 1.3 V. The anodic polarization curves indicated that the corrosion, primary passive, and passivation potentials were 0.420, 0.776 and 0.802 V, respectively.

Transcriptome sequencing and analysis of sweet osmanthus (Osmanthus fragrans Lour.)

Hong Na Mu,Liang Gui Wang,Huo Gen Li,Xiu Lian Yang,Tao Ze Sun,Chen Xu 한국유전학회 2014 Genes & Genomics Vol.36 No.6

Osmanthus fragrans is a woody, evergreenspecies of shrubs and small trees that is extensively plantedin sub-tropical and temperate climates as an ornamentalplant in gardens and for its health benefits. The flower colorranges from ivory to orange to pink among different varietiesand even color difference during the whole blossom inthe sweet osmanthus. Sweet osmanthus is widely cultivatedthroughout China and other countries due to its prominentfragrance, colorful flowers, and medicinal properties. However, the scanty genomic resources in the Olea familyhave greatly hindered further exploration of its geneticmechanism on these economically important traits. In thisstudy, transcriptome sequencing of O. fragrans was performedusing the Illumina HighSeqTM2000 sequencingplatform. Next generation sequencing (NGS) of the transcriptomeof O. fragrans produced 31.7G of clean bases(211,266,818 clean reads) that were assembled into256,774 transcripts and 117,595 unigenes. Of them, 197and 237 candidate genes involved in fragrance and pigmentbiosynthesis respectively were identified based on functionannotation. Meanwhile, 1 unnamed protein and 468 functionalunknown genes were also identified. Furthermore,mRNA sequencing expression profiling of O. fragranswere compared to previous genes’. In summary, thiscomprehensive transcriptome dataset allows the identificationof genes associated with several major metabolicpathways and provides a useful public information platformfor further functional genomic studiesin O. fragransLour.

Identification and tissue distribution of odorant binding protein genes in the citrus fruit fly, Bactrocera minax (Enderlein) (Diptera: Tephritidae)

Jian Chen,Fu-Lian Wang,Lian-You Gui,Guo-Hui Zhang 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.1

Bactrocera minax is a destructive citrus pest in China. Owing to the increasing demand for diminishing pesticide applications in orchards, novel and effective control strategies are urgently needed. Odorant binding proteins (OBPs) represent potential targets that can facilitate the creation of environmentally friendly alternatives to the chemical control strategies. However, very little is known concerning OBP genes in B. minax. Here, we obtained seven novel OBP genes (BminOBP1-BminOBP7) from B. minax through a transcriptome analysis. All of them belong to classic OBPs. Subsequently, a phylogenetic tree was generated to characterize the seven OBP genes. Moreover, the tissue expression profiles of these OBP genes were determined by the real-time PCR. The results showed that three OBP genes (BminOBP3, BminOBP6 and BminOBP5) were highly expressed in antennae. The BminOBP3 and BminOBP6 were expressed primarily in antennae. BminOBP3 was male antenna-biased while BminOBP6 was female antenna-biased. The BminOBP5 showed high expression not only in antennae but also in legs. The other four OBP genes were highly expressed in non-olfactory tissues including heads, legs, wings and abdomens. Based on these results, the possible functions of BminOBPs are discussed. The present study provides bases for functional study of these OBPs in B. minax.

Expressions of meromorphic solutions of a certain type of nonlinear complex differential equations

Jun-Fan Chen,Gui Lian 대한수학회 2020 대한수학회보 Vol.57 No.4

In this paper, the expressions of meromorphic solutions of the following nonlinear complex differential equation of the form $$f^{n}+Q_{d}(z,f)=\sum_{i=1}^{3}p_{i}(z)e^{\alpha_{i}(z)}$$ are studied by using Nevanlinna theory, where $n\geq5$ is an integer, $Q_{d}(z,f)$ is a differential polynomial in $f$ of degree $d\leq n-4$~with rational functions as its coefficients, $p_{1}(z)$, $p_{2}(z)$, $p_{3}(z)$~are non-vanishing rational functions, and $\alpha_{1}(z)$, $\alpha_{2}(z)$, $\alpha_{3}(z)$ are nonconstant polynomials such that $\alpha_{1}'(z)$, $\alpha_{2}'(z)$, $\alpha_{3}'(z)$ are distinct each other. Moreover, examples are given to illustrate the accuracy of the condition.

EXPRESSIONS OF MEROMORPHIC SOLUTIONS OF A CERTAIN TYPE OF NONLINEAR COMPLEX DIFFERENTIAL EQUATIONS

Chen, Jun-Fan,Lian, Gui Korean Mathematical Society 2020 대한수학회보 Vol.57 No.4

In this paper, the expressions of meromorphic solutions of the following nonlinear complex differential equation of the form $$f^n+Qd(z,f)=\sum\limits_{i=1}^{3}pi(z)e^{{\alpha}_i(z)}$$ are studied by using Nevanlinna theory, where n ≥ 5 is an integer, Q_d(z, f) is a differential polynomial in f of degree d ≤ n - 4 with rational functions as its coefficients, p₁(z), p₂(z), p₃(z) are non-vanishing rational functions, and α₁(z), α₂(z), α₃(z) are nonconstant polynomials such that α'₁(z), α'₂(z), α'₃(z) are distinct each other. Moreover, examples are given to illustrate the accuracy of the condition.

In Situ Localisation of Superoxide Generated in Leaves of Alocasia macrorrhiza (L.) Shott under Various Stresses

Zhi-Fang Lin,Nan Liu,Gui-Zhu Lin,Chang-Lian Peng 한국식물학회 2009 Journal of Plant Biology Vol.52 No.4

Leaf discs of Alocasia macrorrhiza were treated with various stress factors, including two photo-oxidants, methyl viologen (MV) or riboflavin (RB); three pollutants, sodium bisulphite (NaHSO3), or the heavy metals lead or cadmium; or an osmotic medium, polyethylene glycol 6000. The in situ localisation sites for O2 − generation were identified using specific dye nitro blue tetrazolium as a probe. The level of superoxide production was determined by scanning the blue-stained formazan area and was defined as the percentage of pixels from the stained portion versus the total number of pixels in the entire leaf disc area. All stress factors induced the generation of O2 − in a time- or concentration-dependent pattern. Although superoxide production also was enhanced by longer time periods in untreated discs (control), the degree to which this occurred was less than that measured in leaves treated with either MV or RB. Generation sites were primarily found in the chloroplasts of stomatal guard cells and in the plasma membrane of the epidermis and mesophyll cells, indicating that they were most responsive to stress conditions. Nevertheless, the site of O2 − generation varied among these stress factors.

Tracking the movement trajectory of newly emerged adult Chinese citrus flies with insect harmonic radar

Zhang-Zhang He,Jie Luo,Lian-You Gui,Deng-Ke Hua,Tian-Hua Du,Fu-Lian Wang,Peng Liang,Yong-Fang Shi,Xuan Yang 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.3

Previous work reveals that the adult Chinese citrus fly, Bactrocera minax (Enderlein), emerges from the ground in citrus orchards and presumably disperses to alternate food sources in neighboring shrubs and trees, where it stays and becomes reproductively mature, and then later returns to the orchard to lay eggs. We investigated the trajectories of early emerged adult Chinese citrus flies, which were tracked with portable harmonic radar in 2013, 2014, 2015, and 2016 in four different habitats in Hubei province of China that presumably provided food sources for adults. This study we investigated whether early emerged adult Chinese citrus flies migrate into forests. A total of 47 adults were tracked following release in a citrus orchard. Our results showed that a total of 33 adults released in a citrus orchard flew into the forest, and 14 adults that disappeared during the tracking process in the citrus orchard. A total of 10 adults (in 2014) that were tracked and released in the forest flew to the forest. Of the adult flies investigated, 54.5% flew directly into the forest, while the remaining 45.5% finally arrived in the forest after repeatedly adjusting their trajectories. When there were Castanea mollissima Blume (Fagaceae) in the surrounding forest, the proportion of frequency of adult landing on the plant represented 58.3% and 88.5% of landing on all species in 2014 and in 2015, respectively. We conclude that early emerged adult Chinese citrus flies in the mosaic-type citrus orchard of the hilly terrain landform dispersed into adjacent forest.