Fenofibrate Increases Radiosensitivity in Head and Neck Squamous Cell Carcinoma via Inducing G2/M Arrest and Apoptosis

Liu, Jia,Ge, Yang-Yang,Zhu, Hong-Cheng,Yang, Xi,Cai, Jing,Zhang, Chi,Lu, Jing,Zhan, Liang-Liang,Qin, Qin,Yang, Yan,Yang, Yue-Hua,Zhang, Hao,Chen, Xiao-Chen,Liu, Zhe-Ming,Ma, Jian-Xin,Cheng, Hong-Yan,S Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Radiation therapy is an important treatment for head and neck squamous cell carcinoma (HNSCC). However, how to promote radiation sensitivity in HNSCC remains a challenge. This study aimed to investigate the radiosensitizing effects of fenofibrate on HNSCC and explore the underlying mechanisms. HNSCC cell lines CNE-2 and KB were subjected to ionizing radiation (IR), in the presence or absence of fenofibrate treatment. Cell growth and survival, apoptosis and cell cycle were evaluated. In addition, CNE-2 cells were xenografted into nude mice and subjected to IR and/or fenofibrate treatment. The expression of cyclinB and CDK1 was detected by Western blotting. Our results showed that fenofibrate efficiently radiosensitized HNSCC cells and xenografts in mice, and induced apoptosis and G2/M arrest via reducing the activity of the CDK1/cyclinB1 kinase complex. These data suggest that fenofibrate could be a promising radiosensitizer for HNSCC radiotherapy.

Preparation of Thermosensitive Hydrogels by means of Tandem Physical and Chemical Crosslinking

Hong-Liang Wei,Kai Yao,Zhe Yang,Hui-Juan Chu,Jing Zhu,Cun-Cai Ma,Zi-Xuan Zhao 한국고분자학회 2011 Macromolecular Research Vol.19 No.3

The tandem physical and chemical crosslinking was used to prepare injectable hydrogels. First, the terpolymers with diene groups were synthesized by the free radical copolymerization of N-isopropylacrylamide (NIPA), N,N-dimethylacrylamide (DMA) and furfuryl methacrylate (FM). Second, dienophile-functionalized terpolymers were obtained from the terpolymers of NIPA, DMA, and 2-hydroxyethyl methacrylate (HEMA) and Nmaleoyl alanine (AMI) by a coupling reaction under N,N'-dicyclohexylcarbodiimide (DCC). When the screened diene-functionalized terpolymer and dienophile-functionalized terpolymer were dissolved separately in water and mixed, physically cross-linked hydrogels formed rapidly within 10 s at 37 ℃. Subsequently, the physically crosslinked hydrogels could change gradually into chemically cross-linked hydrogels by a Diels-Alder reaction and the mechanical strength of the hydrogels could be improved. The swelling behavior study suggests that the hydrogels possess thermosensitivity and good reproducibility at different temperatures. The strategy described, i.e. incorporating the advantages of physically crosslinked hydrogels and chemically crosslinked hydrogels, has potential applications in the preparation of injectable hydrogels.

Tissue Inhibitor of Metalloproteinase-1 Pro-motes NIH3T3 Fibroblast Proliferation by Activating p-Akt and Cell Cycle Progression

Yang Lu,Shuxin Liu,Shujia Zhang,Guangyan Cai,Hongwei Jiang,Huabin Su,Xiaofan Li,Quan Hong,Xueguang Zhang,Xiangmei Chen 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.3

Tissue inhibitor of metalloproteinase-1 (TIMP-1) plays various roles in cell growth in different cell types. However, few studies have focused on TIMP-1’s effect on fibroblast cells. In this study, we investigated the effects of TIMP-1 overexpression on NIH3T3 fibroblast proliferation and potential transduction signaling pathways involved. Overexpression of TIMP-1, by transfection of the pLenti6/ V5-DESTTIMP-1 plasmid, significantly promoted NIH3T3 proliferation as determined by the BrdU array. Neither 5 nor 15 nM GM6001 (matrix metalloproteinase system inhibitor) affected NIH3T3 proliferation, but 45 nM GM6001 inhibited proliferation. TIMP-1 overexpression activated the p-Akt pathway, but not the p-ERK or p-p38 pathway. In TIMP-1-transfected cells, cyclinD1 was upregulated and p21CIP1 and p27^(KIP1) were downregulated, which promoted cell entry into the S and G2/M phases. The PI3-K inhibitor LY294002 abolished the TIMP-1-induced effects. Overexpression of intracellular TIMP-1 stimulated NIH3T3 fibroblast proliferation in a matrix metalloproteinase (MMP)-independent manner by activating the p-Akt pathway and related cell cycle progression.

Berberine Hydrochloride Impact on Physiological Processes and Modulation of Twist Levels in Nasopharyngeal Carcinoma CNE-1 Cells

Li, Cai-Hong,Wu, Dong-Fang,Ding, Hang,Zhao, Yang,Zhou, Ke-Yuan,Xu, De-Feng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Objective: The main purpose of this work was to investigate the effect of berberine hydrochloride (BH) on the proliferation, apoptosis, migration, and invasion of CNE-1 nasopharyngeal carcinoma cells. Our results shed light on the functional components of traditional Chinese herbs for potential use in modern medicine. Methods: The CNE-1 cell line was treated with different concentrations of BH and effects on cell viability and proliferation were evaluated using the Cell Counting Kit-8 (CCK-8) assay. Anti-migratory and anti-invasive actions of BH were investigated using wound healing assays and the Millicell Hanging cell culture insert system, respectively. Expression of the epithelial-mesenchymal transition (EMT)-related gene twist (Twist) was analyzed by real-time PCR and Western blotting. Apoptosis was estimated with an annexin-V fluorescein (FITC) apoptosis detection kit, as well as with reference to levels of activated caspase-3 of CNE-1 cells before and after treatment with BH utilizing fluorescence spectroscopy. Results: BH was capable of reducing proliferation and viability of CNE-1 cells in a dose- and time-dependent manner, also demonstrating anti-migratory and anti-invasive capacities which correlated with reduction in expression of Twist. Finally, BH was able to induce significant amounts of apoptosis in CNE-1 cells, as demonstrated by an increase in the activity of caspase-3 and in annexin-V staining following treatment. Conclusion: BH extracted from rhizoma coptidis demonstrated an ability to block proliferation, induce apoptosis, and impair the migration and invasion of the CNE-1 cell line Considering these properties, our results suggest that BH could be an important compound for consideration in the treatment of nasopharyngeal carcinoma.

Effects of Co-60 radiation on the activities of three main antioxidant enzymes in Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

Pumo Cai,Jingfang Hong,Cong Wang,Yanchuan Yang,Chuandong Yi,Jiahua Chen,Qinge Ji 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1

Co-60 radiation has been broadly used for pest management because it results in male sterility and a lack ofemergence of unparasitized hosts due to oxidative damage. Insect life is significantly affected by abiotic stressfactors, including Co-60 radiation. In the present study, we determined the influence of Co-60 radiation on animportant pest in southern China, the oriental fruit fly Bactrocera dorsalis (Diptera: Tephritidae). We exposed 30-h-old B. dorsalis eggs to Co-60 radiation at doses of (0, 5, 10, 15, 20, 25, 30, 35, and 40 Gy) and determined theactivity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and peroxidase (POX). CAT andPOX activity significantly increased in B. dorsalis in the 1st larvae instar after high-intensity radiation doses(25–40 Gy) and played an important role in the antioxidant response to intensive radiation. The activity of POXin B. dorsalis at the 2nd larval instar sharply decreased after different doses of radiation, except for 10 and 40 Gy. Our results demonstrated that Co-60 radiation affected the activity of antioxidant enzymes and disturbed thephysiology of B. dorsalis, especially at early stages (the 1st, 2nd larvae instar). These three antioxidant defenseenzymes cooperatively play an important role in protecting B. dorsalis from oxidative damage.

Three new constituents from the aerial parts of Tetrastigma hemsleyanum

Wang, Cai Yi,Lee, Ji Sun,Su, Xiang Dong,Li, Hong Xu,Lee, Sang Kook,Wang, Heng-Shan,Kim, Young Ho,Yang, Seo Young ELSEVIER SCIENCE B.V., AMSTERDAM 2018 Phytochemistry letters Vol.2018 No.27

Toughening polystyrene by core–shell grafting copolymer polybutadiene-graft-polystyrene with potassium persulfate as initiator

Gui Di Cai,Guang Hui Gao,Hong Yu Yang,Li Dan Zhu,Hua Liu,Guang Feng Wu,Ming Yao Zhang,Chao Zhou,Hui Xuan Zhang 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.3

Core–shell polybutadiene-graft-polystyrene rubber particles with different ratios of polybutadiene core to polystyrene shell were synthesized by an emulsion polymerization using K2S2O8 as an initiator. Then the core–shell rubber particles were blended with PS to prepare PS/PB-g-PS. The rubber particles with a size of 0.3–0.5 mm could toughen polystyrene significantly. The mechanical properties, morphologies and deformation mechanisms of samples were extensively investigated. The experimental results showed that the dispersion of rubber particles in a ‘‘cluster’’ state leads to better impact resistances. Crazing occurred from rubber particles and extended in a bridge-like manner to neighboring rubber particles parallel to the equatorial direction.

Preparation and Characterization of Porous and Composite Nanoparticulate Films of CdS at the Air/Water Interface

Guanglei Ji,Kuang-Cai Chen,Yan-Gang Yang,Guoqing Xin,이용일,Hong-Guo Liu 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.9

CdS nano-particulate films were prepared at the air/water interface under Langmuir monolayers of arachidic acid (AA)via interfacial reaction between Cd2+ ions in the subphase and H2S molecules in the gaseous phase. The films were made up of fine CdS nanoparticles with hexagonal Wurtzite crystal structure after reaction. It was revealed that the formation of CdS nano-particulate films depends largely on the experimental conditions. When the films were ripened at room temperature or an increased temperature (60 oC) for one day, numerous holes were appeared due to the dissolution of smaller nanoparticles and the growth of bigger nanoparticles with an improved crystallinity. When the films were ripened further, CdS rodlike nanoparticles with cubic zinc blende crystal structure appeared due to the re-nucleation and growth of CdS nanoparticles at the stacking faults and defect structures of the hexagonal CdS grains. These structures were characterized by transmission electron microscopy (TEM), high-resolution TEM (HRTEM), and X-ray diffraction (XRD). These results declare that CdS semiconductor nanoparticles formed at the air/water interface change their morphologies and crystal structures during the ripening process due to dissolution and recrystallization of the particles.

Pressure-induced semiconductor-to-metal phase transition of a charge-ordered indium halide perovskite

Lin, Jia,Chen, Hong,Gao, Yang,Cai, Yao,Jin, Jianbo,Etman, Ahmed S.,Kang, Joohoon,Lei, Teng,Lin, Zhenni,Folgueras, Maria C.,Quan, Li Na,Kong, Qiao,Sherburne, Matthew,Asta, Mark,Sun, Junliang,Toney, Mic National Academy of Sciences 2019 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.116 No.47

<P><B>Significance</B></P><P>Metal halide perovskites attract great interest for a wide range of applications due to their remarkable optoelectronic properties. The development of environmentally friendly halide perovskite materials with various crystal structures and compositions offers unprecedented opportunities to achieve desired properties and applications. In this work, we demonstrated an In-based, charge-ordered all-inorganic halide double perovskite with the composition of Cs<SUB>2</SUB>In(I)In(III)Cl<SUB>6</SUB> synthesized by solid-state reaction. High-pressure optical properties were studied, and a pressure-driven, fully reversible semiconductor–metal phase transition was discovered. This In-based charge-ordered structure may inspire new understanding of halide perovskite as well as provide a platform for future discovery of exotic electronic phenomena such as high-<I>T</I><SUB>C</SUB> superconductivity in halide perovskite compounds.</P><P>Phase transitions in halide perovskites triggered by external stimuli generate significantly different material properties, providing a great opportunity for broad applications. Here, we demonstrate an In-based, charge-ordered (In<SUP>+</SUP>/In<SUP>3+</SUP>) inorganic halide perovskite with the composition of Cs<SUB>2</SUB>In(I)In(III)Cl<SUB>6</SUB> in which a pressure-driven semiconductor-to-metal phase transition exists. The single crystals, synthesized via a solid-state reaction method, crystallize in a distorted perovskite structure with space group <I>I</I>4/<I>m</I> with <I>a</I> = 17.2604(12) Å, <I>c</I> = 11.0113(16) Å if both the strong reflections and superstructures are considered. The supercell was further confirmed by rotation electron diffraction measurement. The pressure-induced semiconductor-to-metal phase transition was demonstrated by high-pressure Raman and absorbance spectroscopies and was consistent with theoretical modeling. This type of charge-ordered inorganic halide perovskite with a pressure-induced semiconductor-to-metal phase transition may inspire a range of potential applications.</P>

Preparation and Characterization of Porous and Composite Nanoparticulate Films of CdS at the Air/Water Interface

Ji, Guanglei,Chen, Kuang-Cai,Yang, Yan-Gang,Xin, Guoqing,Lee, Yong-Ill,Liu, Hong-Guo Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.9

CdS nano-particulate films were prepared at the air/water interface under Langmuir monolayers of arachidic acid (AA) via interfacial reaction between $Cd^{2+}$ ions in the subphase and $H_2S$ molecules in the gaseous phase. The films were made up of fine CdS nanoparticles with hexagonal Wurtzite crystal structure after reaction. It was revealed that the formation of CdS nano-particulate films depends largely on the experimental conditions. When the films were ripened at room temperature or an increased temperature ($60^{\circ}C$) for one day, numerous holes were appeared due to the dissolution of smaller nanoparticles and the growth of bigger nanoparticles with an improved crystallinity. When the films were ripened further, CdS rodlike nanoparticles with cubic zinc blende crystal structure appeared due to the re-nucleation and growth of CdS nanoparticles at the stacking faults and defect structures of the hexagonal CdS grains. These structures were characterized by transmission electron microscopy (TEM), high-resolution TEM (HRTEM), and X-ray diffraction (XRD). These results declare that CdS semiconductor nanoparticles formed at the air/water interface change their morphologies and crystal structures during the ripening process due to dissolution and recrystallization of the particles.