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Guang-tao Li,Deng-pan Qiao,Hong-sheng Sun,Li-hua He 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.12
In order to improve the production efficiency of mine and ensure production safety and aiming at the unsatisfying blasting effects and uneven rock fragments existing in the exploitation blasting and construction process in Manjiazhai stope of Hualian Zinc and Indium Stock company, this paper analyzes and optimizes loosening blasting parameters of Open-pit deep-hole benches based on the blasting mechanism and engineering practical experience of wide hole distance and small resistance lines. Utilizing the numerical simulation of finite element software with ANSYS/LS-DYNA various schemes, analysis validations on the blasting stress field under different parameters are conducted. The simulation results show that: When the holes and rows spacing are 15×9, 16×9, 17×8, the effective stress of discrete cells between blast holes is bigger than the dynamic tensile strength that rocks can be broken. The site experimental investigation is conducted. The blasting effect assessment shows that the control rates of the big size rocks when the holes and rows spacing are 16×9 is 1.904% lower than those when those are 15×9, and 1.079% lower than those are 17×8. And the shovel mucking efficiency and composite economic results are better than that of the other two groups of parameters and confirmed the best blasting parameter design.
He, Jiang-Bo,Zhu, Hua-Jie,Luo, Gui-Fen,Liu, Guang-Ming,Chen, Hao,Li, Yan,Chen, Shaopeng,Lu, Xin,Zhou, Guochun,Cheng, Yong-Xian Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.8
The whole plant of Euphorbia helioscopia is an important traditional Chinese medicine. Fom its BuOH soluble extract, one new lactam (1), three new terpenoids (2-4) including a new naturally occurring compound, and three known compounds were isolated. Their structures were identified by spectroscopic evidences. In particular, the absolute configurations of side chain of compounds 1 and 2 were determined using computational methods.
Jiangbo He,Hua-Jie Zhu,Gui-Fen Luo,Guang-Ming Liu,Yan Li,Hao Chen,Shaopeng Chen,Xin Lu,Guochun Zhou,Yong-Xian Cheng 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.8
The whole plant of Euphorbia helioscopia is an important traditional Chinese medicine. Fom its BuOH soluble extract,one new lactam (1), three new terpenoids (2-4) including a new naturally occurring compound, and three known compounds were isolated. Their structures were identified by spectroscopic evidences. In particular, the absolute configurations of side chain of compounds 1 and 2 were determined using computational methods.
Gene Mapping Related to Yellow Green Leaf in a Mutant Line in Rice (Oryza sativa L.)
Peng Du,Ying Hua Ling,Xian Chun Sang,Fang Ming Zhao,Rong Xie,Zheng Lin Yang,Guang Hua He 한국유전학회 2009 Genes & Genomics Vol.31 No.2
A mutant, which derived from the restorer line Jinhui10 treated with EMS, showed completely yellow green leaves, and it had low chlorophyll content and poor agronomic characteristics during the growing stage. The F1 plants from the cross between normal × the mutant showed normal green leaves, and the segregation ratio of normal to yellow green leaves was 3:1 in F2 population. It indicated that the trait was controlled by a single recessive nuclear gene, temporarily designated as ygl3. The gene ygl3 was mapped between RM468 and RM3684 with genetic distances 8.4 cM and 1.8 cM on chromosome 3. This result would be used as genetic information for fine mapping and map-based cloning of ygl3 gene.
Xiaoqing Tian,Ying-Hua Ling,Li-kui Fang,Peng Du,Xian-Chun Sang,Fang-Ming Zhao,Yun-Feng Li,Rong Xie,Guang-Hua He 한국유전학회 2013 Genes & Genomics Vol.35 No.1
Chlorophyll is an important photosynthetic pigment in the process of photosynthesis in plants and photosynthetic bacteria. Genes involved in chlorophyll biosynthesis in Arabidopsis and photosynthetic bacteria have been well documented. In rice, however, these genes have not been fully annotated. In this paper, a yellow-green leaf gene, yellow green leaf3 (ygl3) was cloned and analyzed. ygl3 encodes magnesium chelation ChlD (D) subunit,a key enzyme for chlorophyll synthesis, resulting in a yellow-green leaf phenotype in all growth stages in rice. Expression content of ygl3 is highest in the leaf blades,followed by the leaf sheaths, while there is virtually no expression of the gene in the stems and seeds. The subcellular structure and protein content of the photosynthetic system of the ygl3 mutant were revealed by transmission electron microscopy, BN-PAGE, and western blotting. The results show that the mutation of the ygl3 gene indirectly leads to a decrease in the protein content of the photosynthetic system and severely obstructs the formation of granum thylakoids.
Gui Chun Wang,Jun Ping He,Deng Feng Hong,Yan Zhou Xie,Zheng Hua Xu,Ping Wu Liu,Guang Sheng Yang 한국유전학회 2007 Genes & Genomics Vol.29 No.4
9012AB is a recessive genic male sterility (RGMS) line in rapeseed, of which the male sterility is controlled by two pairs of recessive duplicate male sterile genes (Bnms3 and Bnms4) interacting with a recessive epistatic suppressor gene (esp). The recessive homozygosity at the esp locus (espesp) can suppress the expression of the recessive male sterility trait in homozygous plants (Bnms3Bnms3Bnms4Bnms4) and result in fertility restoration. A F2 population of 188 plants, derived from self-pollinated progenies of a 9012 AB fertile plants (BnMs3Bnms3Bnms4Bnms4EspEsp), was conducted to identify molecular markers linked to the recessive male sterility gene (Bnms3). By amplified fragment length polymorphism (AFLP) assay combining with bulked segregant analysis (BSA), 13 markers linked to Bnms3 were identified. Linkage analysis indicated that 13 AFLP markers were tightly linked to the Bnms3 gene with a genetic distance varying from 1.3 cM to 7.1 cM. Among them, one marker was co-dominant marker, 6 markers were in coupling phase with Bnms3, and the others were in repulsion phase with Bnms3 gene, One AFLP marker with a genetic distance of 1.4cM was further converted into a SCAR marker successfully, which have been applied in marker-assisted selection of RGMS lines and their temporary maintainers effectively.
Gene Mapping and Expression Analysis of a Novel Mutant reproduce organs absent (roa) in Rice
Nan Wang,Xian Chun Sang,Yun Feng Li,Ying Hua Ling,Fang Ming Zhao,Zheng Lin Yang,Guang Hua He 한국유전학회 2009 Genes & Genomics Vol.31 No.5
Mutant plays an important role in function analysis in plant. A rice flower mutant reproduce organs absent (roa), showing a stable inheritance during several years of study, was identified in rice (Oryza sativa L. ssp. Indica) cultivar Jinhui10 treated with EMS and used in this study. This mutant showed following: elongated palea and pedicle; absence of inner three whorls of floral organs; multi-whorls glume like organs inside the lemma/palea; spikelet meristem like organ upon the pedicle. These phenotypes suggested that ROA is a key gene in rice spikelet development. Genetic analysis confirmed that the mutant traits were controlled by a single recessive nuclear gene. By gene mapping, ROA was restricted between two SSR markers RM221 and RM1342 on the chromosome 2. It concluded that ROA was a novel gene involving in flower development in rice. Besides, the mutation of ROA influenced the transcription level of floral homeotic genes; the expression of floral homeotic genes decreased in roa panicle compared with wild-type, and it suggested that ROA affected flower development by influencing the expression of floral homeotic genes.
nonstop glumes (nsg), a novel mutant affects spikelet development in rice
Nan Wang,Yun-Feng Li,Xian-Chun Sang,Ying-Hua Ling,Fang-Ming Zhao,Zheng-Lin Yang,Guang-Hua He 한국유전학회 2013 Genes & Genomics Vol.35 No.2
The spikelet is a unique structure of grass plants,and its development involved with complicated molecular regulation network. nsg (nonstop glumes) mutant affecting spikelet development was identified from EMS-treated Jinhui10(Oryza sativa L. ssp. indica.). Mutant plants had normal glumes (inner rudimentary glume, empty glumes and lemma/palea) and pedicel at the early flowering stage, but had longer ones at later stage. An extra glume-like organ was found in 84 %of mutant individuals. The number of stamens decreased in most mutant individuals whereas three stigmas or two carpels were found in some mutant individuals. The mutant phenotype suggests that NSG is involved in the whole rice spikelet development. NSG was mapped to a 15 kb region on the chromosome 4. According to sequence analysis, a gene encoding a protein with C2H2 domain exhibited a 13 bp insertion, causing a frame shift in genomics DNA and cDNA in nsg. This gene was identified as the candidated gene of NSG. The mutation of NSG influenced the transcription level of some floral hometic genes. The expression of OsMADS4, OsMADS16, DL and OsMADS3 decreased distinctly, and OsMADS1 increased in nsg panicle, suggests that NSG affected spikelet development through influencing the expression of floral hometic genes.
Cong, Xin,Zhang, Xue-Ming,Zhang, Yan,Wei, Tai,He, Qi-Hua,Zhang, Li-Wei,Hua, Hong,Lee, Sang-Woo,Park, Kyungpyo,Yu, Guang-Yan,Wu, Li-Ling Elsevier 2018 Biochimica et biophysica acta. Molecular basis of Vol.1864 No.10
<P><B>Abstract</B></P> <P>Sjögren's syndrome (SS) is an inflammatory autoimmune disease that causes hyposecretion in salivary glands. Endothelial tight junctions (TJs) play crucial roles in salivation and barrier function of blood vessels. However, whether the alteration of endothelial TJs were involved in pathogenesis of SS was still unknown. Here, the ultrastructure and function of endothelial TJs in submandibular glands (SMGs) were detected by transmission electron microscopy and in vivo paracellular permeability assay in different aged NOD mouse model for SS. CFSE-labeled lymphocytes were injected into tail vein to trace the infiltration, while claudin-5 expression and distribution were detected by immunofluorescence, qRT-PCR, and western blot. Results showed that the stimulated salivary flow rate was gradually decreased and lymphocytic infiltration was found as age increased in 12- and 21-week-old NOD mice, but not 7-week-old NOD mice. Blood vessels were dilated, while endothelial TJ width and paracellular tracer transport were increased in 12-week-old NOD mice. Moreover, the injected CFSE-labeled lymphocytes were observed in SMGs of 12-week-old NOD mice. Claudin-5 level was increased and relocalized from the apical portion of neighboring endothelial cells to lateral membranes and cytoplasm in 12-week-old NOD mice. Additionally, the alteration of claudin-5 expression and distribution was further confirmed in labial salivary glands and bilateral parotid glands from SS patients. In cultured human microvessel endothelial cell line (HMEC-1), IFN-γ stimulation significantly increased claudin-5 expression. Taken together, we identified that the endothelial TJ barrier was disrupted and contributed to the development of salivary hyposecretion and lymphocytic infiltration in SS.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Endothelial tight junction barrier is disrupted in hyposecretory submandibular glands from Sjögren's syndrome mouse model </LI> <LI> The disrupted salivary endothelial barrier is linked with lymphocytic infiltration in Sjögren's syndrome mouse model </LI> <LI> The redistribution of claudin-5 is responsible for disrupted endothelial barrier in salivary glands from Sjögren's syndrome </LI> </UL> </P>