오이 추출물에 존재하는 Superoxide Dismutase의 열안정성

김은애(Eun-Ae Kim),김기남(Gi-Nahm Kim),길지은(Ji-Eun Kil),이민경(Min-Kyung Lee),김석환(Suk-Hwan Kim),서정식(Chung-Sik Suh),박인식(Inshik Park) 한국식품영양과학회 1999 한국식품영양과학회지 Vol.27 No.6

오이속의 조효소액에 존재하는 Superoxide dismutase(SOD)활성의 pH 안정성은 pH 8.0에서 가장 안정하였고 pH5.0~9.0 사이의 범위에서는 비교적 안정하였다. 최적 온도는 25℃였고 열 안정성은 60℃까지는 안정하였다. 100℃에서 5분간 보관하였을 경우에는 12%만이 남아있었다. 오이에 존재하는 SOD 활성이 섭취 후에도 안정한가를 확인하기 위한 실험에서는 위속의 pH와 동일하도록 오이속의 조효소액의 pH를 2.0으로 변형시킨 후 36.7℃에서 3시간 동안 보관 후에 잔존활성이 10%였고, 장내의 환경인 pH 7.0으로 바꾸어 6시간 동안 둔 후 잔존하는 SOD의 활성은 25%로 활성이 증가되었다. 다양한 열처리 후에 잔존하는 오이의 SOD활성은 오이속은 데치기에서(끓는 물에서 2분) 25%, 껍질은 찌는 동안에(3분) 53%, 그리고 속과 껍질로 분리하지 않은 오이는 데치기에서 27%의 활성잔존률을 보였다. 4℃에서는 20일간 보관한 후에 오이속의 조효소액은 81%활성이 있었고, 30℃에서는 17%의 활성이 남아 있었다. 투석한 결과 SOD의 활성은 변화가 없었으므로 오이속에 존재하는 SOD는 적어도 분자량이 12,000 이상의 물질로 추정된다. The superoxide dismutase(SOD) in peeled pericarp of cucumber was most stable at pH 8.0 and relatively stabe between pH 5.0 and 9.0. The enzyme was stable up to 60℃ and retained 12% by heat treatment at 100℃ for 5 min. At pH 2.0, the peeled pericarp enzyme activity was decreased to 10% by incubation for 3 hrs. However, the enzyme activity was increased above 25% after incubating the enzyme at pH 7.0 for 6 hrs. Retention of SOD activity in cucumber by various heating methods was also measured. The residual SOD activities of peeled pericarp and whole cucumber was estimated to be 25% and 27% after blanching (2 min), respectively. The skin enzyme retained 53% of its activity after steaming (3 min). When the peeled peri carp enzyme was incubated at 4℃ for 20 days, the enzyme activity remained about 81%. However, when the enzyme incubated at 30℃ for 20 days, the peeled pericarp enzyme activity decreased to 17% of its original activity. The enzyme activity of peeled pericarp cucumber was not changed after exhaustive dialysis for 3 days, which indicated that the SOD activity in cucumber seems to have molecular weight above 12,000.

盛土에 의한 橋臺의 側方移動에 대한 硏究

박춘식,장정욱,유기재,최성민 國立 昌原大學校 産業技術硏究所 2002 産技硏論文集 Vol.16 No.-

This Study investigated a possibility of lateral movement in the reconstruction field of Nongso bridge at Nongso-ri, Joochon-myeon, Kimhae. The methods applied in this study were the judgement formula and the Finite Element Method(FEM). The conclusions are shown in the following. 1) The application of the FEM, as well as the judgement formula resulted in the lateral movement. 2) Bridge abutment and foundation must be constructed after completing consolidation. 3) The occurrence possibility of lateral movement should be judged by the FEM that can re-present field conditions. 4) Lateral movement will take place in the case. Hence, settlement of proper countermeasure method should be followed by the construction.

골 임파종에 대한 방사선 조사 부위에 발생한 이차성 직장암 1예

엄민식,이홍기 인제대학교 2006 仁濟醫學 Vol.27 No.-

Radiotherapy (RT) has been used for many years, as a primary or additional tool in cancer treatment, but it has also been associated with the risk of developing another cancer. Although bone and soft tissue sarcomas are the most frequent second neoplasia following RT, Rectal cancer also can be occurred after pelvic radiotherapy. In this report, we present a patient with rectal cancer, which was developed at the same site where the patient used to get the RT, due to primary bone lymphoma. A 42-years-old man was diagnosed as a malignant lymphoma on the left hip in June 1996. His clinical stage was I_(BE). He received concurrent chemoradiotherapy; Radiotherapy, which was held with the dose of 61 Gy to femoral head, acetabulum and ischial tuberosity. And Chemotherapy was CHOP(cyclophosphamide, doxorubicin, vincristine, and prednisolone). One month later, the patient was presented with hematochezia due to radiation proctitis, and the symptom was soon improved with symptomatic treatment. He received regular follow-up at outpatient basis. In April 2003, he visited our hospital for the evaluation of constipation and anal pain for 2 months. Colonoscopy showed obstructive mass lesion at 10cm apart from anal verge and the biopsy has been revealed as adenocarcinoma. The computed tomography (CT) scan of pelvis showed the circumferential wall thickening of upper rectum with blurring margin. He has received 3 cycles of neoadjuvant chemotherapy with leucovorin and 5-FU in combination and then lower anterior resection with colostomy. The pathologic stage was Ⅲb (T3N1). Additional 6 cycles of adjuvant chemotherapy were administered. He has been in remission for 9 months after operation.

외고정 기구를 이용한 요골 원위부 불안정 골절의 치료

김민기,정의섭,홍기식 예수병원 1998 예수병원학술지 Vol.19 No.-

殺蟲性 結晶蛋白質 特異的인 抗體의 生産

김기윤,이정민,유형진,백길현,권무식 성균관대학교 생명과학자원연구소 1996 生命資源科學硏究 Vol.3 No.1

Bacillus thuringiensis is a gram-positive soil bacterium characterized by its ability to produce crystalline inclusions during sporulation. These parasporal bodies consist of protoxins known as crystal proteins exhibiting highly specific insecticidal activities. The Lepidoptera-specific protoxin, Cry IAc, was purified from E.coli JM103 harboring the cry IAc gene. The gene was isolated from Bacillus thuringiensis kurstaki HD-73 and subcloned into an expression vector, pKK223-3. The recombinant DNA(pOS4201) was transformed into E.coli JM103. The Cry I Ac protoxin overexpressed in the E.coli was isolated from the total proteins by differential solubility. The protoxin were trypsinized to obtain activated toxin. The proteins was resolved on SDS gel. The molecular weights of the pro-and toxin were resistered about 130,000 and 65,000 Daltons respectively. The Cry IAc toxin was used as immunogen to produce anti-Cry IAc antiserum in a rabbit. The immunogen (200㎍/ 200㎕) was mixed with an equal volume of Freund's complete adjuvant, and the mixture was hypodermically injected to the hide back (30 spots) and paws (2 spots) of the rabbit. The immunization was performed four times every two weeks. The dosage of the immunogen was decreased by half, and incomplete adjuvant was substituted for the complete one from the second immunization. The serum was prepared from the blood as described elsewhere. Immunoreactivity of the serum was examined by dot-blotting with the aid of GAR-HRP indirect immunoassay kit. It has been found that the anti-Cry I Ac antiserum recognized nano-gram quantity of antigen under these experimental conditions. The antiserum will he used for the development of immunochemical mean(s) to screen transgenic plants transformed by the insecticidal characteristics of Cry I Ac.

정신분열병 및 정신분열형 장애에 대한 올란자핀의 효과와 안전성

안용민,강대엽,권준수,김창윤,김철응,반건호,신영민,이기철,이동우,이중서,조현상,채정호,김용식 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.4

연구목적 : 본 다기관 공동임상연구는 국내 환자를 대상으로 새로운 항정신병약물인 올라자핀의 치료 효과와 안전성을 확인하고자 시행되었다. 방 법 : 1999년 7월부터 2000년 3월까지 국내 10개 병원에 방문한 정신분열병 및 정신분열형 장애 105명을 대상으로 비대조 개방 임상연구를 시행하였다. 중등도 이상의 정신병 증상을 지니거나, 부작용으로 약물교체가 필요한 경우 또는 기분장애 증상의 치료가 필요한 환자를 대상으로 하였다. 2∼7일간의 약물 배설기간 후에 1일 10mg의 올란자핀을 투여하였고, 이후로 3∼7일 간격으로 용량을 조절하여 총 8주간 투여하였다. 치료 효과는 PANSS(Positive and Negative Syndrome Scale), BPRS(Brief Psychiatric Rating Scale), CGI(Clinical Global Impression-Severity), MADRS(Montgomery-Asberg Depression Rating Scale), HAM-A(Hamilton Rating Scale for Anxiety)로 판정하였다. 안전성 평가는 자발적인 이상반응 보고, 활력징후와 혈액 검사 및 SAS(Simpson-Angus Scale), BARS(Barnes Akathisia Rating Scale), AIMS(Abnormal Involuntary Movement Scale)척도를 이용하였다. 결 과 : 105명중에서 85.7%인 90명이 8주간의 치료를 완료하였다. 평균 최빈 용량은 일일 16.1(±4.7)mg이었고, 종료시점에서의 BPRS전체 점수가 기저 상태에 비해 40%이상 감소된 반응군은 69.5%이었다. PANSS의 양성 증후군과 일반정신병리 뿐만 아니라 음성 증후군 소척도에서도 유의한 감소를 보였으며, 이 감소들은 치료 초기부터 나타나서 8주간 지속되었다. 43.8%의 피험자가 중등도 이상의 우울증상을 같이 지니고 있었으며, 올란자핀에 의해 MADRS와 HAM-A가 유의하게 감소하였다. 치료 기간 동안에 활력징후에는 뚜렷한 변화가 없었으나 체중은 8주간 지속적으로 증가하였다. SAS와 AIMS는 감소하였으며, 정좌불능증을 제외한 나머지 추체외로 증상의 발생비율도 낮았다. 임상적인 증상이나 징후를 동반하지 않는 ALT/SGPT의 상승을 보였지만 프로락틴을 포함하는 대부분의 혈액 검사상 뚜렷한 이상 변화는 없었다. 결 론 ; 비록 본 임상연구가 비대조 개방 임상연구로서 많은 제한점을 가지고 있지만, 올란자핀이 기존 약물에 내약성을 보이거나 기분증상을 동반하는 정신병에 치료 효과가 있었다. 그리고 기존 항정신병약물 치료시에 문제가 되었던 추체외로 증상과 고프르로락틴 혈증을 포함하는 대부분의 부작용면에서 안전성을 보여 주었다. Objective : This multicenter clinical trial was carried out to investigate the efficacy and the safety of olanzpine for the treatment of Korean patients. Mothod : 105 patients with schizophrenia and schizophreniform disorder, visited at 10 mental or university hospitals, had received an open and non-comparative treatment with olanzapine for 8 weeks. Patients had psychotic or depressive symptoms with the severity above moderate degree or intolerable side effects to previous antipsychotics. After a wash-out period of 2-7 days, 10mg olanzapine was prescribed initially to all the patients, and then the dosage could be adjusted within the range of 5-20mg/day of olanzapine by 3-7 days. Results : 90(85.7%) of 105 patients completed the 8-weeks trial and the mean modal dose of olanzapine was 16.1(±4.7%)mg/day. At the end of the trial, 73 patients(69.5%) were classified as responder, which was defined as 40% or more improvement in BPRS(Brief Psychiatric Rating Scale) score comparing to baseline. There was a significant reduction in the scores of PANSS(Positive and Negative Syndrome Scale)and subscales including negative symptom scores and CGI. Also weekly analysis showed that the reductions in scores were kept on for the whole period of the trial. 43.8% of all the patients had depressive symptoms at the baseline and total scores of MADRS(Montgomery-Asberg Depression Rating Scale) and HAM-A(Hamilton Rating Scale for Anxiety) were also reduced after the trials. Vital signs revealed no clinically significant changes but continuous weight gain was observed during the treatment with olanzapine. The scores of SAS(Simpson-Angus Scale) and AIMS(Abnormal Involuntary Movement Scale) for assessing the EPS(extrapyramidal symptoms)and tardive dyskinesia respectively were significantly decreased and only a few patients reported EPS as adverse events. Although mild and clinically non-significant of ALT/SGPT was observed, most laboratory parameters including plasma prolactin level showed to significant changes during the trial. Conclusions : Although this trial had many limitations because it was a non-comparative and open study, olanzapine showed high efficacy on the positive, negative and depressive symptoms in schizophrenia and schizophreniform disorder.In addition to that, olanzapine showed a substantially favorable safety profile, such as low incidence of EPS and hyperprolactinemia.

하수 염소 소독시 소독부산물 발생 특성

백영석,송민형,정경훈,권동식,이기공 한국물환경학회 2004 한국물환경학회지 Vol.20 No.3

This study was performed to investigate the disinfection efficiency and the formation characteristics of disinfection by-products(DBPs) by chlorination in the sewage effluent. The effluent was sampled from the sewage treatment plants operated in the activated sludge process and the advanced sewage process. The type of DBPs investigated were Trihalomethanes(THMs), Dichloroacetonitrile(DCAN), Chloral hydrate(CH), Dichloroacetic acid(DCAA), Trichloroacetic acid(TCAA). Major findings are as follows. First, the optimum injection concentration for chlorination in sewage effluent were found to be in the range 0.5 ~ 1.0 ㎎ Cl₂/L. Also, It was found that the chlorine dosage in the effluent of activated sludge process was higher than in the effluent of advanced sewage process. Second, the maximum formation concentration of THMs were 12.7 μg/L. The THMs formation reaction was finished in a short time of several seconds and chloroform was mainly formed. Also, it was found that the concentration of ammonium nitrogen is higher, the concentration of THMs is lower. Third, it was found that DCAA and TCAA were mainly formed as DBPs by disinfection.

Inhibitory Effects of Triterpenoids on Interleukin-8/CINC-1 Induction in LPS-Stimulated Rat Peritoneal Macrophages

Min, Bok-Gi,Lee, Gyeong-Im,Ha, Joo-Young,Min, Kyung-Rak,Kim, Ju-Sun,Kang, Sam-Sik,Kim, Young-Soo The Korean Society of Pharmacognosy 1996 Natural Product Sciences Vol.2 No.1

The CINC-1 is a member of rat interleukin-8 with chemotactic and activating properties to neutrophils. The CINC-1 induction in LPS-stimulated rat peritoneal macrophages was analyzed using a sensitive enzyme-linked immunosorbent assay. The peritoneal macrophages contained about 3 ng/ml as a basal level, and induced to maximal 18 ng/ml of CINC-1 by stimulation with 5 ${\mu}g/ml$ of LPS. Antiinflammatory steroids of dexamethasone and triamcinolon significantly suppressed the CINC-1 induction, where as aspirin and idomethacin did not show suppression. Inhibitory effects on the CINC-1 induction by natural triterpenoids having steroidal structures were analyzed. Among the 39 kinds of triterpenoids isolated from herbal medicines, acacigenin B and nigaichigoside F1 exhibited the highest suppression on the CINC-1 induction.

Treatment for relapsed acute promyelocytic leukemia: what is the best post-remission treatment?

Gi-June Min,Byung-Sik Cho,Sung-Soo Park,Silvia Park,Young-Woo Jeon,Seung-Ah Yahng,Seung-Hawn Shin,Jae-Ho Yoon,Sung-Eun Lee,Ki-Seong Eom,Yoo-Jin Kim,Seok Lee,Chang-Ki Min,Seok-Goo Cho,Jong Wook Lee,Hee 대한혈액학회 2022 Blood Research Vol.57 No.3

Background Arsenic trioxide (ATO) is the standard treatment for relapsed acute promyelocytic leukemia (APL). However, consensus on post-remission therapies is still lacking. Methods We evaluated 52 patients who experienced relapse following initial treatment of APL between 2000 and 2019 at Catholic Hematology Hospital. Among them, 41 patients received reinduction treatment, 30 with ATO-based regimen, whereas 11 with conventional intensive chemotherapy (IC). Results The ATO reinduction group showed a significantly higher second molecular complete remission (mCR2) rate, superior neutrophil and platelet recovery, and a lower infection rate than the IC reinduction group. No significant differences were observed in survival outcomes after post-remission treatment among the ATO-based (N=19), autologous (N=12), and allogeneic (N=6) hematopoietic stem cell transplantation (HSCT) groups. In the ATO-based and autologous HSCT groups, among patients with mCR2 after ATO reinduction, nine and five patients experienced a second relapse, respectively (50.7% vs. 41.7%, P=0.878). Among these patients, seven received salvage allogeneic HSCT; six remained alive. The other seven patients received ATO without HSCT. Five died from disease progression, and two survived and have been in mCR2 since. Conclusion Post-remission treatment outcomes of patients with relapsed APL were not significantly different, regardless of the treatment option, suggesting the feasibility of ATO-based treatment without HSCT in mCR2. Allogeneic HSCT may be an effective salvage treatment modality for patients with a second relapse. Owing to a few cases of relapsed APL, multicenter prospective studies may help elucidate the efficacy of each post-remission treatment.

Biological Function of Single Chain Glycoprotein Hormone Mutants

Min Kwan-Sik,Chang Yoo-Min,Chang Sun-Hwa,Lee Hyen -Gi,Lee Yun -Gun,Chang Won - Kyong,Cheong Il-Cheong 한국동물번식학회 2001 Reproductive & Developmental Biology(Supplement) Vol.25 No.1s