PCR을 이용한 국내시장에 유통중인 유전자재조합 콩 및 가공식품의 모니터링

김묘영,김재환,김현중,박선희,우건조,김해영 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.4

본 연구에서는 PCR을 이용하여 국내시장에 유통중인 원료콩과 가공식품에 epsps 또는 pat 유전자가 삽입된 유전자재조합 콩(GMS)의 사용여부를 모니터링하였다. 이러한 GMS의 검출을 위해 3쌍의 primer set을 제작하였고, 각각의 primer들은 GMS에 삽입된 유전자와 특이적으로 반응하여 PCR산물을 생성하였다. 2001년 표시제가 시행되기 이전에 생산된 총 가공식품과 이후의 제품에 대해 각각 모니터링을 수행하였으며, 표시제 이전에 생산된 제품의 경우 대부분의 미국산 원료에서 esps가 삽입된 GMS가 검출되었으나, 표시제 이후에는 검출되지 않았다. A method using PCR was developed for the monitoring of genetically modified soybean (GMS) and GMS derived foods utilized in the market. We designed 3 pairs of specific oligonucleotide primers based on epsps and pat inserted in GMS and ferritin gene as internal standards. Template DNAs isolated from soybean and processed foods were used for multiplex PCR with 3 primer sets. PCR, used with specific primer sets for GMS detection, showed the amplified DNA fragments with GMS template DNA. In this study, GMS containing epsps was detected from soy processed foods manufactured before GM food labeling system, however, GMS containing epsps or pat was not detected from soy processed foods manufactured after GM food labeling system.

빌트인 모터를 이용한 2축 제어 미세 가공기 개발

김건희,김효식,양순철,이상용,국명호,이선규,김윤중,홍권희 한국공작기계학회 2007 한국공작기계학회 추계학술대회논문집 Vol.2007 No.-

This paper describes development of low cost precision machine that has combined the Build-in Motor with a vacuum chuck. This study mainly aims to find out a cutting condition for maintaining optimum surface condition and to examine cutting characteristics of the precision machine that is equipped by diamond bites. The cutting materials is Al alloy. Several experiments were carried out to find out the main factors that affect the surface roughness such as principal axis RPM(rotation per minute), feeding speed, and cutting depth. As a result, we obtain The optimum cutting condition of the developed precision machine.

AGV 용 서스펜션의 진동 모드에 관한연구 : 중력 가속도가 5G인 경우 A Case of Gravity Acceleration is 5G

주만식,김민주,이승수,최영철,추정근,김중완,전언찬 한국공작기계학회 2000 한국공작기계학회 추계학술대회논문집 Vol.2000 No.-

This study is to choose the most proper model for AGV throughout simulation of behavior of suspension to reduce trial and error because there is no AGV treating heavy weight at harbor loading and unloading at home. Therefore, we estimate the vibration modes of the various suspension applied to AGV, which is over 75 ton included the weight of two containers on 5G using the Matlab, one of the simulation programs

Structure and Acidity Modification of Mordenite Through Isomorphous Fe Substitution

Kim, Geon Joong,Ahn, Wha Seung 한국화학공학회 1992 NICE Vol.10 No.4

A series of Fe-modified mordenite were hydrothermally synthesized. The effects of substrate composition, aging time, reaction temperature, and reaction time upon the crystallization of the mordenite were investigated. Isomorphous substitution of Fe was confirmed by FT-IR and EPR analysis. Acid strength and acid sites distribution of each catalyst were measured by pyridine TPD, which showed that the number of strong acid sites in H-Fe-mordenite are smaller than in H-Al-mordenite. Structural characteristics and ermal stability were also examined using XRD, SEM and TG, Fe-substitution into the mordenite structure have resulted in substantial decrease in thermal stability HFe-mordenite catalysts showed high selectivity towards p-xylene in toluene alkylation and influent isomerization reaction, compared with those obtained using H-Al-mordenite because of lower concentration of strong acid sites. In addition, these H-Fe-modified mordenite showed little hexane cracking activity. Deactivation in both toluene alkylation and xylene isomerization reaction and coke formation over H-Fe-mordenite in these reactions were negligible.

Hydrothermal crystallization and secondary synthesis of vanadium containing zeolites

Kim, Geon-Joong The Korea Association of Crystal Growth 1997 韓國結晶成長學會誌 Vol.7 No.3

직접 수열합성법과 변조된 처리법을 이용하여 세공경이 큰 제올라이트의 결정구조내에 바나듐원자가 치환되도록 하였다. 제올라이트 골격에 바나듐이 도입되었는지의 여부를 기기분석을 통하여 시험하였다. X선회절분석의 결과, 바나듐이 제율라이트의 구조중에 도입되면 결정골격중의 실리콘원자 대신 치환되어 결정단위세포의 확장을 나타내었다. 또한 FTIR, UV-Vis 및 Si-MAS-NMR 스펙트럼결과는 제올라이트 골격내로의 바나듐의 도입을 강하게 됫받침하였다. 제올라이트중의 알루미늄을 산처리로 용출시키면, 격자에 빈자리를 제공하여 2차수열처리법에 의한 바나듐의 도입을 가능케 하였다. The substitution of vanadium atoms into the zeolite framework structure could be applied to the large pore zeolites by means of modified treatments as well as direct hydrothermal synthesis. The incorporation of V into the zeolite framework was demonstrated by instrumental analysis techniques. The result of X-ray diffraction analysis showed that the unit cell parameters increased after incorporation of vanadium into the zeolitic lattice, indicating that the replacement of Si by the larger V atoms could cause a slight expansion in the unit cell. In addition, the results of FTIR, Uv-Vis and Si-MAS-NMR spectra strongly support the incorporation of V into the zeolite framework. Acid leaching of aluminum in zeolites can provide a vacant position in the lattice for the insertion of vansdium by secondary hydrothermal treatment.

Preparation of Ba - Ferrite Particles Using the SuperCritical Water Crystallization Method

Kim, Geon Joong,Nam, Sung Chan,Park, Sang Do 한국공업화학회 2001 Journal of Industrial and Engineering Chemistry Vol.7 No.1

Barium ferrite particles were synthesized from Ba(NO₃)₂, Fe(NO₃)₃ and KOH mixed solutions using hydrothermal crystallization in supercritical water. The experimental apparatus for production of barium ferrite is a flow-type apparatus. Fine barium ferrite particles were produced because supercritical water causes the metal hydroxides to be rapidly dehydrated before significant growth takes place. The effects of Fe/Ba ratio and reaction time on the formation, particle size, and magnetic properties of barium ferrite were studied. When the Fe/Ba molar ratio was varied from 0.5 to 12, single-phase barium ferrite powder was only produced at Fe/Ba molar ratios of 0.5 and 2. Also, with increasing residence time from 60 to 100s, the BaO 6Fe₂O₃particle size grew smaller. Especially, uniform barium ferrite particles of size 100-200 ㎚ were obtained at 100s. In this study, therefore, single-phase barium ferrite particles can be produced continuously in a reaction time of less than 3 min.

Atomistic modelling for boron diffusion in strained silicon substrate

Kim, Young-Kyu,Yoon, Kwan-Sun,Kim, Joong-Sik,Kim, Han-Geon,Won, Taeyoung Taylor Francis 2008 Molecular simulation Vol.34 No.1

<P> We discuss the issue of boron diffusion in biaxial tensile strained {001} Si and SiGe layer with kinetic Monte Carlo (KMC) method. We created strain in silicon by artificially adding a germanium mole fraction to the silicon in order to perform a theoretical analysis. The strain energy of the charged defects was calculated from ab initio calculation whereas the diffusivity of boron was extracted from the Arrhenius formula. Hereby, the influence of the germanium content on the diffusivity of the impurity atom was estimated. Our KMC study revealed that the diffusion of the boron atoms was retarded with increasing germanium mole fraction in the strained silicon layer. Furthermore, we derived the functional dependence of the in-plane strain as well as the out-of-plane strain as a function of the germanium mole fraction, which is based on the distribution of equivalent stress along the Si/SiGe interface.</P>

Preparation of TiO2 nanoparticle from Ti-salt flocculated sludge with dye wastewater.

Kim, Jong Beom,Park, Hee Ju,Shon, Ho Kyong,Cho, Dong Lyun,Kim, Geon-Joong,Choi, Sung Woo,Kim, Jong-Ho American Scientific Publishers 2010 Journal of Nanoscience and Nanotechnology Vol.10 No.5

<P>Dye wastewater flocculation using TiCl4 and FeSO4 coagulants was studied for organic removal and precipitation efficiency. Moreover, TiCl4 was assessed in a pilot-scale flocculation process to investigate organic removal and solution pH effect. A large amount of TiO2 was produced from sludge of Ti-salt flocculation in dye wastewater. This flocculation process simultaneously reduces a large amount of sludge produced from wastewater. The TiO2 nanoparticle was characterized in terms of physical and chemical properties. Results showed that 77.6% of organic matter was removed from dye wastewater when using FeSO4 as coagulant, while TiCl4 degraded 75.9% of organic matter. On the other hand, floc precipitation efficiency was better for TiCl4 if compared with FeSO4. The optimum concentration of TiCl4 for pilot-scale flocculation was found to be equal to 2.1 g/L. DWT (Dye wastewater titania) size was 15-20 nm, mainly doped with carbon atoms and exhibited a dominant anatase structure. DWT was similar to P-25 in decomposing acetaldehyde under UV-irradiation and complete photocatalytic degradation was achieved after 140 min.</P>

Synthesis and Characterization of Co-Surfactant Templated Mesoporous Materials with Enhanced Hydrothermal Stability

Kim Geon-Joong,Kim Hyun-Seok,Ko Yoon Soo,Kwon Yong Ku The Polymer Society of Korea 2005 Macromolecular Research Vol.13 No.6

Ordered mesoporous materials with a hydrothermally-stable, protozeolitic framework were prepared by exploring the direct conversion of inorganic species based on co-surfactant templating systems. To confer hydrothermal stability on the mesoporous aterials, the organic-inorganic hybrids were heat-treated in strongly basic media. Co-surfactant templating systems of cetyltrimethylammonium bromide [$C_{16}H_{13}(CH_{3})_{3}$NBr, CTAB] with 1,3,5-trim­ethylbenzene (TMB) or a nonionic block copolymer of poly(ethylene oxide )-b-poly(propylene oxide )-b-poly(ethyl­ene oxide) ($EO_{20}PO_{70}EO_{20}$) were employed to improve the hydrothermal stability of the organic-inorganic self-assembly during the solid rearrangement process of the inorganic species. The mesoscopic ordering of the pore structure and geometry was identified by X-ray diffraction, small angle neutron scattering and electron microscopy.

Preparation of iron-doped titania from flocculated sludge with iron-titanium composite coagulant.

Kim, Jong Beom,Lee, Ki Won,Park, Se Min,Shon, Ho Kyong,Shahid, Mohammad,El Saliby, Ibrahim,Lee, Woong Eui,Kim, Geon-Joong,Kim, Jong-Ho American Scientific Publishers 2013 Journal of Nanoscience and Nanotechnology Vol.13 No.6

<P>The main drawback of flocculation process with dye wastewater is the large amount of unrecyclable sludge which needs disposal. A novel process using Ti-salt flocculation to purify wastewater was developed to produced sludge that can be calcined to produce titania. In this study, iron-doped TiO2 nanomaterial was successfully produced from sludge obtained by the flocculation of dye wastewater with a composite floculant including TiCl4 and FeSO4. The titania was characterised using scanning electron microscopy (SEM/EDX), transmission electron microscopy (TEM), X-ray diffraction (XRD), and the photodecomposition of acetaldehyde. The XDR results showed that the anatase and rutile structures were found after sludge calcination at 550 degrees C and 640 degrees C respectively. The elemental analyses were carried out using EDX. The rutile titania sample consisted of Ti (35.7 wt.%), Fe (14.7 wt.%), O (42.3 wt.%), P (2.6 wt.%) and Ca (4.7 wt.%). The photocatalytic activity was monitored for the photodecomposition of gas acetaldehyde. Iron-doped titania seems to play an important role in increasing the photocatalytic activity under UV light irradiation.</P>