An Ester Extract of Cochinchina Momordica Seeds Induces Differentiation of Melanoma B16 F1 Cells via MAPKs Signaling

Zhao, Lian-Mei,Han, Li-Na,Ren, Feng-Zhi,Chen, Shu-Hong,Liu, Li-Hua,Wang, Ming-Xia,Sang, Mei-Xiang,Shan, Bao-En Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Cochinchina momordica seeds (CMS) have been widely used due to antitumor activity by Mongolian tribes of China. However, the details of the underlying mechanisms remain unknown. In the present study, we found that an EtOAc (ethyl ester) extract of CMS (CMSEE) induced differentiation and caused growth inhibition of melanoma B16 F1 cells. CMSEE at the concentration of $5-200{\mu}g/ml$ exhibited strongest anti-proliferative effects on B16 F1 cells among other CMS fractions (water or petroleum ether). Moreover, CMSEE induced melanoma B16 F1 cell differentiation, characterized by dendrite-like outgrowth, increasing melanogenesis production, as well as enhancing tyrosinase activity. Western blot analysis showed that sustained phosphorylation of p38 MAP accompanied by decrease in ERK1/2 and JNK dephosphorylation were involved in CMSEE-induced B16 F1 cell differentiation. Notably, 6 compounds that were isolated and identified may be responsible for inducing differentiation of CMSEE. These results indicated that CMSEE contributes to the differentiation of B16 F1 cells through modulating MAPKs activity, which may throw some light on the development of potentially therapeutic strategies for melanoma treatment.

Preventative effects of Lactobacillus plantarum YS-3 on oxazolone-induced BALB/c colitis in mice

Xia Feng,Jing Zhang,Yu Qian,Ruokun Yi,Peng Sun,Jianfei Mu,Xin Zhao,Jia-Le Song 한국응용생명화학회 2018 Applied Biological Chemistry (Appl Biol Chem) Vol.61 No.3

In this study, the preventative effects of Lactobacillus plantarum YS-3 (LP-YS3) on colitis were studied using an in vitro animal experiment. Oxazolone was administered to BALB/c mice to induce colitis, and the preventive effects of LP-YS3 were determined using serum- and tissue-related indexes. The mice treated with LP-YS3 showed a significant decrease (p\0.05) in disease activity index for colitis by inhibiting colon shortening and increasing colon weight/length ratio. The application of LP-YS3 resulted in a significant reduction in myeloperoxidase, nitric oxide, and malondialdehyde activities and a decrease in glutathione activity (p\0.05) in mouse colon tissues. LP-YS3 also significantly increased serum interleukin-2 and reduced interleukin-10 cytokines levels in mice (p\0.05). Reverse transcription-polymerase chain reaction and western blot assays showed that LP-YS3 application resulted in a significant increase in neuronal nitric oxide synthase, endothelial nitric oxide synthase, c-Kit, and stem cell factor expressions and a decrease in inducible nitric oxide synthase, interleukin-8, and C-X-C motif chemokine receptor 2 expressions in mouse colonic tissues (p\0.05). These findings indicate that LP-YS3 imparts preventative effects on oxazoloneinduced colitis in mice.

Ten Year Literature on Psychological and Behavioral Interventions Against Cancer: a Terms Analysis

Feng, Rui,Chai, Jing,Wang, De-Bin,Xia, Yi,Cheng, Peng-Lai,Dai, Zhao-Yang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

We here performed a systematic review of PBIC literature using terms analysis in a hope of both identifying potential trends and patterns and exploring methods leveraging traditional literature reviews in this specific area. Articles meeting inclusion criteria were retrieved from PUBMED and translated into dichotomized article records representing presence or non-presence of MeSH terms and a metric consisting of numbers of times of co-occurrence between all pairs of terms identified using a self-designed program. The occurrence of and relations among the terms were calculated and visualized using Excel2007 and UCINET respectively. A total of 1,742 terms were identified from 997 articles retrieved. Put in a descending order, the lines representing the times of term occurrence formed a typical hyperbolic curve; when plotted along the x-axis of whole MESH terms, the lines clustered within four specific regions. Comparison of term occurrence between 2002 and 2011 revealed priority changes in population and subjects (from general groups to priority groups), intervention approaches (from medicine to exercise and psychotherapy), methodology and techniques (from cohort studies to randomized controlled trials) and outcomes (from health and mental health to quality of life, depression etc.). Networks of the terms featured a number of closely linked groups of topics including method and questionnaires, therapy and outcomes, survival management, psychological assessment and intervention, behavioral intervention (individual and community oriented). Terms analysis revealed interesting trends and patterns about PBIC publications and both the analysis methods and findings have implications for future research and literature reviews.

Parameter design method for the heat transfer performance of a fan-driven gearbox under planetary gear opening and oil-return conditions

Feng-xia Lu,Lijuan Yuan,Zhiqiang Zhao,Chun-lei Wang,He-yun Bao 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.9

On the basis of the computational fluid dynamics (CFD) method, this study calculates the surface convective heat transfer coefficients of the sun gear, planetary gear, ring gear, and bearing before and after the opening of the planetary gear of a fan-driven gearbox. A numerical fitting method is used to establish the calculation model of the convective heat transfer coefficients of the sun gear, planetary gear, ring gear, and bearing under the open-hole oilreturn condition. The convective heat transfer coefficients of each element are compared before and after the hole is bored, and the design method for the gearbox parameters with the objective of heat transfer performance is proposed. Result shows that the convective heat transfer coefficients of each element are increased after oil is returned from the hole of the planetary gear, and the change rate of the convective heat transfer coefficient of the raceway in the planetary gear bearing is the highest. When the aperture of the planetary gear is 7 mm and the number of holes is four, the heat transfer performance of the gearbox is the best. The maximum error between the convection heat transfer coefficient fitting formula value and the CFD simulation calculation result is 1.0558 %.

Preventative effects of Lactobacillus plantarum YS-3 on oxazolone-induced BALB/c colitis in mice

Feng, Xia,Zhang, Jing,Qian, Yu,Yi, Ruokun,Sun, Peng,Mu, Jianfei,Zhao, Xin,Song, Jia-Le The Korean Society for Applied Biological Chemistr 2018 Applied Biological Chemistry (Appl Biol Chem) Vol.61 No.3

In this study, the preventative effects of Lactobacillus plantarum YS-3 (LP-YS3) on colitis were studied using an in vitro animal experiment. Oxazolone was administered to BALB/c mice to induce colitis, and the preventive effects of LP-YS3 were determined using serum- and tissue-related indexes. The mice treated with LP-YS3 showed a significant decrease (p<0.05) in disease activity index for colitis by inhibiting colon shortening and increasing colon weight/length ratio. The application of LP-YS3 resulted in a significant reduction in myeloperoxidase, nitric oxide, and malondialdehyde activities and a decrease in glutathione activity (p<0.05) in mouse colon tissues. LP-YS3 also significantly increased serum interleukin-2 and reduced interleukin-10 cytokines levels in mice (p<0.05). Reverse transcription-polymerase chain reaction and western blot assays showed that LP-YS3 application resulted in a significant increase in neuronal nitric oxide synthase, endothelial nitric oxide synthase, c-Kit, and stem cell factor expressions and a decrease in inducible nitric oxide synthase, interleukin-8, and C-X-C motif chemokine receptor 2 expressions in mouse colonic tissues (p<0.05). These findings indicate that LP-YS3 imparts preventative effects on oxazolone-induced colitis in mice.

Phase Transition of As-Milled and Annealed CrCuFeMnNi High-Entropy Alloy Powder

Rui-Feng Zhao,Bo Ren,Guo-Peng Zhang,Zhong-Xia Liu,Jian-Jian Zhang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.09

The CrCuFeMnNi high entropy alloy (HEA) powder was synthesized by mechanical alloying. The effects of milling time and subsequent annealing on the structure evolution, thermostability and magnetic property were investigated. After 50 h of milling, the CrCuFeMnNi HEA powder consisted of a major FCC phase and a small amount of BCC phase. The crystallite size and strain lattice of 50 h-ball-milled CrCuFeMnNi HEA powder were 12 nm and 1.02%, respectively. The powder exhibited refined morphology and excellent chemical homogeneity. The supersaturated solid solution structure of the as-milled HEA powder transformed into FCC1, FCC2, a small amount of BCC and ρ phase in annealed state. Most of the BCC phase decomposed into FCC (mainly FCC2 phase) and ρ phases, and the dynamic phase transition was almost in equilibrium at 900 ℃. The saturated magnetization and coercivity force of the 50 h-ball-milled CrCuFeMnNi HEA powder were respectively 16.1 emu/g and 56.2 Oe.

Laparoscopic Versus Open Surgery for Rectal Cancer: A Systematic Review and Meta-analysis of Randomized Controlled Trials

Zhang, Feng-Wa,Zhou, Zhao-Yu,Wang, Hai-Lin,Zhang, Jv-Xia,Di, Bao-Shan,Huang, Wen-Hui,Yang, Ke-Hu Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background and Aim: Laparoscopic and open rectum surgery for rectal cancer remains controversial. This systematic review compared the short-term and long-term efficiency and complications associated with laparoscopic and open resection for rectal cancer. Materials and Methods: We searched PubMed, Embase, Cochrane Library, ISI Web of Knowledge and the China Biology Medicine Database to identify potential randomized controlled trials from their inception to March 31, 2014 without language restriction. Additional articles were identified from searching bibliographies of retrieved articles. Two reviewers independently assessed the full-text articles according to the pre-specified inclusion and exclusion criteria as well as the methodological quality of included trials. The meta-analysis was performed using RevMan 5.2. Results: A total of 16 randomized controlled trials involving 3,045 participants (laparoscopic group, 1,804 cases; open group, 1,241 cases) were reviewed. Laparoscopic surgery was associated with significantly lower intraoperative blood loss, earlier return of bowel movement and reduced length of hospital stay as compared to open surgery, although with increased operative time. It also showed an obvious advantage for minimizing late complications of adhesion-related bowel obstruction. Importantly, there were no significant differences in other postoperative complications, oncological clearance, 3-year and 5-year or 10 year recurrence and survival rates between two procedures. Conclusions: On the basis of this meta-analysis we conclude that laparoscopic surgery has advantages of earlier postoperative recovery, less blood loss and lower rates of adhesion-related bowel obstruction. In addition, oncological outcome is comparable after laparoscopic and open resection for rectal cancer.

Study on the Social Adaptation of Chinese Children with Down Syndrome

Yan-xia Wang,Shan-shan Mao,Chun-hong Xie,Yu-feng Qin,Zhi-wei Zhu,Jian-ying Zhan,Jie Shao,Rong Li,Zheng-yan Zhao 연세대학교의과대학 2007 Yonsei medical journal Vol.48 No.3

Cordblood-Based High-Throughput Screening for Deafness Gene of 646 Newborns in Jinan Area of China

Shou-Xia Li,Ding-Li Chen,Su-Bin Zhao,Li-Li Guo,Hai-Qin Feng,Xiao-Fang Zhang,Li-Li Ping,Zhi-Ming Yang,Cai-Xia Sun,Gen-Dong Yao 대한이비인후과학회 2015 Clinical and Experimental Otorhinolaryngology Vol.8 No.3

Objectives. Infants with slight/mild or late-onset hearing impairment might be missed in universal newborn hearing screening (UNHS). We identified the mutation hot spot of common deaf gene in the newborns in Jinan area population by screening the mutation spot with neonate cord blood, in order to make clear whether the neonate cord blood for screening is feasible. Methods. Six hundred and forty-six newborns were subjected to both UNHS and genetic screening for deafness by using neonate cord blood. The newborn genetic screening targeted four deafness-associated genes, which were commonly found in the Chinese population including gap junction beta-2 protein (GJB2), gap junction beta-3 protein (GJB3), solute carrier family 26 member 4 (SLC26A4), and mtDNA 12S rRNA. The most common 20 spot mutations in 4 deaf genes were detected by MassARRAY iPLEX platform and mitochondrial 12S rRNA A1555G and C1494T mutations were sequenced using Sanger sequencing. Results. Among the 646 newborns, 635 cases passed the UNHS and the other 11 cases (1.7%) did not. Of the 11 failures, two cases were found to carry homozygous GJB2 p.R143W pathogenic mutation, one case was found to have heterozygous GJB2 235delC mutation, and another one case carried heterozygous GJB3 p.R180X pathogenic mutation. Six hundred and thirty-five babies passed the newborn hearing screening, in which 25 babies were identified to carry pathogenic mutations, including 12 heterozygotes (1.9%) for GJB2 235delC, eight heterozygotes (1.3%) for SLC26A4 IVS7-2A>G, one heterozygote (0.2%) for p.R409H, two homozygotes (0.3%) for m.1494C>T, and two homozygotes (0.3%) for m.1555A>G. Conclusion. Newborn genetic screening through the umbilical cord blood for common deafness-associated mutations may identify carriers sensitive to aminoglycoside antibiotic, and can effectively prevent or delay hearing loss occurs.

The solvent-free modification of butyl rubber in the medium of NaH

Ying Feng,Yan Xia Wang,Hong Qi Shao,En Hua Zhang,Zhen Wang,Jiruo Zhao 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.1

A novel improved butyl rubber (PMIIR) was prepared in a solvent free process, using sodium hydride(NaH) as activating agent and maleic anhydride (MAH) as modifier by processing modificationtechnology in a conventional polymer processing equipment of an internal mixer. The structure ofproducts was characterized by 1H NMR and FT-IR. In this process, polar sodium carboxylate functionalgroup and unsaturated carbon–carbon double bonds were successfully introduced onto the chains of IIRon one step. The reaction mechanism was investigated in detail. It was found that the functionalizationexperienced two modified mechanisms: addition and substitution modified mechanisms. The reactionefficiency and content of MAH were evaluated. Polymer isolation and purification operations werereduced out due to the result of solvent free. Curing and mechanical properties of products wereinvestigated. It was found that PMIIR has greatly increased curing rate due to the incorporation of polargroup and carbon–carbon double bond.