Effects of Different Medium Composition and Exogenous Hormones on Browning of Tree Peony (Paeonia suffruticosa Andr.) Callus in Tissue Cultu

Fang Fang Zhou,Zheng Wang,Li Yun Shi,Jia Jia Niu,Wen Qian Shang,Dan He,Song Lin He 한국화훼학회 2016 화훼연구 Vol.24 No.2

Browning is one of the key factors that influenced the callus subculture of tree peony (Paeonia suffruticosa Andr.). Effects of medium composition and exogenous hormones: macro elements of Murashige and Skoog (MS salts) and iron salt (Fe2+), pH, agar and 6-benzylaminopurine (6-BA), 1-naphthaleneacetic acid (NAA) and kinetin (KT) on the callus browning of P. suffruticosa ‘Shan Hu Tai’ in vitro were studied in this paper. Results showed that the browning of P. suffruticosa callus were more sensitive to KT than 6-BA in different concentrations of 6-BA and KT separately with different concentrations of NAA, and reduced to the lowest (13.3%) under 0.5 mg·L-1 NAA plus 0.3 mg·L-1 KT. 1/4 × MS plus 1/4 × Fe2+ was the best basic medium in which the browning rate was only 18.2%. The browning rate of the callus was the lowest of 4.0% under pH 6.5 and the callus grew better in 7.0 g·L-1 agar than others. This study indicated that the best medium preventing P. suffruticosa callus in vitro from browning was: 1/4 × MS medium supplemented with 6.95 mg·L-1Fe2+, 0.3 mg·L-1 KT, 0.5 mg·L-1 NAA, 6.0 g·L-1 agar and 30 g·L-1 sucrose in pH 6.5.

Slimy partners: the mucus barrier and gut microbiome in ulcerative colitis

Fang Jian,Wang Hui,Zhou Yuping,Zhang Hui,Zhou Huiting,Zhang Xiaohong 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Ulcerative colitis (UC) is a chronic recurrent intestinal inflammatory disease characterized by high incidence and young onset age. Recently, there have been some interesting findings in the pathogenesis of UC. The mucus barrier, which is composed of a mucin complex rich in O-glycosylation, not only provides nutrients and habitat for intestinal microbes but also orchestrates the taming of germs. In turn, the gut microbiota modulates the production and secretion of mucins and stratification of the mucus layers. Active bidirectional communication between the microbiota and its ‘slimy’ partner, the mucus barrier, seems to be a continually performed concerto, maintaining homeostasis of the gut ecological microenvironment. Any abnormalities may induce a disorder in the gut community, thereby causing inflammatory damage. Our review mainly focuses on the complicated communication between the mucus barrier and gut microbiome to explore a promising new avenue for UC therapy.

W. B. Yeats’s Early Poetry of Aging

Zhou Fang 한국예이츠학회 2015 한국예이츠 저널 Vol.48 No.-

W. B. 예이츠의 노년을 다룬 초기 시는, 비록 예이츠가 젊어서 썼지만, 상실 속에서의 위엄이나 폐허에 대한 열정과 같은 기본적인 톤이 생기는데 이것은 그 의 후기 시에서 늙음에 대한 거친 저항으로 발전한다. 그의 초기 시는 후기의 나이에 대한 시의 서곡의 역할을 한다. 더구나, 이 시들은 후기의 나이든 예이츠가 늙음의 족 쇄를 벗고 젊은 자신과 다시 일체가 되게 하는 데 도움이 된다. W. B. Yeats’s early poems on aging, though he wrote them as a young man, have already set the basic tone of dignity amid losses and that of passion for ruins, which is to develop into a wild defiance against aging in his late poems. His earlier poems serve not only as a prelude to the late poems on aging. These poems also help the old Yeats in his late poems liberate himself from the restraints of aging and reunite with the young self.

Knockdown of Chloride Channel-3 Inhibits Breast Cancer Growth In Vitro and In Vivo

Fang-Min Zhou,Yun-Ying Huang,Tian Tian,Xiao Yan Liu,Yong-Bo Tang 한국유방암학회 2018 Journal of breast cancer Vol.21 No.2

Purpose: Chloride channel-3 (ClC-3) is a member of the chloride channel family and plays a critical role in a variety of cellular activities. The aim of the present study is to explore the molecular mechanisms underlying the antitumor effect of silencing ClC-3 in breast cancer. Methods: Human breast cancer cell lines MDAMB- 231 and MCF-7 were used in the experiments. Messenger RNA and protein expression were examined by quantitative realtime polymerase chain reaction and western blot analysis. Cell proliferation was measured by the bromodeoxyuridine method, and the cell cycle was evaluated using fluorescence-activated cell sorting. Protein interaction in cells was analyzed by co-immunoprecipitation. Tumor tissues were stained with hematoxylin- eosin and tumor burden was measured using the Metamorph software. Results: Breast cancer tissues collected from patients showed an increase in ClC-3 expression. Knockdown of ClC-3 inhibited the secretion of insulin-like growth factor (IGF)-1, cell proliferation, and G1/S transition in breast cancer cells. In the mouse xenograft model of human breast carcinoma, tumor growth was significantly slower in animals injected with ClC- 3-deficient cells compared with the growth of normal human breast cancer cells. In addition, silencing of ClC-3 attenuated the expression of proliferating cell nuclear antigen, Ki-67, cyclin D1, and cyclin E, as well as the activation of extracellular signalregulated protein kinases (ERK) 1/2, both in vitro and in vivo. Conclusion: Together, our data suggest that upregulation of ClC- 3 by IGF-1 contributes to cell proliferation and tumor growth in breast cancer, and ClC-3 deficiency suppresses cell proliferation and tumor growth via the IGF/IGF receptor/ERK pathway.

Confinement and Rebellion: Yeats’s Poetry of Aging in the Middle Period

Zhou Fang 한국예이츠학회 2017 한국예이츠 저널 Vol.54 No.-

Complicated by his love for a young girl, Yeats’s poetry of aging in the middle period comprises subtle responses. The poet yearns for the world of passion and youth, which is the release from the confinement of old age, but he is also widely aware of the limitations built both by old age and conventional opinions towards old age. The two contending voices are equally strong, and they form great tension in the poems which later develops into open contention and struggle in Yeats’s late poetry of aging.

Analysis of University Students’ Creativity According toUniversity Level and Gender: Focusing on Business School

Fang Xu Zhou,김채복 사단법인 인문사회과학기술융합학회 2016 예술인문사회융합멀티미디어논문지 Vol.6 No.8

Chinese and Korean economy has combined with each other closely. Economic integration will certainly bring more changes. Analysis of the differences between students' creativity in China and Korea can help two countries to understand each other. This paper presents positive implications for the development of enterprises in both countries and facilitate the friendship of China and Korea.This paper conducts an analysis on the creativity of business school students in China and Korea based on university level and gender. Comparative study with university students ranking among the top 30% and the bottom 30% in China and Korea respectively is performed. ANOVA results show that there are differences among Chinese and Korean students at high and low levels of universities. Also, there are differences among Chinese and Korean male and female students. We find reasons for creativity differences in both countries according to university level and gender.

Absence and Presence: Yeats’s Poetry about the Beloved’s Aging

Zhou Fang 한국예이츠학회 2017 한국예이츠 저널 Vol.53 No.-

예이츠의 늙어가는 애인에 대한 시는 다양한 반응을 보인다. 애인의 늙 어감은 낭만적 환상으로 시작하여, 낭만적 표현을 위한 존재로, 시인이 피하고 부정하 는 감상적 실재로, 언어로 채울 준비가 안된 부재로, 그리고 마침내 살피고, 명상하고, 창조하는 기회가 된다. 늙음은 이제 시의 영감을 위한 생과 사의 문제가 아니라, 시인 이 인정하고, 사색하고, 마침내 변화시키는 엄연한 사실이 된다. 연인에 대한 보다 나 은 앎과 보다 나은 관계와 자신에 대한 이해뿐 아니라 철학적 사색, 사회적 비판, 시적 상상력을 위한 음식이 된다. 애인의 나이듦으로 초래되는 부재는 강한 존재가 된다. 이 를 통해서 사랑에 의존하는 꿈꾸는 시인에서 자신과 자신의 예술의 주인이 된다. Yeats’s poetry about the beloved’s aging manifests a great diversity of response. The beloved’s aging begins as a romantic fantasy, a presence created by the poet for poetic expression, turns into a sentimental reality he tries to evade and to deny, an absence he is not ready to fill with language, and finally becomes an occasion of examination, contemplation, and creation. Aging of the beloved is no longer the life and death for poetic inspiration, but a hard fact the poet accepts, examines, ruminates and finally transforms. It is not only transformed into better knowledge of his beloved, better understanding of their relationship, of himself, but also transformed into food for philosophical contemplation, social critique and finally, poetic imagination. The absence that is caused by the aging of the beloved is turned into a strong presence. With these poetic responses to the aging love, the poet, once a dreamer dependent on love, grows to be a master of himself and of his art.

Association Analysis of Common Genetic Variations in MUC5AC Gene with the Risk of Non-cardia Gastric Cancer in a Chinese Population

Zhou, Cheng-Jiang,Zhang, Liu-Wei,Gao, Fang,Zhang, Bin,Wang, Ying,Chen, Da-Fang,Jia, Yan-Bin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.10

Several lines of evidence suggest that genetic variation in MUC5AC gene might contribute to the risk of gastric cancer. We conducted a case-control study to evaluate the relationship between common genetic variations in MUC5AC gene and non-cardia gastric cancer using an LD-based tagSNP approach in Baotou, north-western China. We genotyped 12 tagSNPs by TaqMan method among 288 cases with non-cardia gastric cancer and 281 normal controls. Unconditional logistic regression was used to calculate odds ratios (ORs) and 95% confidence intervals (CIs) for non-cardia gastric cancer risk in association with alleles, genotypes and haplotypes. We observed that the frequencies of rs3793964 C allele and rs11040869 A allele were significantly lower in cases than in controls. Meanwhile, minor allele homozygotes of rs3793964 and rs11040869 were significantly associated with a decreased risk of non-cardia gastric cancer when compared with their major allele homozygotes. Furthermore, a statistically significantly protective effect of rs885454 genotypes on non-cardia gastric cancer was also observed (for CT vs. CC: OR=0.581, 95%CI=0.408-0.829; for CT/TT vs. CC: OR=0.623, 95%CI=0.451-0.884). Our results indicated that some common genetic variations in the MUC5AC gene might have effects on the risk of non-cardia gastric cancer in our studied population.

Effect of activated carbon modified with oxalic acid on the production of IPA from MX catalyzed by H3PW12O40@carbon and cobalt

Zhou-wen Fang,Hua-jie Liu,Zhi-hao Wang,Di Wen,Xiang-li Long 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.68 No.-

The production of IPA from the oxidation of MX is completed under the catalysis of H3PW12O40 (HPW) loaded on carbon and cobalt. Oxalic acid is tried to modify the carbon to upgrade the catalytic activity of HPW@C catalyst. The experiments show that the best carbon is acquired by carbonizing the carbon at 450 °C for 2 h in N2 after being soaked in a 0.20 mol l−1 oxalic acid solution for 16 h. The IPA produced by the HPW@C catalysts prepared with the carbon modified is 58.9% over that obtained by the catalysts prepared with the original carbon.

Systematic Review and Meta-Analysis of Antibiotic-Impregnated Shunt Catheters on Anti-Infective Effect of Hydrocephalus Shunt

Zhou, Wen-xiu,Hou, Wen-bo,Zhou, Chao,Yin, Yu-xia,Lu, Shou-tao,Liu, Guang,Fang, Yi,Li, Jian-wen,Wang, Yan,Liu, Ai-hua,Zhang, Hai-jun The Korean Neurosurgical Society 2021 Journal of Korean neurosurgical society Vol.64 No.2

Objective : Shunt infection is a common complication while treating hydrocephalus. The antibiotic-impregnated shunt catheter (AISC) was designed to reduce shunt infection rate. A meta-analysis was conducted to study the effectiveness of AISCs in reduction of shunt infection in terms of age, follow-up time and high-risk patient population. Methods : This study reviewed literature from three databases including PubMed, EMBASE, and Cochrane Library (from 2000 to March 2019). Clinical studies from controlled trials for shunt operation were included in this analysis. A subgroup analysis was performed based on the patient's age, follow-up time and high-risk population. The fixed effect in RevMan 5.3 software (Cochrane Collaboration) was used for this meta-analysis. Results : This study included 19 controlled clinical trials including 10105 operations. The analysis demonstrated that AISC could reduce the infection rate in shunt surgery compared to standard shunt catheter (non-AISC) from 8.13% to 4.09% (odds ratio [OR], 0.48; 95% confidence interval [CI], 0.40-0.58; p=0.01; I2=46%). Subgroup analysis of different age groups showed that AISC had significant antimicrobial effects in all three groups (adult, infant, and adolescent). Follow-up time analysis showed that AISC was effective in preventing early shunt infections (within 6 months after implant). AISC is more effective in high-risk population (OR, 0.24;95% CI, 0.14-0.40; p=0.60; I2=0%) than in general patient population. Conclusion : The results of meta-analysis indicated that AISC is an effective method for reducing shunt infection. We recommend that AISC should be considered for use in infants and high-risk groups. For adult patients, the choice for AISC could be determined based on the treatment cost.