Anthocyanin accumulation enhanced in Lc-transgenic cotton under light and increased resistance to bollworm

Xiaoping Fan,Bohong Fan,Yuxiang Wang,Weicai Yang 한국식물생명공학회 2016 Plant biotechnology reports Vol.10 No.1

Breeding of naturally colored cotton fiber has been hampered by the limited germplasm, an alternative way is to use transgenic approach to create more germplasm for breeding. Here, we report our effort to engineer anthocyanin production in cotton. The maize Lc gene, under the control of the constitutive 35S promoter, was introduced into cotton through genetic transformation. Our data showed that the expression of the Lc gene alone is sufficient to trigger the accumulation of anthocyanin in a variety of cell types including fiber cells in cotton. However, the accumulation of colored anthocyanin in cotton fibers requires the participation of light signaling. These data indicate that it is feasible to engineer colored fibers through transgenic approach in cotton. Furthermore, we showed that the Lc-transgenic cotton plants are resistant to cotton bollworm. These transgenic plants are, therefore, potentially useful for cotton breeding against cotton bollworm.

Research on the Evaluation of E-Commerce Logistics in the Cloud Computing Environment

Xiaoping Wang,Jiangang Dong,Jiang Wu,Fan Zhang 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.7

Cloud computing has the advantages of low cost, large scale, general and scalable. In all areas of the social life and the economic life, the cloud computing is widely used. And the emergence of the cloud computing is another revolution in the information society. With the rapid development of e-commerce, the combination of e-commerce and cloud computing has been proved to be a win-win combination. But, there still are many problems of the e-commerce in the cloud computing environment, especially in logistics. In this paper, we establish the e-commerce logistics evaluation system in the cloud computing. And we use the e-commerce logistics evaluation method to evaluate the logistics in the cloud computing based on the improved entropy method. Finally, the experiments show that the proposed method can be effectively applied to the evaluation of e-commerce logistics in the cloud environment and the evaluation results are objective and effective.

Hysteretic performance of a novel composite wall panel consisted of a light-steel frame and aerated concrete blocks

Xiaoping Wang,Fan Li,Liangdong Wan,Tao Li 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.41 No.6

This study aims at investigating the hysteretic performance of a novel composite wall panel fabricated by infilling aerated concrete blocks into a novel light-steel frame used for low-rise residential buildings. The novel light-steel frame is consisted of two thin-wall rectangular hollow section columns and a truss-beam assembled using patented U-shape connectors. Two bare light-steel frames and two composite wall panels have been tested to failure under horizontal cyclic loading. Hysteretic curves, lateral resistance and stiffness of four specimens have been investigated and analyzed. Based on the testing results, it is found that the masonry infill can significantly increase the lateral resistance and stiffness of the novel light-steel frame, about 2.3~3 and 21.2~31.5 times, respectively. Failure mode of the light-steel frame is local yielding of the column. For the composite wall panel, firstly, masonry infill is crushed, subsequently, local yielding may occur at the column if loading continues. Hysteretic curve of the composite wall panel obtained is not plump, implying a poor energy dissipation capacity. However, the light-steel frame of the composite wall panel can dissipate more energy after the masonry infill is crushed. Therefore, the composite wall panel has a much higher energy dissipation capacity compared to the bare light-steel frame.

Selumetinib overcomes gefitinib primary and acquired resistance by regulating MIG6/STAT3 in NSCLC

Xiaoping Song,Lina Wang,Wei Tang,Luyao Yuan,Qingchao Liu,Jing Li,Daidi Fan 대한약학회 2023 Archives of Pharmacal Research Vol.46 No.12

Gefitinib, as the first-generation epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), has achieved great advances in the treatment of non-small cell lung cancer (NSCLC), but drug resistance will inevitably occur. Therefore, exploring the resistance mechanism of gefitinib and developing new combination treatment strategies are of great importance. In our study, the results showed that selumetinib (AZD6244) synergistically inhibited the proliferation of NSCLC with gefitinib. Selumetinib also enhanced gefitinib-induced apoptosis and migration inhibition ability in gefitinib-resistant lung cancer cell lines. Subsequently, the negative regulation between MIG6 and STAT3 was observed and verified through the STRING database and western blotting assays. Sustained activation of STAT3 was significantly downregulated when co-treatment with selumetinib in gefitinib-resistant cells. However, the downregulation of p-STAT3, resulting from the combination of selumetinib and gefitinib was counteracted by the deletion of MIG6, suggesting that selumetinib enhanced gefitinib sensitivity by regulating MIG6/STAT3 in NSCLC. In contrast, p-STAT3 was further inhibited after treatment with gefitinib and selumetinib when MIG6 was overexpressed. Furthermore, the combined administration of selumetinib and gefitinib effectively promoted the sensitivity of lung cancer xenografts to gefitinib in vivo, and the tumor inhibition rate reached 81.49%, while the tumor inhibition rate of the gefitinib monotherapy group was only 31.95%. Overall, MIG6/STAT3 negative regulation plays an important role in the sustained activation of STAT3 and the resistance to EGFR-TKIs. Our study also suggests that EGFR-TKIs combined with MEK1/2 inhibitors, such as selumetinib, may be beneficial to those NSCLC patients who develop a primary or acquired resistance to EGFR-TKIs, providing theoretical support for combining TKIs and selumetinib in clinical cancer treatment.

Investigation on characteristics of pulverized coal dense-phase pneumatic conveying under high pressure

Chen Xiaoping,Fan Chunlei,Liang Cai,Pu Wenhao,Lu Peng,Zhao Changsui 한국화학공학회 2007 Korean Journal of Chemical Engineering Vol.24 No.3

of dense-phase pneumatic conveying of pulverized coal were carried out in a test facility witha conveying pressure up to 4 MPa. The influence of fluidization nitrogen flow rate, the flow rate of supplementarysolid mass flow rate was investigated. Test results indicate that with the increase in fluidization nitrogen flow rate, thesolid mass flow rate increases, and the solids to gas ratio increases at first and then declines. When the fluidization ofpulverized coal in the sending vessel becomes intensive, with the increase in supplementary nitrogen flow rate, thesolids to gas ratio declines and the solid mas flow rate increases. And the solid mass flow rate increases linearly withthe increase in presure diference between two hoppers. The experimental results provide a database for the design

Effect of the grain size on the wear resistance of Al2O3 dispersed Y-TZP ceramics

Liang Xiaoping,Fan Xiaowei,Huai Xuguo,Li Jianxin 한양대학교 세라믹연구소 2010 Journal of Ceramic Processing Research Vol.11 No.3

Using a block-on-ring tribometer with the revolving motion of high chromium cast iron (HCCI) ring against Al2O3 dispersed Y-TZP ceramics (ADZ) blocks, the wear resistance of ADZ ceramics with different grain sizes of ZrO2 was investigated at room temperature. The results show that there was no direct relation found between the grain size and the wear resistance of ADZ ceramics. The tetragonal (t) monoclinic (m) martensitic transformation occurred during the friction process. Only for suitably sized t-ZrO2 particles, the tm martensitic transformation occurred under the action of the frictional stress, and the phase transformation is beneficial for the wear resistance of ADZ ceramics. When the grain size of t-ZrO2 particles is too small,no phase transformation toughening occurred. Moreover, many microcracks will be created by the phase transformation if the grain size is big enough. Then, these microcracks lead to fracture and have a negative effect on the wear resistance of ADZ ceramics.

Alternative Multiplicative Iterative Method for Projection Matrix Design in Compressive Images

Tian Shujuan,Fan Xiaoping,Xie Jingxiong,Li Zhetao,Choi Youngjune,Sekiya Hiroo 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.9

Effects of Dietary Copper Source and Level on Performance, Carcass Characteristics and Lipid Metabolism in Lambs

Cheng, Jianbo,Fan, Caiyun,Zhang, Wei,Zhu, Xiaoping,Yan, Xiaogang,Wang, Runlian,Jia, Zhihai Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.5

An experiment was conducted to determine the effect of dietary copper (Cu) on performance, carcass characteristics and lipid metabolism in lambs. Fifty DorperMongolia wether lambs (approximately 3 month of age; 23.80.6 kg of body weight) were housed in individual pens and were assigned randomly to one of five treatments. Treatments consisted of 1) control (no supplemental Cu), 2) 10 mg Cu/kg DM from Cu-lysine, 3) 20 mg Cu/kg DM from Cu-lysine, 4) 10 mg Cu/kg DM from tribasic copper chloride (Cu2(OH)3Cl; TBCC), 5) 20 mg Cu/kg DM from tribasic copper chloride. The Cu concentration was 6.74 mg/kg DM in the basal diet. Body weight was measured on two consecutive days at the start and the end of the 60-day experimental period. Blood samples were collected and then the lambs were slaughtered on d 60. Performance was not affected (p>0.05) by dietary Cu treatment. Cu-supplemented and control lambs had similar hot carcass weight, dressing percentage and longissimus muscle area, but Cu supplementation, regardless of source and level, reduced (p<0.01) 12th rib backfat and kidney fat in lambs. Plasma tumor necrosis factor-alpha (TNF-) and serum triglyceride concentrations were increased (p<0.05), total cholesterol concentrations were decreased (p<0.05) and nonesterified fatty acids (NEFA) concentrations tended to be increased (p<0.07) by Cu supplementation. However, Serum concentrations of HDL-cholesterol and LDL-cholesterol were not affected (p>0.05) by dietary treatment. Fatty acid profile of longissimus muscle was similar across treatments. These results indicate that Cu-lysine and TBCC are of similar availability in lambs. Cu supplementation given to DorperMongolia wether lambs altered lipid metabolism. The reduction in backfat depth may be due to copper altering TNF- metabolism in lambs. Supplementation of 10 or 20 mg Cu/kg DM showed similar effects on lipid metabolism in lambs.

Parallel numerical analysis of the failure characteristics of earthquake-induced landslides

Zhenzhen Yan,Xiaoping Du,Xiangtao Fan 한국지질과학협의회 2016 Geosciences Journal Vol.20 No.4

Earthquake-induced landslides are serious natural hazards that shocked us with tremendous casualties and great economic losses in many mountainous areas around the world. However, predicting and preventing the earthquake-induced landslides is very difficult due to the complicated relationship between seismic dynamics and coseismic landsliding. Comprehensive understanding of earthquake-induced landslides from the perspective of seismic dynamic mechanism remains inadequate at present. This study employs an elastoplastic spectral element method incorporating parallel computing and represents a realistic three-dimensional slope model via a semi-structured hexahedral mesh to investigate the dynamic failure characteristics of earthquake-induced landslides. Dynamic behaviours of slopes are simulated using a continuumbased approach with a Mohr-Coulomb yield criterion. Displacement fields are calculated using the shear strength reduction technique. Pseudo-static seismic loading is performed to assess the slope stability quantitatively and complex topography is taken into consideration. The Xinzhong landslide that occurred in Beichuan Country is one of destructively collapsing landslides triggered by the Wenchuan earthquake and is therefore selected as a case study for discussion. Three-dimensional visualization of the calculated results quantitatively demonstrated that the three-dimensional numerical model well reproduced the coseismic landsliding response and its essential dynamic failure pattern, which could not be purely captured by Geographic Information Systems (GIS) and Remote Sensing (RS) technologies and calculated using simply two-dimensional numerical model. The numerical results also showed that tensile and shear fractures had significant influences on the nature of the failed surface development. In addition, the presence of seismic loading in the slope could cause obvious disturbances for the slope stability. Comparative analysis indicated that the shear surface of the earthquake-induced slope was shorter and the tension crack surface was deeper than that of the normal gravity condition. Moreover, the landslide mainly occurred in the transition from the upper to lower part of the slope, indicating that the slope topography was one of the crucial factors resulting in slope failure. Although the model was constructed without the presence of a pre-existing failure surface, comparative analysis addressed that the failure surface obtained by the numerical simulation was in close agreement with that by the post-failure investigations. The results could provide insight into better understanding of the relationship between landslide and seismic dynamic mechanism. The study has practical significance for the effective prevention and mitigation of earthquake-induced landslide hazards.

Flow characteristics and dynamic behavior of dense-phase pneumatic conveying of pulverized coal with variable moisture content at high pressure

Liang Cai,Chen Xiaoping,Zhao Changsui,Pu Wenhao,Lu Peng,Fan Chunlei 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.3

Experiments of dense-phase pneumatic conveying of pulverized coal using nitrogen were performed in an experimental test facility with the conveying pressure up to 4MPa and the solid-gas ratio up to 500 kg/㎥. The influences of the total conveying differential pressure, the moisture content, the superficial velocity and the pressure on the mass flow rate and the solid-gas ratio were investigated. Shannon entropy analysis of pressure fluctuation time series was developed to reveal the flow characteristics. Based on the distribution of the Shannon entropy in the different conditions, the flow stability and the evolutional tendency of Shannon entropy in different regimes and the regime transition processes were obtained. The results indicate that the solid gas ratio and Shannon entropy rise with increase in the total conveying differential pressure. A phase diagram and Shannon entropy reveal preferable regularity with superficial velocity. Shannon entropy is different for the different flow regimes, and it can be used to identify the flow regimes. As the moisture content increases, the mass flow rate, the pressure drop and Shannon entropy decrease. Shannon entropy rises with increase in pressure drop.