광양만 유역 퇴적토 중의 다환방향족탄화수소류

정흥호,정호승,김은영,조환익,황주찬,최상원 한국환경과학회 2004 한국환경과학회지 Vol.13 No.6

Polycyclic aromatic hydrocarbons(PAHs) in sediments of Kwangyang bay on Korea, have been investigated by gas chromatography-mass spectrometery (GC/MS). The average content of total PAHs in all samples was 2,211ppb, and the range was from 36 to 22,699pph. The higher concentrations were found in sediment sampled near Kwangyang iron processing plant, Taeindo. The resulting distributions of PAHs according to the number of aromatic ring and molecular ratios of specific aromatic compounds (phenanthrene, anthracene, fluoranthene and pyrene) have been discussed in terms of sample location, origin of the organic matter. Four, five and six rings of PAHs were mainly found near to Kwangyang iron processing plant, and three and four rings near to Yeosu chemical kombinat. This result typically indicated the local characteristics, depending on the origin of PAHs.

Burkholderia cepacia를 이용한 축산폐수의 처리

정팔진,조선영,현미희,김민정,이은주,전민식 한국물환경학회 2004 한국물환경학회지 Vol.20 No.6

We found that the Bacterium Burkholderia cepacia in livestock wastewater treatment plant was predominant species. We investigated the growth rate of this and treatment characteristics for organic matter and nitrogen removal in livestock wastewater using this microorganism. First, we cultured B. cepacia. And then, to conducted treatment for livestock wastewater by using B. cepacia., we changed C/N from 0.2~4.4. When we operated A and B process, changing F/M ratio from 1.2 to 4.4. In experiment of C/N variations, when C/N was 1.8, we found that the optimal condition for organic matter and nutrient removal effect was higher and the removal efficiency of SCOD_(cr), SBOD_5, NH₄-N was 78.4%, 95% and 74.8%. So, It is possible to treat the wastewater having the lower C/N contents such as livestock wastewater using this microorganism. In experiment of A and B process for livestock wastewater, we found that the removal efficiency of organic matter and nitrogen in operating mode of A process was higher than that of B process. Also, the optimal F/M operating A process was 0.013 and the removal efficiency of SBOD_5, SCOD_(cr), TN and TP were 97%, 60%, 95% and 91%.

새로운 알랄아민 항진균제의 합성과 생물학적 평가

정병호,박은주,문현주,유진철 朝鮮大學校 1997 藥學硏究誌 Vol.18 No.2

Some allylamine compounds which are benzothiazole substituants in stead of naphthylring in naftifine, antifungal agents, were sythesized as potential antimycotics. The intermediate Schiff bases that were obtained by condensation of 2-aminobenzothiazole and trans-cinnamaldehyde, were reduced to imine compounds to give allylamines(5a-5d) after mrthylation. These compounds which were tested in vitro against five fungal cell lines containing Trichophyton mentagrophytes. showed no activity in 0.1∼100 μg/ml range.

천연자원을 이용한 간기능 증진제 개발 연구 : Benzo(a)pyrene에 의해 유도된 간기능 장해에 미치는 익모초 추출액의 영향 The Effect of Leonuri Herba on Benzo(a)pyrene Induced Hepatotoxicity

정영건,권오진,윤수홍,박은주 효성여자대학교 부설 한국환경위생연구소 1994 환경위생연구 Vol.4 No.1

Benzo(a)pyrene으로 유도한 rats의 간독성 발현에 미치는 익모초 수침액의 예방 및 치료효과를 실험한 결과 익모초 수침액의 투여는 B(a)P투여로 현저하게 증가된 혈청 및 간장의 AST, ALT, LDH, ALP 활성을 유의성 있게 감소시켰고 B(a)P 투여로 변화한 혈청 지질함량에 약간의 회복도를 보이기는 하나 현저한 차이는 없었다. 즉 익모초의 투여는 B(a)P에 의한 간독성 발현을 효소적으로 유의성 있게 감소시켰으며 그 효과는 전처리가 후처리에 비해 우수하다. The present study was undertaken to compare the pharmacological activity of crude Leonuri Herba reported with the clinical uses in the oriental medicine. Crude Leonuri Herba used for the treatment of gynecologic condition, irregular menstration, parturition hemaostater and diuretics. Therefore we tested the effects of Leonuri Herba water extract on the liver-protective activities in the rats. The results obtained from liver microsomal enzyme assay, measurement of serum and liver alanine aspartate aminotransferase and lipid accumulation indicated that Leonuri Herba extract showed significant liver-pro-tective activities against benzp(a)pyrene poisoning.

창의력 증진을 위한 과학사적 교수·학습 자료 개발

정원우,이우붕,문장수,이일형,김기정,김은주,손동일,방경곤 경북대학교 사범대학부속 중등교육연구소 2007 중등교육연구 Vol.55 No.1

Bisphosphonate가 배양된 치주인대세포의 조골작용에 미치는 효과

김은영,정현주 전남대학교 치과대학 2001 구강과학 Vol.13 No.1

Previous studies have demonstrated an increase in bone mass density with use of bisphosphonate in osteoporosis. This agent acts as an inhibitor of osteoclastic activity and results in increase of net osteoblastic activity. The purpose of the present study was to examine the effect of the bisphosphonate on osteoblastic activity of the human periodontal ligament cells in vitro. Periodontal ligament cells were primarily obtained from extracted healthy third molars. Cells of 4th to 6th passage were cultured in Dulbecco's modified Eagle's medium containing alendronate sodium or etidronate disodium at the concentration of 10^-12∼10^-16㏖/L in 5% CO_2 incubator at 37℃. Cell count and MTT assay for cellular activity were done at 2 to 7 days of culture. Alkaline phosphatase activity at 4 to 7 days of culture and formation of mineralized nodules at 28 days of culture with addition of 50㎍/㎖ ascorbic acid, 10mM β ­ glycerophosphate, 10^-7M dexamethasone were evaluated. 1. Alendronate sodium Compared to the control, the proliferation of periodontal ligament cells was generally increased and the cellular activity was maintained at 2 days of culture and generally decreased at 7 days of culture. Alkaline phosphatase activity of periodontal ligament cells was increased and the formation of mineralized nodules by periodontal ligament cells was enhanced compared to the control. 2. Etidronate disodium The proliferation of periodontal ligament cells increased at 2 days of culture and decreased or maintained at 7 days of culture. Compared to the control, the cellular activity of periodontal ligament cells was generally decreased. Alkaline phosphatase activity of periodontal ligament cells was increased and the formation of mineralized nodules by periodontal ligament cells was enhanced compared to the control. These results suggest that alendronate sodium and etidronate disodium may have a potential effect on osteoblastic lineage of periodontal ligament cells, distinct from their inhibitory action on osteoclasts and could contribute to enhance periodontal regeneration and alveolar bone regeneration.

구강내 Lactobacillus acidophilus V-20 투여시 유산간균과 Porphyromonas gingivalis의 생균수 변화

김은경,정현주 전남대학교 치과대학 2000 전남치대논문집 Vol.12 No.1

The treatment and prevention of periodontitis is focused on the reduction and the elimination of pathogenic bacteria, especially A. actinomycetemcomitans and black pigmented bacteria such as P. gingivalis. To prevent recurrent disease, the recolonization of these bacteria should be inhibited in the periodontal pocket. Since the replacement therapy was introduced in periodontics by Hillman et al, Jeong et al reported that hydrogen peroxide-producing Lactobacillus acidophilus V-20 completely inhibited P. gingivalis and A. actinomycetemcomitans in vitro and mouth gargling with Lactobacillus acidophilus V-20 in periodontitis patients during the maintenance phase improved clinical condition and reduced the No. of P. gingivalis and A. actinomycetemcomitans at 4 weeks of treatment. Prior to replacement therapy with bacteria, dynamics of microbial colonization should be considered. This study was performed to evaluate the change in the viable cell number of Lactobacilli and P. gingivalis after oral administration of L. acidophilus V-20. In periodontal health, gargling increased the No. of Lactobacilli in saliva, buccal mucosa, supragingival plaque from the first week, which maintained for 2-3 weeks after gargling stop, and then returned to the undetectable baseline level at the ninth week. In the periodontal pocket of moderate periodontitis patients, daily irrigation for 1 week and weekly irrigation for subsequent 3 weeks decreased the viable cell number of P. gingivalis during the period of irrigation and increased the number of Lactobacilli, which was maintained from the second to the seventh week. L. acidophilus V-20 was isolated for the first 2 weeks of oral administration, and the 3 different strains of Lactobacilli were isolated continuously for remaining period and identified as L. alimentarius, L. casei subspecies casei and L. fructosus. The first two Lactobacilli strains completely inhibited P. gingivalis in vitro and all the isolated Lactobacilli strains reduced the artificial plaque formation by 55-63%. These results showed that mouth gargling or pocket irrigation with L. acidophilus V-20 increased the No. of intraoral Lactobacilli and caused to decrease in the No. of P. gingivalis. This suggests that the replacement therapy by these Lactobacilli might be useful in the maintenance care of periodontal patients.

광양만으로 유입하는 하천하구에서의 이화학적 특성 : 우수기 직후 유입하천에 따른 영향 effect of inputting rivers after rainy season in summer

김은영,정흥호,정호승,조환익,황주찬,최상원 한국환경과학회 2004 한국환경과학회지 Vol.13 No.9

Physicochemical characteristics of Kwangyang Bay, have been investigated on the effects of inputting rivers just after rainy season in summer 2003. The results ranged from 4.96~8.01 mg/L for DO, 1.16~3.09 mg/L for COD, 7.21~67.71 μM for DIN and 0.12~121.84 μM for phosphate. The COD of Yeosu Area was 1.19~3.09 mg/L, Kwangyang Area was 1.23 ~ 2.76 mg/L. The COD of Wallae cheon in Yeosu Area was 3.09 mg/L and Donghoan in Kwangyang Area was 2.76 mg/L. The reason of high COD might be industrial sewage of organic chemical plants and iron foundry. DIN of Yeosu Area and Kwangyang Area were 7.21--63.43 μM and 8.43 67.71 μM respectively. The average phosphate of Yeosu Area was 23.65 μM. The source of phosphate might be industrial sewages of fertilizer plant and phosphate gypsum stocked with high amounts. The metal concentrations were measured in the range of 1.01~175.10 ㎍/L for Cu, 0.13~1.78 ㎍/L for Cd, 1.13~5.21 ㎍/L for Pb, 1.58~13.57 ㎍/L for Cr and 1.07~20.68 ㎍/L for As. The average Cu concentration was 78.99 ㎍/L in Kwangyang Area. The source of Cu might be industrial sewage of iron foundry. The average Cr concentration of Yeosu Area was 9.07 ㎍/L. That is 2 times higher than Kwangyang Bay 5.74 gg/L, because of sewage of chemical plants.

이온화가스와 응집공정을 조합한 축산폐수의 처리시 용존 유기물의 특성에 관한 연구

이은주,정팔진,김민정,현미희 한국물환경학회 2004 한국물환경학회지 Vol.20 No.6

This study was performed to investigate the variations of hydrophilic and hydrophobic organic matter fractions in soluble organic matter according to livestock wastewater treatment by ionized gas and coagulation effect to these fractions after ionized gas contact. As a result of experiment, because of ionized gas contact, particle in the surface of livestock wastewater was more smaller and the result was consisted of particle size analysis and the amount of small size was increased. Also, we confirmed that organic matters in livestock wastewater by ionized gas contact were removed. The relation equation between ionized gas contact time(X) and TCOD_(cr)(Y) was shown as yscale(y)=3.748-0.431^*xscale(X). That between ionized gas contact time(X) and SCOD_(cr)(Y) was yscale(y)=3.283-0.463^* xscale(X). As respects the HPL(hydrophilic matter)and HPO(hydrophobic matter) fractions of raw in livestock wastewater treatment plant, HPL fraction was 53.2% and HPO fraction was 46.8%. But, HPO fraction according to ionized gas treatment was increased at 30min and after that time, HPL fraction was increased. Also, when we performed coagulation process after ionized gas treatment of raw wastewater, the removal efficiency of organic matter was the highest at 30min of ionized gas treatment because of the variation of HPL and HPO fractions in organic matter by ionized gas. In coagulation process following after ionized gas process, HPO was removed more effective than HPL.

성견의 외과적 치근이개부 골결손에 차폐막과 골이식재를 이용한 조직유도재생술시 치유양상

정은희,정현주 전남대학교 치과대학 1996 전남치대논문집 Vol.8 No.1

The present study was to evaluate the healing patterns of guided tissue regeneration(GTR) using resorbable Vicry??(polyglactin 910) mesh and nonresorbable expanded polytetrafluoroethylene(ePTFE) membrane with or without bone grafting using autogeneous bone and demineralized freeze-dried bone allograft(DFDBA) in the grade Ⅱ furcation defects. Mucoperiosteal flaps were reflected buccally in the mandibular 2nd, 3rd and 4th premolar areas and furcation defects were created surgically by removing 5 × 6mm alveolar bone in 4 dogs. Root surfaces were thoroughly debrided of periodontal ligament and cementum, and notches were placed on root sruface at the most apical bone level. In the right and left mandibular quadrant, each tooth was received Vicryl?? mesh(ACE Surgical Supply Co., USA) only, Vicryl?? mesh with DFDBA, Vicryl?? mesh with autogeneous bone grafts, ePTFE membrane(Gore-tex?? membrane, W.L. Gore & Associates Inc., USA) only, ePTFE membrane with DFDBA or ePTFE membrane with autogeneous bone grafts. For the fluorescent microscopic examination, fluorescent agents were injected at 2, 4 and 8 weeks after surgery. Four weeks after surgery, 2 dogs were sacrificed and ePTFE membranes were removed from remaining 2 dogs, which were sacrificed at 12 weeks after surgery. Undecalcified tissues were embedded in cethylmethacrylate and 10㎛ thick sections were cut in a buccolingual direction. These sections were stained with hematoxylin-eosin stain and Masson's trichrome stain, and evaluated by descriptive histology and linear measurements. The results were as follows : 1) Vicryl?? mesh group showed less connective tissue attachment than ePTFE membrane group. 2) The combination of GTR using Vicryl?? mesh and osseous grafts resulted in new attachment and new bone formation more than GTR using Vicryl?? mesh only. 3) GTR using ePTFE membrane, with or without osseous grafts, enhanced periodontal regeneration. 4) Root resorption and dentoalveolar ankylosis were observed in the areas treated with the combination of GTR and DFDBA. It was suggested that the effect of adjunctive bone grafting in GTR procedure depends on the materials and the physical properties of barrier membranes. Vicryl?? mesh performed a barrier function and the use of adjunctive bone grafting may enhance the periodontal regeneration.