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Cellulase 생산균주의 Screening 및 Bacillus licheniformis CEL-2 유래 Cellulase 특성 규명
권은령 ( Eun Ryeong Kwon ),서정민 ( Jung Min Seo ),이옥진 ( Ok Jin Lee ),김준호 ( Jun Ho Kim ),정영호 ( Young Ho Jung ),김영수 ( Yeong Su Kim ),노홍균 ( Hong Kyoon No ),박창수 ( Chang Su Park ) 한국키틴키토산학회 2015 한국키틴키토산학회지 Vol.20 No.1
We carried out screening of microorganisms producing cellulase from Korean traditional fermented foods. As a result, we isolated 5 strains as cellulolytic microorganisms due to their ability of forming active zone on LB-Agar plate containing carboxymethylcellulose (CM-cellulose) as substrate with trypan blue. Among one of the strains, one strain was selected because its culture supernatant showed the highest cellulase activity for CM-cullulose. In the analysis of 16S rRNA gene sequence from the strain, the isolated strain showed 99% of identity with Bacillus licheniformis, therefore we named the strain Bacillus licheniformis CEL-II. The optimum pH and temperature of cellulase from B. licheniformis CEL-II were the highest activities at pH 5.0 and 60 o C, respectively. In the investigation of cellulase activity for several substrates such as CM-cellulose, alkali swollen cellulose, α-cellulose, Sigma Cellulose and Avicel, the cellulase from B. licheniformis CEL-II showed the highest activity for CM-cellulose. From the analysis of enzyme reaction products obtained from the hydrolysis of alkali swollen cellulose, the cellulase from B. licheniformis CEL-II was characterized to be an endo-type enzyme because the various size cellooligosaccharides were detected as main products on the analysis by thin-layer chromatography.
Kisang Kwon(권기상),Eun Ryeong Lee(이은령),Bo-Kyung Yoo(유보경),Young Hwa Ko(고영화),Hyojung Shin(신효정),Ji-Young Choi(최지영),O-Yu Kwon(권오유) 한국생명과학회 2017 생명과학회지 Vol.27 No.9
우리는 처음으로 쌍별귀뚜라미 소화기관에서 6종류의 미생물을 분리하고 특성을 규명하였다. 이들은 16S rDNA을 기준으로 분류한 결과 4종류(Staphylococcus, Bacillus, Citrobacter, Proteus)에 속하였다. 분리된 6종류의 미생물은 공통적으로 ampicillin에 저항성을 보이지만 kanamycin 저항성은 보이지 않았다. 이들을 Gram염색하여 미생물의 형태적 특징을 확인하였다. Gram-positive한 rod-shaped GL2와 round-shaped GL4는 다른 분리 균 보다 많은 양의 세포외분비물을 만들었다, 이들을 MALDI-TOF-MS spectral analysis결과 87-kDa collagenase, 56-kDa & 200-kDa hypothetical protein이였다. 새롭게 분리된 6종류의 미생물은 귀뚜라미의 생리에 미치는 영향과 이들의 생물공학적 혹은 해충 방제에 이용될 수 있는 연구가 기대된다. We describe the isolation and characterization of six different intestinal microorganisms from the digestive tract of the cricket Gryllus bimaculatus. Based on 16S rRNA gene sequences, we obtained six isolates belonging to four different genera: Staphylococcus, Bacillus, Citrobacter, and Proteus. All the isolates were resistant to ampicillin. Ampicillin is an irreversible inhibitor of the enzymeetranspeptidase, which is needed to make bacterial cell walls. None of the isolates were resistant to kanamycin, which binds to the 30S subunit of the bacterial ribosome and then inhibits total protein synthesis. Gram staining was conducted, in addition to morphological classification under a microscope. Four grampositive isolates and two gram-negative isolates were detected. The gram-positive isolates were GL1 (round shaped, 2 am in diameter), GL2 (rod shaped, 2.5 μm in length), GL3 (rod shaped, 2 μm in length), and GL4 (round shaped, 1.5 μm in diameter). The gram-negative isolates were GL5 (rod shaped, 2 μm in length) and GL6 (rod-shaped, 2.5 μm in length). Notably, two of the isolates, GL2 and GL4, secreted specific extracellular proteins. These were determined by MALDI-TOF-MS spectral analysis to be a 87 kDa collagenase, 56 kDa hypothetical protein, and 200 kDa hypothetical protein. The six isolates in this study could be used for various biotechnological applications and pest management, both in the field and in greenhouse systems. In addition, it would be interesting to determine the relationship between these isolates and their host.
Rat에서 ethylene glycol에 unfolded protein response의 조절
이은령(Eun Ryeong Lee),권기상(Kisang Kwon) 한국생명과학회 2013 생명과학회지 Vol.23 No.9
에틸렌 글리콜(ethylene glycol)은 자동차 부동액 주성분으로 우리 실생활에 널리 쓰이고 있다. 접근이 용이하고 달콤한 맛 때문에 자살목적이나 보관 및 사용 시 의도적으로 또는 실수로 인한 오용사고가 자주 발생한다. 에틸렌 글리콜은 그 자체로는 인체의 독성이 낮지만 생체에서 대사과정을 거치면서 독성이 높아진 유기산을 만들어 다양한 조직에서 광범위한 세포손상을 유발한다. 다양한 세포 스트레스가 소포체(ER) 샤페론과 소포체 스트레스 센서의 유전자 발현을 유도하는 것은 이미 알려져 있다. 본 연구에서는 rat 조직에서 소포체 샤페론과 소포체 스트레스 센서 유전자의 발현 조절이 에틸렌 글리콜에 의해 유도되고, 조직학적 변화도 H&E 염색 및 면역 형광 염색에 의해 확인하였다. Ethylene glycol (EG) is the most commonly used for automotive antifreeze, and it"s easily misuseful for human. EG poisoning occurs in suicide attempts and infrequently, either intentionally through misuse or accidentally because of sweet taste. Though EG itself is mild toxic to the human body, it becomes higher toxic organic acids by in vivo broken down that are responsible for extensive cellular damage in various tissues caused principally by the metabolites. It is already well known that various cellular stresses induce gene expression of endoplasmic reticulum (ER) chaperones and ER stress sensors. This study demonstrated that regulation of gene expression of ER chaperones and ER stress sensors was induced by EG in rat tissues, and in tissues histological changes are also detected by both staining H&E and immunofluorescent.
Short-term Hypothermia Induces Beta-catenin-interacting Protein 1 Gene Expression in PC12 Cells
Kisang Kwon,Bo-Kyung Yoo,Eun Ryeong Lee,Seung-Whan Kim,Kweon Yu,O-Yu Kwon 대한의생명과학회 2015 Biomedical Science Letters Vol.21 No.3
The effects of hypothermic treatment (32℃) on recovery from ischemia are controversial because the precise mechanisms of hypothermia remain unclear. We demonstrated previously that hypothermia induces beta-catenin-interacting protein 1 (CTNNBIP1) gene expression in vitro. In this study, we evaluated the effects of various hypothermic conditions, including lithium chloride treatment, on CTNNBIP1 gene expression. The results show that short-term hypothermic treatment resulted in relatively higher CTNNBIP1 gene expression than that of a longer treatment. These findings indicate that hypothermia controls CTNNBIP1 gene expression, which may provide clues to develop treatments to recover from and diagnose ischemia.
증례 : 순환기 ; 저나트륨혈증에 의한 폐부종을 동반한 동기능부전
류수령 ( Soo Ryeong Ryoo ),김영권 ( Young-kwon Kim ),신성준 ( Sung Jun Shin ),남성은 ( Seong Eun Nam ),오동준 ( Dong Jun Oh ),권기환 ( Ki Hwan Kwon ),차주경 ( Joo Kyoung Cha ) 대한내과학회 2016 대한내과학회지 Vol.90 No.5
저나트륨혈증은 입원 환자에서 비교적 흔히 관찰되며 일반적으로 저나트륨혈증은 심박수 및 전도에 영향이 거의 없는 것으로 알려져 있다. 본 증례에서는 중증도의 저나트륨혈증이 동기능부전을 통한 서맥 동반하였으며 저나트륨혈증을 교정한 후에는 동기능부전이 회복되어 문헌고찰과 함께 보고하는 바이다. Hyponatremia is the most common electrolyte abnormality in hospitalized patients and often presents no symptoms. The association between sinus node dysfunction and hyponatremia has rarely been reported. We describe a 77-year-old woman who developed reversible sinus node dysfunction accompanied by pulmonary edema that was associated with hyponatremia. (Korean J Med 2016;90:444-448)