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Jimenez-Perez, Zuly Elizabeth,Singh, Priyanka,Kim, Yeon-Ju,Mathiyalagan, Ramya,Kim, Dong-Hyun,Lee, Myoung Hee,Yang, Deok Chun The Korean Society of Ginseng 2018 Journal of Ginseng Research Vol.42 No.3
Background: Bioactive compounds in plant extracts are able to reduce metal ions to nanoparticles through the process of green synthesis. Panax ginseng is an oriental medicinal herb and an adaptogen which has been historically used to cure various diseases. In addition, the P. ginseng leaves-mediated gold nanoparticles are the value-added novel materials. Its potential as a cosmetic ingredient is still unexplored. The aim of this study was to evaluate the antioxidant, moisture retention and whitening properties of gold nanoparticles (PgAuNPs) in cosmetic applications. Methods: Cell-free experiments were performed to evaluate PgAuNP's antioxidant and moisture retention properties and inhibition activity on mushroom tyrosinase. Furthermore, in vitro cell cytotoxicity was evaluated using normal human dermal fibroblast and murine B16BL6 melanoma cells (B16) after treatment with increasing concentrations of PgAuNPs for 24 h, 48 h, and 72 h. Finally, in vitro cell assays on B16 cells were performed to evaluate the whitening effect of PgAuNPs through reduction of cellular melanin content and tyrosinase activity. Results: In vitro DPPH radical scavenging assay results revealed that PgAuNPs exhibited antioxidant activity in a dose-dependent manner. PgAuNPs exhibited moisture retention capacity and effectively inhibited mushroom tyrosinase. In addition, 3-(4,5-dimethyl-thiazol-2yl)-2,5-diphenyl tetrazolium bromide results revealed that PgAuNPs were not toxic to human dermal fibroblast and B16 cells; in addition, they significantly reduced melanin content, tyrosinase activity, and mRNA expression of melanogenesis-associated transcription factor and tyrosinase in B16 cells. Conclusion: Our study is the first report to provide evidence supporting that P. ginseng leaves-capped gold nanoparticles could be used as multifunctional ingredients in cosmetics.
Antioxidant Capacity of Venezuelan Honey in Wistar Rat Homogenates
Antonio J. Rodiuez-Malaver,Elizabeth Perez,Patricia Vit 한국식품영양과학회 2006 Journal of medicinal food Vol.9 No.4
The antioxidant effect of several polyphenolic compounds is well known. However, little is known about theantioxidant capacity of Venezuelan honey, which has a high content of polyphenolic compounds. In this work, the antioxi-dant capacity of a genuine honey produced in Mrida, Venezuela was studied using the ferrous iron oxidation with xylenolorange method, the thiobarbituric acid method, and the determination of antioxidant activity. We found that this honey hasthe capacity to decrease significantly the concentration of lipid hydroperoxides and malondialdehyde, produced during thelipid peroxidation process, in a comparable way with other widely studied antioxidants such as melatonin and vitamin E. Itwas found that the antioxidant activity in the 50% honey dilution, the highest concentration we tested, was equivalent to aconcentration of uric acid of 0.62 mM.
광촉매 활성 평가를 위한 더덕의 산화 아연 나노파티클의 One-pot 합성
Hashmoonah Ali,Zuly Elizabeth Jimenez Perez,Ramya Mathiyalagan,Josua Markus,Gokulanathan Anandapadmanaban,허준,Indra Bajikh,양덕춘 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.10
Background : Codonopsis lanceolata is a perennial herb called as ‘Deodeok’ (더덕) in Korea. The roots of C. lanceolate has been reported to have some antioxidant and antimicrobial properties. The chemically reactive saponins of C. lanceolata might be used as a capping agent for the surface of ZnO nanoparticle, ultimately making it a highly efficient photocatalyst. Methods and Results : In this paper, we report the one-pot green synthesis of ZnO nanoparticles via precipitation method using root extract of C. lanceolata. The structure of green synthesized Cl-ZnO NPs was characterized using XRD, EDX, DLS and morphology using TEM. The FT-IR exhibited the information about the functional groups that capped the metal nanoparticle and the formation of metal NPs was confirmed by UV–vis spectra at 356nm. The Cl-ZnO NPs were evaluated for their catalytic activity by measuring the degradation of methylene blue (MB) dye in aqueous solution under UV light (365 ㎚). The result showed efficient degradation of MB, which was degraded 70% within 30 min by Cl-ZnO NPs. Conclusion : This study proves that the green route synthesized ZnO NPs from the root extract of C. lanceolata are low cost, time efficient, bio-degradable and non- toxic. The UVvis spectra confirmed the synthesis of ZnO NPs from C. lanceolata root extract. The Cl- ZnO NPs mediated catalysis exhibited high photocatalysis rate in short time. Ultimately, the green rapid synthesized Cl-ZnO NPs from root extract can be used as an efficient
Antioxidant Capacity of Crude Extracts from Clones of Banana and Plane Species
Antonio J. Rodriguez-Malaver,Elizabeth M. Perez-P?ez,Nayalet Padilla,Gerardo Medina-Ram?ez,Juan Davila 한국식품영양과학회 2006 Journal of medicinal food Vol.9 No.4
Banana and plane are the most important fruits in world trade, behind citric plants. In this work we studiedthe antioxidant capacity of banana and plane varieties of fruits obtained from interspecies crossed varieties of Musa acumi-nataand Musa balbisiana, named Harton plane, Cavendish banana, and Manzano banana. With this purpose we evaluatedbanana and plane crude extracts using the ferrous ion oxidation with xylenol orange method, the thiobarbituric acid method,determination of antioxidant activity, and effect on superoxide anion and hydroxyl radical and the radicals generated by ul-traviolet light. The experiments showed that all extracts have the capacity to decrease the concentrations of lipid hydroper-oxides and malondialdehyde, produced in the lipid peroxidation process, in a manner comparable to that of other widely stud-ied antioxidants like melatonin and vitamin E. Moreover, all extracts had the capacity to inhibit the generation of superoxideanion, hydroxyl radical, and the radicals generated by ultraviolet light. When antioxidant activity was calculated, a value wasfound that was equivalent to a concentration of uric acid between 0.20 and 0.30 mM at the highest concentration of extractused, with uric acid being a potent antioxidant at 1 mM.
Perla Leon-Flores,Nayelli Najera,Elizabeth Perez,Blanca Pardo,Fiacro Jimenez,Dylan Diaz-Chiguer,Francisco Villarreal,Isabel Hidalgo,Guillermo Ceballos,Eduardo Meaney 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.7
Therapeutic approaches to decrease serum triglyceride (TG) concentrations are not successful mainly due to poor adherence or adverse effects of therapies. In consequence, the search for new low-cost and safer therapeutic alternatives is mandatory. Dark chocolate and cacao have shown promising results improving lipid profiles. Recently, using cacao by-products to reduce elevated cardiometabolic risk markers in an animal model of obesity induced by a high-fat diet and fructose, we showed that TGs, low-density lipoprotein cholesterol, and the TG/high-density lipoprotein (HDL) ratio decreased, suggesting that cacao by-products improved the metabolic function of obese animals. Based on these results, as a proof of concept, a blinded placebo-controlled study was implemented to explore the effects of cacao by-products on anthropometric and biochemical variables in a group of overweight subjects participating in a program composed of reduced-calorie-diet counseling plus a simple aerobic exercise plan. The results showed that counseling induced weight and abdominal circumference reductions in both groups. TGs did not change in the control group; however, TG decreased significantly by 54.9 mg/dL (27.9%) in the experimental group. The TG/HDL cholesterol ratio changed markedly (1.5) in the experimental group. The results reported suggest the use of cacao by-products as an alternative for the treatment of hypertriglyceridemia.
Wang, Dan-Dan,Jin, Yan,Wang, Chao,Kim, Yeon-Ju,Perez, Zuly Elizabeth Jimenez,Baek, Nam In,Mathiyalagan, Ramya,Markus, Josua,Yang, Deok-Chun The Korean Society of Ginseng 2018 Journal of Ginseng Research Vol.42 No.1
Background: Ginsenoside F1 has been described to possess skin-whitening effects on humans. We aimed to synthesize a new ginsenoside derivative from F1 and investigate its cytotoxicity and melanogenesis inhibitory activity in B16BL6 cells using recombinant glycosyltransferase enzyme. Glycosylation has the advantage of synthesizing rare chemical compounds from common compounds with great ease. Methods: UDP-glycosyltransferase (BSGT1) gene from Bacillus subtilis was selected for cloning. The recombinant glycosyltransferase enzyme was purified, characterized, and utilized to enzymatically transform F1 into its derivative. The new product was characterized by NMR techniques and evaluated by MTT, melanin count, and tyrosinase inhibition assay. Results: The new derivative was identified as (20S)-$3{\beta},6{\alpha},12{\beta}$,20-tetrahydroxydammar-24-ene-20-O-${\beta}$-D-glucopyranosyl-3-O-${\beta}$-D-glucopyranoside(ginsenoside Ia), which possesses an additional glucose linked into the C-3 position of substrate F1. Ia had been previously reported; however, no in vitro biological activity was further examined. This study focused on the mass production of arduous ginsenoside Ia from accessible F1 and its inhibitory effect of melanogenesis in B16BL6 cells. Ia showed greater inhibition of melanin and tyrosinase at $100{\mu}mol/L$ than F1 and arbutin. These results suggested that Ia decreased cellular melanin synthesis in B16BL6 cells through downregulation of tyrosinase activity. Conclusion: To our knowledge, this is the first study to report on the mass production of rare ginsenoside Ia from F1 using recombinant UDP-glycosyltransferase isolated from B. subtillis and its superior melanogenesis inhibitory activity in B16BL6 cells as compared to its precursor. In brief, ginsenoside Ia can be applied for further study in cosmetics.
In silico and In vitro Analysis of Anti-lung cancer Activity of Compound K in EGF-induced A549 Cells
Muhammad Hanif Siddiqi,Veronica Castro Aceituno,Sungeun Ahn,Shakina Yesmin Simu,Zuly Elizabeth Jimenez Perez,Deok-Chun Yang 한의병리학회 2015 대한동의병리학회 학술대회논문집 Vol.2015 No.10
Ginseng, the root of Pctnax ginseng (P. ginseng), is an oriental traditional herb used in treatment of several human disorder. Ginsenoside, the active compound of P. ginseng have reported as anti-inflammation, anti-osteoporosis and anti-tumour activity. However, the combined in-silico and in-vitro anti-lung cancer activity of compound K (CK) has not been reported so far. Therefore, the purpose of this study was to evaluate the anti-lung cancer activity of CK using in silico docking study followed by in-vitro validation using human lung cancer A549 cells. In recent study we reported that CK could impede growth, and proliferation as well as induce apoptosis in human lung cancer A549 cells. The in-silico study for CK and H-Ras protein was performed by molecular docking simulation. On the other hand, anti-proliferative, anti-migratory and apoptotic activity of C-K were measured by BrdU assay, wound-healing assay and Hoechst-staining respectively. We also evaluated the inhibitory activity of CK on mRNA expression levels of EGFR, H-Ras and ELK1 by realtime PCR (RT-PCR) analysis. Our molecular docking results showed that compared with a control drug (Erlotinib), CK strongly interact with H-Ras protein with good binding energy. Also, our in-vitro results revealed that C-K had the capability to suppress the proliferation, as well as decrease cell migration in A549 cells. Moreover, CK-induced apoptosis and could be blocked mRNA expression level of aforementioned genes in human lung cancer A549 cells. Taken together, these results indicate that CK exerts antiproliferative effects on A549 cells in-vitro and in-silico via EGFR/H-Ras signals.