http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Jimenez-Perez, Zuly Elizabeth,Singh, Priyanka,Kim, Yeon-Ju,Mathiyalagan, Ramya,Kim, Dong-Hyun,Lee, Myoung Hee,Yang, Deok Chun The Korean Society of Ginseng 2018 Journal of Ginseng Research Vol.42 No.3
Background: Bioactive compounds in plant extracts are able to reduce metal ions to nanoparticles through the process of green synthesis. Panax ginseng is an oriental medicinal herb and an adaptogen which has been historically used to cure various diseases. In addition, the P. ginseng leaves-mediated gold nanoparticles are the value-added novel materials. Its potential as a cosmetic ingredient is still unexplored. The aim of this study was to evaluate the antioxidant, moisture retention and whitening properties of gold nanoparticles (PgAuNPs) in cosmetic applications. Methods: Cell-free experiments were performed to evaluate PgAuNP's antioxidant and moisture retention properties and inhibition activity on mushroom tyrosinase. Furthermore, in vitro cell cytotoxicity was evaluated using normal human dermal fibroblast and murine B16BL6 melanoma cells (B16) after treatment with increasing concentrations of PgAuNPs for 24 h, 48 h, and 72 h. Finally, in vitro cell assays on B16 cells were performed to evaluate the whitening effect of PgAuNPs through reduction of cellular melanin content and tyrosinase activity. Results: In vitro DPPH radical scavenging assay results revealed that PgAuNPs exhibited antioxidant activity in a dose-dependent manner. PgAuNPs exhibited moisture retention capacity and effectively inhibited mushroom tyrosinase. In addition, 3-(4,5-dimethyl-thiazol-2yl)-2,5-diphenyl tetrazolium bromide results revealed that PgAuNPs were not toxic to human dermal fibroblast and B16 cells; in addition, they significantly reduced melanin content, tyrosinase activity, and mRNA expression of melanogenesis-associated transcription factor and tyrosinase in B16 cells. Conclusion: Our study is the first report to provide evidence supporting that P. ginseng leaves-capped gold nanoparticles could be used as multifunctional ingredients in cosmetics.
광촉매 활성 평가를 위한 더덕의 산화 아연 나노파티클의 One-pot 합성
Hashmoonah Ali,Zuly Elizabeth Jimenez Perez,Ramya Mathiyalagan,Josua Markus,Gokulanathan Anandapadmanaban,허준,Indra Bajikh,양덕춘 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.10
Background : Codonopsis lanceolata is a perennial herb called as ‘Deodeok’ (더덕) in Korea. The roots of C. lanceolate has been reported to have some antioxidant and antimicrobial properties. The chemically reactive saponins of C. lanceolata might be used as a capping agent for the surface of ZnO nanoparticle, ultimately making it a highly efficient photocatalyst. Methods and Results : In this paper, we report the one-pot green synthesis of ZnO nanoparticles via precipitation method using root extract of C. lanceolata. The structure of green synthesized Cl-ZnO NPs was characterized using XRD, EDX, DLS and morphology using TEM. The FT-IR exhibited the information about the functional groups that capped the metal nanoparticle and the formation of metal NPs was confirmed by UV–vis spectra at 356nm. The Cl-ZnO NPs were evaluated for their catalytic activity by measuring the degradation of methylene blue (MB) dye in aqueous solution under UV light (365 ㎚). The result showed efficient degradation of MB, which was degraded 70% within 30 min by Cl-ZnO NPs. Conclusion : This study proves that the green route synthesized ZnO NPs from the root extract of C. lanceolata are low cost, time efficient, bio-degradable and non- toxic. The UVvis spectra confirmed the synthesis of ZnO NPs from C. lanceolata root extract. The Cl- ZnO NPs mediated catalysis exhibited high photocatalysis rate in short time. Ultimately, the green rapid synthesized Cl-ZnO NPs from root extract can be used as an efficient
Wang, Dan-Dan,Jin, Yan,Wang, Chao,Kim, Yeon-Ju,Perez, Zuly Elizabeth Jimenez,Baek, Nam In,Mathiyalagan, Ramya,Markus, Josua,Yang, Deok-Chun The Korean Society of Ginseng 2018 Journal of Ginseng Research Vol.42 No.1
Background: Ginsenoside F1 has been described to possess skin-whitening effects on humans. We aimed to synthesize a new ginsenoside derivative from F1 and investigate its cytotoxicity and melanogenesis inhibitory activity in B16BL6 cells using recombinant glycosyltransferase enzyme. Glycosylation has the advantage of synthesizing rare chemical compounds from common compounds with great ease. Methods: UDP-glycosyltransferase (BSGT1) gene from Bacillus subtilis was selected for cloning. The recombinant glycosyltransferase enzyme was purified, characterized, and utilized to enzymatically transform F1 into its derivative. The new product was characterized by NMR techniques and evaluated by MTT, melanin count, and tyrosinase inhibition assay. Results: The new derivative was identified as (20S)-$3{\beta},6{\alpha},12{\beta}$,20-tetrahydroxydammar-24-ene-20-O-${\beta}$-D-glucopyranosyl-3-O-${\beta}$-D-glucopyranoside(ginsenoside Ia), which possesses an additional glucose linked into the C-3 position of substrate F1. Ia had been previously reported; however, no in vitro biological activity was further examined. This study focused on the mass production of arduous ginsenoside Ia from accessible F1 and its inhibitory effect of melanogenesis in B16BL6 cells. Ia showed greater inhibition of melanin and tyrosinase at $100{\mu}mol/L$ than F1 and arbutin. These results suggested that Ia decreased cellular melanin synthesis in B16BL6 cells through downregulation of tyrosinase activity. Conclusion: To our knowledge, this is the first study to report on the mass production of rare ginsenoside Ia from F1 using recombinant UDP-glycosyltransferase isolated from B. subtillis and its superior melanogenesis inhibitory activity in B16BL6 cells as compared to its precursor. In brief, ginsenoside Ia can be applied for further study in cosmetics.
In silico and In vitro Analysis of Anti-lung cancer Activity of Compound K in EGF-induced A549 Cells
Muhammad Hanif Siddiqi,Veronica Castro Aceituno,Sungeun Ahn,Shakina Yesmin Simu,Zuly Elizabeth Jimenez Perez,Deok-Chun Yang 한의병리학회 2015 대한동의병리학회 학술대회논문집 Vol.2015 No.10
Ginseng, the root of Pctnax ginseng (P. ginseng), is an oriental traditional herb used in treatment of several human disorder. Ginsenoside, the active compound of P. ginseng have reported as anti-inflammation, anti-osteoporosis and anti-tumour activity. However, the combined in-silico and in-vitro anti-lung cancer activity of compound K (CK) has not been reported so far. Therefore, the purpose of this study was to evaluate the anti-lung cancer activity of CK using in silico docking study followed by in-vitro validation using human lung cancer A549 cells. In recent study we reported that CK could impede growth, and proliferation as well as induce apoptosis in human lung cancer A549 cells. The in-silico study for CK and H-Ras protein was performed by molecular docking simulation. On the other hand, anti-proliferative, anti-migratory and apoptotic activity of C-K were measured by BrdU assay, wound-healing assay and Hoechst-staining respectively. We also evaluated the inhibitory activity of CK on mRNA expression levels of EGFR, H-Ras and ELK1 by realtime PCR (RT-PCR) analysis. Our molecular docking results showed that compared with a control drug (Erlotinib), CK strongly interact with H-Ras protein with good binding energy. Also, our in-vitro results revealed that C-K had the capability to suppress the proliferation, as well as decrease cell migration in A549 cells. Moreover, CK-induced apoptosis and could be blocked mRNA expression level of aforementioned genes in human lung cancer A549 cells. Taken together, these results indicate that CK exerts antiproliferative effects on A549 cells in-vitro and in-silico via EGFR/H-Ras signals.
Markus, Josua,Mathiyalagan, Ramya,Kim, Yeon-Ju,Abbai, Ragavendran,Singh, Priyanka,Ahn, Sungeun,Perez, Zuly Elizabeth Jimenez,Hurh, Joon,Yang, Deok Chun Elsevier 2016 Enzyme and microbial technology Vol.95 No.-
<P>A straightforward synthesis of gold nanoparticles (AuNps) is achieved by novel probiotic Lactobacillus kimchicus DCY51(T) isolated from Korean kimchi via an intracellular membrane-bound mechanism. The bioreduction of HAuCI4 into AuNps was verified by ultraviolet-visible spectrophotometry at similar to 540 nm. AuNps were spherical with varying sizes of 5-30nm. AuNps maintained an average crystallite size of 13 nm and demonstrated long-term stability in physiological buffer and biological media. Furthermore, the protective capping layer consisted of amino acid residues and surface-bound proteins rendered them non-toxic to murine macrophage (RAW264.7) and human colorectal adenocarcinoma (HT29) cell lines. Finally, biosynthesized AuNps served as superior free radical scavengers against 2,2-diphenyl1-picrylhydrazyl (DPPH) in contrast to their corresponding gold salt. In short, this green synthesis is cost-effective and advantageous for the development of a new class of probiotics mediated and nontoxic carriers in targeted drug delivery systems, cancer diagnostic, photothermal therapy, biosensing, and medical imaging. (C) 2016 Elsevier Inc. All rights reserved.</P>