표고재배폐재의 (栽培廢材) 당화에 (糖化) 관한 연구

민두식 ( Du Sik Min ) 한국산림과학회 1979 한국산림과학회지 Vol.43 No.1

In this study, enzymatic hydrolysis of the substrate of the waste wood of Cortinellus edodes was investigated using crude cellulase preparation of Trichoderma viride Pers. ex. Fr. SANK 16374. The crude cellulase was produced by the submerged culture process and produced in the culture fluid was salted out quantitatively by the use of ammonium sulfate. Reducing sugar was determined by the dinitrosalisylic acid (DNS) method. 1. The chemical composition of the waste wood was crude protein 2.26%, c. fat 2.57%, c. fibre 44.60%, c. ash 5.58% and lignin 13.62%. In amino acid composition, no cystine and methionine was showed, but trace amount of Vitamin A, B₁, and B₂, niacine and chloride were detected. (Table 1) 2. As heat treatment of the substrate was found to produce the highest reducing sugar yield being reacted for 48hr, with T.v cellulase, the substrate was heated to 190±5℃. for 45 min, either before or immediately after milling. 3. The substrate heated and ball milled at 190±5℃. for 45 min. the reducing sugar yield reached to 11.5%. 4. The substrate without any treatment was found to produce the highest reducing sugar yield being reacted 72hr. with T. v cellulase, the reducing sugar yield reached to 10.1%. 5. The rate of reducing sugar per each treated substrate was decreased by the order of the substrated, heated and then ball milled at 190±5℃. for 45 min. (11.5%) $gt; without any treatment (10.1)$gt; ball milled and heated at 190±5℃. for 45 min. (6.9%). 6. Saccharification of waste wood has been shown to be possible by heat treated and milling the substrate in contact with cellulase. And it is likely to be recommended that the waste wood may be valuable for raw materials of saccharification.

Cellulase 에 의한 목재당화에 (木材糖化) 관한 연구 - (Ⅲ) 최적 처리조건과 효소처리 잔사의 (殘渣) 재기질화 (再基質化) 효과 -

민두식 ( Du Sik Min ) 한국산림과학회 1980 한국산림과학회지 Vol.49 No.1

In this study, enzymatic saccharification of substrates from Alnus hirsuta Ruper (8-14 years). Quercus acutissima Carruthers, Betula platyphylla var. japonica Nera, Populus euramericana Guiner and Platanus orientalis L. were investigated using crude cellulase preparations of Trichoderma viride Pers. ex. Fr. SANK 16374, and conduced on the optimum treated conditions of the cellulase sacchrification and reactivation of residue of digested substrates. The Trichoderma viride cellulase was produced by the submerged culture process and produced in the culture fluid was salted out quantitatively by the use of ammonium sulfate. The method of dilignification from wood (5 species) was treated by the peracetic acid(PA) method. The reducing sugar was determined by the dinitrosalicylic acid (DNS) method. 1. The results of tests carried out for 96 hr. (Figure 1), show conclusively the initial substrates from 5 species (S,) which has been rendered highly reactive form and the mean rate of reducing sugar was 28.3 %. 2. The results of tests carried out for 96 hr. the reactivation of residue of digested substrates (improvement in the quality of the substrate through preheating in air at 190℃. for 45 min. followed by milling was (60 mesh size) at the same substrate level, increased concentrations of cellulase at the same substrate level, and increased concentrations of cellulase increases the rate of hydrolysis considerably. 3. Figure 1. shows conclusively that the residue of digested substrates (S₁ dried at 60℃) which has been rendered extremly resistant to cellulase action can be reactivated into a highly reactive form (S₂), almost comparable to that of the initial substrates (S₃). And the reducing sugar formation did not show statistically significent differences at 5% levels by initial substrates and the residue of digested substrates (preheating in air at 190℃. for 45 min. fallowed by milling was (60 mesh size)

Cellulase 에 의한 목재당화에 (木材糖化) 관한 연구 - (Ⅰ) 기질 (基質) 처리의 효과 -

정대성,민두식 ( Tae Seong Cheong,Du Sik Min ) 한국산림과학회 1978 한국산림과학회지 Vol.38 No.1

In this study, enzymatic hydrolysis of the holocellulose from Alnus hirsuta (Spach) Rupr. (8-14 yr`s) was investigated using crude cellulase preparations of Trichoderma viride Pers. ex. Fr. SANK 16374. And conducted on the optimum condition of the treated substrate for saccharification. A strain of Trichoderma viride Pers. ex. Fr. SANK 16374 was found to be highly efficient for the cellulase productivity, especially in the submerged culture process. The culture medium used in this experiment was prepared from an extract of wheat bran consisting also of KH₂ PO₄ 10, (NH₄)₂ SO₄ 3, NaNO₃ 3, and MgSO₄7H₂O 0.5g/1. Cellulose powder (Toyo filter paper, 60 mesh) was found to be an importent factar for inducing the cellulase formation. And the cellulase produced in the culture fluid was salted out quantitatively by the use of ammonium sulfate (Fig. 1) Reducing sugar was determined by the Dinitrosalicylic acid (DNS) method, using reagents prepared according to the method of Sumner (1925). The results obtained were summerized as follows; 1. The method of delignification were treated by the Peracetic acid (PA) method, according to the method of Toyama (1970). The yield of holocellulose were decreased in accordance with increasing concentration of Peracetic acid solution; delignification of Alnus hirsuta Rupr. with 20% Peracetic acid was satisfied for 48 hours and 40%∼60% peracetic acid was satisfied for 24 hrs: 2. The substrate (holocellulose) was changed easely into fine powder with enzymatic hydrolysis and cellulase exhibits optimum activity on the reducing sugar formation from substrate at the range of 60-100 mesh. 3. The reducing sugar formation increased in accordance with increasing dry temperature on holocellulose substrate was found to be 190±5℃. 4. The optimal heat treated time of holocellulose substrate was found to be 45 min. for the reducing sugar formation showed the best products. The reducing sugar formation did not show statisticaly significent diflerences at 5% levels by heat treated time for 45 min. and 60 min.

TNF-α 유전자형과 방광암과의 관계

정필두,김은정,엄민식,서정원,윤석중,김종석,이상철,김원재 충북대학교 의학연구소 2001 忠北醫大學術誌 Vol.11 No.2

연구목적: TNF-α는 일부 종양의 종양화 과정과 관련이 있는 것으로 알려져 있다. 본 연구는 TNF-α 발현에 영향을 미치는 TNF-α 촉진자 -308 부위의 유전적 다형성이 방광암과 관련이 있는지 유무를 알고자 시행하였다. 대상 및 방법: 유전자 분석을 위하여 환자 113명 및 대조군 109명으로부터 혈액을 채취하여 genomic DNA를 분리한 후 PCR-RFLP 및 direct DNA sequencing을 통하여 TNF-α유전자의 다형성을 조사하여 방광암의 발생, 병기 및 분화도와 비교 검토하였다. 결과: TNF-α 촉진자 -308 부위의 유전형은 대조군에서는 GG형이 83.5%(90례 및 GA형이 16.5%(19례)로 관찰되었으며 AA형은 없었다. 환자군에서는 GG 형이 85.4%(97례), GA형 및 AA형은 각각 13.1%(15례)및 0.8%(1례)에서 관찰되었다. 두 군 모두에서 GG형이 가장 많이 나타났으며 다음으로 GA형을 보이고 AA형은 1례의 방광암 환자에서만 관찰되었다. -308부위의 경우도 두 군 사이에 유전자형의 차이는 없었다(p=0.259) 분화도별 분포를 보면 grade I이 20례, grade II가 49례, grade Ⅲ은 34례였고 병기별로 표재성인 경우가 90례였으며 침윤성은 14례였다. 분화도가 나빠질수록 GA형이 증가하였다(p=0.04). 그러나 병기와 TNF-α promoter -308부위의 유전자형 사이에는 유의한 상관 관계가 없었다(p=0.123). 결론: 방광암 환자의 혈액에서 GA genotype이 관찰되는 경우, 분화도가 나쁠 가능성이 매우 높기 때문에 좀 더 적극적인 치료와 세밀한 추적관찰을 함으로써 방광암으로 인한 사망과 암의 진행을 예방할 수 있을 것으로 기대한다. Purpose : Tumor necrosis factor-alpha (TNF-α) is involved in tumorigenesis of several cancers as an endogenous tumor promoter. The purpose of this study was to investigate whether genetic polymorphism of TNF-α promoter region (-308) was associated with human bladder tumor. Materials and Methods: The DNA from 113 and 109 respective blood samples of bladder tumor Patients and control group was analyzed by PCR-based restriction fragment length polymorphism (RFLP) and direct DNA sequencing methods to characterize the genetic polymorphism of -308 promoter region of the TNF-α gene in bladder tumor patients. We compared the association of bladder tumor with genetic Polymorphism of TNF-α promoter region(-308) in relation to the stage, grade, recurrence and progressio. Results : Eighty-six percents(97/113) of bladder tumor patients and 83.5% (90/109) of control group showed genotype GG at -308 region of TNF-α. Difference in genetic variations of TNF-α promoter (-308) did not exist between bladder tumor patients and control group(p=0.259). Tumor grade was significantly related to the GA genotype (p=0.04). The higher is the grade in bladder tumor, the more frequent was the GA genotype. Tumor stage, recurrence and progression were not significantly associated with genetic polymorphism of TNF-α promoter region (-308). Conclusion: The GA genotype of TNF-a promoter region (-308) had a significant impact on TNF-α production and was related to higher grade tumor compared to GG genotype. TNF-α serum levels in bladder tumor patients were significantly higher than controls. These data suggested that TNF-α might involve the tumorigenesis of the bladder rather than treatment or prevention of bladder tumor.

전분을 충전한 수성 아크릴레이트 필름의 생분해 특성

김정두,감상규,주창식,이민규 한국환경과학회 2004 한국환경과학회지 Vol.13 No.12

The starch-filled waterborne acrylate (SWAC) films were prepared. The structures and properties of SWAC films were investigated by infrared spectroscopy, thermogravimetric analysis, and strength test. The biodegradability of SWAC film was also studied by determination of reduced sugar products after enzymatic hydrolysis. The surface morphology of the SWAC film was investigated by scanning electron microscopy (SEM). The results showed that the tensile strength and elongation of SWAC film decreased with the increase of starch content. The SWAC film showed significantly higher water absorbed content than waterbonre acrylate film. The biodegradability of SWAC film increased as the content of starch increased. The biodegradation of starch in SWAC film by a-amylase was about 77% of that of pure starch.

합성 Goethite에 의한 인산이온, 황산이온 및 구리이온의 흡착 특성

김정두,유수용,문명준,감상규,주창식,이민규 한국환경과학회 2003 한국환경과학회지 Vol.12 No.9

Adsorption of phosphate, sulfate, and copper ion to goethite was investigated. Goethite was prepared in the alkaline solution. In the single adsorbate systems, the final equilibrium plateau reached within 20 min. The adsorption isotherms of the individual ions could be well described by the Langmuir equation. The maximum adsorption capacities (q_(max)) were calculated as 0.483 mmol/g and 0.239 mmol/g at pH 3 for phosphate and sulfate ion, and 0.117 mmol/g at pH 6 for copper ion, respectively. In competitive adsorption system with phosphate and sulfate, phosphate ion was a stronger competitor for adsorption on goethite than sulfate ion, which was consistent with higher affinity of phosphate ion for the surface compared to sulfate ion. The existence of sulfate ion enhanced the adsorption of copper ion but the adsorption of sulfate was inhibited when copper ion was present.

전분 충전 아크릴레이트 필름의 α-Amylase에 의한 생분해

김정두,유수용,감상규,주창식,이민규 한국환경과학회 2004 한국환경과학회지 Vol.13 No.9

The biodegradability of vinyl acetate acrylate resin and corn starch blend was studied by determination of the reduced sugars produced after enzymatic hydrolysis. The starch hydrolysis reaction by a-amylase was achieved within 5 minutes. Optimal ranges of temperature and pH for the starch hydrolysis by a-amylase were around 80 ℃ and 6.5-7.2, respectively. The biodegradability of the starch-filled acrylate films increased as the content of starch increased. The biodegradation of starch in the starch-filled acrylate film by a-amylase was about 48.6% of that of pure starch. This value of biodegradable starch-filled acrylate film gave a good result with enzymatic shortcut test. The surface morphologies of the starch-filled acrylate film after enzymatic hydrolysis were investigated by scanning electron microscopy (SEM).

설포메틸화법에 의한 無公害 傳統韓紙의 제조에 관한 연구

趙南奭,閔斗植,崔泰鎬,李載源 충북대학교 농업과학기술연구소 1993 農業科學硏究 Vol.10 No.2

This study was performed to investigate the pollution-free papermaking characteristics from paper mulberry by sulfomethylation pulping. Bast fibers were pulped by sulfomethylated cooking liquor and by alkali and alkali-peroxied processes for the comparison. Sulfomethylated pulping resulted in the superior pulp with high yield and better quality compared to those of alkali and alkali- peroxide pulps. Since the pulp was so bright, there was almost no need additional bleaching. Polluttant loads of sulfomethylated pulping effluent were very low in terms of pH, turbidity and color compared to those of alkali. Pollution-free effluents was accomplished by simple filtering treatment with pine bark, charred rice hull and sawdust compost. Pine bark was the most effective in reduction rate of pollutants. Key words : Korean traditional paper, Paper mulberry, Alkali-Peroxide, Sulfomethylated pulping, Effluent.

월악산을 중심으로 한 교육수준별 휴양자원의 이용자 실태

정대성, 민두식, 김병로 忠北大學校 1986 論文集 Vol.32 No.-

-본문참고-

荳科植物 樹葉의 化學的成分

鄭大成,閔斗植,李學州 충북대학교 농업과학기술연구소 1988 農業科學硏究 Vol.6 No.1

This study was Conducted to obtain some fundamental information on the chemical components of leguminous foliage comprising carbohydrates, lignin, extractives, composition of essential elements, pH and protein. Test materials used in this study were the foliage of seven leguminous species. (see, Table 1) Chemical analyses, of organic and inorganic components were performed by TAPPI standard and A.O.A.C. methods. 1.The extractive contents, carbohydrates and lignin of foliage are that the cold water extractives are from 17.7 to 25.37, hot water from 21.2 to 29.7%, 1% NaOH from 41.7 to 65.8%, alcohol : benzen from 6.4 to 12.4%, holocellulose contents from 37.1 to 57.7%, pentosan from 13.6 to 18.8% and lignin from 25.4 to 41.6%. 2.The essential elements of foliage are that the crude carbon contents are from 28.8 to 47.5%, N. from 1.05 to 2.79%, Ca. from 0.56 to 1.47%, Mg. from 0.22 to 0.37%, K. from 1.08 to 4.76%, P. from 0.18 to 0.38%, Cl. from 0.04 to 0.39%, Si. from 0.40 to 2.08% and ash from 4.92 to 8.17%. 3.pH values of the leguminous foliage from 5.19 to 6.82. And crude protein contents are from 10.6 to 18.8% of it.