Evaluation of a Novel Array-Based Toxoplasma, Rubella, Cytomegalovirus, and Herpes Simplex Virus IgG Enzyme Linked Immunosorbent Assay and Its Comparison with Virion/Serion Enzyme Linked Immunosorbent Assays

Dongsheng Wu,Yuanjian Wu,Liuhong Wang,Weidong Xu,Qiao Zhong 대한진단검사의학회 2014 Annals of Laboratory Medicine Vol.34 No.1

Background: The dramatic increase in use of the IgG test for toxoplasma, rubella, cyto- megalovirus (CMV), and herpes simplex virus (HSV) [TORCH] has led to the requirement for a high-efficiency method that can be used in the clinical laboratory. This study aimed to compare the results of BGI-Array ELISA TORCH IgG (BGI-GBI, China) screening method to those of Virion/Serion TORCH IgG ELISA (Virion/Serion, Germany). Methods: Serum specimens (n=400) submitted for routine IgG testing by Virion/Serion ELISA were also tested using the BGI-Array ELISA method. The agreements of these two kinds of method were analyzed by κ-coefficients calculation. Results: Following repeat testing, the BGI-Array ELISA TORCH IgG assays demonstrated agreements of 99.5% (398/400 specimens), 98% (392/400 specimens), 99% (396/400 specimens), and 99.5% (398/400 specimens), respectively. The BGI-Array ELISA IgG as- says provided results comparable to Virion/Serion ELISA results, with κ-coefficients show- ing near-perfect agreement for the HSV (κ=0.87), rubella (κ=0.92) and CMV (κ=0.93) and substantial agreement for the toxoplasma (κ=0.80) IgG assays. The use of the BGI- Array ELISA TORCH IgG assays could reduce the turnaround time (1.5 hr vs. 5 hr by Vi- rion/Serion ELISA for 100 specimens) and were easy to use. Conclusions: BGI-Array ELISA TORCH IgG shows a good agreement with Virion/Serion ELISA methods and is suitable for clinical application.

The vertical distribution and temporal occurrence of three types of rice planthoppers in Shanghai

Wang Dongsheng,Wu Xiangwen,Yuan Yongda,Zhang Tianshu,Shen Huimei,Du Xingbin,Teng Haiyuan,Chang Xiaoli 한국곤충학회 2022 Entomological Research Vol.52 No.1

In this study, we investigated the dynamic occurrence and vertical distribution of three types of rice planthoppers (Nilaparvata lugens, Sogatella furcifera,and Laodelphax striatellus) in Shanghai, China. Our results showed that S. furcifera and L. striatellus infested the lower part of rice plants in the early development stage, S. furcifera and L. striatellus inhabited every part of rice in the middle development stage, and L. striatellus and N. lugens formicated in the upper part of the rice plant in the posterior development stage. The populations of rice planthoppers were larger in July and September, Where more than 600 adult and nymphal planthoppers, per a hundred hills of rice, were found in late July, and out of the 600, the majority were the nymphs of S. furcifera and L. striatellus. S. furcifera was mainly found from July to October, L. striatellus during the rice development, and N. lugens after September, during which their maximal individual number, per a hundred hills of rice, was 480, 220, less than 50, respectively. In addition, our results showed that adult rice planthoppers were mostly observed from late August to mid-October with the highest population being found during mid to late September. The adult S. furcifera, L. striatellus,andN. lugens were mostly observed in August and September, from August to October, and past mid-September, respectively. Moreover, the number of adult L. striatellus that were sticking to yellow sticky card was significantly higher than that of S. furcifera and N. lugens.

A Double-channel Four-band True Color Night Vision System

Yunfeng Jiang,Dongsheng Wu,Jie Liu,Kuo Tian,Dan Wang 한국광학회 2022 Current Optics and Photonics Vol.6 No.6

By analyzing the signal-to-noise ratio (SNR) theory of the conventional true color night vision system, we found that the output image SNR is limited by the wavelength range of the system response λ 1and λ 2 . Therefore, we built a double-channel four-band true color night vision system to expand the system response to improve the output image SNR. In the meantime, we proposed an image fusion method based on principal component analysis (PCA) and nonsubsampled shearlet transform (NSST) to obtain the true color night vision images. Through experiments, a method based on edge extraction of the targets and spatial dimension decorrelation was proposed to calculate the SNR of the obtained images and we calculated the correlation coefficient (CC) between the edge graphs of obtained and reference images. The results showed that the SNR of the images of four scenes obtained by our system were 125.0%, 145.8%, 86.0% and 51.8% higher, respectively, than that of the conventional tri-band system and CC was also higher, which demonstrated that our system can get true color images with better quality.

Prediction of exploration targets based on integrated analyses of source rock and simulated hydrocarbon migration direction: a case study from the gentle slope of Shulu Sag, Bohai Bay Basin, northern China

Changqing Ren,Fugui He,Xianzhi Gao,Dongsheng Wu,Wenli Yao,Jianzhang Tian,Huiping Guo,Yuanxin Huang,Li Wang,Han Feng,Junwei Li 한국지질과학협의회 2019 Geosciences Journal Vol.23 No.6

The Shulu Sag which is a rifted sag with NNE trend is located in the south of Jizhong Depression, Bohai Bay Basin, northern China. The gentle slope and three troughs are situated in the west and east of the sag, respectively. Both of the lower part of Shasan Member (Es3x) and the lower part of Shayi Member (Es1x) act as source rocks in this sag. Researches on the type, quantity, quality and thermal maturity of the respective organic matter have been conducted using Rock-Eval pyrolysis data. Type II is the dominant kerogen in Es1x of all troughs. However, Type II1 and III is the dominant kerogen in Es3x of Middle-Southern and Northern trough, respectively. TOC (total organic carbon) and pyrolysis S2 (hydrocarbon) values suggest that the Es1x source rocks in Middle-Southern and Northern trough are fair to good and poor to fair generative potential of hydrocarbon, separately. The Es3x source rocks in Middle-Southern and Northern trough possess fair to excellent and poor to fair generative potential of hydrocarbon, individually. Tmax (pyrolysis temperature at maximum S2) values indicate that most of Es3x samples are thermally mature, but all Es1x samples are thermally immature. Under large scale condition, the hydrocarbon secondary migration in the upper part of Shasan Member (Es3s), Shaer Member (Es2) and the upper part of Shayi Member (Es1s) have been simulated using fluid potential model with Arcgis 9.3 software. The simulation results reveal the direction of hydrocarbon secondary migration and the distribution of hydrocarbon migration-accumulation units (HMAUS), and also suggest that the hydrocarbon migration direction is obviously controlled by nose-like structure belts where most of hydrocarbons accumulate. That shows high reliability because they are consistent with the hydrocarbon exploration result in this area. On the basis of integrated analyses of source rocks and hydrocarbon migration direction, the following five areas in the gentle slope are identified to be the preferred hydrocarbon accumulation area: Taijiazhuang area, northern and southern Xicaogu area, as well as northern and southern Leijiazhuang area. It is considerably helpful to reduce the risk in hydrocarbon exploration of Shulu Sag.

Apolipoprotein H: a novel regulator of fat accumulation in duck myoblasts

Ziyi Pan,Guoqing Du,Guoyu Li,Dongsheng Wu,Xingyong Chen,Zhaoyu Geng 한국축산학회 2022 한국축산학회지 Vol.64 No.6

Apolipoprotein H (APOH) primarily engages in fat metabolism and inflammatory disease response. This study aimed to investigate the effects of APOH on fat synthesis in duck myoblasts (CS2s) by APOH overexpression and knockdown. CS2s overexpressing APOH showed enhanced triglyceride (TG) and cholesterol (CHOL) contents and elevated the mRNA and protein expression of AKT serine/threonine kinase 1 (AKT1), ELOVL fatty acid elongase 6 (ELOVL6), and acetyl-CoA carboxylase 1 (ACC1) while reducing the expression of protein kinase AMP-activated catalytic subunit alpha 1 (AMPK), peroxisome proliferator activated receptor gamma (PPARG), acyl-CoA synthetase long chain family member 1 (ACSL1), and lipoprotein lipase (LPL). The results showed that knockdown of APOH in CS2s reduced the content of TG and CHOL, reduced the expression of ACC1, ELOVL6, and AKT1, and increased the gene and protein expression of PPARG, LPL, ACSL1, and AMPK. Our results showed that APOH affected lipid deposition in myoblasts by inhibiting fatty acid beta-oxidation and promoting fatty acid biosynthesis by regulating the expression of the AKT/AMPK pathway. This study provides the necessary basic information for the role of APOH in fat accumulation in duck myoblasts for the first time and enables researchers to study the genes related to fat deposition in meat ducks in a new direction.