Effect of Non-animal-Derived Nitrogen Sources on the Production of Hyaluronic Acid by Streptococcus sp. KL0188

Lee, Gil-Yong,Ha, Suk-Jin,Jung, Jong-Hyun,Seo, Dong-Ho,Park, Jong-Yul,Kim, Su-Rin,Park, Nam-Woo,Kweon, Dong-Keon,Park, Sang-Hoo,Park, Cheon-Seok The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.3

Hyaluronic acid (HA) is a linear high-molecular-weight polysaccharide with useful biomedical applications. Streptococcus zooepidemicus, a typical HA-producing bacterium, requires an animal-derived nitrogen source such as tryptone, peptone or sheep blood as a nutrient. Sixteen non-animal-derived (NAD) nitrogen sources were tested as a replacement for the expensive animal-derived nitrogen sources, which may have safety issues. Among the sixteen tested NAD nitrogen sources, a yeast-derived nitrogen source (YE 0251) showed the highest HA productivity, which was equivalent to the control HA production medium containing tryptone in a 5-L jar and in 3,000-L industrial fermentations. In the 3,000-L fermentation, YE 0251 increased cell mass (dry cell weight) and HA production by 11% and 8%, respectively, compared with the control HA production medium. The fmal specific volumetric productivity (0.41 g/L h) was improved by about 70% after reducing the fermentation time from 20 h to 12 h, compared to the conventional production medium.

돼지 심장근 surimi의 기능성에 미치는 locust bean gum 및 xanthan gum의 영향

하정욱,우동균 慶南大學校 附設 工業技術硏究所 1999 硏究論文集 Vol.17 No.-

돼지 심장근으로부터 항산화제(0.02% propyl gallate, 0.2% sodium ascorbate, 0.2% sodium tripolyphosphate) 첨가 세척액에 의해 제조된 돼지 심장근 surimi(5% protein, 0.6M NaCl, 25 mM phosphate buffer solution, pH 6.0)에 sodium alginate(0.4%), calcium carbonate(0.075%) 및 lactic acid(0.6%)를 첨가하여 AC surimi로 조제한 다음, locust bean gum 및 xanthan gum을 각각 0.5% 또는 1.0% 첨가수준에서 얻어진 gel의 보수성과 rheometer에 의한 gel의 물성변화를 측정하여 돼지 심장근 surimi의 가공특성을 검토하였다. AC surimi의 pH에 대한 gum 첨가에 따른 영향을 보면 locust bean gum 또는 xanthan gum 첨가시 경미한 감소경향을 보였고, 1.0% 첨가시에는 0.5%의 경우보다도 약간 낮은 pH값을 나타내었다. Gel의 가열손실에 대해서는 대조구에 비해 크게 감소시키는 결과를 나타내었으며. locust bean gum이 xanthan gum보다도 더욱 효과적이었다. Locust bean gum의 첨가는 보수성을 비롯하여 gel strength. gel hardness. gel springiness 및 gel cohesiveness 등의 물성에 대해 거의 영향을 미치지 않았으나, xanthan gum 첨가구는 이들 물성 중에서 보수성의 현저한 gel hardness의 경미한 증가추세만이 locust bean gum과 차이를 보였다. Pork heart surimi was prepared by repeated washings in the presence of antioxidants such as propyl gallate(0.02%), sodium ascorbate(0.2%), and sodium tripolyphosphate(0.02%). Changes in pH, cooking loss, water holding capacity and rheological properties such as strength, hardness, springiness and cohesiveness of gels prepared from alginate and calcium added Pork heart surimi(AC surimi) with 0.5% and 1.0% of locust bean gum or xanthan gum were investigated. The pH values and cooking losses were lower than those of the AC control by the addition of locust bean gum or xanthan gum. Xanthan gum resulted in a remarkable increase of water holding capacity and a little increment of gel hardness, whereas locust bean gum resulted in little effect on water holding capacity. gel strength, gel hardness, gel springiness and gel cohesiveness.

돼지 심장근 surimi의 기능성에 미치는 kappa-carrageenan과 guar gum의 영향

하정욱,우동균 慶南大學校 附設 工業技術硏究所 1998 硏究論文集 Vol.16 No.-

돼지 심장근으로부터 항산화제(0.02%) prppyl gallate, 0.2% ascorbic acid, 0.2% sodium tripolyphosphate) 첨가 세척애세 의해 제조된 surimi(5% protein, 0.6 M NaCl, 25mM 인산완충액, pH 6.0)로써 sodium alginate(0.4%), calcium carbonate(0.075%) 및 lactate(0.6%) 의 첨가에 의해 AC surimi로 조제한 다음, kappa-carrageenan 및 guar gum의 0.5% 또는 1.0% 첨가 수주에서서 얻어진 gel의 보수성과 가열손실을 비롯하여 gel 의 물성을 측정하였다. AC surimi의 pH 에 미치는 두 가지 gum의 영향은 경미하였으며, 의 가열손실에 대해서는 Kappa-carrageenan 첨가구가 guar gum 첨가구에 비해 매우 낮은 경향을 보였고, 보수성을 비롯하여 gel strength 및 gel hardness 등의 측정결과는 kappa-carrageenan은 상당히 큰 증가를 보였으나 gua gum에서는 거의 영향이 나타나지 않았다. Gel springiness에 대해서는 감소경향을 나타내었고, gel cohesiveness는 guar gum첨가구에서만 다소 증가되는 경향을 나타내었다. Pork heart surimi was prepared by repeated washing in the presence of antioxidants such as propyl gallate(0.02%), ascorbic acid(0.2%) and sodium tripolyhosphate(0.2%). Changes in cooking loss, water holding capacity and rheological properties such as strength, hardness, springiness and cohesiveness of gels prepared from alginate/calcium added pork heart surimi(AC surimi) with 0.5% and 1.0% of kappa-carrageenan(KC) or guar gum(GG) were determined. The pH level of AC surimi with KC(0.5%) and GG(1.0%) was nearly the same compared with that of the AC control, and KC(1.0%) lowered the pH level of 0.2 unit. KC resulted in lower cooking loss than GG, but KC increased water holding capaciy, gel strength and gel hardness of AC surimi whereas guar gum resulted in little effect on those properties. KC decreased gel cohesiveness of AC gel, and guar gum showed higher gel cohesiveness than KC.

유자 중 limonoid 화합물의 정량 및 분리정제에 관한 연구

우강용,하정욱,우동균 慶南大學校 附設 工業技術硏究所 1997 硏究論文集 Vol.15 No.1

남해안에서 생산되는 유자의 씨와 과피중의 limonoid 함량을 HPLC (Spectra Physics 8800, U.S.A)에 의하여 분석 정량해 보았다. Limonoid 화합물 중 limonin과 nomilin은 methanol과 acetone에 의해 비교적 추출이 용이하였으며, acetone과 acetonitrile에 의해서 naringin이 거의 추출되지 않았으나 methanol에 의해서는 상당히 많은 양이 추출되었다. 유자씨에는 107.4mg%의 limonin과 85.39mg%의 nomilin이 함유되어 있으나, 유자 과피중에는 0.05mg%의 limonin과 0.034mg%의 nomilin이 함유되어 있었다. Amberite XAD-2 칼럼상에서 naringin 이 초기에 용출된 다음 100∼150㎖ 사이에서 limonin이 그리고 200∼250㎖ 사이에서 nomilin이 각가 최대의 용출율을 나타냄으로서 limonin과 nomilin의 분리정제 가능성이 확인되었다. Seeds and sarcocarp of citron from the southern part of Korean peninsula were analyzed for limonoid content by HPLC (Spectra Physics 8800, U.S.A) with the Nova-pak C18 column. Limonin and nomilin were well extracted by methanol and acetone, respectively, and little naringin was extracted by acetone or acetonitrile, but extracted in great quantity by methanol. Citron seeds contained 107.4 mg% of limonin and 85.39 mg% fo nomilin, and citron sarcocarp had 50 ppm of limonin and 34 ppm of nomilin. On the Amberite XAD-2 column naringin was elured at first, and then limonin and nomilin were eluted between 100㎖ and 150㎖, and between 200㎖ and 250㎖, respectively.

자가 골수 중간엽줄기세포와 생분해성 고분자 스캐폴드를이용한 조직공학적 인공혈관 제조

조승우,김동익,박희정,임상현,전오주,김수현,김영하,최차용,이민재,김종성,장인성,김병수 대한혈관외과학회 2003 Vascular Specialist International Vol.19 No.2

실크 피브로인의 대규모 투석 공정의 단위 모듈의 개발

김동우,하성진,임건빈,허원 江原大學校 産業技術硏究所 2001 産業技術硏究 Vol.21 No.B

For the development of a large-scale dialysis process for silk fibroin solution, a batch and continuous dialysis chamber was designed and built, which are to be used as a unit module for the process. A series of dialysis experiments were carried out to estimate the kinetic parameters for dialysis using the solutions of salt-solubilized silk fibroin. The solution was dialyzed by distilled water either batch-wise or continuously. Dialysis kinetics was monitored by measuring refractometery at high salt concentration and conductivity at lower salt concentration. The apparent dialysis coefficients were 5.4g/m2-hr and 1.8g/m2-hr, for well mixed batch and continuous dialysis, respectively. The alteration of molecular weight distribution of dialyzed silk fibroin solution was monitored.

탈지 환원유에서 Bifidobacterium longum의 호기성 고농도 배양에 관한 연구

구동환,하상우,조수현,박기문,김동운,강국희 성균관대학교 생명과학자원연구소 1997 生命資源科學硏究 Vol.4 No.1

The objectives of this study were to investigate the optimal condition for growth and highly concenterated culture of Bifidobacterium longum (Bif longum). The effects of additives were compared with titratable acidity and viable cell counts, and 10% reconstituted skim milk (no additive) was used as a control. Correlation coefficient for the growth of Bif. longun between CO_2 anaerobic jar method and overalaid medium method was R=0.99. When 1% Bif. longurn was inoculated on 12%, 14%, and 16% reconstituted skim-milk, viable cell counts were 8.60, 8.89, and 8.93 Log CFU/ml, respectively. When Lactose, Glucose, Glucosamine, Fructose, Peptone, N-acethyl-D-glucosamine, D-galactose, and liver extract were added to 16% reconstituted skim-milk, the viable cell counts were 8.61, 9.05, 9.15, 9.24, 8.82, 9.75, 9.17, and 9.64 Log CFU/ml, respectively. Therefore, D-galactose, liver extract, and N-acethyl-D-glucosamine were selected as final additives. When Bif longum was inoculated on Fermentor-I (16% reconstituted skim milk containing 0.1% yeast extract, 0.5% D-galactose and 0.5% liver extract), Fermentor-II (16% reconstituted skim milk containing 0.190% yeast extract, 0.590% N-acethyl-D-glucosamine and 0.5% liver extract), Batch culture-I (16% reconstituted skim milk containing 0.1% yeast extract, 0.5% D-galactose and 0.5% liver extract), and Batch culture-II (16% reconstituted skim milk containing 0.1% yeast extract, 0.5% N-acethyl-D-glucosamine and 0.5% liver extract), viable cell counts were 10.46, 9.37, 9.03, and 8.83 Log CFU/ml, respectively. Although liver extract was the important additive for the highly concentrated culture of Bif. longum in this study, it caused serious off-flavor when liver extract-1 (contained cell mass) was added to Fermentor culture. However, flavor was improved when liver extract-2 which was removed cell mass, was used instead of liver extract-1. Addition of 2% liver extract-II and 1% D-galactose had the similar effect with addition of 0.5% Liver extract-I and 0.5% D-galactose on the growth of Bif. longum.

대장암종에서 암관련 유전자들의 Promoter Methylation과 암종 발생 및 진행과의 관게에 대한 연구

정동준,이정은,민영기,조성우,백무준,양승하,김의한,김창진 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

The colorectal corcinoma is one of the most common malignant tumor in Korea and its carcinogenic mechanism has not been elucidated exactly yet. So far, the most common carcinogenic mechanism has been known to be multiple genetic changes. But, the CpG island hypermethylation has been revealed to be one of the most important carcinogenic mechanism second to the genetic changes. The epigenetic changes characteristically imprint and transfer to the next generation without changes of nucleotide sequences. When the CpG island of promoter or 5'- exon 1 of a specific gene is methylated by DNA methyltrasferases (DNMTs), the gene expression is inhibited. Carcinogenic mechansisms by promoter methylation has been well known, but the progression of the cancer has not been elucidated yet. The molecular researches on the carcinogenic mechanisms largely depended on the tumor tissue containing various components of cell, due to difficulties in harvesting pure homogeneous tumor cells. Promoter methylatonof the cancer related genes as APc, hMLH2, p16, DAP-K and MGMT in 50 cases of sporadic colorectal carcinoma was studied. The pure normal colorectal epithelial cells, and cancer cells from superficial and deep invasive portion were harvested respectively by laser captured microdissection(LCM), and DNA was extracted from them. The promoter methylation was studied by methylation specific poymerase reaction (MSP) after bisulfite modification of the DNA and the expression of the genes by immunohistochemistry. The results suggests that promoter methylation of the cancer related genes are closely related with the colorectal carcinogenesis and it occurrs from early phase of carcinogenesis, progressing constantly to the late stage. And the promoter methylation of normal epithelial cells increased by age. The p15, known to be the genes related to hematologic malignancy, was closely related to the colorectal carcinoma as well.

농가용 저온저장고의 제어변수에 따른 성능평가

김석우,김성만,현명택,최동호,고정삼,박영하 제주대학교 공과대학 첨단기술연구소 2001 尖端技術硏究所論文集 Vol.12 No.2

The low temperature storage method has been used to increase the value of agricultural products by reducing quality deterioration and controlling respiration rate. The respiration rate of agricultural products depends on several factors such as temperature, moisture, gas composition and microbe inside the storage room. Among that temperature is the most important factor, which affects respiration rate and causes severe damage. The results obtained from these experiments are that electric consumption is decreased as setting temperature is decreasing and temperature deviation is increasing. In the ventilation experiment, the case ventilated four times a day(every six hours a day) with ten minutes operation each time, was inspected require as much electric consumption as non-ventilation case.

저장물의 적재방법에 따른 저온저장고의 열성능 분석

김석우,김성만,현명택,최동호,고정삼,박영하 제주대학교 공과대학 첨단기술연구소 2001 尖端技術硏究所論文集 Vol.12 No.2

The heat performance in a forced circulating cool storage room was numerically investigated. A commercial PHOENICS package was used to simulate 3-D airflow. For solving the governing equations a standard k-ε turbulent model was implemented to calculate steady state turbulent velocity and temperature distributions. The results show that the large air contacting area of products increases the storage quality, because the airflow between products helps to remove the heat from the products by convective heat transfer.