대황황련해독탕의 항고지혈증 작용

김영석,정은아,장종철,양형길,김남재,조기호,배형섭,이경섭,김동현 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2001 No.-

Whangryunhaedok-Tang (WT) is formulated with Coptidis Rhizoma, Phellodendri Cortex, Scutellariae Radix and Gardeniae Fructus, and Daewhang-whangryunhaedok-Tang (DWT) is made by the combination of Rhei Rhizoma, a wellknown anticostipation drug in WT. Therefore, DWT has been evaluated for antihyperlipidemic effects on experimental hyperlipidemic rats and mice induced by corn oil and high cholesterol-diet. Oral administration of DWT significantly inhibited the increase of serum triglyceride and LDL-cholesterol levels, and the decrease of serum HDL-cholesterol levels in hyperlipidemic rats induced by corn oil. Also, oral administration of DWT significantly prevented the increase of serum total cholesterol, triglyceride and LDL-cholesterol, and liver total cholesterol and triglyceride in 1% cholesterol-diet fed mice. These results suggest that DWT is effective for the treatment of hyperlipidemia.

대황황련해독탕의 사염화탄소 유발 간장해 보호효과 미치 급성독성

김영석,정은아,장종철,양형길,김남재,조기호,배형섭,이경섭,김동현 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2002 東西醫學硏究所 論文集 Vol.2002 No.-

ABSTRACT - This study was performed to evaluate hepatoprotective effect of daewhang-whangryunhaedok-Tang(DWT) on liver injured rats induced by CCI_4 and the acute oral toxicity of it in mice. The activities of serum transaminase(ALT/AST), alkaline phosphatase(ALP) and lactic dehydrogenase(LDH), the levels of serum total cholesterol(TC) and triglyceride(TG), change of liver enlargement, and inhibitory activities of lipid perotidation, catalase and glutathione-S-transfrease(GST) in liver microsome were determined in hepatotoxic rats induced by CCI_4. DWT was significantly reduced the serum ALT, AST, ALP, LDH. TC and TG lecels. And, the increase of lipid peroxidation, decrease of catalase and GST activities in the liver microsome of CCI_4-intoxicated rat were significantly improved by the treatment of DWT. Male and female mice were administered maximum dosages of 5.000 mg/kg b.w. of DWT. After single oral administration of DWT to mice, we observed them daily for 2 weeks.DWT did not induce any toxic signs in the mortalitie, clinical signs, body weight changes, and gross necropsy finfings of mice. Based in these results. It is concluded that DWT may have the hepatoprotective effect on CCI_4 induced hepatotoxicity in rats. Also. DWT may have no side effect and its LD_50 value may be over 5.000mg/kg b.w. in mice.

Lidocaine, Thrombin, Epinephrine 의 항균효과

김진우,이동건,전혜선,김승준,김석찬,안중현,김치홍,권순석,김영균,김관형,문화식,신완식,송정섭,박성학 대한감염학회 2005 감염과 화학요법 Vol.37 No.6

목적 : 기관지내시경 검사에서 흔히 사용되는 국소마취제인 lidocaine과 내시경시 지혈목적으로 사용되는 thrombin과 epinephrine이 각종 균주에 미치는 항균효과에 대해 알아보고자 하였다. 재료 및 방법 : 균주는 가톨릭대학교 성모병원에서 2004년 3월부터 2004년 9월까지 임상검체 에서 동정된 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 각각 42, 42, 42, 43주를 대상으로 하였다. Lidocaine, thrombin, epinephrine 감수성 검사는 National Committee for Clinical Laboratory Standards (NCCLS, 2002)의 기준에 따랐다. 결과 : Lidocaine은 S. aureus, S. pneumoniae, P. aeruginosa에서 MIC_(50), MIC_(90) 모두 20,000 ㎍/mL 이었다. K. pneumoniae는 각각 10,000 ㎍/mL이었다. Thrombin은 S. aureus와 P. aeruginosa에서 MIC50 500 lU/mL 과 MIC_(90) 500 IU/mL 이상이었고, K. pneumoniae에서는 MIC_(50)과 MIC_(90)이 모두 500 lU/mL이상이었으나 S. pneumoniae에서는 MIC_(50)과 MIC_(90)은 125 IU/mL이었다. Epinephrine은 K. pneumoniae, S. pneumoniae에 대한 MIC_(50), MIC_(90)가 모두 >500 ㎍/mL이었고, S. aureus와 P. aeruginosa에 대한 MIC_(50), MIC_(90)가 모두 500 ㎍/mL이었다. 결론 : 기관지 내시경 검사에서 흔히 쓰이는 lidocaine, thrombin, epinephrine 등의 약제들이 호흡기 질환의 흔한 병원균인 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 균주들에 대해 항균 효과가 있을 수 있어 세균배양검사에 영향을 미칠 수 있겠다. Background : We performed this stody to find out about antimicrobial effect of lidocaine which is commonly used local anesthetic, and thrombin and epinephrine used for hemostasis during bronchoscopic procedures. Materials and Methods : The microorganisms that were cultured from specimens obtained during bronchoscopy were Staphylococcus aureus (n=42), Streptococcus pneumoniae (n=42), Klebsiella pneumoniae (n=42), and Pseudomonas aeruginosa (n=43) collected from St. Mary's Hospital, from March to Sep 2004 were used for susceptibity testing. Susceptibility to lidocaine, thrombin, and epinephrine were tested according to the National Committee for Clinical Laboratory Standards. Result : MIC_(50) and MIC_(90) of lidocaine for S. aureus, S. pneumoniae, P. aeruginosa were all 20,000 ㎍/mL and that for K. pneumoniae were 10,000 ㎍/mL. MIC_(50) and MIC_(90) of thrombin for both S. aureus and P. aeruginosa was 500 IU/mL and above 500 IU/mL, respectively; that for K. pneumoniae were all above 500 IU/mL and for S. pneumoniae they were 125 IU/mL, MIC_(50) and MIC_(90) of epinephrine for K. pneumoniae and S. pneumoniae were above 500 ㎍/mL; that for S. aureus and P. aeruginosa were 500 ㎍/mL. Conclusion : We observed possible antimicrobial effect of lidocaine, thrombin, and epinephrine in vitro against pathogens such as S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa, which are common respiratory microorganisms. The use of these agants could affect the result of bacterial culture.

HAZARD ANALYSIS USING MULTI-COMPONENT ANALYSIS IN CHEMICAL PROCESSES

Kim,Ku-Hwoi,Lee,Dong-Eon n,Kim,Yonhg-Ha,Yoon,En-Sup 한국화재소방학회 2001 한국화재소방학회 학술대회 논문집 Vol.2001 No.추계

두개외 경동맥 협착증에 대한 스텐트 삽입술의 추적 결과

윤영섭,홍범기,최동훈,김선호,김동익,김승민,장양수,심원흠 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.11

持續的 農業體系 分析

金東燮,金大熙 公州大學校 地域開發硏究所 1995 地域開發硏究論叢 Vol.3 No.1

Summary The interest of sustainable agricultural system (organic agricultural system in Korea) is getting popular because of serious environmental pollution. This paper was designed with the intention of improving the understanding levels of sustainable agricultural system by comparing the characteristics of sustainable agricultural system with chemical agricultural system. The main point of sustainable agricultural system is that current agricultural system adapts to an ecosystem for nature conservation. This it would be completed with changing of attitudes and values of agriculture and with much interest and study of sutainable agricultural system. The interest of sustainable agricultural system (organic agricultural system in Korea) is getting popular because of serious environmental pollution. This paper was designed with the intention of improving the understanding levels of sustainable agricultural system by comparing the characteristics of sustainable agricultural system with chemical agricultural system. The main point of sustainable agricultural system is that current agricultural system adapts to an ecosystem for nature conservation. Thus it would be completed with changing of attitudes and values of agriculture and with much interest and study of sustainable agricultural system.

GH_3 세포(rat somatomammotropic tumor cell)에서 TRH가 Phospholipase D 활성에 미치는 효과

김동선,김태화,이창범,안유헌,윤미섭,한중수 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.4

연구배경: GH_3 세포는 TRH에 반응하여 세포막의 수용체와 G 단백, PI-PLC, PKC를 활성시켜 성장호르몬 및 프로락틴을 분비한다고 알려져 있다. PLD는 phosphatidylcholine을 phosphatidic acid(PA)와 choline으로 가수분해하는 효소로서 세포의 증식과 호르몬 분비에 관여한다. 본 연구는 GH_3 세포에서 아직 알려지지 않은 TRH의 PLD 활성에 대한 영향을 규명하고자 하였다. 방법: GH_3 세포를 1.5×10^6씩 분주하고 [^3H] myristate로 표지한 다음에 0.3% 알코올로 전 처치하였다. TRH 등의 시험제를 처치한 후에 메탄올로 반응을 종결하고 세포에서 총 지질을 추출하였다. PLD 활성은 박층크로마토그래피를 이용하여 총 [^3H] phospholipid에서 [^3H] phosphatidylethanol의 비율로 구하였다. 결과: TRH (1μM)의 처치 시에 PLC 활성은 44배 증가하였다. PLD 활성은 TRH (1μM), mastoparan (5μM), PMA(500nM)를 30분간 처치 시에 각각 1.9, 1.5, 2.2배 증가하였다. TRH(1μM)의 시간에 따른 PLD 활성 변동은 15, 30, 60, 120, 240분에 각각 142%, 170%, 172%, 160%, 115%의 증가를 보였다. 결론: GH_3 세포에서 TRH의 호르몬 분비와 세포증식의 신호전달 기전으로서는 PLC 활성뿐 아니라 PLD의 활성도 관여함을 시사한다. Backgroud: GH_3 cells are a well characterized and widely used model used for the in vitro study of growth hormone (GH) secretion. Thyrotropin releasing hormone (TRH) binds to receptors belonging to the family of G protein-coupled receptors, and secrets both GH & prolactin. Phospholipase D (PLD) is and enzyme that hydrolyses phosphatidylcholine to yield phosphatidic acid and choline, and plays important roles in cellular proliferation and hormonal secretion. To elucidate the pathway of the action of TRH in GH_3 cells, we investigated the activities of PLC and PLD in GH_3 cells treated with TRH or phorbor 12-myristate 13-acetate (PMA). Methos : GH_3 cells were labeled with [^3H] myristate, followed by incubation of with 0.3% ethanol, prior to before the addition of the agonists. The total lipids were extracted from the harvested cells following treatment with the agonists. The PLD activity was assessed by measuring [^3H] phosphatidylethanol from the [^3H] phospholipid using thin layer chromatography. Results : TRH (1μM) stimulated the PLD activity by 44-fold over that of the control values. TRH (1μM), mastoparan (5μM), and PMA (500μM) for 30 minutes increased PLD activity by 1.9, 1.5 and 2.2 fold, respectively, in comparison to the controls, The PLD activities after 15, 30, 60, 120 and 240 min treatments of TRH (1μM) were 142%, 170%, 172%, 160% and 115%, respectively. Conclusion : These results suggest that TRH stimulates not only PLC activity, but also the PLD activity in GH_3 cells (J Kor Soc Endocrinol 17:465∼472, 2002).

訴訟遂行法

金仁燮,李東律 관동대학교 1996 關大論文集 Vol.24 No.2

Die Prozeβfuhrungsbefugnis ist die Befugnis, uber das streitige rechter oder Rechtseverhaltnis einen Rechtsstreit auf der Aktiv-oder Passiveseite im eigenen namen fuhren zu durfen. Die Prozeβfuhrungsbefugnis dient auch der Abwehr won Popularklagen, und ist genau von der Sachlegitimation zu unterschein, und es hangt eng mit dem formellem Parteibegriff zusammen. Partei sit, durch und gegen den rechtsschutz begehrt wird, eine materiellrechtliche Beziehung zum steitigen Rechisverhaltnis ist nicht erforderlich. Fehlt die Prozeβfuhrungsbefugnis, so wird die Klage als unzulassig abgewiesen, fehlt die Sachlegitimation, so ist die Klage als unbegrundet abzuwweisn. Die Prozeβfuhrungsbefugnis ist eine Proxeβvoraussetzung. Der Dritte hat das Recht, im eigenen Namen uber ein fremdes Recht oder eine fremde Verbindlichkeit zu prozessieren, in diesen Fallen spricht man von Prozeβstandschaft des Dritten. In diesen Prozeβstandschaft des Dritten sind eine gesetzlicher Prozeβstandschaft und eine gewillkurte Prozeβstandschaft.

Primary Productivity Measurement by Photosynthesis-Irradiance Model Method in Lake Soyang and the Behavior of Model Parameters

김범철,김동섭 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

광합성-광도 모델법을 이용하여 소양호의 일차 생산력 측정과 모델 변수의 동태를 조사하였다. 일차 생산력은 댐앞 지점에서 여름에 800∼1800mgC/㎥/day로 높고 겨울에 100mgC/㎥/dayd 이하로 낮게 나타났으며, Peridiniumbipes 가 밀집되는 계곡 유입부는 댐앞 지점보다 높게 나타났다. 광합성-광도 곡선의 초기 기울기(α), 광저해 계수(β) 그리고 Ik는 식품 플랑크톤의 적응 광도 변수와 신뢰성 있는 상관관계를 보여,α β 그리고 Ik가 광적옹도의 좋은 지표로 나타났다. 또한 동화 계수는 α β와 신뢰성 있는 양의 상관관계를 보였으며 이는 소양호에서 암적응된 식물플랑크톤이 α와 β가 크고 동화 계수도 크다는 것을 의미한다. α와 β는 채수수심의 광도보다 혼합층의 평균광도와 더 놓은 상관관계를 보여 식물 플랑크톤의 적응광도는 채수 수심의 광도가 아니고 혼합층의 평균 광도인 것으로 나타났다. Primary productivity was measured by phtosynthesis-irradiance (P-I) model method in a dendritic reservoir, Lake Soyang. Primary productivity was at the mesotrophicIeveI at the dam site. Productivity at the dam site was the highest in summer, 800-1800 mgC/m' / day, and low in winter, below 100. Primary productivity was higher in stream inlet regions where the migrating dinoflagellate, Peridinium lJipes, aggregates than the open water regions. P-I curves measured under the attenuated sun light or artificial light were fitted to a three parameter model. The initial slopes of P-I curves (a). the degree of photoinhiition (P), and Ik showed positive correlation with the light level that phytoplankton undergo. Therefore, α, β and Ik are good indicators of light-adaptation. AN showed positive correlation with α and β, which means that in Lake Soyang the shade-type pytoplankton has higher α and higher AN. The mean solar radiation of the mixing zone from surface to thermocline showed higher correlation with α and β than the solar radiation at the sampling depth. Therefore. it can be concluded that phytoplankton migrate through the mixing zone and phytoplankton is adapted to the mean light intensity of mixing zone, not the light intensity at the sampling depth.

Acetobactor xylinum endo-β-1,4-glucanase CMCax의 결정학적 연구

김동섭, 김준식, 정용제 忠北大學校部設基礎科學硏究所 2007 自然科學硏究 Vol.21 No.-

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