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The Gastrointestinal Metabolic Effects of Oat Product Based-β- glucan in Mice
Ji-Lin Dong,Wen-Li Zhang,Juan Lin,Rui-Ling Shen,Jun-Ling Si,Ying Wang 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.3
Most physiologicalstudies studies of oat beta(β)-glucan have shown positive effects on regulation ofblood glucose and lipid metabolism related to β-glucanmetabolism in the gastrointestinal tract. The effects of oatβ-glucan (OG), oat bran (OB), oat flour (OF), and oatmeal(OM) containing different levels of β-glucan on digestibilityand degradation were investigated in normal mice. Chymeviscosity, the gastric emptying rate, the gastrointestinaltransit rate, the activities of intestinal digestive enzymes,the levels of plasma cholecystokinin (CCK) and motlin(MOT), and degradation of β-glucan in oat products weredetermined. β-glucans from different oat products increasedintestinal chyme viscosity, delayed gastric emptying,promoted enterokinesia, decreased amylase, trypsin, lipase,and Na+, K+-ATPase activities, and promoted secretion ofCCK and MOT, especially for oat derived β-glucan.
Pathogenesis, Early Diagnosis, and Therapeutic Management of Alcoholic Liver Disease
Kong, Ling-Zu,Chandimali, Nisansala,Han, Ying-Hao,Lee, Dong-Ho,Kim, Ji-Su,Kim, Sun-Uk,Kim, Tae-Don,Jeong, Dong Kee,Sun, Hu-Nan,Lee, Dong Sun,Kwon, Taeho MDPI AG 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.11
<P>Alcoholic liver disease (ALD) refers to the damages to the liver and its functions due to alcohol overconsumption. It consists of fatty liver/steatosis, alcoholic hepatitis, steatohepatitis, chronic hepatitis with liver fibrosis or cirrhosis, and hepatocellular carcinoma. However, the mechanisms behind the pathogenesis of alcoholic liver disease are extremely complicated due to the involvement of immune cells, adipose tissues, and genetic diversity. Clinically, the diagnosis of ALD is not yet well developed. Therefore, the number of patients in advanced stages has increased due to the failure of proper early detection and treatment. At present, abstinence and nutritional therapy remain the conventional therapeutic interventions for ALD. Moreover, the therapies which target the TNF receptor superfamily, hormones, antioxidant signals, and MicroRNAs are used as treatments for ALD. In particular, mesenchymal stem cells (MSCs) are gaining attention as a potential therapeutic target of ALD. Therefore, in this review, we have summarized the current understandings of the pathogenesis and diagnosis of ALD. Moreover, we also discuss the various existing treatment strategies while focusing on promising therapeutic approaches for ALD.</P>
Cr(VI) Resistance and Removal by Indigenous Bacteria Isolated from Chromium-Contaminated Soil
( Dong Yan Long ),( Xian Jin Tang ),( Kuan Cai ),( Guang Cun Chen ),( Chao Feng Shen ),( Ji Yan Shi ),( Ling Gui Chen ),( Ying Xu Chen ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8
The removal of toxic Cr(VI) by microorganisms is a promising approach for Cr(VI) pollution remediation. In the present study, four indigenous bacteria, named LY1, LY2, LY6, and LY7, were isolated from Cr(VI)-contaminated soil. Among the four Cr(VI)-resistant isolates, strain LY6 displayed the highest Cr(VI)-removing ability, with 100 mg/l Cr(VI) being completely removed within 144 h. It could effectively remove Cr(VI) over a wide pH range from 5.5 to 9.5, with the optimal pH of 8.5. The amount of Cr(VI) removed increased with initial Cr(VI) concentration. Data from the time-course analysis of Cr(VI) removal by strain LY6 followed first-order kinetics. Based on the 16S rRNA gene sequence, strain LY6 was identified as Pseudochrobactrum asaccharolyticum, a species that had never been reported for Cr(VI) removal before. Transmission electron microscopy and energy dispersive X-ray spectroscopy analysis further confirmed that strain LY6 could accumulate chromium within the cell while conducting Cr(VI) removal. The results suggested that the indigenous bacterial strain LY6 would be a new candidate for potential application in Cr(VI) pollution bioremediation.
Ling Wang,Dong-yang Dai,Xia Wu,Yun-yan Sheng,Peng Ji,Dan-dan Li,Fan Zhang,Di Wang 한국원예학회 2021 원예과학기술지 Vol.39 No.5
The male sterile plants have higher heterosis in the production of hybrid seeds. The ABORTED MICROSPORES (AMS) gene has been demonstrated to be a candidate gene for ms-5. However, the genetic mechanism underlying AMS-mediated male sterility (MS) regulatory networks in melon (Cucumis melo L.) is still not clearly understood. In the present study, we used transcriptome sequencing analysis, yeast hybridization technology, quantitative real-time polymerase chain reaction (qRT-PCR), and bioinformatics analyzed to systematically investigate the AMS-mediated MS regulatory networks in melon. A set of 15 proteins interacting with AMS, including the C. melo L. Zinc Ribbon protein 1 (CmZR1) gene, was identified using the yeast one-hybrid (Y1H) system and further confirmed using the yeast two-hybrid (Y2H) assay. The interaction of the CmZR1 protein with the C. melo L. Pectin Methylesterase Inhibitor 1 (CmPMEI1) protein was identified and further verified by the glutathione S-transferase (GST) pull-down technique. Bioinformatics analyzed the physical and chemical properties, gene structure, and kinship of the melon PMEI family. We proposed a partial regulatory network for melon MS in which the interaction of CmPMEI1 protein with CmZR1 protein regulates the expression of the AMS gene for pollen abortion. These findings provide important information for increasing the understanding of the molecular mechanism of the MS regulatory network in melon.
Ji, Yu-Bin,Chen, Ning,Zhu, Hong-Wei,Ling, Na,Li, Wen-Lan,Song, Dong-Xue,Gao, Shi-Yong,Zhang, Wang-Cheng,Ma, Nan-Nan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21
Alkaloids are the most extensively featured compounds of natural anti-tumor herbs, which have attracted much attention in pharmaceutical research. In our previous studies, a mixture of major three alkaloid components (5, 6-dihydrobicolorine, 7-deoxy-trans-dihydronarciclasine, littoraline) from Hymenocallis littoralis were extracted, analyzed and designated as AHL. In this paper, AHL extracts were added to human liver hepatocellular cells HepG-2, human gastric cancer cell SGC-7901, human breast adenocarcinoma cell MCF-7 and human umbilical vein endothelial cell EVC-304, to screen one or more AHL-sensitive tumor cell. Among these cells, HepG-2 was the most sensitive to AHL treatment, a very low dose ($0.8{\mu}g/ml$) significantly inhibiting proliferation. The non-tumor cell EVC-304, however, was not apparently affected. Effect of AHL on HepG-2 cells was then explored. We found that the AHL could cause HepG-2 cycle arrest at G2/M checkpoint, induce apoptosis, and interrupt polymerization of microtubules. In addition, expression of two cell cycle-regulated proteins, CyclinB1 and CDK1, was up-regulated upon AHL treatment. Up-regulation of the Fas, Fas ligand, Caspase-8 and Caspase-3 was observed as well, which might imply roles for the Fas/FsaL signaling pathway in the AHL-induced apoptosis of HepG-2 cells.
Electrochemical preparation of Co<sub>3</sub>Pt nanowires
Min, Ji Hyun,Wu, Jun Hua,Cho, Ji Ung,Lee, Ju Hun,Ko, Young-Dong,Liu, Hong-Ling,Chung, Jin-Seok,Kim, Young Keun WILEY-VCH Verlag 2007 Physica status solidi. PSS. A, Applications and ma Vol.204 No.12
<P>Fabrication of Co-Pt nanowire arrays with fcc-Co<SUB>3</SUB>Pt phase was accomplished by DC electrodeposition in anodic aluminum oxide (AAO) nanotemplates. The microstructural and magnetic properties of the nanowires were investigated as a function of the deposition current density (Co concentration). The nanowires possess fcc-Co<SUB>3</SUB>Pt phases and are ferromagnetic. Their crystallinity and magnetic performance was enhanced after annealing. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)</P>
Multilocus Sequence Typing and Virulence Factors Analysis of Escherichia coli O157 Strains in China
Xiao W. Ji,Ya L. Liao,Ye F. Zhu,Hai G. Wang,Ling Gu,Jiang Gu,Chen Dong,Hong L. Ding,Xu H. Mao,Feng C. Zhu,Quan M. Zou 한국미생물학회 2010 The journal of microbiology Vol.48 No.6
Escherichia coli O157:H7, an important food-borne pathogen, has become a major public health concern worldwide. The aim of this study was to investigate the molecular epidemiologic feature of E. coli O157:H7strains in China. 105 E. coli O157:H7 isolates were collected from various hosts and places over 9 years. A multilocus sequence typing scheme (MLST) was applied for bacteria genotyping and polymerase chain reaction (PCR) was used for virulence factor identification. Seven new MLST sequence types (STs), namely ST836, ST837, ST838, ST839, ST840, ST841, and ST842 were identified, which grouped into two lineages. Phylogenetic analysis suggested that the most two frequent STs in China, ST837 and ST836, may be the derivatives of E. coli O157:H7 Sakai or E. coli O157:H7 EDL933. Geographical diversity and host variety of E. coli O157:H7 were observed in China. In addition, the different distribution of tccp was detected. The data presented herein provide new insights into the molecular epidemiologic feature of E. coli O157:H7, and aid in the investigation of the transmission regularity and evolutionary mechanism of E. coli O157:H7.