급성 구강감염부위에서 Staphylococcus lugunensis 의 특성

유용욱,이미성,차정단,김기경,신상희,문상은,김강주 원광대학교 생명공학연구소 1999 생명공학연구소보 Vol.6 No.1

Staphylococcus lugdunensis (S. lugdunensis) is a newly identified pathogenic species of coagulase negative staphylococci and an occasional but not rare cause of severe infections, such as infective endocarditis after dental extraction, bacteremia, osteomyelitis, peritonitis, and soft tissue infections. As antibiotic use increased, resistance rapidly developed. Some strains have plasmids related to antibiotic resistance. To characterize S. lugdunensis in acute oral infection, S. lugdunensis was isolated from the patients with acute oral abscess, osteomyelitis, and normal persons. Antibiotic resistance, in vitro cellular toxicity, in vivo virulence, δ-like hemolysin activity, and synergistic hemolysis on sheep blood agar plates were investigated. The dot blot analysis and Southern blot analysis of staphylococcal DNA was performed with δ-hemolysin gene probe of Staphylococcus aureus. (S. aureus) Staphylococcal DNA was cloned, nucleotide sequence was analysed, and homology was compared with other sequence in Gene Bank. S. aureus, S. lugdunensis, Staphylococcus cohnii (S. cohnii), and other coagulase negative staphylococci (CNS) were isolated from the patients with acute oral infection. The isolation ratio of S. lugdunensis in the patients with infection was higher than that of healthy persons, but the isolation ratio of S. aureus in the patients with infection was similar to that of healthy persons. S. lugdunensis from the patients with acute oral infection showed the resistance to penicillin, methicillin, cephalothin, and clindamycin. S. lugdunensis in the patients had cellular toxicity in vitro and virulence in vivo. All strains of S. lugdunensis had δ-like hemolysin activity against rabbit erythrocytes. Four of the six strains of S. lugdunensis gave synergistic hemolysis with S. aureus on sheep blood agar plates. In dot blot analysis, all strains of S. lugdunensis showed the positive reaction with the probe of δ-hemolysin gene in S. aureus, but a 7.3 kb HindⅢ fragment was observed in the DNA of S. lugdunensis that gave synergistic hemolysis in a Southern blot analysis. The molecular size of partially purified δ-hemolysin was about 50 kd. The cloned fragments from the chromosomal DNA of S. lugdunensis showed the partial homology with the insulin receptor-related and dopamine receptor of humans. These results suggest that S. lugdunensis might be an important pathogen in acute oral infection and show some homology with eukaryotes.

산국 (Chrysanthemum boreale) 휘발성 추출액 및 EDTA가 미생물 및 인체 치은 섬유아세포에 미치는 영향

차정단,김태영,우원홍,전병훈,김해경,유용욱,김강주,길봉섭 원광대학교 생체재료·매식연구소 2000 원광생체재료·매식 Vol.9 No.1

Chrysanthemum boreale (C. boreale) has been used for the treatment of furuncles, carbuncles, sores, conjunctivitis, headache, vertigo and swollen throats as a folk medicine in Korea, but there is little report about experiment evidence of antimicrobial activity. To investigate the antifungal and antibacterial activity of Chrysanthemum boreale(C. boreale) volatile substance and EDTA, the growth inhibition against Aspergillus nidulans (A. nidulans), Fusarium oxysporum (F. oxysporum), Alternaria mali(Al. mali), Candida albicans (C. albicans), staphylococcus aureus (S. aureus), Streptococcus pyogenes(S. pyogenes), streptococcus mutans (S. mutans), Escherichia coli(E. coli), Helicobacter pylori (H. pylori) and Porphyromonas gingivalis (P. gingivalis) was monitored by agar dilution and broth microdilution method. The antifungal effect of C. boreale on A. nidulans, F. oxysporum, Al. mali and C. albicans was shown at the concentration of 0.04%. The antibaterial effect of C. boreale on S. aureus, S. pyogenes, S. mutans and H. pylori was shown at the concentration of 0.04%. However, the growth was inhibited at the concentration of 0.04% irrespective of the presence of EDTA 0.125% was much higher than higher than that of several concentration of volatile substance. The minimum inhibitory concentrations of volatile substance were 0.16% against S. aureus, 0.04% against S. pyogenes, 0.02% against S. mutans, 0.40% against E. coli, and 0.18% against C. albicans. The growth of human gingival fibroblasts was not affected by C. boreale volatile substances at the concentration of 0.04%. These results indicate that volatile substance from C. boreale may have biologically toxic activities to the microorganisms.

Berberine의 항생제 내성 황색포도구균에 대한 항미생물 효과

김해경,차정단,유용욱,김강주,이동근 원광대학교 생체재료·매식연구소 2000 원광생체재료·매식 Vol.9 No.1

Antibiotic-resistant Staphylococcus aureus (S. aureus) emerged in many parts of the world and increased complex clinical problems. Therefore, new agents are needed to treat to antibiotic-resistant S. aureus. Some natural products are candidates of new antibotic substances. Previous reports showed that the extract of Coptidis Rhizoma and Phellodendri Cortex have antimicrobial effects. The main antibacterial substance of Coptidis Rhizoma and Phellodendri Cortex is berberine. In this study, we examined the antimicrobial activity of berberine to antibiotic-resistant S. aureus. We isolated the 13 strains of antibiotic-resistant S. aureus from the oral cavity of healthy children in a preschool, and investigated the antimicrobial activity of berberine. We examined the effects of berberine on the level of resistance to antibiotic-resistant S. aureus. We observed that the invasion of S. aureus into human gingival fibroblasts was blocked by berberine. Berberine inhibited the growth of antibiotic-resistant. S. aureus as a dose dependent maner. Minimum inhibition concentration (MIC) of berberine against antibiotic-resistant S. aureus was 50-100 ㎍/ml. The inhibition of S. aureus invasion was depended on the concentration of berberine in human gingival fibroblasts. The MIC of ampicillin against antibiotic-resistant S. aureus was 32 ㎍/ml. However, the MIC of ampicillin against antibiotic-resitant S. aureus in the presence of 50 ㎍/ml berberine was 1 ㎍/ml. These data indicate that the combination with berberine and β-lactam antibiotics resulted in the synergistic reaction. These results suggest that the berberine may have the antimicrobial activities to antibiotic-resistant. S. aureus, and the mechanism may be the inhibition of growth and intracellular invasion. and alteration of antibiotic-resistance level of antibiotic-resistant S. aureus.

Berberine의 항생제 내성 확색포도구균에 대한 항미생물 효과

김강주,차정단,김해경,유현희,유용욱,이동근 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.2

Antibiotic-resistant Staphylococcus aureus (S. aureus) emerged in many parts of the world and increased complex clinical problems. Therefore, new agents are needed to treat the antibiotic-resistant S. aureus. Some natural products are candidates of new antibiotic substances. Previous reports showed that the extract of Coptidis Rhizoma and Phellodendri Cortex have antimicrobial effects. The main antibacterial substance of Coptidis Rhizoma and Phellodendri Cortex is berberine. In this study, we examined the antimicrobial activity of berberine to antibiotic-resistant S. aureus. We isolated the 13 strains of antibiotic-resistant S. aureus from the oral cavity of healthy children in a preschool, and investigated the antimicrobial activity of berberine. We examined the effects of berberine on the level of resistance to antibiotic-resistant S. aureus. We observed that the invasion of S. aureus into human gingival fibroblasts was blocked by berberine. Berberine inhibited the growth of antibiotic-resistant S. aureus as a dosw dependent maner. Minimum inhibitory concentration (MIC) of berberine against antibiotic-resistant S. aureus was 50∼100㎍/㎖. The inhibition of S. aureus invasion was depended on the concentration of berberine in human gingival fibroblasts. The MIC of ampicillin against antibiotic-resistant S. aureus was 32㎍/㎖. However, the MIC of ampicillin against antibiotic-resistant S. aureus in the presence of 50㎍/㎖ berberine was 1㎍/㎖. These data indicate that the combination with berberine and β-lactam antibiotics resulted in the synergistic reaction. These results suggest that the berberine may have the antimicrobial activities to antibiotic-resistnat S. aureus, and the mechanism may be the inhibition of growth and intracellular invasion, and alteration of antibiotic-resistance level of antibiotic-resistant S. aureus.

Effect of Chrysanthemum boreale Essential Oil to several Microorganisms

(Joung Dan Cha),(Tae Yeong Kim),(Won Hong Woo),(Kyu Yong Chung),(Yong Ouk You),(Kang Ju Kim),(Bong Seop Kil) 원광대학교 생명공학연구소 2000 생명공학연구소보 Vol.7 No.1

N/A N/A

Haze-Free Highly Transparent Glass Substrates with Nanostructured Surface by Using Self-Assembled Ag Etch Masks

Zhao, Dan,Lee, Illhwan,Park, Jae Yong,Cho, Sang-Hwan,Choi, Chung Sock,Song, Seung-Yong,Kim, Jong Kyu,Lee, Jong-Lam The Electrochemical Society 2016 ECS journal of solid state science and technology Vol.5 No.2

<P>Nano- or micro-structured glass substrates have been widely used for displays, but have a significant drawback called haze that blurs the images. To eliminate haze while maintaining high total optical transmittance (T-total), the optimized design of textured structures on single- and double-side glass substrates was investigated using a rigorous coupled-wave analysis method. With optimized truncated cone-shaped nanostructures having period (Lambda) = 200 nm, top-diameter to bottom-diameter ratio (R) = 0.4 and height (h) >= 150 nm on both sides of glass substrates, we achieved a haze-free substrate with an average T-total = 99.5% in the wavelength (lambda) range 300-800 nm. (C) 2015 The Electrochemical Society. All rights reserved.</P>

Effects of PTTG Down-regulation on Proliferation and Metastasis of the SCL-1 Cutaneous Squamous Cell Carcinoma Cell Line

Xia, Yong-Hua,Li, Min,Fu, Dan-Dan,Xu, Su-Ling,Li, Zhan-Guo,Liu, Dong,Tian, Zhong-Wei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Aims: To study effects of down-regulation of pituitary tumor-transforming gene (PTTG) on proliferation and metastasis ability of the SCL-1 cutaneous squamous cell carcinoma (CSCC) cell line and explore related mechanisms. Methods: SCL-1 cells were divided into 3 groups (untreated, siRNA control and PTTG siRNA). Cell proliferation assays were performed using a CCK-8 kit and proliferation and metastasis ability were analyzed using Boyden chambers. In addition, expression of MMP-2 and MMP-9 was detected by r-time qPCR and Western blotting. Results: Down-regulation of PTTG could markedly inhibit cell proliferation in SCL-1 cells, compared to untreated and control siRNA groups (P < 0.05). Real-time qPCR demonstrated that expression levels of PTTG, MMP-2 and MMP-9 in the PTTG siRNA group were 0.8%, 23.2% and 21.3% of untreated levels. Western blotting revealed that expression of PTTG, MMP-2 and MMP-9 proteins in the PTTG siRNA group was obviously down-regulated. The numbers of migrating cells ($51.38{\pm}4.71$) in the PTTG siRNA group was obviously lower than that in untreated group ($131.33{\pm}6.12$) and the control siRNA group ($127.72{\pm}5.20$) (P < 0.05), suggesting that decrease of proliferation and metastasis ability mediated by PTTG knock-down may be closely correlated with down-regulation of MMP-2 and MMP-9 expression. Conclusion: Inhibition of PTTG expression may be a new target for therapy of CSCC.

Outbound Open Innovation for Enhanced Innovation Performance : An Empirical Study based on BTB Enterprises in China

Huan-Yong Ji,Jing Li,Chong-Feng Lan,Dan-Feng, Wang,Yong-Sheng Jin 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.5

Open Innovation gains a wide spread attention in technology management research in recent years. Because existing research about the relationship between outbound open innovation and innovation performance is limited and context factors are ignored in existing research and based on Chesbrough’s open innovation model and theory of enterprise competence, this study introduces enterprise competence and knowledge spillovers into the field of outbound open innovation, explores their moderated roles between open innovation and innovation performance. We use high-tech firms as research sample to test the hypothesis that we proposed and find that: (1) outbound open innovation has a positive effect on innovation performance; (2) both enterprise competence and knowledge spillovers moderate the relationship between outbound open innovation and innovation performance. These conclusions have important implications for the development and application of open innovation theory.

Effect of Lowering Laser Energy on the Surface Roughness of Human Corneal Lenticules in SMILE

Ji, Yong Woo,Kim, Minseo,Kang, David Sung Yong,Reinstein, Dan Z.,Archer, Timothy J.,Choi, Jin Young,Kim, Eung Kweon,Lee, Hyung Keun,Seo, Kyoung Yul,Kim, Tae-im SLACK INCORPORATED 2017 Journal of refractive surgery Vol.33 No.9

Antimicrobial Effect of Volatile Substances of Chrysanthemum boreale and its Gas Chromatographic Analysis

Joung Dan Cha,Bong Seop Kil,Yong Ouk You,Yong lk Kim,Sang Gu Hwang,Byung Hun Jeon,Kang Ju Kim 한의병리학회 2001 동의생리병리학회지 Vol.15 No.5