복부기형혈관의 문맥-체순환 단락으로 유발된 만성 후천성 간뇌 퇴행중 1례

김대현,김종국,차재관,김상호,김재우 대한신경과학회 1998 대한신경과학회지 Vol.16 No.2

체성감각 유발전위중 Nl8 Far-field 전위의 비강인두를 통한 접근

김대현,차재관,김상호,김재우 대한신경과학회 1999 대한신경과학회지 Vol.17 No.1

소뇌뇌교각종양에서 수술중 전기생리학적 신경계집중감시의 변화

서대원,박관,안재영,이상구,정진상,홍승봉,이원용,김병준,김종현 대한신경과학회 1999 대한신경과학회지 Vol.17 No.1

cDNA array 방법을 이용한 망간에 노출된 흰쥐 뇌기저핵의 유전자발현 분석

이채관,노성민,문덕환,,김정호,손병철,김대환,이창희,김휘동,김정원,김종은,안진홍,이채언 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Objectives: This study investigated the gene expression profile in basal ganglia of manganese-exposed rats based on cDNA array analysis. Methods: For cDNA array, 25 male Sprague-Dawley rats (250±25 g) were intraperitoneally injected with 25 ㎎/㎏ B. W./day of MnCl2 (0.3 ㎖) for 10 days. For dose-related gene expression analysis, rats were intraperitoneally injected with 0.2, 1.0, and 5.0 ㎎/㎏ B. W/day of MnCl2 for 10 days. Control rats were injected with an equal volume of saline. RNA samples were extracted from brain tissue and reverse-transcribed in the presence of [α^(32)P]-dATP. Membrane sets of the Atlas Rat 1.2 array Ⅱ and Toxicology array 1.2 kit (Clontech, Palo Alto, CA) were hybridized with cDNA probe sets. Northern blot hybridization method was employed to assess the dose-related gene expression. Results: Fifty-two genes showed significant changes in expression of more than two-fold. Twenty-eight were up-regulated and 24 were down-regulated in the manganese-exposed group compared to the control. Among the 52 genes, 28 genes including nuclear factor I-X1 (NF1-X1), neuroligin 2 and 3, mitochondrial stress-70 protein (MTHSP70), neurodegeneration-associated protein 1 (Neurodap 1), multidrug resistance protein (MDR), and endoplasmic reticulum stress protein 72 (ERP72), were reported for the first time related to the manganese-induced neurotoxic-metabolism in the rat basal ganglia. According to the dose-related gene expression analyses, MTHSP70, Neurodap 1 and ERP72 genes were up-regulated compared to the control even in the group exposed to low manganese dose ( 0.2 ㎎/㎏ B.W./day). Conclusions: Twenty-eight genes detected for the first time in this study were closely related to the manganese-induced neurotoxic-metabolism in the rat basal ganglia and further study of these genes can give some more useful information about the manganese metabolism.

국어과 교육과정 언어 영역의 지도 방향

기세관,이승대 順天大學校 師範大學 附屬 科學敎育硏究所 1997 科學과 敎育 Vol.5 No.-

This study aims to discuss the following devices of teaching; Knowledge covered in the whole realm of language should be taught. At the same time, teachers should keep the following in mind, how to transfer knowledge that can be used in real situations, how they can draw functional elements and potential value(cultural element) from it, and how to make students understand to the highest degree. Teaching language should be achieved by increasing the spirit of inquiry, and the power of thought and enhancing student centered learning - that is, putting more weight or value on student activity rather than teacher centered learning. An appropriate model of teaching would include a step by step plan like the following: setting a goal →inquisitive learning →development(improvement) and understanding →assessment. This is a model that allows us to realize the importance of cultural value in the inquisitive process and the realm of language education. To be successful, it is necessary for teachers to have various materials, appropriate questioning, and a democratic and permissive attitude.

Mossbauer 分光法과 選擇的 抽出方法에 의한 濟州島 土壤에서의 酸化鐵의 特性 究明

宋寬哲,姜東佑,金斗哲,高正大,洪性樂 濟州大學校 基礎科學硏究所 1997 基礎科學硏究 Vol.10 No.1

濟州島의 8개 代表土壤을 對象으로 하여 X-線 螢光分析, X-線 回折 分析, oxalate와 dithionite-citrate를 사용한 選擇的 抽出方法 및 Mossbauer 分光法으로 一次鑛物, ferrihydrite, 結晶質 酸化鐵의 種類, 含量 및 非磁性 Al이온의 置煥程度에 대하여 考察하였다. 1. X-線 螢光 分析에 의한 濟州島 8개 土壤統의 酸化鐵(Fe₂O₃) 含量은 噴石口 토양인 松岳統에서 18.21%로 가장 높았고 논토양인 大靜統에서 8.03%로 가장 낮았다. 2. X-線 回折 分析 結果 8개 土壤統 모두에서 ferrihydrite와 結晶質 goethite의 回折 피크는 나타나지 않았지만, 結晶質 hematite는 松岳統, 舊巖統 및 黑岳統에서 檢出할 수 있었다. 3. 선택적 침출방법으로 측정한 ferrihydrite 含量은 흑색토인 松堂과 吐坪統, 농암갈색의 분석구 토양인 柑山統 및 갈색삼림토인 黑岳統에서 20.9-35.2g/㎏으로 높았고, 논토양인 大靜統에서 0.85g/㎏으로 가장 낮았다. 結晶質 酸化鐵 含量인 Fed-Feo 含量 松岳統에서 28.7g/㎏으로 가장 높았고 黑岳統에서 7.6g/㎏으로 가장 낮았다. 4. 濟州島 8개 土壤統에 함유되어 있는 常磁性 Fe³+ 鑛物들은 化學造成이 거의 같은 ferrihydrite, 超常磁性 goethite, 층형 硅酸鑒粘土鑛物로서 柑山統에서 그 含量이 가장 높았고 松岳統에서 가장 낮은 것으로 분석되었다. 常磁性 Fe²+ 一次鑛物은 柑山統과 黑岳統에서 olivine, illite의 含量이 優勢하였으나 그 외 土壤에서는 小量의 chlorite, augite, biotite, hornblende 등이 含有되어 있는 것으로 분석되었다. 5. 18K에서 측정된 超微細磁氣場값으로 부터 大靜統에서는 미세한 goethite 만이 含有되어 있는 것으로 確認되었고, 그외 7개 土壤에서는 입자 크기가 작은(∼180Å) 結晶質 hematite와 goethite이 것으로 分析되었다. 6. 濟州島 8개 土壤統에 함유되어 있는 酸化鐵의 구조에서 Fe³+와 非磁性 Al³+이 相堂量 部分 置煥되었음을 確認할 수 있었다. Iron oxide compounds in 8 selected Cheju Island soil samples have been analized by X-ray fluorescence spectrometer(XRF), X-ray diffractometry(XRD), selected chemical techniques, and Mossbauer spectroscopy. The result of this analysis by XRF shows that the rate of quantity of Fe₂O₃ in 8 soil samples was from 8.03wt.%(Daejeong paddy soil ) to 18.21wt.%(Songag soils). Songag, Heugag and Gueom soils were detected to have lower peaks of intensity of hematite by XRD. In addition, these soils were not detected to have hematite and goethite peaks. Ferrihydrite, which is a short-range-order mineral commonly present in volcanic ash soil, was not detected by XRD due to low concentration and/or poor cristallinity. Ferrihydrite contents estimated from Feo values were 8.8∼35.2g/㎏ for volcanic ash soils and 0.85g/㎏ for the Daejeong soil. Most of the soil samples represented by the paramagnetic Fe³+ doublet obtained from Mossbauer spectra at room temperature and 18K were considered to arise from the presence of ferrihydrite, superparamagnetic goethite, and silicate minerals. Also the paramagnetic Fe²+ doublets are attributable to primary minerals such as olivine, illite, chlorite, augite, biotite, and hornblende. Goethite and hematite were identified as the dominant crystalline iron oxides in these soils from Mossbauer spectra obtained at room temperature and 18K. All the soil samples exhibited strong superparamagnetic relaxation. Collapse of the Mossbauer magnetic hyperfine splitting at room temperature was due to the small size(∼180Å) of the oxide particles and/or Al-subsituted goethite.

Toluene, Xylene, Trichloroethylene 투여가 흰쥐의 시상하부와 뇌하수체의 GnRH, GnRH Receptor, Pit-1 유전자 발현에 미치는 영향

기노석,이종태,강대성,김대환,이채관,이헌,전진호,김정호,이채언 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.2

The workplace exposure of chemicals has steadily increased, therefore the concern for subsequent effect on reproductive outcome has been an important issue in occupational medicine. In previous studies, higher rates of spontaneous abortion, reduced fertility and menstrual disorder among women, and an impairement of sperm quantity and quality among men have been associated with a wide variety of chemical agents. This study was conducted to evaluate the effects of toluene, xylene and trichloroethylene(TCE) injection on the mRNA levels of GnRH, GnRH receptor and Pit-1 genes in male rats hypothalamus and pituitary and the effects on the plasma levels of FSH, LH, prolactin and testosterone. Sprague-Dawley male rats were divided into five groups of five each according to concentration of toluene, xylene and TCE. The rats were injected subcutaneously to 0, 50, 100, 200, 400 mg/kg body weight/day of toluene, xylene and TCE, respectively for 6 days. Rat brains were excised and hypothalamus and pituitary were separated. Reverse transcription-polymerase chain reaction (RT-PCR) and RNase protection assay(RPA) were used to evaluate the GnRH, GnRH receptor and Pit-1 mRNA levels. Plasma concentrations of FSH, LH, prolactin and testosterone were assayed by radioimmunoassay(RIA). The results were as follows; 1. GnRH, GnRH receptor and Pit-1 mRNA levels in toluene and xylene injected groups, and GnRH receptor mRNA levels in TCE injected group were lowered dose-dependently. Especially, GnRH receptor and Pit-1 mRNA levels in 200mg/kg of toluene injected group, and GnRH, GnRH receptor and Pit-1 mRNA levels in 400mg/kg of toluene injected group were significantly lower than control group (p<0.05). GnRH receptor and Pit-1 mRNA levels in 400mg/kg of xylene injected group, and GnRH receptor mRNA levels in 400mg/kg of TCE injected group were significantly lower than control group(p<0.05). 2. The plasma levels of prolactin and testosterone in 400mg/kg of toluene injected group, and LH in 100, 200 and 400mg/kg of xylene injected group, and testosterone in 400 mg/kg of TCE injected group were significantly lower than control group(p<0.05). In conclusion, we speculated that toluene and xylene affected reproductive system secondarily through hypothalamus-pituitary axis, and TCE affected directly through steroidogenesis. And we recommended that further study for assessment of the reproductive toxicity of mixed organic solvent exposures should be conducted.

소규모사업장 용접근로자들의 소음·용접흄·망간 노출에 관한 연구

김동구,문덕환,이채관,박명희,함성애,손병철,김대환,이창희,김휘동,이채언 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.5

Objective : This study was conducted for the purpose of investigating the level exposure of welders to noise, welding fume and Manganese(Mn) related to this process. Methods and Material : All subjects in this study were welders engaged in CO2 arc and coated arc welding process especially in small-scale enterprises classified by type of manufacture of ship building, repairing and of other fabricated metal products, which is located in Gimhae and Milyang of Kyeongsang province in south Korea. Air sampling of welding fume was measured, shipped and analyzed on the basis of national institute for occupational safety and health(NIOSH) method in U.S. and work environment measurement method established in south Korea. To measure Noise in the work environment, Noise dosimeter was used on the basis of work environment measurement method established in south Korea. Result : This study was performed during a period from January, 2000 to December, 2001 and Results were as follows: 1. Noise level measured in this work environment was higher than national criteria for permissible exposure limit on health examinations related to hearing loss. 2. The concentration of welding fume and Mn in manufacture of ship building and repairing was higher than those in manufacture of other fabricated metal products. 3. In conditions of existing in ventilation system installed and operated, the concentration of welding fume and Mn was slightly lower than those of not existing. 4. In conditions of existing in sufficient air supply from out door into the work place relied on natural exhaust system, the concentration of welding fume and Mn was slightly lower than those of in not sufficient. 5. In all subjects, a rate of wearing ear plug and respiratory mask for dust and fume was lower than that of not wearing. Conclusion : According to above results, this study suggests that more positive work environment managements such as the use of portable local ventilation system, the supply of highly qualified personal protective equipment, and consecutive education and training related to health protection should be need to acquire suitable health protection program for welders engaged in small-scale enterprises in Korea.

복직근 및 박근 근피판술을 이용한 골반 및 서혜부 연부조직 결손의 재건

이재화,정윤규,김주봉,유대현,황성관,이훈범,김석원 大韓成形外科學會 1997 Archives of Plastic Surgery Vol.24 No.6

Many operations for the reconstruction of the pelvic area have been studied until now. The local muscle flap is often used for the closure of these complex wound. But, these local muscle flaps may not be available for reconstruction due to the factors of infection, radiation, and surgical trauma. Since rectus abdominis muscle and gracilis muscle were introduced, their use has been steadily increased for the reconstruction of soft tissue defect of the pelvic area. Inferiorly based rectus abdominis muscle flap and the gracilis muscle flap have been adopted for well-vascularized soft tissue coverage of the defect of pelvis and groin area. These techniques were utilized in seven patients. Of these patients, rectus abdominis muscle flap was used on four patients and three patients were treated with gracilis muscle flap. Among these, chronic osteomyelitis of pelvic bone has been managed with inferiorly based rectus abdominis muscle flap in three patients. No complication has been found. The mean postoperative follow-up period was 26 months, with a range of 12 to 39 months. In conclusion, these muscles are functionally expandable , and provide adequate tissue volume with sufficient blood supply away from the zone of injury, and readily transposed into the pelvis and groin area through subcutaneous tunnel, and serve as an antibiotic delivery system that aids in the eradication of infection. These findings suggest a reconstructive option for the defect of pelvis and groin, and/or bone infection in this area.

접는 미사일 조종날개의 비선형 힌지 동특성 파악

김대관(Dae-Kwan Kim),배재성(Jae-Sung Bae),이인(In Lee),우성현(Sung-Hyun Woo) 한국소음진동공학회 2002 한국소음진동공학회 학술대회논문집 Vol.2002 No.5