화옹호의 부영양화 방지를 위한 영양염류 삭감률 산정

김미아,김영희,이홍근,황대호,김지영 한국물환경학회 2004 한국물환경학회지 Vol.20 No.6

In this study, the reduction rates of nutrients were suggested to prevent eutrophication on the Hwaong reservoir in the year of 2008 and 2012. With EPA's WASP6 model, future water quality were simulated. In 2008, T-N would be 1.36mg/L and T-P 0.100mg/L on average. ; In 2012, T-N 2.66mg/L and T-P 0.128mg/L. With all the water quality management plans that the government authorities are carrying out, these results indicate that the reservoir would be reach the eutrophic or hypertrophic state according to the Vollenweider's trophic states. Therefore, the Hwaong reservoir requires additional plans for nutrients management. Here, the target water quality to prevent eutrophication of the reservoir sets into mesotrophic state ; T-N 0.475mg/L and T-P 0.02mg/L.(median of Vollenweider index for mesotropphic state) The reduction rates of nutrients on Namyang and Eoeun streams were estimated with uniform treatment method to meet the goal. The results showed that nutrients from two streams should be reduced up to 78% in 2008, and 84% in 2012. Since the ratio of T-N/T-P would be higher than 16 at target years, T-N was not considered as the limiting factor and was not reduced.

Early Experience of Laparoscopic Management for Choledochal Cysts

Dae Wook Hwang,Jae Hoon Lee,Sang Yeup Lee,Dae Keun Song,Kwang-Min Park,Young-Joo Lee 한국간담췌외과학회 2010 한국간담췌외과학회 학술대회지 Vol.2010 No.4

고랭지 사계성 딸기 품종 특성 비교 및 ‘Charlotte’ 품종의 예냉 처리 효과

황대근(Dae Keun Hwang),엄향란(Hyang Lan Eum),용영록(Young Rog Yeoung),박권우(Kuen Woo Park),홍세진(Sae Jin Hong) 한국원예학회 2013 원예과학기술지 Vol.31 No.3

본 연구는 해발 780m 이상의 고랭지에서 재배된 사계성 딸기의 8개 품종에 대해서 품종 별 특성조사를 통해 이용목적에 따라 분류를 하고, 주요 수출품종인 ‘Charlotte’의 저장 및 유통 중 고품질 유지를 위해 전처리 기술 중 통풍식 예냉처리를 실시하여 품질변화를 조사하였다. 가공용으로 이용되는 사계성 딸기의 품종별 경도는 1N 이상과 당도는 6°Brix 이상의 값이 요구되며 ‘Charlotte’ 품종이 1.5N 이상으로 가장 경도가 높았다. 가용성 고형물 함량은 ‘Portola’와 ‘San Andreas’ 품종을 제외한 6 종류 품종 모두 6°Brix 이상의 값을 나타냈으며 ‘Albion’, ‘Charlotte’, 그리고 ‘Goha’ 품종이 다른 품종에 비해 유의적 차이를 보이며 높게 나타났다. 색도 지수인 hue angle과 chroma 값은 ‘Charlotte’, ‘Flamenco’, ‘GW-4’, 그리고 ‘San Andreas’ 품종에서는 높으며 좀더 선명한 붉은색을 띄었다. 저온 저장실험에 이용한 ‘Charlotte’ 품종은 상온에 저장할 경우 저장 3일이 경과되면서 8% 이상의 중량 감모율을 보였다. 반면 4℃와 10℃의 저온저장은 저장 7일 동안 2%의 중량 감모율을 보였다. 저온저장 중에는 온도에 따른 유의적 차이는 없었으나 shelf-life 기간 동안에는 9%까지 무게손실이 증가되었으며 통풍식예냉 처리가 무예냉 처리에 비해서 중량 감모율이 적었다. 가용성 고형물의 함량은 모든 처리구에서 6-8°Brix를 유지하는데, 예냉처리에 따른 유의적 차이는 없었다. 곰팡이 발생은 저온 4℃ 저장에서 예냉 유무에 관계없이 발생되지 않았으나 shelf-life 기간 동안에는 3일 경과 후부터 발생되었다. 과육의 색도는 저장기간 동안에는 온도처리 및 예냉 처리 유무에 따라서 일정한 경향이 없이 증감을 보이다가 상온으로 옮긴 shelf-life 기간 동안에는 4℃ 저장된 과실의 경우 착색이 지연되었다. 수출 시 고랭지 사계성 딸기의 품질을 유지하기 위해서는 수확 후 4℃ 이하의 온도조건을 유지하는 것이 중요하며 통풍식 예냉 효과는 미비하였다. This study was conducted to investigate the quality characteristics of eight everbearing strawberry cultivars and the effect of precooling treatment to maintain the quality during storage and shelf life of ‘Charlotte’. Several quality parameters including firmness, soluble solids content (SSC), color, soluble sugars, and organic acids were evaluated. For Successful marketing everbearing strawberries required more than 1 N of firmness and 6 °Brix of SSC, respectively. ‘Albion’, ‘Charlotte’, and ‘Goha’ cultivars were higher in fruit SSC and ‘Charlotte’ cultivar was higher in fruit firmness among eight cultivars examined in this study. Fruit had more bright red color in ‘Charlotte’, ‘Flamingo’, ‘GW-4’, and ‘San Andreas’, which may reflect the consumer acceptance. Physic℃hemical characteristics of ‘Charlotte’ strawberry after with/without room precooling treatment were evaluated during storage at 4℃, 10℃, and 25℃ and additional 3 days at 25℃ for shelf-life. Weight loss increased about 8% in the fruits stored at 25℃ for 3 days, while in low temperature storage was about 2% for 7 days showing precooling was more effect on during shelf life periods rather than storage periods. Decay was not found during storage at 4℃ regardless of precooling treatment, but fungal growth was found in all the treatments after transferred to room temperature. Precooling treatment did not affect fruit color in shelf life period. For advanced marketing, everbearing strawberry should maintain in low temperature less than 4℃ to sustain quality, but the room precooling showed less significant effect to maintain quality on ‘Charlotte’ cultivar.

바실 수경재배시 셀레늄 처리를 통한 저장 중 저온장해 완화 효과

황대근(Dae Keun Hwang),이문정(Moon Jung Lee),강호민(Ho Min Kang),박권우(Kuen Woo Park) 한국원예학회 2000 원예과학기술지 Vol.18 No.5

한랭지 반촉성재배시 정식시기가 딸기의 생육 및 수량에 미치는 영향

황대근(Dae Keun Hwang),박권우(Kuen Woo Park),용영록(Young Rog Yeoung) 한국원예학회 2011 한국원예학회 학술발표요지 Vol.2011 No.5

Production of Cloned Korean Native Pig by Somatic Cell Nuclear Transfer

In-Sul Hwang,Dae-Jin Kwon,Keun Bong Oh,Sun-A Ock,Hak-Jae Chung,In-Cheol Cho,Jeong-Woong Lee,Gi-Sun Im,Seongsoo Hwang 한국발생생물학회 2015 발생과 생식 Vol.19 No.2

The Korean native pig (KNP) have been considered as animal models for animal biotechnology research because of their relatively small body size and their presumably highly inbred status due to the closed breeding program. However, little is reported about the use of KNP for animal biotechnology researches. This study was performed to establish the somatic cell nuclear transfer (SCNT) protocol for the production of swine leukocyte antigens (SLA) homotype-defined SCNT KNP. The ear fibroblast cells originated from KNP were cultured and used as donor cell. After thawing, the donor cells were cultured for 1 hour with 15 μM roscovitine prior to the nuclear transfer. The numbers of reconstructed and parthenogenetic embryos transferred were 98 ± 35.2 and 145 ± 11.2, respectively. The pregnancy and delivery rate were 3/5 (60%) and 2/5 (40%). One healthy SLA homotype-defined SCNT KNP was successfully generated. The recipient-based individual cloning efficiency ranged from 0.65 to 1.08%. Taken together, it can be postulated that the methodological establishment of the production of SLA homotype-defined cloned KNP can be applied to the generation of transgenic cloned KNP as model animals for human disease and xenotransplantation researches.

수평자기와 수직자기 구조에서 Laser Annealing effect

최상대(Sang-Dae Choi),진대현(Dae-Hyun Jin),김선욱(Sun-Wook Kim),윤대근(Dae-Keun Yun),안진희(Jin-Hee An),이미선(Mee-Sen Lee),주호완(Ho-Wan Joo),이기암(Ky-Am Lee),이상석(Sang-Suk Lee),황도근(Do-Geun Hwang) 한국자기학회 2004 한국자기학회 학술연구발표회 논문개요집 Vol.14 No.2

Transgenic Efficiency of FoxN1-targeted Pig Parthenogenetic Embryos

Jae-Hoon Yeo,In-Sul Hwang,Jae Kyung Park,Dae-Jin Kwon,Seoki Im,Eung-Woo Park,Jeong-Woong Lee,Choon-Keun Park,Seongsoo Hwang 한국수정란이식학회 2014 한국동물생명공학회지 Vol.29 No.4

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas9) system can be applied to produce transgenic pigs. Therefore, we applied CRISPR/Cas9 system to generate FoxN1-targeted pig parthenogenetic embryos. Using single guided RNA targeted to pig FoxN1 genes was injected into cytoplasm of in vitro matured oocyte before electrical activation. In results, regardless of the concentrations of vector, the cleavage rate were significantly (p<0.05) decreased (4 ng/μl, 51.24%; 8 ng/μl, 40.88%; and 16 ng/μl; 45.22%) compared to no injection group (70.44%). The blastocyst formation rates were also decreased in vector injected 3 groups (4 ng/μl, 7.96%; 8 ng/μl, 6.4%; and 16 ng/μl; 9.04%) compared to no injection group (29.07%). In addition, the blastocyst formation rates between sham injected group (13.51%) and no injection group (29.07%) also showed significant difference (p<0.05). The mutation rates were comparable between groups (4 ng/μl, 18.4%; 8 ng/μl, 12.5%; and 16 ng/μl; 20.0%). The sequencing analysis showed that blastocysts derived from each group were successfully mutated in FoxN1 loci regardless of the vector concentrations. However, the deletion patterns were higher than the patterns of point mutation and insertion regardless of the vector concentrations. In conclusion, we described that cytoplasmic microinjection of FoxN1-targeted CRISPR/Cas9 vector could efficiently generate transgenic pig parthenogenetic embryos in one-step.

소아 아나필락시스양 자반증에 대한 임상적 고찰

황대성,김병현,김연심,오광수,오연균,윤향석,김종덕 圓光大學校 醫科學硏究所 1991 圓光醫科學 Vol.7 No.1-2

The authors experienced 29 cases of Henoch-Schoenlein purpuras during the period from April 1986 to April 1991. The summaries of clinical and laboratory observations are as follows. 1) Male to female ratio was 2.6 : 1 and the age of peak incidence was among 4 to 7 years of age. 2) In seasonal incidence, the case was most frequent during spring(45 %}. 3) The previous history of abdominal pain was noted in 11 cases(65 % ) and 6 cases(35 %) showed infection of the upper respiratory tract. 4) The main clinical manifestations were skin rash(100 %), abdominal symptoms(83 %), joint manifestations(62 %), and renal involvement(24 %). 5) The most commonly observed manifestations(23 cases, 80 %) of the skin were petechiae, purpuras, and ecchymosis. 6) The abdominal pain was the most common manifestation of gastrointestinal symptoms (23 cases, 80 %). 7) The knee was the most commonly involved joint(59 %). 8) The renal involvement was observed in 7 cases(24 %), and the main manifestations of renal involvements were microscopic hematuria and proteinuria. 9) Leukocytosis over 10,000/㎣ was was showed in 62 % of cases, and ESR over 20 ㎜/hr was in 28 % of cases but blood clotting studies were mostly within normal ranges. 10) ASO titer over 166 Todd units was observed in 35 % of cases and C-reactive protein was positive in 35 % of cases. 11) Most clinical manifestations subsided within 4 weeks.

Production of Cloned Korean Native Pig by Somatic Cell Nuclear Transfer

Hwang, In-Sul,Kwon, Dae-Jin,Oh, Keun Bong,Ock, Sun-A,Chung, Hak-Jae,Cho, In-Cheol,Lee, Jeong-Woong,Im, Gi-Sun,Hwang, Seongsoo The Korean Society of Developmental Biology 2015 발생과 생식 Vol.19 No.2

The Korean native pig (KNP) have been considered as animal models for animal biotechnology research because of their relatively small body size and their presumably highly inbred status due to the closed breeding program. However, little is reported about the use of KNP for animal biotechnology researches. This study was performed to establish the somatic cell nuclear transfer (SCNT) protocol for the production of swine leukocyte antigens (SLA) homotype-defined SCNT KNP. The ear fibroblast cells originated from KNP were cultured and used as donor cell. After thawing, the donor cells were cultured for 1 hour with 15 ${\mu}M$ roscovitine prior to the nuclear transfer. The numbers of reconstructed and parthenogenetic embryos transferred were $98{\pm}35.2$ and $145{\pm}11.2$, respectively. The pregnancy and delivery rate were 3/5 (60%) and 2/5 (40%). One healthy SLA homotype-defined SCNT KNP was successfully generated. The recipient-based individual cloning efficiency ranged from 0.65 to 1.08%. Taken together, it can be postulated that the methodological establishment of the production of SLA homotype-defined cloned KNP can be applied to the generation of transgenic cloned KNP as model animals for human disease and xenotransplantation researches.