Protein Profile in Corpus Luteum during Pregnancy in Korean Native Cows

Chung, H.J.,Kim, K.W.,Han, D.W.,Lee, H.C.,Yang, B.C.,Chung, H.K.,Shim, M.R.,Choi, M.S.,Jo, E.B.,Jo, Y.M.,Oh, M.Y.,Jo, S.J.,Hong, S.K.,Park, J.K.,Chang, W.K. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.11

Steroidogenesis requires coordination of the anabolic and catabolic pathways of lipid metabolism, but the profile of proteins associated with progesterone synthesis in cyclic and pregnant corpus luteum (CL) is not well-known in cattle. In Experiment 1, plasma progesterone level was monitored in cyclic cows (n = 5) and pregnant cows (n = 6; until d-90). A significant decline in the plasma progesterone level occurred at d-19 of cyclic cows. Progesterone level in abbatoir-derived luteal tissues was also determined at d 1 to 5, 6 to 13 and 14 to 20 of cyclic cows, and d-60 and -90 of pregnant cows (n = 5 each). Progesterone level in d-60 CL was not different from those in d 6 to 13 CL and d-90 CL, although the difference between d 6 to 13 and d-90 was significant. In Experiment 2, protein expression pattern in CL at d-90 (n = 4) was compared with that in CL of cyclic cows at d 6 to 13 (n = 5). Significant changes in the level of protein expression were detected in 32 protein spots by two-dimensional polyacrylamide gel electrophoresis (2-DE), and 23 of them were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Six proteins were found only in pregnant CL, while the other 17 proteins were found only in cyclic CL. Among the above 6 proteins, vimentin which is involved in the regulation of post-implantation development was included. Thus, the protein expression pattern in CL was disorientated from cyclic luteal phase to mid pregnancy, and alterations in specific CL protein expression may contribute to the maintenance of pregnancy in Korean native cows.

HWE(Hot Wall Epitaxy)에 의한 ZnIn_2S_4 박막 성장과 광전도 특성

홍광준,이관교,정준우,정경아,방진주,장현규,문종대,김혜숙 조선대학교 기초과학연구소 1999 自然科學硏究 Vol.22 No.1

HWE 방법에 의해 ZnIn_2S_4 박막을 Si(00) 기판 위에 성장시켰다. 증발원과 기판의 온도를 각각 610℃, 450℃로 하여 성장시킨 ZnIn_2S_4 박막의 이중 결정 X-선 요동곡선(DCRC)의 반폭치(FWHM)값이 245 arcsec로 가장 작았다. Van der Pauw 방법으로 Hall 효과를 측정하여 운반자농도의 1n n 대 1/T에서 구한 활성화에너지는 0.17eV로 측정되었다. Hall 이동도의 온도 의존성은 30K에서 100K까지는 불순물산란에 기인하고, 100K에서 293K까지는 격자산란에 기인한것으로 고찰되었다. 광전도셀의 특성으로 spectral response, 최대 허용소비전력(MAPD), 광전류와 암전류(pc/dc)의 비 및 응답시간을 측정하였다. S 증기분위기에서 열처리한 광전도 셀의 경우, 감도(??)는 0.99, pc/dc은 1.37x10^7, 그리고 최대 허용소비전력(MAPD)은 336mW, 오름시간(rise time)은 9ms, 내림시간(decay time)은 9.8ms로 가장 좋은 광전도 특성을 얻었다. The ZnIn_2S_4 thin films were grown on the Si(100) wafers by a hot wall epitaxy method(HWE). The source and substrate temperature are 610℃ and 450℃ respectively. The crystalline structure of epilayers was investigated by double crystal X-ray diffraction(DCXD). Hall effect on the sample was measured by the van der Pauw method and studied on the carrier density and mobility dependence on temperature. From Hall data, the mobility was increased in the temperature range 30K to 100K by impurity scattering and decreased in the temperature range 100K to 293K by the lattice scattering. In order to explore the applicability as a photoconductive cell, we measured the sensitivity(??), the ratio of photocurrent to darkcurrent(pc/dc), maximum allowable power dissipation(MAPD), spectral response and response time. The results indicated that the photoconductive characteristic were the best for the samples annealed in S vapor compare with in Zn, In, air and vacuum vapour. Then we obtained the sensitivity of 0.99, the value of pc/dc of 1.37x10^7, the MAPD of 336mW, and the rise and decay time of 9ms and 9.8ms, respectively.

Chemical Bath Deposition 방법으로 제작한 CdSe 박막의 특성

홍광준,이상열,유상하,서상석,문종대,신영진,정태수,신현길,김택성,송정훈,유기수 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

Chemical bath deposition 방법으로 다결정 CdSe 박막을 세라믹 기판 위에 성장시킨 다음 온도를 변화시켜 열처리하고 X-선 회절무늬를 측정하여 결정구조를 밝혔다. 450℃로 열처리한 시료가 X-선 회절무늬로 부터 외삽법에 의해 a_o와 c_o 는 각각 4.302 Å과 7.014 Å인 육방정계임을 알았다. 이 때 낱알크기는 약 0.3㎛이었다. Van der Pauw 방법으로 Hall 효과를 측정하여 운반자 농도와 이동도의 온도의존성을 연구하였다. 이동도는 33 K 에서 200 K 까지는 압전산란에 의하여, 200 k 에서 293 K 까지는 극성광학산란에 의하여 감소하는 경향이 나타냈다. 광전도 셀의 특성으로 스텍트럼 응답, 감도(γ), 최대허용소비전력 및 응답 시간을 측정하였다. Polycrystalline CdSe thin films were grown on creamic substrate using a chemical bath deposition (CBD) method. They were annealed at various temperature and X-ray diffraction patterns were measured by X-ray diffractometer in order to study CdSe polycrystal structure. Using extrapolation method of X-ray diffraction patterns for the CdSe samples annealed in N_2 gas at 450℃ it was found hexagonal structure whose lattice parameters a_o and c_o were 4.302 Å and 7.014 Å, respectively. It grain size was about 0.3 ㎛. Hall effect on this sample was measured by Van der Pauw method and studied on carrier density and mobility depending on temperature. From Hall data, the mobility was likely to be decreased by piezo electric scattering at temperature range of 33 K and 200 K, and by polar optical potical scattering at temperature range of 200 K and 293 K. We measured also spectral response, sensitivily (γ), maximum allowable power dissipation and response time on these samples.

CBD 방법에 의한 CdS 박막의 성장과 광전도 특성

황광준,이상열,유상하,서상석,문종대,신영진,정태수,신현길,김택성,송정훈,유기수 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

Chemical bath deposition 방법으로 다결정 CdS 박막을 세라믹 기판 위에 성장시킨 다음 온도를 변화시켜 열처리하고 X-선 회절무늬를 측정하여 결정구조를 밝혔다. 550℃로 열처리한 시료의 경우 X-선 회절무늬로 부터 외삽법에 의해 a_。와 c_。는 각각 4.1364 Å과 6.7129 Å인 육방정계임을 알았다. 이 때 낱알크기는 약 0.35㎛이었다. Van der Pauw 방법으로 Hall 효과를 측정하여 운반자 농도와 이동도의 온도의존성을 연구하였다. 이동도는 33 K 에서 150 K 까지는 압전산란에 의하여, 150 K 에서 293 K 까지는 극성광학산란에 의하여 감소하는 경향이 나타냈다. 광전도 셀의 특성으로 스텍트럼 응답, 감도(γ), 최대허용소비전력 및 응답 시간을 측정하였다. Polycrystalline CdS thin films were grown on creamic substrate using a chemical bath deposition method. They were annealed at various temperature and X-ray diffraction patterns were measured by X-ray diffractometer in order to study CdS polycrystal structure. Using extrapolation method of X-ray diffraction patterns for the CdS samples annealed in N_2 gas at 550℃ it was found hexagonal structure whose lattice constants a_o and c_o were 4.1364 Å and 6.7120Å, respectively. Its grain size was about 0.35 ㎛. Hall effect on this sample was measured by Van der Pauw method and studied on carrier density and mobility depending on temperature. From Hall data, the mobility was likely to be decreased by piezo electric scattering at temperature range of 33K and 150K and by polar optical scattering at temperature range of 150K and 293K. We measured also spectral response, sensitivity (γ), maximum allowable power dissipation and response time on these samples.

Molecular characterization of an aldo-keto reductase from Marivirga tractuosa that converts retinal to retinol

Hong, S.H.,Nam, H.K.,Kim, K.R.,Kim, S.W.,Oh, D.K. Elsevier Science Publishers 2014 Journal of biotechnology Vol.169 No.-

A recombinant aldo-keto reductase (AKR) from Marivirga tractuosa was purified with a specific activity of 0.32unitml<SUP>-1</SUP> for all-trans-retinal with a 72kDa dimer. The enzyme had substrate specificity for aldehydes but not for alcohols, carbonyls, or monosaccharides. The enzyme turnover was the highest for benzaldehyde (k<SUB>cat</SUB>=446min<SUP>-1</SUP>), whereas the affinity and catalytic efficiency were the highest for all-trans-retinal (K<SUB>m</SUB>=48μM, k<SUB>cat</SUB>/K<SUB>m</SUB>=427mM<SUP>-1</SUP>min<SUP>-1</SUP>) among the tested substrates. The optimal reaction conditions for the production of all-trans-retinol from all-trans-retinal by M. tractuosa AKR were pH 7.5, 30<SUP>o</SUP>C, 5% (v/v) methanol, 1% (w/v) hydroquinone, 10mM NADPH, 1710mgl<SUP>-1</SUP> all-trans-retinal, and 3unitml<SUP>-1</SUP> enzyme. Under these optimized conditions, the enzyme produced 1090mgml<SUP>-1</SUP> all-trans-retinol, with a conversion yield of 64% (w/w) and a volumetric productivity of 818mgl<SUP>-1</SUP>h<SUP>-1</SUP>. AKR from M. tractuosa showed no activity for all-trans-retinol using NADP<SUP>+</SUP> as a cofactor, whereas human AKR exhibited activity. When the cofactor-binding residues (Ala158, Lys212, and Gln270) of M. tractuosa AKR were changed to the corresponding residues of human AKR (Ser160, Pro212, and Glu272), the A158S and Q270E variants exhibited activity for all-trans-retinol. Thus, amino acids at positions 158 and 270 of M. tractuosa AKR are determinant residues of the activity for all-trans-retinol.

Urokinase loading Dose와 Maintenance Dose를 위한 연구

홍세용,양동호,신현길,김순길,오도연,김기용,기은경,정광회,신상구 대한내과학회 1992 대한내과학회지 Vol.42 No.1

저자들은 two chain urokinase의 loading dose 및 maintenance dose를 결정하기 위하여 성인 뇌졸중 환자 6예를 대상으로 urokinase 300,000units를 주입하고(Ⅳ boluse), 60분간 혈중 urokinase의 변동을 관찰하여 two compartment model을 이용한 pharmacokinetic study를 하여 다음과 같은 결과를 얻었다. 체중의 55~65㎏ 정도이고 간 기능이 정상인 한국인 성인에서 urokinase의 Vc(central compartment volume)은 4.56L 이었고 반감기는 distribution phase에서 2.3분, elimination phase에서 33.1분이었다. Total boidy clearance(CL)은 244/min이었고 area under the concentration time curve(AUC)는 1,349units/㎖.min이었다. 이를 근거로 하였을때 시험관에서 free form plasmin을 유리하는 urokinase의 최소 농도인 10units를 maintenance dose로 시간당 146,400units를 주입하는 것이 적절한 것으로 판단되었으며 이는 외국 성서에서 추천하는 시간당 4,400units/㎏(60㎏×4,400=264,00units)에 비하여 약 절반 정도에 해당하는 양이다. 이렇게 차이가 나는 이유는 명확하지 않으나 본 연구에서 desired concentration으로 사용한 10units/㎖은 소위 therapeutic window의 lower margin에 해당되기 때문일 것으로 생각되며 therapeutic window의 upper magrin을 규명하기 위한 연구가 뒤따라야 할 것으로 사료된다. A Pharmacokinetic study was performed .for two-chain UK(M.W.53,000) in 6 Korean adults with body weight between 55~65㎏ Following intravenous administration of 300,000 units of UK, concentration declined rapidly. The half life showed two-compartment disposition with mean initial and terminal half-lives of 2.3 and 33.1 minutes. Total body clearance was about 244㎖/min and the steady state volume of distribution (Vdss) was 9.46L. In conjunction with our previous finding that free form plasmin begin to form at a urokinase concentration of 5~10units/㎖, our results suggests that the loading dose and maintenance dose of urokinase during systemic intravenous administration should be about 45, 610unit and 146,400unit/hr in a Korean adult.

Temperature Dependent Study of Random Telegraph Noise in Gate-All-Around PMOS Silicon Nanowire Field-Effect Transistors

Hong, B B,Choi, L,Jung, Y C,Hwang, S W,Cho, K H,Yeo, K H,Kim, D,Jin, G Y,Park, D,Song, S H,Lee, Y Y,Son, M H,Ahn, D IEEE 2010 IEEE TRANSACTIONS ON NANOTECHNOLOGY Vol.9 No.6

<P>We report the random telegraph noise observed in gate-all-around (GAA) PMOS silicon nanowire FETs (SNWFETs) with the radius of 5 nm, at various temperatures (s) down to 4.2 K. From the -dependence of the capture/emission time, we obtain the energy and the charging status of the trap states. The gate bias dependence and the -dependence of the scattering coefficient-mobility product extracted from the relative fluctuation amplitude of the drain current are consistent with the fact that the surface roughness scattering is dominant in GAA PMOS SNWFETs.</P>

불소와 생귀나리아 함유치약의 초기우식법지랑질에 대한 효과

홍석진,박기철,이상대,정성숙,김완규 단국대학교 치과대학 예방치과 1997 예방치과 세미나집 Vol.- No.11

The purpose of this study way to test potential cariostatic benifit of dentifrices containing both fluoride(F) and sanguinaria (SG) in a silica abrasive formula using microbial remin/demin cycle model. Subsurface lesion on bovine extracted enamel specimens were prepared by exposing to a lactate Carbopol buffer system for 44-hours. The specimens were assigned to 7 test groups of 12 specimens each by randomized blocks with VHN stratification. The test groups were a placebo, 2 NaF-silica dentifrice (with and without SG) and four MFP-DCPD dentifrices(different F level). Negative and positive control dentifrices were selected by the guidelines of the American Dental Association. The microbial cyclic sequence and pre- and post-treatment assays were same as described previously[Hong et al., J Dent Res 75:232, 1996]. The results of △ VHN ± S.E. for each group were; placebo, 26.70 ± 2.54; MFP(250ppm F)/DCPD, 42.34±1.90; MFP(1,000 ppm F) plus 2.0% Bamboo Salt(BS.Jukyom)/DCPD, 44.70±3.43; MFP(1,000 ppm F)/DCPD(Fresh Mint), 46.20±2.46; MFP(1,100 ppm F)/DCPD (Colgate), 55.82±4.18; NaF(1,100 ppm)/Silica(Cerst), 84.98±4.17; NaF(1,000 ppm)/Silica plus 0.1% SG(Denta Q), 92.16±5.21. The data were then statistically analyzed using ANOVA for the significance of differences among treatment groups. △ VHNs for groups treated with dentifrices containing 1,000 ppm F from MFP with or without BS were significantly higher than the placebo. The groups treated with dentifrices containing F from NaF/Silica with or without SG had significantly higher(p<0.01) △ VHNs than that with F from MFP/DCPD(p<0.01)). The △ VHNs treated with dentifrice containing both F-SG demonstrated the highest among test groups, indicating that there is an additive benefit in remineralizing the incipient subsurface lesion.

Block of hERG K⁺ channel and prolongation of action potential duration by fluphenazine at submicromolar concentration

Hong, H.K.,Hoon Lee, B.,Park, M.H.,Ho Lee, S.,Chu, D.,Jin Kim, W.,Choe, H.,Hee Choi, B.,Jo, S.H. North-Holland ; Elsevier Science Ltd 2013 european journal of pharmacology Vol.702 No.1

Fluphenazine is a potent antipsychotic drug that can increase action potential duration and induce QT prolongation in several animal models and in humans. As the block of cardiac human ether-a-go-go-related gene (hERG) channels is one of the leading causes of acquired long QT syndrome, we investigated the acute effects of fluphenazine on hERG channels to determine the electrophysiological basis for its proarrhythmic potential. Fluphenazine at concentrations of 0.1-1.0μM increased the action potential duration at 90% of repolarization (APD<SUB>90</SUB>) and action potential duration at 50% of repolarization (APD<SUB>50</SUB>) in 5min when action potentials were elicited under current-clamp conditions in guinea pig ventricular myocytes. We examined the effects of fluphenazine on hERG channels expressed in Xenopus oocytes and HEK293 cells using two-microelectrode voltage-clamp and patch-clamp techniques. The IC<SUB>50</SUB> for the fluphenazine-induced block of hERG currents in HEK293 cells at 36<SUP>o</SUP>C was 0.102μM at +20mV. Fluphenazine-induced a concentration-dependent decrease of the current amplitude at the end of the voltage steps and hERG tail currents. The fluphenazine-dependent hERG block in Xenopus oocytes increased progressively relative to the degree of depolarization. Fluphenazine affected the channels in the activated and inactivated states but not in the closed states, and the S6 domain mutation from tyrosine to alanine at amino acid 652 (Y652A) attenuated the hERG current block. These results suggest that the antipsychotic drug fluphenazine is a potent blocker of hERG channels, providing a molecular mechanism for the drug-induced arrhythmogenic side effects.

The Effects of the Selective Serotonin Reuptake Inhibitor Fluvoxamine on Voltage-Dependent K⁺ Channels in Rabbit Coronary Arterial Smooth Muscle Cells

Hong, D. H.,Li, H.,Kim, H. S.,Kim, H. W.,Shin, S. E.,Jung, W.-K.,Na, S. H.,Choi, I.-W.,Firth, A. L.,Park, W. S. PHARMACEUTICAL SOCIETY OF JAPAN 2015 Biological & pharmaceutical bulletin Vol.38 No.8

<P>We demonstrated the inhibitory effect of fluvoxamine, a selective serotonin reuptake inhibitor (SSRI), on voltage-dependent K+ (Kv) channels in freshly isolated rabbit coronary arterial smooth muscle cells using a whole-cell patch clamp technique. Fluvoxamine reduced the amplitude of Kv currents in a concentration-dependent manner with an IC50 value of 3.71 +/- 1.09 mu m and a Hill coefficient of 0.62 +/- 0.14. Although fluvoxamine did not significantly affect the steady-state activation curve, it shifted the steady-state inactivation curve toward a more negative potential. Pretreatment with another SSRI, paroxetine, did not affect the basal Kv current and did not alter the inhibitory effect of fluvoxamine on Kv channels. We concluded that fluvoxamine inhibits the Kv current in a concentration-dependent manner and in a closed (inactivated) state of the Kv channels independent of serotonin reuptake inhibition.</P>