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Yong Bin Park,김윤영,Sun Kyung Oh,Sun Gan Chung,구승엽,김석현,최영민,문신용 생화학분자생물학회 2008 Experimental and molecular medicine Vol.40 No.1
Human embryonic stem cells (hESCs) are considered to be able to stably maintain their characteristics in vitro for prolonged periods, but we had previously encountered changes in proliferative ability and differentiation potential during extended culture of hESCs. Therefore, we investigated the proliferative ability and differentiation potential of hESCs during long-term culture. The hESCs, SNUhES3, were used to analyze population-doubling time, proliferation rate and differentiation potential. We classified hESCs into three groups according to culture period. Ten colonies of hESCs for each group were daily measured colony area and population-doubling time was assessed by the changes of colony area. Proliferation rate of hESCs was measured by 5-bromo-2'-deoxyuridine (BrdU) assay and telomerase activity. To evaluate differentiation potentials for hESCs, expression levels of undifferentiated and/or differentiated hESCs markers were examined by FACS, RT-PCR and immunostaining. Population-doubling time of early passage hESCs was longer than those of middle or late passage. Proliferative ability of hESCs was accelerated depending on culture periods. Cellular morphologies and the expression level of each three germ layer markers were obviously different from each passage of reattached embryoid bodies (EBs) after spontaneous differentiation. Differentiated cells of late passage expressed higher levels of undifferentiated markers such as Oct4 and SSEA4 than those of early and middle passage. But differentiated cells of early and middle passage expressed higher level of differentiated state markers, Nestin (ectoderm), Brachyury (mesoderm), HNF3β (endoderm). From these results, it can be inferred that hESCs show higher proliferative abilities and reduced differentiation potentials as the passage number increased. Therefore, we conclude that early passage hESCs could be more suitable than middle and late passage hESCs in differentiation studies.
고속액체크로마토그라피에 의한 음양곽 중 Icariin의 정량
신국현(Kuk Hyun Shin),강삼식(Sam Sik Kang),정순간(Sun Gan Chung),조의환(Eui Hwan Cho) 한국생약학회 1989 생약학회지 Vol.20 No.1
A new method for quantitative determination of icariin in Epimedii Herba by high performance liquid chromatography was established. A reversed-phase system with a μBondapak C<sub>18</sub> column using methanol in water (15% to 70%, gradient elution) as a mobile phase was developed. Icariin and spinosin as an internal reference were detested at 350 ㎚ and the analysis was successfully carried out within 30 min.
Microtubule Inhibitory Effects of Various SJ Compounds on Tissue Culture Cells 436
Jong Han Lee,Dong Wook Kang,Ho Suk Kwon,Sun Hwan Lee,Si Kyung Park,Sun Gan Chung,Eui Hwan Cho,백순영,이주헌 대한약학회 2004 Archives of Pharmacal Research Vol.27 No.4
SJ compounds (SJ8002 and related compounds) are a group of novel anticancer agents (Cho, Chung, Lee, Kwon, Kang, Joo, and Oh. PCT/KR02/00392). To explore the anticancer mechanism of these compounds, we examined the effect of SJ8002 on microtubules of six human cell lines. At a high concentration (2 mg/mL), SJ8002 effectively disrupted microtubules of the six cell lines within 1 h. At lower concentrations (0.05~1.0 mg/mL), the antimicrotubule activity of SJ8002 varied defending on cell lines. The inhibition of in vitro polymerization of pure tubulin by SJ8002 suggested that SJ8002 acts on free tubulin, inhibits the polymerization of tubulin dimer into microtubules, and hence induces the depolymerization of microtubules.
강삼식(Sam Sik Kang),신국현(Kuk Hyun Shin),정순간(Sun Gan Chung),조의환(Eui Hwan Cho) 한국생약학회 1988 생약학회지 Vol.19 No.2
Three flavonoids were isolated from Epimedium koreanum and identified as quercetin(1), anhydroicaritin-3-O-α-rhamnoside(2) and icariin(3) by spectroscopic methods. The former two compounds are the first isolation from this plant.
Microtubule Inhibitory Effects of Various SJ Compounds on Tissue Culture Cells
Lee Jong Han,Kang Dong Wook,Kwon Ho Suk,Lee Sun Hwan,Park Si Kyung,Chung Sun Gan,Chon Eui Hwan,Paik Soon Young,Lee Joo Hun The Pharmaceutical Society of Korea 2004 Archives of Pharmacal Research Vol.27 No.4
SJ compounds (SJ8002 and related compounds) are a group of novel anticancer agents (Cho, Chung, Lee, Kwon, Kang, Joo, and Oh. PCT/KR02/00392). To explore the anticancer mechanism of these compounds, we examined the effect of SJ8002 on microtubules of six human cell lines. At a high concentration ($2\;{\mu}g/mL$), SJ8002 effectively disrupted microtubules of the six cell lines within 1 h. At lower concentrations ($0.05\~1.0\;{\mu}g/mL$), the antimicrotubule activity of SJ8002 varied defending on cell lines. The inhibition of in vitro polymerization of pure tubulin by SJ8002 suggested that SJ8002 acts on free tubulin, inhibits the polymerization of tubulin dimer into microtubules, and hence induces the depolymerization of microtubules.
A Novel Anti-cancer Agent, SJ-8029, Inhibits Angiogenesis and Induces Apoptosis
Yi Eui-Yeun,Jeong Eun-Joo,Song Hyun-Seok,Kang Dong-Wook,Joo Jeong-Ho,Kwon Ho-Seok,Lee Sun-Hwan,Park Si-Kyung,Chung Sun-Gan,Cho Eui-Hwan,Kim Yung-Jin The Korean Society for Biomedical Laboratory Scien 2006 Journal of biomedical laboratory sciences Vol.12 No.3
A new piperazine derivative, 8J-8029, is a synthetic anti-cancer agent which exhibits both microtubule and topoisomerase II inhibiting activities. In this study, we investigated the ability of 8J-8029 for anti-angiogenesis and apoptosis. 8J-8029 decreased the bFGF-induced angiogenesis in the CAM and the mouse Matrigel implants, in vivo. 8J-8029 inhibited the proliferation, migration, invasion, tube fonnation, and expression of MMP-2 in BAECs. In addition, 8J-8029 reduced the cell viability in HepG2 cells, caused the production of fragmented DNA and the morphological changes corresponding to apoptosis. 8J-8029 also elicited the release of cytochrome c and the activation of caspase-3. Taken together, these results suggest 8J-8029 may be a candidate for anti-cancer agent with the ability to inhibit the angiogenesis of endothelial cells and to induce the apoptosis of tumor cells.