열처리 방법에 따른 표고버섯의 이화학적 특성 및 영양학적 분석

이중규(Jung-Gyu Lee),김광일(Kwang-Il Kim),황인국(In-Guk Hwang),유선미(Seon-Mi Yoo),민상기(Sang-Gi Min),최미정(Mi-Jung Choi) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.6

본 연구는 열수 침지, 증기 및 볶음 처리가 표고버섯에 미치는 이화학적 분석 및 영양성분 분석을 통해 최적 열처리 조건을 확립하고자 진행하였다. 소비자들이 상품을 평가할 때 1차적으로 먼저 관찰되는 색도와 경도를 측정하고 추가로 pH 측정 등으로 인한 이화학적 결과로 최적 조건을 선정한 뒤에 그 조건들에 대해서 영양학적 및 미생물 검사를 실시하였다. 색도는 열처리 시간이 길어질수록 색의 변색 정도가 증가하여 뚜렷한 차이를 나타내었다. pH 값은 열수 침지 처리의 경우 pH 증가량이 가장 높았으며 처리 시간이 지속될수록 pH가 증가하는 경향을 나타내었다. 다른 처리군의 경우 일정 시간의 처리 시간 이후에는 유의적인 차이를 보이지 않았다. 경도에서는 열수 침지와 볶음 처리의 경우 1분 이내의 열처리만으로도 경도가 감소하는 경향을 보였으며, 증기처리의 경우 1분 동안 경도가 유지되는 결과를 보였다. 일반성분 분석 결과는 증기 처리가 가장 원물에 가까운 결과를 나타냈다. 유기산 함량은 증기 처리에서 유기산 함량 감소율이 가장 낮았다. 또한 3분간 증기 처리를 하였을 때 미생물의 사멸 효과가 가장 높았다. 열수 침지 처리나 볶음 처리의 경우 전체적으로 증기 처리한 시료에 비해서 영양성분 함량이 줄어드는 경향을 나타냈다. 따라서 표고버섯을 증기 처리방법으로 열처리하는 것이 다른 열처리군에 비해서 최적으로 나타났다. 하지만 본 연구에서는 물리화학 및 영양학적 성분의 변화만을 분석하였기 때문에 실제 식품 산업이나 공정에 적용하기 위해서는 대용량 처리 시에 발생하는 문제들과 관련된 추가적인 연구를 필요로 할 것이다. In the food industry, thermal treatment is an important process for extending shelf-life of foods. However, heating process affects the physicochemical, nutritional, and microbial properties of foodstuff such as color, texture, pH, and proximate compositions. This study was conducted to select an optimal thermal treatment by observing physicochemical, nutritional, and microbial effects of shiitake mushrooms with different thermal treatment methods. Shiitake mushrooms were washed and sliced equally (5 cm×0.5 cm×0.5 cm) and then heat-treated by three methods. Samples were heated in 100°C boiling water, steamed for 10 min, or pan fried at 130°C for 4 min. Total color difference values showed significantly increasing tendency with treatment time. For pH values, boiling water-treated mushrooms showed increasing tendency according to increased thermal treatment. For the results of hardness, boiling water or pan frying-treated mushrooms showed reduced tendency within 1 min. In the case of steam-treated mushrooms, hardness values were maintained for 1 min. Organic acid contents of steam-treated sample showed the lowest value among treatments. For microbial counts, steam-treated samples for 3 min showed the lowest value. Consequently, the results of this study suggest that steam treatment could be the optimal thermal treatment to minimize quality loss of shiitake mushrooms.

Effects of Dilute Acid Pretreatment on Enzyme Adsorption and surface Morphology of Liriodendron tulipifera

Byeong Cheol Min,Bon Wook Koo,Ki Seob Gwak,Hwan Myeong Yeo,Joon Weon Choi,In Gyu Choi 한국목재공학회 2011 목재공학 Vol.39 No.2

In this study, dilute acid pretreatment of Liriodendron tulipifera was performed for enzymatic hydrolysis. As the pretreatment temperature was increased, enzymatic hydrolysis and enzyme adsorption yield also increased. The highest enzymatic hydrolysis yield was 57% (gig) and enzyme adsorption was 44% (gig). Enzymatic hydrolysis yield was determined with weight loss of pretreated biomass by enzyme, and enzyme adsorption was a percentage of enzyme weight attaching on pretreated biomass compared with input enzyme weight. When L. tulipifera was pretreated with 1% sulfuric acid at 160℃ for 5 min., hemi-cellulose was significantly removed in pretreatment, but the lignin contents were constant. Other changes in surface morphology were detected on biomass pretreated at 160℃ by a field emission scanning electron microscope (FESEM). A large number of spherical shapes known as lignin droplets were observed over the entire biomass surface after pretreatment. Hemicellulose removal and morphological changes improved enzyme accessibility to cellulose by increasing cellulose exposure to enzyme. It is thus evidence that enzyme adsorption is a significant factor to understand pretreatment effectiveness.

Purification and Characterization of Helicobacter pylori ${\gamma}$-Glutamyltranspeptidase

Song, Jae-Young,Choi, Yeo-Jeong,Kim, Jeong-Min,Kim, Yoo-Ree,Jo, Jin-Seong,Park, Jin-Sik,Park, Hee-Jin,Song, Yun-Gyu,Lee, Kon-Ho,Kang, Hyung-Lyun,Baik, Seung-Chul,Youn, Hee-Shang,Cho, Myung-Je,Rhee, Kw The Korean Society for Microbiology 2011 Journal of Bacteriology and Virology Vol.41 No.4

Gamma-glutamyltranspeptidase (GGT) was purified to electrophoretic homogeneity from the cell extract of H. pylori. The purified enzyme consisted of heavy and light subunits with molecular weights of 38 kDa and 21 kDa, respectively. N-terminal amino acid sequence of heavy and light subunits revealed that H. pylori GGT was processed into 3 parts for a signal peptide of 27 amino acid residues, a heavy subunit of 352 residues, and a light subunit of 188 residues during translation. The reaction rate for hydrolysis of ${\gamma}$-GpNA was 84.4 ${\mu}mol/min$ per milligram of protein, and that for the ${\gamma}$-glutamyl transfer from ${\gamma}$-GpNA to gly-gly was 23.8 ${\mu}mol/min$ per milligram of protein. The apparent Km values of H. pylori GGT for ${\gamma}$-glutamyl compounds were on the order of $10^{-3}$ to $10^{-4}$ M and those for acceptor peptides and amino acids were on the order of $10^{-1}$ to $10^{-2}$ M. The GGT protein kept approximately 80% of the initial enzymatic activity on incubation at $60^{\circ}C$ for 15 min. The optimum temperature and pH for reactions of both hydrolysis and transpeptidation were $40^{\circ}C$ and 9.0, respectively. The transpeptidation and hydrolysis reactions catalyzed by H. pylori GGT were strongly inhibited by L-Gln and moderately inhibited by L-Ala, L-Ser, ${\beta}$-chloro-L-Ala, and L-Glu. These results demonstrated that the biochemical properties of H. pylori GGT are different from those of other bacterial GGTs. Further, H. pylori GGT might degrade glutathione in the gastric mucous layer of humans if the enzyme could be secreted in the bacterial niches.

Sustained-release praziquantel tablet : pharmacokinetics and the treatment of clonorchiasis in beagle dogs

Hong, Sung-Tae,Lee, Sang Hyup,Lee, Seung-Jin,Kho, Weon-Gyu,Lee, Mejeong,Li, Shunyu,Chung, Byung-Suk,Seo, Min,Choi, Min-Ho 梨花女子大學校 藥學硏究所 2003 藥學硏究論文集 Vol.- No.12

Praziquantel is rapidly absorbed and secreted; and thus fractional doses are recommended for the treatment of cestode and trematode infections. In the present study, we developed a new praziquantel tablet formula allowing sustained-release (SRP). In vitro dissolution of SRP tablets showed that praziquantel at 300 ㎎/tablet combined with hydroxypropyl methylcellulose dissolved completely at a constant rate over 10 h, whereas the conventional praziquantel tablet (PZQ) was only 40% dissolved. Pharmacokinetic studies in dogs confirmed that SRP was absorbed more slowly than PZQ. The mean value of the area under the concentration/time curve from 0 h to the final observation time, the maximum concentration in serum, and the time of maximum concentration in serum for SRP were 3.471.500 ng/min for 0.25 ㎖, 10,300 ng for 0.25 ㎖, and 192 min. while the values for PZQ were 688,600 ng/min for 0.25㎖, 2,500 ng for 0.25㎖, and 135 min. The cure rate in dogs with a heavy infection (500 metacercariae) treated with a single dose of SRP (150 ㎎/tablet) at 50 ㎎/㎏ was 80%, while in dogs treated with a single dose of SRP (300 ㎎/tablet) at 30 mg/kg it was 60%, and the cure rate with PZQ was 20%. In each case. the egg reduction rate was similar (over 90%). No abnormal liver functions or hepatic or renal pathologies were observed in dogs administered with SRP at 30 ㎎/㎏. The SRP tablet showed sustained release and slow absorption; and it had an improved anthelmintic efficacy against Clonorchis sinensis in experimental dogs. compared with conventional praziquantel.

벼 밭재배에 따른 수량 및 미질

최원영,최민규,김상수,이규성,김태수,이재길 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.4

벼 밭재배시 논벼 품종들의 적응성을 검토하기 위하여 마늘 간작으로 벼를 건답점파하여 재배한 결과를 요약하면 다음과 같다. 1. 입모율은 밭벼에 비해 논벼 품종이 낮았는데 그 순서는 상남밭벼〉동진찰벼〉신선찰벼〉진부찰벼 순이었다. 2. 출수기는 상남밭벼에 비해 진부찰벼는 3일 빨랐고 신선찰벼는 9일, 동진찰벼는 12일이 늦었다. 3. ㎡당 수수와 등숙비율은 밭벼에 비해 논벼품종들이 높았다. 4. 쌀수량은 상남밭벼에 비해 신선찰벼는 12%가 많았고 동진찰벼는 같았으며, 진부찰벼는 18%가 적었다. 5. 완전립비율은 진부찰벼에서 높았고 그 외는 비슷하였으며, 식미지수는 상남밭벼에 비해 동진찰벼는 높았으나 진주찰벼와 신선찰벼는 낮았다. To identify the rice varieties adaptable for upland cultivation, a series of experiments was carried out at the field(Chonnam series) of the Muan. Cheonnam province during 1999 - 2001. Four varieties, Jinbuchalbyeo(early-maturing variety), Sinseonchalbyeo (medium-maturing variety), Dongjinchalbyeo(medium-late maturing variety), and Sangnambatbyeo as a standard variety for upland were used in this experiment. Seedling establishment of the varieties was the highest in Sangnambatbyeo and followed by Dongjinchalbyeo under upland condition. Heading date in general delayed ranges from 9 to 12 days, while early-maturing variety, Jinbuchalbyeo was faster for 3 days compared with Sangnambatbyeo. Number of panicle per square meter was similar in both lowland and upland rice but percentage of ripened grain was high in lowland rice. Milled rice yield was high as of 12% in Sinseonchalbyeo compared with Sangnambatbyeo. Head rice ratio showed also the highest in Sinseonchalbyeo. The results indicate that Sinseonchalbyeo as glutinous rice variety could adapt well in this upland condition, and have good milling recovery as head rice as well as yield advantage.

活性슬러지工程의 最適 運轉因子에 關한 硏究 -江陵下水處理場을 中心으로

崔善道,曺圭敏 三陟大學校 2001 論文集 Vol.34 No.2

A sewage treatment plant which has been operated by the treatment process of conventional activated sludge process and its optimal operating factors depends on a condition of its design and environment. This study was carried out by experiments to exhibit optimal operating factors of the Sludge process. The sewage was inflowed regularly but in winter. The concentration of Inflow water appeared that of spring/summer is higher than that of autumn/winter. The removal rate of BOD/SS sppeared in the order of summer>spring>autumn>winter. MLSS was maintained high in winter/spring, low in summer/autumn, because it changes according to the temperature of the aeration tank and pollutional concentration of the inflow water. The optimal operating factors can improve the process efficiency and operate easily at the sewage treatment plant. The results of this study were summarized as follow; 1) In the winter, it has been measured that DO is 1.5mg/ℓ, MLSS is 2,191mg/ℓ, SV?? Is 15%, a load of BOD-SS(F/M ratio) is 0.16, SVI is 68, SRT is 10 day, the return rate of sludge is 44%. 2) In the spring, it has been measured that DO is 2.3mg/ℓ, MLSS is 2,047mg/ℓ, SV??is 21%, a load of BOD-SS(F/M ratio) is 0.15, SVI is 106, SRT is 6 day, the return rate of sludge is 37%. 3)In the summer, it has been measured that DO is 0.8mg/ℓ, MLSS is 1,653mg/ℓ, SV?? is 8.5%, a load of BOD-SS(F/M ratio) is 0.3, SVI is 51.6, SRT is 4 day, the return rate of sludge is 33%. 4) In the autumn, it has been measured that DO is 0.7mg/ℓ, MLSS is 1,560mg/ℓ, SV?? is 9.7%, a load of BOD-SS(F/M ratio) is 0.17, SVI is 62, SRT is 7.91 day, the return rate of sludge is 38%.

절충연산자를 이용한 다목적 비선형 계획법의 대화형 퍼지 의사결정

최규탁,남현우,박재민 慶南專門大學 1997 論文集 Vol.25 No.1

BCR-ABL 융합유전자를 동반한 급성 골수구성 백혈병 1예

최정민,송성은,이규진,이규원,이호섭 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.1

BCR-ABL 융합 유전자를 동반한 급성골수구성백혈병은 그 빈도가 매우 낮고, 만성골수구성백혈병의 급성악화기와 임상적으로 유사한 특징을 가진다. 항암화학요법으로 관해유도가 되기 어렵거나, 관해유도 후 재발의 빈도가 높은 특징을 가지고 있어 예후가 나쁘다고 알려져있다. 저자들은 BCR-ABL 융합 유전자를 동반한 급성골 수구성백혈병1례를 경험하였기에 문헌고찰과 함께 보고 하는 바이다. BCR-ABL fusion gene is the hallmark of chronic myelogenous leukemia (CML), but it has been rarely reported in acute myeloid leukemia (AML). AML with BCR-ABL fusion gene has different features from blast crisis of CML. We experienced a rare case of AML with BCR-ABL fusion gene. Here we report this case with a brief review of literatures.

수근관 증후군의 국소 스테로이드 주사

조민규,이성훈,강은영,전평식,최영태 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.5

다발성 간종괴의 형태로 나타난 특발성 과호산구 증후군 치험1예

최승오,연규민,이승민,김순제,왕준호,박형석,이재동,진춘조,이창훈,신현준,김정은 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

Hypereosinophilic syndrome is characterized by persistent blood eosinophilia of 1,500/ul or more in the absence of known causes and multiorgan dysfunction by eosinophil-related tissue damage. In Korea, some cases of hypereosinophilic syndrome with hepatic involvement have been described with prolonged benign clinical courses. Most of them were diffuse or multifocal lesions in imaging studies, and a few case presenting as a single mass has been described. Here we report a case of hypereosinophilic syndrome with hepatic involvement in a 65-year-old man who presented with multifocal mass lesions. By abdominal US and CT scan, multiple, ill-defined nodules were detected in throughout the liver. Liver biopsy revealed severe eosinophilic hepatitis, suggestive of hypereosinophilic syndrome. Hepatic mass lesions improved after steroid administration for several weeks.