S-528 : The Clinical Characteristics of Angioedema with Eosinophilia

( Hyo Jung Cho ),( Hye Soo Yoo ),( Mi Ae Kim ),( Yoo Seob Shin ),( Young Min Ye ),( Dong Ho Nahm ),( Joo Hee Kim ),( Jeong Hee Choi ),( Sun Young Park ),( Hae Sim Park ) 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1

Angioedema with eosinophilia (AE) is a very rare allergy disease, case reports of which have been published sporadically since 1984. Here, we retrospectively analyzed the clinical features of 10 AE patients in Korea. Nine of the 10 subjects were young females, ranging from 23 to 38 years old. Twenty percent of the subjects had episodic-type AE with high serum IgM and eosinophil counts, while 80% were non-episodic type with normal serum IgM levels but high eosinophil counts. All patients had used systemic corticosteroids to control AE. One patient with refractory EAE was treated with anti-IgE antibody. This is the first study to evaluate the clinical characteristics of AE in a Korean population.

자색고구마가 사염화탄소 투여에 의한 흰쥐의 간손상 보호에 미치는 영향

조영자,김현아,방미애,오용비,정병춘,문윤호,정우진 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.3

The purpose of this study was to investigate the effects of dietary purple sweet potato(lpomoea batatas) powder on serum lipid levels and antioxidative enzymes in normal and pretective effect on hepatotoxicity rats induced by carbon tetrachlolide. Four groups of rats (3-week-old inbred Sprague-Dawley male rats) were normal rats fed control diet(C)_(1) induced hepatotoxicity rats fed control diet(EC)_(1) normal rats fed purple sweet potato diet(P), and induced hepatotoxicity rats fed purple potato sweet diet(EP). Rats were induced by single injection of 50% carbon tetrachloide(0.1 mL/100 g B.W., i.p.). The rats were fed ad libitum each of the experimental diet for 5 weeks. After 5 weeks the rats were sacrificed and activities of antioxidant enzymes and lipid peroxidation products were determined in their liver homogenates. But serum concentrations of lipid was not significant in all groups. Serum alanine aminotransferase(ALT/GPT) and aspartate aminotransferase(ALT/GPT)of the EC and EP groups were heigher than the C and P groups. The hepatic glucose 6-phosphatase(G9Pase) activity of the group fed purple potato diet(P) was lower than the other groups(p<0.05). However, The glutathione peroxidase(GPx) activities was not statistically different between the groups. Renal glutathione S-transferase(GST) activity of the EC and EP groups were lower than the C and P groups(p<0.05). In conclusion, these results suggest that purple sweet potato is believed to be possible protective effect on hepatotoxicity rats induced by carbon tetrachlolide.

복합레진 수복 시 재접착 술식이 미세누출에 미치는 영향

이미애,서덕규,손호현,조병훈 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.3

복합레진 수복시, 일반적으로 연마, 산부식, 수세 및 건조 단계를 거쳐 저점도 레진을 적용하는 재접착 술식을 통해 예상되는 수축간극을 봉쇄한다. 그러나, 이 과정은 재접착제 적용 이전에 물이나 잔사가 간극을 매워버릴 수 있어 그 효과가 의심된다. 본 실험에서는 연마 단계 이전, 즉 복합레진을 중합한 직후에 재접착제를 도포한다면 변연누출을 더 줄일 수 있을 것이라는 가설을 검증하고자 하였다. 실험을 위해 35개의 발거한 대구치에서 협면과 설면에 교합면 변연은 법랑질에, 치은측 변연은 상아질에 위치하는 5급 와동을 형성하였다. 와동은 Z250 (3M ESPE, USA)로 충전하였고, 접착제로는 AdperTM Single Bond 2(3M ESPE)을 사용하였다. 재접착제로는 Biscover LV (Bisco, USA) 레진 전색제와 ScotchBond Multi-purpose system(3M ESPE)의 접착제, 및 점도가 낮은 실험용 접착제를 연마 전 또는 일련의 연마 및 산부식 후 수복물의 변연에 적용하였다. 2% 메틸렌 블루 용액에 네 시간 동안 침적한 뒤 교합측 및 치은측 변연에서의 색소 침투 깊이를 광학 입체 현미경으로 측정하였다. 재접착제의 점도와 변연미세누출의 상관관계도 평가하였다. 재접착 술식, 재접착제, 및 변연의 위치, 그 각각에서는 통계적으로 유의한 차이를 관찰할 수 없었다. 그러나, 재접착 술식을 시행하지 않은 대조군의 경우에는 세 가지 재접착제를 사용한 재접착군에 비해 치은측 변연에서 미세누출이 통계적으로 유의하게 크게 나타났다(p < 0.05). 대조군과 재접착군의 미세누출의 차이는 교합측 변연에서는 관찰되지 않았다. 연마 및 산부식 후 치은변연에 재접착제를 적용한 경우에서 약한 음의 상관관계(r = -0.326, p = 0.041)를 보인 경우를 제외하고는, 재접착제의 점도와 미세누출은 유의한 상관관계를 보이지 않았다. During a composite resin restoration, an anticipating contraction gap is usually tried to seal with low-viscosity resin after successive polishing, etching, rinsing and drying steps, which as a whole is called rebonding procedure. However, the gap might already have been filled with water or debris before applying the sealing resin. We hypothesized that microleakage would decrease if the rebonding agent was applied before the polishing step, i.e., immediately after curing composite resin. On the buccal and lingual surfaces of 35 extracted human molar teeth, class V cavities were prepared withthe occlusal margin in enamel and the gingival margin in dentin. They were restored with a hybrid composite resin Z250 (3M ESPE, USA) using an adhesive AdperTM Single Bond 2 (3M ESPE). As rebonding agents, BisCover LV (Bisco, USA), ScotchBond Multi-Purpose adhesive (3M ESPE) and an experimental adhesive were applied on the restoration margins before polishing step or after successive polishing and etching steps. The infiltration depth of 2% methylene blue into the margin was measured using an optical stereomicroscope. The correlation between viscosity of rebonding agents and mciroleakage was also evaluated. There were no statistically significant differences in the microleakage within the rebonding procedures, within the rebonding agents, and within the margins. However, when the restorations were not rebonded, the microleakage at gingival margin was significantly higher than those groups rebonded with 3 agents (p < 0.05). The difference was not observed at the occlusal margin. No significant correlation was found between viscosity of rebonding agents and microleakage, except very weak correlation in case of rebonding after polishing and etching at gingival margin (r = -0.326, p = 0.041).

Hollow-Fiber LPME를 이용한 잔류농약 검출

조현우,이상섭,신윤지,장미애 경기대학교 기초과학연구소 2008 기초과학논문집 Vol.21 No.-

A method for the determination of trace amount of pesticides in aqueous samples using hollow fiber-liquid phase microextraction(HF-LPME) and capillary gas chromatograph/micro electron capture detector(GC/μ ECD) has been established. HF-LPME method has been optimized with respect to several experimental parameters including the effects of extraction solvent, extraction time and extraction temperature for the determination of pesticides from aqueous samples. The calibration curves for pesticides showed good linearity (above r²=0.98). The established method is feasible for the determination of trace amounts of pesticides in several aqueous sample. The limit of detection(LOD) and the limit of quantitation(LOQ) have been found to be 0.05~1.0 and 0.5~2.5 ng/mL, respectively. The established HF-LPME method for the analysis of pesticides from aqueous sample can be used for the determination of biological, food and environmental samples.

다시마 분말이 당뇨 유발쥐의 혈당과 지질농도 및 항산화 효소계에 미치는 영향

조영자,방미애 한국식생활문화학회 2004 韓國食生活文化學會誌 Vol.19 No.4

The purpose of this study was to investigate the effect of dietary sea tangle in diabetic rats treated with streptozotocin(STZ). Four groups of rats (Sprague-Dawley male rats, 180-200g) were normal rats fed control diet(C), diabetic rats fed control diet(CD), normal rats fed sea tangl diet(T), and diabetic rats fed sea tangle diet(TD). Diabetes was induced by single injection of streptozotocin(60㎎/㎏ B.W.). High density lipoprotein(HDL) of T and TD group were higher than other groups(C and T groups). And the weekly change of blood sugar was decreased in the 3th and 4th weeks. But serum triglyceride (TG) of diabetic rats fed sea tangle diet(TD) was lower than diabetic rats fed control diet(CD). Activity of hepatic microsomal Glucose 6-phosphatase(G6Pase) was significantly increased CD and TD groups higher than C and T groups. Hepateic glutathione S-transferase(GST) of T, CD and TD groups were significantly lower than C group(p〈0.05), glutathione peroxidase (GPX) of T and TD groups were significantly higher than C and CD groups(p〈0.05).

식이 중 인동초를 섭취한 당뇨유발 흰쥐의 혈당 및 지질대사

조영자,김현아,방미애 목포대학교 생활과학연구소 2000 생활과학논집 Vol.3 No.-

The purpose of this study was to invesgate the effect of L. japonica tumbo(P) on blood glucose level and lipid metabolism in streptozotocin(STZ) induced diabetic rats. Male Sprague-Dawley rats weighing(180~200g)were divided into four groups: control(C), STZ-control(CD), and two experimental groups(P and PD). Diabetes mellitus were induced by single injection of the streptozotocin at tue dose of 60mg/kg B.W. The experimental groups were fed a AIN-76 recomendation diet mixed with the P powder(5%/kg diet). The diet and water were intake determined everyday. The blood glucose level was determined everyweek, and the serum cholesterol was determined every two weeks. After 5 weeks, the rats sacrificed and the blood glucose, serum cholesterol. triglyceride, HDL-cholesterol concentrations were determined. LDL-cholesterol concentrations was calculated by eqation. Diabetic rats showed the lower weight gain compared to the normal rats. The serum glucose level of PD was not lower than C group. However, the serum cholesterol, triglyceride and HDL-cholesterol level of CDI and PD group were heigher than C group. Diabetic rats showed that the hepartic glutathione reductase(GR) was sighificantly heigher than C group, but malondialdehyde level was significantly decreased. P supplymentation was reduced the hepartic malondialdehyde(MDA) level. The glutathione reductase(GR) and HDL-cholestrol level were increased in the diabetic experimental groups compared C group. In comculusion, showing the possibility of preventive and therpeutic use of the wild edible plant(Indong- Cho) to the diabetes mellitus.

당뇨 유발쥐에서 인동초의 섭취가 혈청지질과 혈당 및 항산화효소계에 미치는 영향

방미애,조영자,김현아 한국식생활문화학회 2002 韓國食生活文化學會誌 Vol.17 No.4

The purpose of this study was to investigate the effects of dietary Indongcho(L. japonica Thunb) powder on blood glucose, serum lipid levels and antioxidative anzymes in normal and streptozotocin(STZ)-induced diabetic rats. Four groups of rats(2-week-old inbred Sprague-Dawley male rats) were normal rats fed control diet(NC), diabetic rats fed control diet(DC), normal rats fed Indongcho powder diet(NI), and diabetic rats fed Indongcho powder diet(DI). Diabetes was induced by single injection of streptozotocin(60mg/kg BOW., i.p.). The animals were fed ad libium each of the experimental diet for 5 weeks. Food and water intakes were determined everyday. Blood glucose and serum total cholesterol levels were determined every week. After 5 weeks the animals were sacrificed and activities of antioxidant enzymes and lipid peroxidation products were determined in their liver and kidney homogenates. We also determined serum concentrations of total lipid(TL), total cholesterol(TC), triglycerides(TG) and HDL-cholesterol(HEL-C).Blood sugar and water intake were higher in decreased by dietary Indongcho intake, Body weight gain and FER(feed efficiency ration) were reduced by STZ treatment. But, Final body weight was recovered by Indongcho-contained diet. LHR(LDL-cholesterol/HDL-cholesterol) of the DI group was significantly lower than the other experimental groups(NC, NI and DE groups). The hepatic glucose 6-phosphatase(G6Pase) activity of the groups fed Indongcho diet(NI and DI group) was lower than the groups fed control diet(NC and DC group) and the G6Pase activity of NI group was recovered to the normal levels(p<0.05). However, The glutathione peroxidase(GPx) and glutathione reductase(GR) activities in liver and G6Pase activity in kidney were not statistically different between the control and diabetic control groups. Renal GST activity of the DI group was recovered by Indongcho intake. In conclusion, these results confirm oxidative stress in the liver and kidney of rats with STZ diabetes and antioxidative effect of Indongcho.

서울·경기지역 병원직원의 혈액매개 감염노출에 대한 다기관 조사연구

박미라,김정은,박은숙,최정실,정선영,송영구,홍성관,김준명,허애정,양동규,조영주 대한병원감염관리학회 2003 의료관련감염관리 Vol.8 No.1

경구용 서방성/지연성 성형제품의 허가 후 변경사항 관리를 위한 SUPAC-MR 응용

사홍기,조미현,박상애,윤미옥,강신정 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.3

The objective of this study was to scrutinize the rationale of SUPAC-MR and its application in processing postapproval changes to modified release solid oral dosage forms. The types of postapproval changes that were primarily covered with SUPAC-MR included variations in the components and composition, the site of manufacturing, batch size, manufacturing equipment, and manufacturing process. SUPAC-MR defined levels of postapproval changes that the industry might make. Classification of such categories was based on the likelihood of risk occurrence and potential impact of changes upon the safety and efficacy of approved drug products. In most cases, the changes could be classified into 3 levels. It described what chemistry, manufacturing, and control tests should be conducted for each change level. The important tests specified in SUPAC-MR were batch release, stability, in vitro dissolution, and in vivo bioequivalence tests. It then suggested what type of a filing report should be submitted to the FDA for each change level. In general, level 1 changes could be reported in an annual report, whereas level 2 and/or 3 changes could be submitted in changes-being-effected or prior approval supplements. It could be understood that the purpose of SUPAC-MR was to maintain the safety and quality of approved modified release solid oral dosage forms undergoing certain changes. At the same time, it contributed to providing a less burdensome regulatory process with the manufacturers when they wanted to make postapproval changes. European regulatory agencies also implemented SUPAC-like regulations in handling such changes to drug products. Therefore, in this study a recommendation was made for KFDA and the Korean industry to evaluate thoroughly the usefulness of these guidances and regulations in dealing with postapproval changes to modified release solid oral dosage forms.

다시마 분말을 첨가한 막장 에탄올 추출물의 항돌연변이원성 및 세포독성 효과

최승필,조미애,전윤영,이득식,함승시 동아시아식생활학회 2002 동아시아식생활학회지 Vol.12 No.1

This study was carried out to investigate antimutagenic and cytotoxic effects of Korean traditional Mackjang added with sea tangle powder. The content percentage of most of minerals among general composition increased by adding sea tangle powder. By the Ames test, the antimutagenic effect of ethanol extract of Korean traditional Mackjang added with 5% sea tangle powder was strongest, exceeding the control, 10% and 15% sea tangle additions. The ethanol extract (400 ㎍/plate) of Mackjang added with 5% sea tangle powder in the S. typhimurium TA100 strain showed inhibition rate of 95.0% against the mutagencsis induced by MNNG. The inhibition rate of ethanol extract (400 ㎍/plate) of Mackjang added with 5% sea tangle powder in the S. typhimurium TA98 and TA100 strains was 81.4% and 88,8%, respectively, against the mutagencsis induced by 4NQO. Cinder the same conditions, the suppression against B(a)P and Trp-P-1 in the TA98 and TA100 strains were 85.3% and 91.0%, and 96.5% and 92.0%. respectively. For the anticancer effects, an investigation was done on the cytotoxicity of Mackjang added with 5% sea tangle powder on the cell lines with human lung carcinoma (A549), human hepatoeellular carcinoma (HepG2), and human gastric carcinoma (KATOIII). The cytotoxicities were inhibited with increasing the extract concentration. The treatment of 1.0 ㎎/mL Mackjang added with 5% sea tangle powder showed relatively strong cytotoxicity of 61.2%, 61.8% and 66.8% against A549, KATOIII, and HepG2, respectively.