당뇨 유발쥐에서 인동초의 섭취가 혈청지질과 혈당 및 항산화효소계에 미치는 영향

방미애,조영자,김현아 한국식생활문화학회 2002 韓國食生活文化學會誌 Vol.17 No.4

The purpose of this study was to investigate the effects of dietary Indongcho(L. japonica Thunb) powder on blood glucose, serum lipid levels and antioxidative anzymes in normal and streptozotocin(STZ)-induced diabetic rats. Four groups of rats(2-week-old inbred Sprague-Dawley male rats) were normal rats fed control diet(NC), diabetic rats fed control diet(DC), normal rats fed Indongcho powder diet(NI), and diabetic rats fed Indongcho powder diet(DI). Diabetes was induced by single injection of streptozotocin(60mg/kg BOW., i.p.). The animals were fed ad libium each of the experimental diet for 5 weeks. Food and water intakes were determined everyday. Blood glucose and serum total cholesterol levels were determined every week. After 5 weeks the animals were sacrificed and activities of antioxidant enzymes and lipid peroxidation products were determined in their liver and kidney homogenates. We also determined serum concentrations of total lipid(TL), total cholesterol(TC), triglycerides(TG) and HDL-cholesterol(HEL-C).Blood sugar and water intake were higher in decreased by dietary Indongcho intake, Body weight gain and FER(feed efficiency ration) were reduced by STZ treatment. But, Final body weight was recovered by Indongcho-contained diet. LHR(LDL-cholesterol/HDL-cholesterol) of the DI group was significantly lower than the other experimental groups(NC, NI and DE groups). The hepatic glucose 6-phosphatase(G6Pase) activity of the groups fed Indongcho diet(NI and DI group) was lower than the groups fed control diet(NC and DC group) and the G6Pase activity of NI group was recovered to the normal levels(p<0.05). However, The glutathione peroxidase(GPx) and glutathione reductase(GR) activities in liver and G6Pase activity in kidney were not statistically different between the control and diabetic control groups. Renal GST activity of the DI group was recovered by Indongcho intake. In conclusion, these results confirm oxidative stress in the liver and kidney of rats with STZ diabetes and antioxidative effect of Indongcho.

Streptozotocin - 유발당뇨쥐에서 함초첨가 식이의 항당뇨 및 항산화 효과

방미애(Mi-Ae Bang),김현아(Hyeon-A Kim),조영자(Young-Ja Cho) 한국식품영양과학회 2002 한국식품영양과학회지 Vol.31 No.5

염생 식물인 함초의 기능성을 규명하기 위하여 S.D.계 백서에 당뇨를 유도시킨 후 5% 함초 분말 식이로 5주간 실험 사육한 후 혈청지질ㆍ혈당농도와 주요장기의 당 대사 및 항산화 효소의 활성 변화활성을 측정하여 함초의 섭취가 당질ㆍ지질대사 및 항산화 효소계에 미치는 영향을 조사하였다. 당뇨군에서 함초의 섭취는 식이효율을 증가시켜, 당뇨에 의해 나타나는 체중감소현상을 억제하여 당뇨의 증세를 완화시켰다. 또한 함초의 섭취는 혈당강하효과 및 혈청 총 지질과 중성지방 저하효과를 보였다. 따라서 함초의 섭취는 당뇨쥐의 지질상승억제 및 혈당 저하 효과로 항당뇨 효능을 나타낼 수 있을 것으로 사료된다. 또한 당뇨합병증의 발생기전을 항산화효소와 관련시켜 연구하고자, 간과 신장의 항산화관련 인자를 측정한 결과, 당뇨에 의해서 증가된 간 G6Pase와 신장 GST의 활성도가 함초 섭취에 의해 정상수준으로 회복되었다. 또 GR의 활성도는 함초 당뇨군(DH)의 간조직에서는 증가하였으나 신장에서는 오히려 감소하였다. 따라서 본 연구는 함초 섭취가 고혈당 및 고지혈증을 억제하고 당뇨로 인한 항산화 효소 활성변화를 정상으로 회복시킴을 밝혀 함초를 활용한 건강식품개발 위한 가능성을 제시하는 바이다. Male Sprague-Dawley rats were blocked into four groups which were normal rats fed control diet (NC), diabetic rats fed control diet (DC), normal rats fed Hamcho powder diet (NH), and diabetic rats fed Hamcho powder diet (DH). Diabetes was induced by single injection of streptozotocin (60 mg/kg B.W., i.p.). The animals were fed ad libitum for 5 weeks. Malondialdehyde (MDA), glucose 6-phosphtase (G6Pase), glutathione S-transferase (GST), glutathione peroxidase (GPx), and glutathione reductase (GR) activities were measured in the homogenates of liver and kidney, and total lipid, total cholesterol, triglyceride, and HDL-cholesterol concentrations in the blood serum. Food and water intakes were markedly higher in diabetic groups than those of normal groups and were not significantly decreased by Hamcho powder supplementation. But, FER (Feed efficiency ratio) of DH group was higher than that of DC group. Total cholesterol level of DH group was decreased in the second and third week, and the weekly change of blood sugar was also decreased in the 5th week. Dietary Hamcho intake showed 41.2% of hypoglycemic effect in diabetics rats. Levels of total lipid and triglycerides of DH group were lower than those of DC group. Hepatic GR activity of DH group was higher than those of other groups. However, renal GR activity was lower than those of other groups. Hepatic G6Pase activity was significantly high in DH group and reduced by Hamcho powder supplementation. GST was reduced by Hancho diet in diabetic rats. In conclusion, Hamcho supplementation decreased serum lipid and glucose concentration in STZ-induced diabetic rats and this effects of Hamcho might exert antidiabetic effect of Hamcho powder diet.

침버섯 인공재배 생육 특성과 수확 최적 시기

김영,정보미,위안진,박화식,방미애,박대훈,서정욱,오득실 한국버섯학회 2015 한국버섯학회지 Vol.13 No.2

침버섯 자실체는 원기형성일로부터 exp{b2 * ln(t)2 + b1* ln(t)}의 생장곡선을 그리면서 성장하는 것으로 나타났으며 재배후 20일째에는 생장 임계선을 가지는 것으로 나타났다. 또한 생장 임계선이 20일째 나타나지만 재배 14일째부터는 다당류의 감소현상이 나타나는 것으로 확인되었다. 이로 인해 재배후 14~15일(자실체 생중량 150 g)째가 최적 수확시기로 사료된다. 이는 Choi et al. (2011)이 연구하여 발표하였던 침버섯의 균사 생장 패턴과 유사하며 배양시기별 기능성 단백질을 생산하는 양에 차이가 있는 것으로 사료된다. 하지만 Park et al. (2004)는 표고버섯에서 채취 시기별 영양소 차이가 존재하지 않지만 부위별 영양소 차이가 존재한다고 하였다. 침버섯은 시기별 생장하는 부위가 다르기에 시기에 따른 기능성 단백질 차이가 아닌 세부 부위별 유효성분 분포 차이로 본 연구와 같은 결과가 도출되었을 가능성도 배제할 수 없다고 사료된다. Myung-Hee et al. (2012)는 시판 되는 버섯의 부위 중 자실체에 주로 유효성분 이 분포한다고 하였다. 따라서 침버섯의 최적 수확시기는 버섯의 자실체에 해당하는 바늘의 생장이 거의 정지되는 시점이기에 성장 시기별 기능성 물질의 생산량의 차이인지 부위별 유효성분 분포 차이인지를 규명할 추가적인 연구가 필요하다. Mycoleptodonoides aitchisonii has been used a culinary material and traditional medicine for a long time in worldwide and recently the researches to find biological effects have been increased such as dopamine activation, preventive effect against phytopathogens, inhibitive effect against erythrocyte coagulation, anti-oxidative effect, anti-tumorigenic effect, etc. However it is hard to cultivate Mycoleptodonoides aitchisonii it is impossible to be mass-produced and in order to solve the problem in this study we found the appropriate cultivation period and the harvest point for it. For life cycle (from primordium formation to harvest) the morphology, weight, and quantity of polysaccharide of Mycoleptodonoides aitchisonii were measured using with 5 bodies per a day and it could be divided for 4 stages; primordium formation, growth, needle maturation, and aging. And then from the results the Zeide nonlinear growth curve could be gotten. At 13th day after cultivation there is the relation between the change of media weight and body weight and at 14th day after cultivation the rate of polysaccharide in the body was 11 %. However in the case of O2 insufficient supply the malformation of them was observed.

다시마 분말이 당뇨 유발쥐의 혈당과 지질농도 및 항산화 효소계에 미치는 영향

조영자,방미애 한국식생활문화학회 2004 韓國食生活文化學會誌 Vol.19 No.4

The purpose of this study was to investigate the effect of dietary sea tangle in diabetic rats treated with streptozotocin(STZ). Four groups of rats (Sprague-Dawley male rats, 180-200g) were normal rats fed control diet(C), diabetic rats fed control diet(CD), normal rats fed sea tangl diet(T), and diabetic rats fed sea tangle diet(TD). Diabetes was induced by single injection of streptozotocin(60㎎/㎏ B.W.). High density lipoprotein(HDL) of T and TD group were higher than other groups(C and T groups). And the weekly change of blood sugar was decreased in the 3th and 4th weeks. But serum triglyceride (TG) of diabetic rats fed sea tangle diet(TD) was lower than diabetic rats fed control diet(CD). Activity of hepatic microsomal Glucose 6-phosphatase(G6Pase) was significantly increased CD and TD groups higher than C and T groups. Hepateic glutathione S-transferase(GST) of T, CD and TD groups were significantly lower than C group(p〈0.05), glutathione peroxidase (GPX) of T and TD groups were significantly higher than C and CD groups(p〈0.05).

Bacillus subtilis BC-P1 균주를 이용하여 제조한 청국장의 발효 및 품질 특성분석

박성용,방미애,오병준,박정훈,송원섭,최경민,정의수,부희옥,조승식,Park, Sung-Yong,Bang, Mi-Ae,Oh, Boung-Jun,Park, Jeong-Hoon,Song, Won-Seob,Choi, Kyung-Min,Choung, Eui-Su,Boo, Hee-Ock,Cho, Seung-Sik 한국미생물학회 2013 미생물학회지 Vol.49 No.3

본 연구진은 청국장에서 수종의 미생물을 분리하였으며, 이중 소화 효소능이 뛰어난 균주를 선별하였으며, 유전학적 분석결과를 통해 본 균주가 Bacillus 속 균주임을 확인하고 Bacillus subtilis BC-P1이라 명명하였다. Bacillus subtilis BC-P1을 starter로 사용하여 청국장을 제조하였으며, protease, xylanase, chitinase activity, 혈전 분해능, 영양성분 분석 및 아미노산 분석을 통하여 B. subtilis BC-P1를 starter로 활용한 청국장 제품의 개선 가능성을 타진하였다. Bacillus subtilis BC-P1를 starter로 제조한 청국장은 기존 제품에 비해 우수한 소화효소 생성능을 보였으며, 특히 혈전분해효소 생성능이 우수함을 확인하였고, 대조군에 비해 증가된 환원당 및 총 아미노산 함량을 확인 할 수 있었다. 따라서 본 균주를 starter로 하여 청국장을 제조할 경우 기능성분, 생리활성의 증가, 품질 향상을 기대할 수 있을 것으로 판단되며, 향후 대규모 생산시 균주, 제조 조건에 관한 연구가 필요할 것으로 사료된다. The object of this study was to improve the quality of Cheonggukjang with new starter, Bacillus subtilis BC-P1. Twenty strains were isolated from the commercial cheonggukjang and 1 Bacillus strain (BC-P1) with protease activity was selected. The 16S rRNA gene sequence revealed that the BC-P1 was closely related to B. subtilis with 99% homology. The quality characteristics of chunggukjang fermented with B. subtilis BC-P1, Bacillus nato (PC) and commercial chunggukjang (NC) were investigated. The characteristics of fermentation were determined by protease, lipase, xylanase, chitinase, and fibrinolytic activities, reducing sugar, nutrient composition and amino acid contents of cheonggukjang sample. Cheonggukjang fermented with B. subtilis BC-P1 showed the strongest fibrinolytic, xylanase, and chitinase activities. Reducung sugar contents of Cheonggukjang samples were $30.16{\pm}2.11$ mg/g (NC), $28.56{\pm}1.52$ mg/g (PC), $32.39{\pm}1.87$ mg/g (BC-P1). And their total amino acid contents were 338.99 mg% (NC), 445.19 mg% (PC), 741.35 mg% (BC-P1). These results suggested that B. subtilis BC-P1 was suitable to be used as a starter to enhance the quality and effects of cheonggukjang.

식이 중 인동초를 섭취한 당뇨유발 흰쥐의 혈당 및 지질대사

조영자,김현아,방미애 목포대학교 생활과학연구소 2000 생활과학논집 Vol.3 No.-

The purpose of this study was to invesgate the effect of L. japonica tumbo(P) on blood glucose level and lipid metabolism in streptozotocin(STZ) induced diabetic rats. Male Sprague-Dawley rats weighing(180~200g)were divided into four groups: control(C), STZ-control(CD), and two experimental groups(P and PD). Diabetes mellitus were induced by single injection of the streptozotocin at tue dose of 60mg/kg B.W. The experimental groups were fed a AIN-76 recomendation diet mixed with the P powder(5%/kg diet). The diet and water were intake determined everyday. The blood glucose level was determined everyweek, and the serum cholesterol was determined every two weeks. After 5 weeks, the rats sacrificed and the blood glucose, serum cholesterol. triglyceride, HDL-cholesterol concentrations were determined. LDL-cholesterol concentrations was calculated by eqation. Diabetic rats showed the lower weight gain compared to the normal rats. The serum glucose level of PD was not lower than C group. However, the serum cholesterol, triglyceride and HDL-cholesterol level of CDI and PD group were heigher than C group. Diabetic rats showed that the hepartic glutathione reductase(GR) was sighificantly heigher than C group, but malondialdehyde level was significantly decreased. P supplymentation was reduced the hepartic malondialdehyde(MDA) level. The glutathione reductase(GR) and HDL-cholestrol level were increased in the diabetic experimental groups compared C group. In comculusion, showing the possibility of preventive and therpeutic use of the wild edible plant(Indong- Cho) to the diabetes mellitus.

자색고구마가 사염화탄소 투여에 의한 흰쥐의 간손상 보호에 미치는 영향

조영자,김현아,방미애,오용비,정병춘,문윤호,정우진 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.3

The purpose of this study was to investigate the effects of dietary purple sweet potato(lpomoea batatas) powder on serum lipid levels and antioxidative enzymes in normal and pretective effect on hepatotoxicity rats induced by carbon tetrachlolide. Four groups of rats (3-week-old inbred Sprague-Dawley male rats) were normal rats fed control diet(C)_(1) induced hepatotoxicity rats fed control diet(EC)_(1) normal rats fed purple sweet potato diet(P), and induced hepatotoxicity rats fed purple potato sweet diet(EP). Rats were induced by single injection of 50% carbon tetrachloide(0.1 mL/100 g B.W., i.p.). The rats were fed ad libitum each of the experimental diet for 5 weeks. After 5 weeks the rats were sacrificed and activities of antioxidant enzymes and lipid peroxidation products were determined in their liver homogenates. But serum concentrations of lipid was not significant in all groups. Serum alanine aminotransferase(ALT/GPT) and aspartate aminotransferase(ALT/GPT)of the EC and EP groups were heigher than the C and P groups. The hepatic glucose 6-phosphatase(G9Pase) activity of the group fed purple potato diet(P) was lower than the other groups(p<0.05). However, The glutathione peroxidase(GPx) activities was not statistically different between the groups. Renal glutathione S-transferase(GST) activity of the EC and EP groups were lower than the C and P groups(p<0.05). In conclusion, these results suggest that purple sweet potato is believed to be possible protective effect on hepatotoxicity rats induced by carbon tetrachlolide.

Streptozotocin-유발 당뇨쥐에서 다시마 추출물 첨가식이의 항당뇨 및 항산화 효과

조영자(Cho Young-Ja),방미애(Bang Mi-Ae) 韓國營養學會 2004 Journal of Nutrition and Health Vol.37 No.1

발열자 능동 감지 시스템을 위한 딥러닝 알고리즘 개발

김용중(YongJoong Kim),최병상(ByungSang Choi),이기섭(KiSeop Lee),정경권(KyungKwon Jung) 한국통신학회 2021 한국통신학회 학술대회논문집 Vol.2021 No.11

This paper propose a deep learning algorithm for an active detection system for persons with fever. The proposed algorithms extracts a sufficient temporal artery region similar to the central temperature of the human body. The proposed algorithm is in development. The developed algorithm will be adopted to Active Detection System. The system to be developed will be very useful in preventing the spread of infection by quickly finding a fever in COVID-19, which is currently prevalent around the world, and similar respiratory-related diseases in the future.

건강기능식품 원료로서 나주 배박 추출물의 지표성분 분석법 벨리데이션

조은정(Eun-Jung Cho),방미애(Mi-Ae Bang),조승식(Seung-Sik Cho) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.11

본 연구진은 배박 추출물이 지방세포 대사를 저해함을 확인하였으며, 배박을 건강기능식품 개별 인정형 소재로 개발하고자 하였다. 소재 개발에 앞서 배박 분석방법을 확립하고자 하였으며, 본 연구에서는 배박에서 확인된 성분인 caffeic acid 및 chlorogenic acid를 HPLC/UV system을 이용해 분석방법을 최적화하였다. 분석법이 타당한지를 검토하기 위하여 분석법 벨리데이션을 수행하였으며, 직선성, 일간 일내 정확성, 정밀성 검출한계 및 검량한계를 확인하였다. 본 시험분석법으로 배박 추출물 분석 시 지표물질이 다른 물질의 간섭 없이 안정되게 분석되는 것을 확인하였다. 따라서 본 분석방법은 배박 추출물 기능성 원료 표준화를 위한 시험법으로 적합한 것으로 판단되었다. 본 시험법에 따라 에탄올 투입량에 따른 배박 추출물 내의 caffeic acid 및 chlorogenic acid의 함량을 분석하였으며, 에탄올 투입에 따른 대상 성분의 추출율을 조절할 수 있음을 보여주었다. 따라서 본 연구를 통하여 확립된 분석법이 배박 추출물 개별 인정형 건강기능식품 기능성 원료 개발을 위한 기초 자료로 활용될 것으로 사료된다. This study was conducted to establish an HPLC analysis method for determination of marker compounds as part of materials standardization for development of health functional food materials from pear pomace. The quantitative determination method of caffeic acid and chlorogenic acid as marker compounds of pear pomace extract (PPE) was optimized by HPLC analysis using a C18 column (5×250 mm, 5 μm) with a 0.2% elution gradient of acetic acid and methanol as the mobile phase at a flow rate of 0.8 mL/min and detection wavelength of 330 nm. The HPLC/UV method was applied successfully to the quantification of marker compounds in PPE after validation of the method with linearity, accuracy, and precision. The method showed high linearity of the calibration curve with a coefficient of correlation (R<SUP>2</SUP>) of 0.9999, and limit of detection and limit of quantification were 1.14 μg/mL (caffeic acid) and 1.61 μg/mL (chlorogenic acid) as well as 4.9 μg/mL (caffeic acid) and 4.9 μg/mL (chlorogenic acid), respectively. Relative standard deviation values from intra- and inter-day precision were less than 3.1% (caffeic acid) and 4.0% (chlorogenic acid), respectively. Recovery rates of caffeic acid and chlorogenic acid at 12.5, 25, and 50 μg/mL were 93.66∼106.32% and 97.33∼105.68%, respectively. An optimized method for extraction of caffeic acid and chlorogenic acid in PPE was established through diverse extraction conditions, and the validation indicated that the method is very useful for evaluation of marker compounds in PPE to develop a health functional food material.