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백서와 기니픽의 대뇌피질에서 Opioid Kappa 수용체의 특성에 관한 연구
김기원(Kee-Won Kim),노혜원(Hye-Won Rho),김형일(Hyoung-Il Kim),은재순(Jae-Soon Eun),소수미(Soo-Mi Soh),조규박(Kyu-Park Cho) 대한약리학회 1994 대한약리학잡지 Vol.30 No.2
In this study, we tested the influences of several κ opioid ligands on the [<sup>3</sup>H]diprenorphine binding in rat and guinea pig cortex membrane preparations. Using paradigm to block μ and δ opioid receptors with DAMGO(1μM) and DPDPE(1μM), [<sup>3</sup>H]diprenorphine labeled κ sites. Competition analysis in both rat and guinea pig cortex has shown a single population of [<sup>3</sup>H]diprenorphine binding site with different Kd values, respectively. There is a significant difference in Ki values of (-) WIN44441 and (+)WIN44441 in both rat and guinea pig cortex. Bremazocine, (-)ethylketocyclazocine, (-)cyclazocine, nor-binaltorphimine effectively inhibited the [<sup>3</sup>H]diprenorphine binding with different Ki values in rat and guinea pig cortex. U-69,593, U-50,488H and dynorphine-A (1-8) did not inhibit the [<sup>3</sup>H]diprenorphine binding in rat but in guinea pig cortex. Nor-binaltorphimine was a ligand discriminate the κ<sub>1</sub>, and κ<sub>2</sub> receptor most effectively. We, also, examined the influence of Na ion and GTPγS, a nonhydrolyzable guanine nucleotide analog, on the inhibition of [<sup>3</sup>H]diprenorphine binding by diprenorphine, (-)ethyl-ketocyclazocine, U-69,593 and bremazocine. By the replacement of NaCl with N-methy-D-glucamine or addition of GTPγS, Ki values of diprenorpnine were not changed and that of ethylketocyclazocine were changed significantly in both rat and guinea pig cortex. The Ki value of bremazocine was decreased by removal of Na ion, and increased by GTPγS, however, was not changed by any one of either. These results suggest that there are 2 kinds of subtypes of κ opioid receptor, κ<sub>1</sub>, and κ<sub>2</sub>, showing different Ki values for various κ opioid ligands, also, bremazocine possess the antagonistic property at κ<sub>2</sub> site which is dominant subtype of K receptor in rat cortex.
( Tae Jun Park ),( Keun Soo Ahn ),( Yong Hoon Kim ),( Hyungseop Kim ),( Ui Jun Park ),( Hyoung Tae Kim ),( Won Hyun Cho1 ),( Woo Hyun Park ),( Koo Jeong Kang ) 대한간학회 2014 Clinical and Molecular Hepatology(대한간학회지) Vol.20 No.1
Hepatopulmonary syndrome (HPS) is a serious complication of end-stage liver disease, which is characterized by hypoxia, intrapulmonary vascular dilatation, and liver cirrhosis. Liver transplantation (LT) is the only curative treatment modality for patients with HPS. However, morbidity and mortality after LT, especially in cases of severe HPS, remain high. This case report describes a patient with typical findings of an extracardiac pulmonary arteriovenous shunt on contrast-enhanced transesophageal echocardiography (TEE), and clubbing fingers, who had complete correction of HPS by deceased donor LT. The patient was a 16-year-old female who was born with biliary atresia and underwent porto-enterostomy on the 55th day after birth. She had been suffered from progressive liver failure with dyspnea, clubbing fingers, and cyanosis. Preoperative arterial blood gas analysis revealed severe hypoxia (arterial O2 tension of 54.5 mmHg and O2 saturation of 84.2%). Contrast-enhanced TEE revealed an extracardiac right-to-left shunt, which suggested an intrapulmonary arteriovenous shunt. The patient recovered successfully after LT, not only with respect to physical parameters but also for pychosocial activity, including school performance, during the 30-month follow-up period. (Clin Mol Hepatol 2014;20:76-80)
Won-Young Park,Min Soo Kim,Min-Seok Kim,Min-Hee Oh,Su-Young Lee,Sun-Hun Kim,Jin-Hyoung Cho 대한치과교정학회 2019 대한치과교정학회지 Vol.49 No.5
Objective: This study aimed to investigate the effect of pre-applied orthodontic force on the regeneration of periodontal ligament (PDL) tissues and the underlying mechanisms in tooth replantation. Methods: Orthodontic force (50 cN) was applied to the left maxillary first molars of 7-week-old male Sprague– Dawley rats (n = 32); the right maxillary first molars were left untreated to serve as the control group. After 7 days, the first molars on both sides were fully luxated and were immediately replanted in their original sockets. To verify the effects of the pre-applied orthodontic force, we assessed gene expression by using microarray analysis and real-time reverse transcription polymerase chain reaction (RT-PCR), cell proliferation by using proliferating cell nuclear antigen (PCNA) immunofluorescence staining, and morphological changes by using histological analysis. Results: Application of orthodontic force for 7 days led to the proliferation of PDL tissues, as verified on microarray analysis and PCNA staining. Histological analysis after replantation revealed less root resorption, a better arrangement of PDL fibers, and earlier regeneration of periodontal tissues in the experimental group than in the control group. For the key genes involved in periodontal tissue remodeling, including CXCL2, CCL4, CCL7, MMP3, PCNA, OPG, and RUNX2, quantitative RT-PCR confirmed that messenger RNA levels were higher at 1 or 2 weeks in the experimental group. Conclusions: These results suggest that the application of orthodontic force prior to tooth replantation enhanced the proliferation and activities of PDL cells and may lead to higher success rates with fewer complications.
The Kinetic Characteristics of K228G Mutant Horse Liver Alcohol Dehydrogenase
Cho, Sun-Hyoung,Ryu, Ji-Won,Lee, Kang-Man The Pharmaceutical Society of Korea 1999 Archives of Pharmacal Research Vol.22 No.1
The kinetic constants and the reaction mechanism of the K228G mutant horse liver alcohol dehyrogenase isoenzyme E (HLADH-E) were compared to the wild-type enzyme. All the Km and Ki constants of the mutant enzyme for NAD+, ethanol, acetaldehyde and NADH were larger than those of the wild-type enzyme. The dissociation constants for the NADH and $NAD^{+}$ (Kiq and Kia) were greatly increased by 130-and 460-fold, respectively. The product inhibition patterns suggested that the reaction mechanism of the mutant enzyme was changed to Random Bi Bi. These results could attribute to the increase in the dissociation rate of coenzyme with the substitution at Lys-228 residue.
β‐cell mass in people with type 2 diabetes
Cho, Jae‐,Hyoung,Kim, Ji‐,Won,Shin, Jeong‐,Ah,Shin, Juyoung,Yoon, Kun‐,Ho Blackwell Publishing Ltd 2011 Journal of diabetes investigation Vol.2 No.1
<P><B>Abstract</B></P><P>The early occurrence of β‐cell dysfunction has been broadly recognized as a critical determinant of the development and progression of type 2 diabetes. β‐cell dysfunction might be induced by insufficient β‐cell mass, by a dysfunction of the β‐cells, or both. Whether or not β‐cell dysfunction constitutes a cause of reduced β‐cells or vice‐versa currently remains unclear. The results of some studies have measured the loss of β‐cells in type 2 diabetic patients at between 22 and 63% by planimetric measurements. Because β‐cell hypertrophy has been noted in type 2 diabetic patients, the loss of β‐cell number should prove more profound than what has thus far been reported. Furthermore, β‐cell volumes are reduced even in patients with impaired fasting glucose. Such defects in β‐cell mass are associated with increased apoptosis rather than insufficient replication or neogenesis of β‐cells. With these results, although they still require clarification, the peak β‐cell mass might be determined at quite an early stage of life, and then might decline progressively over time as the result of exposure to harmful environmental influences over one’s lifetime. In this review, we have summarized the relevant studies regarding β‐cell mass in patients with type 2 diabetes, and then presented a review of the various causes of β‐cell loss in adults. <B>(J Diabetes Invest, doi: 10.1111/j.2040‐1124.2010.00072.x, 2010)</B></P>
( Hyoung Seob Park ),( Hyung Seop Kim ),( Ji Hyun Sohn ),( Hong Won Shin ),( Yun Kyeong Cho ),( Hyuck Jun Yoon ),( Chang Wook Nam ),( Seung Ho Hur ),( Yoon Nyun Kim ),( Kwon Bae Kim ),( Hee Joon Park 대한내과학회 2010 The Korean Journal of Internal Medicine Vol.25 No.3
Background/Aims: In patients with heart failure (HF), N-terminal prohormone brain natriuretic peptide (NT-ProBNP) is a standard prognostic indicator. In addition, uric acid (UA) was recently established as a prognostic marker for poor outcome in chronic HF. The aim of this study was to determine the combined role of UA and NT-ProBNP as prognostic markers for short-term outcomes of acute heart failure (AHF). Methods: The levels of UA and NT-ProBNP were determined in 193 patients (age, 69±13 years; 76 males) admitted with AHF. Patients were followed for 3 months and evaluated for cardiovascular events, defined as cardiac death and/or readmission for HF. Results: Of the 193 patients, 23 (11.9%) died and 20 (10.4%) were readmitted for HF during the 3-month followup period. Based on univariate analysis, possible predictors of short-term cardiovascular events were high levels of UA and NT-ProBNP, low creatinine clearance, no angiotensin converting enzyme inhibitors or angiotensin receptor blockers, and old age. Multivariate Cox hazard analysis showed that UA levels were independently associated with increased incidence of cardiovascular events (hazard ratio, 1.115; 95% confidence interval, 1.006 to 1.235; p=0.037). Kaplan-Meier survival analysis revealed that patients with UA levels >8.0 mg/dL and NT-ProBNP levels >4,210 pg/mL were at highest risk for cardiac events (p=0.01). Conclusions: The combination of UA and NT-ProBNP levels appears to be more useful than either marker alone as an independent predictor for short-term outcomes in patients with AHF. (Korean J Intern Med 2010; 25:253-259)
Optimization of Correction Factor for Linearization with Tc-99m HM PAO and Tc-99m ECD Brain SPECT
Won, Kyu Chang,Watabe, Hiroshi,Kume, Norihiko,Lee, Hyoung Woo,Hayashida, Kohei,Cho, Ihn Ho,Uyama, Chikao 영남대학교 의과대학 1999 Yeungnam University Journal of Medicine Vol.16 No.2
연구목적: ^(99m)Tc d,l-hexamethylpropyleneamine oxime(HMPAO)와 ^99mTc ethyl cysteinate dimer(ECD)의 뇌세포에 의한 섭취는 뇌혈류량에 비례를 한다. 그러나 뇌혈류량이 아주 높은 경우에는 뇌세포에 의한 섭취가 그 만큼 증가를 하지 않기 때문에 뇌혈류량이 과소평가 될 수 있다. 이를 보안하기 위하여 Lassen이 linearization algorithm을 만들었다. 그러나 이 방정식은 뇌의 상태에 따라 사용되는 알파값으로 표현되는 변수가 변할 수 있다. 저자들은 뇌경색이 있는 10명의 환자를 대상으로 가장 적절한 α값을 구하고자 하였다. 재료 및 방법: 10명의 환자들은 모두 0.1에서 10까지의 알파값을 이용하여 교정한 ^99mTc-HMPAO와 ^(99m)Tc ECD 뇌관류 단일광자방출 단층촬영(single photon emission computed tomography: SPECT) 영상을 구하고, 양전자방출단층촬영술로 뇌혈류영상을 구하였다. 그리고 상호정보의 최대화에 의한 multi-modal volume registration을 이용하는 컴퓨터프로그램으로 양전자방출단층촬영술로 구한 뇌혈류와 SPECT 영상에서 다양한 알파값을 대입하여 Lassen의 linearization algorithm으로 구한 뇌혈류값을 픽셀단위로 서로 비교하였다. 결과: Lassen의 linearization algorithm을 이용하여 구한 ^(99m)TC-HMPAO와 ^99mTC-ECD 뇌관류 SPECT의 국소 뇌혈류량은 알파값이 각각 1.4와 2.1일때 양전자방출전산화단층촬영술로 구한 뇌혈류량과의 상관관계가 가장 높았다. 결론: Lassen의 linearization algorithm을 이용하여 뇌혈류량을 정량화하는 경우에는 ^99mTC-HMPAO의 경우는 1.4, ^(99m)TC-ECD의 경우는 2.1을 사용할 때 뇌혈류량을 가장 잘 반영할 것으로 생각된다.
Won Seok Ju,Jin Hyoung Cho,Sang Young Seo,Ji-Su Kim,Sun-Uk Kim,Soon Ju Park,Kisung Ko,Young-Kug Choo 한국당과학회 2021 한국당과학회 학술대회 Vol.2021 No.01
Mesenchymal stem cells (MSCs), also known as multipotent stromal cells, has a possibility to differentiate into the neurallike cells as well as other type of cells such as osteoblast and adipocyte. Moreover, ganglioside, a sialic acidcontaining glycosphingolipids, plays a significantly critical role in the neuronal differentiation of MSCs. Meanwhile, macrophages (MPs), which secrets many type of proteins including human nucleoside diphosphate kinase A (hNME1) in the body, has been considered to involve closely in the neuronal differentiation of MSCs. Herein, we investigated and analyzed the correlation between the inhibitory effect of MP secretomes and the expression of ganglioside in the neuronal differentiation. Our results show that hNME1 strongly and specifically binds pST8SIA1, which is a ganglioside GD3 synthase, suggesting that hNME1 is a key factor in the inhibitory. Consequently, we produced nanobodies (NBs) named NB-hNME1 that effectively blocked the binding of hNME1, and thereby recovered the expression of ganglioside GD3 and the neuronal differentiation of MSCs.