複合 韓藥劑 淸籬滋坎湯의 抗癌效果 및 作用機轉 糾明

Cho, Kyung-Sam,Kim, Si-Young,Park, Jai-Kyung,Choi, Seung-Hoon,Chung, Se-Young,Yoon, Hwi-Joong 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung Sam Cho¹Si Young Kim¹, Jai Kyung Park²,Seung Hoon Choi³,Se Young Chung⁴, Hwi Joong Yoon¹¹College of Medicine, ²East-West Medical Research Institute, ³College of Oriental Medicine, ⁴College of Pharmacology, Kyung Hee University,Seoul, Korea. The Anti-cancer Effect of Oriental Medicine Chung-Ri-Ja-Gam-Tang in the Mouse with Metastatic Lung Cancer. Proceedings of International Symposium on East-West Medicine, Seoul. 231-243, 1999.-The oriental medicine Chung-Ri-Ja-Gam-Tang is an herbal medicine which has been used for pulmonary diseases and known as having immune stimulatory effects. It has been known effective in lung cancer. So we studied the effects and the mechanisms of this herbal medicine it the mouse with metastatic lung cancer. The metastatic lung cancer of the mouse was produced with melnoma cell line(B16BL/6).5×10□/mouse tumor cells were injected intravenously to the CDF1 mouse via tail vein. The mice were divided 4 groups. The first group was treated with 50mg/kg extract of Chung-Ri-Ja-Gam-Tang per oral for 10 days after cancer cell injection, second group treated with saline after cancer cell injection, the third group with medicine without cancer cell, and the last group with saline only. After 10 days treatments some of the mice were scarificed and the lung and spleen was removed. The survival duration, weight change, the number of metastatic cancer nodule of the lung, the NK cell activity, the capacity of cytokines(INF-γ,INF-α,IL-2) production and the proliferation activities of mouse lymphocytes were measured. The surival times of the group 1 mice were longer and the weight loss was less than the group 2 significantly. The number of the metastatic nodule of the lung were decresed in the group 1 than the group 2. The production of INF-γ,was increase in group 2 than group 3 and 4, IL-2 production was increased in group 1 than group 2,3,4 significantly. There was no difference in TNF- α production and proliferation activity of lymphocyte in each group. The NK cell activity was significantly increased in group 1 than group 2,3,4. We conclude that the Chung-Ri-Ja-Gam-Tang has the effect of increasing the NK cell activity of the CDF1 mouse with metastatic lung cancer(B16BL/6). And it is suggested that the increased production of the IL-2 is the mechanisms of enhanced NK cell activity.

敎授 學習過程과 Communication의 理論的 考察

曺正基 新羅大學校 1984 論文集 Vol.17 No.-

The present issue in Modern education is apparently to increase the potentials of studying by students-themselves. To solve this problems, the process of teaching-learning looks very important. According to our viewpoint, teaching-learning could be difined in two-different ways. S.M. Corey difined the teaching as follow;To teach the Individual to show particular action as a response to a situation under a given condition and manipulate the environment with intention that surrounds him to participate in particular action. Also, A.T. Gates, defines "lerning is the progressive acceptance of behavior through experience and traing. Cho Chung-Ki, defines the teaching in his book (Human Management, 1981, p. 100) as follow;the communication is taking of one's own knowledge, thought and attitude by all and is the process that express and transmit his opinion to others. With this concept, teaching-learning process could be regarded as process of Communication. Because the basic function of Education is to transmit the cultural legacies, accumu- lated through history, from the old generations to New generations, I could possibly prove that teaching & instruction would be the process of communi-cation. In other words, the teacher, who is transmitter, sends his message using his text and educational aids. And the student, the receiver, receiver the message and in-terpret these for learning. As a conclusion, the instruction would be possible by the help of Communication. To be effective in Education, the employment of the function and technique of Communication is mostly desired.

바나나 과실 함유탄닌이 소화효소 작용에 미치는 영향

정정한,류충호,조영수 東亞大學校附設遺傳工學硏究所 1997 遺傳工學硏究 Vol.- No.4

바나나 과실을 시중에서 구입하여 미숙과실과 황숙과실로 구별하고 이것을 과육부분과 과피부분으로 나누어서 동결건조 및 열풍건조시켜 실험 시료로서 사용하였다. 탄닌 함량을 분석한 결과 황숙과실 보다도 미숙과실에서 높았으며, 과육보다는 과피에서 열풍건조 보다는 동결건조시킨 시료에서 높은 경향을 나타내었다. 탄닌의 분획은 Sephadex LH-20칼럼을 사용하여 분획하였다. 바나나 과실에서 함유하고 있는 탄닌은 prodelphinidin, procyanidin을 구성단위로 하는 proanthocyanidin류의 축합성 탄닌으로 이러한 탄닌은 소화효소인 trypsin(EC 3. 1. 1. 3), α-amylase(EC 3. 4. 21. 4), lipase(EC 3. 2. 1. 1)에 대하여 in vitro에 있어서 저해작용을 가지고 있는 것이 확인되었으며 또한 탄닌은 중합도가 높을수록 저해율이 높다는 것이 확인되었다. It has been shown that tannins have adverse effects on growth of animals and feed utilization. Tannins are usually classified into hydrolyzable and condensed types but the adverse effects are more marked in condensed tannin in hydrolyzable tannin. Furthermore, the principle condensed tannins found in banana fruits are pro types by the polymerization of flavan-3, 4-diols either alone or in combination with other flavonoids such as catechins. Tannin of the investigated banana(Banana;Musa sapientum LINN)fruits was fractionated into four or five molecular forms, according to the degree of polymerization by chromatography on a column of Sephadex LH-20. The protein-precitating capacity of the fraction noted tannins increased in degree polymerzation. The inhibitory effect of tannins on trypsin(EC 3. 4. 21. 4), α-amylase(EC 3. 2. 1. 1) and lipase(EC 3. 1. 1. 3) activities in vitro also increased with the increased in degree of polymerization.

The Effects of Education on Lead Poisoning Prevention for Parents of Preschool Children

Chung?Min Cho 한국생활환경학회 2014 한국생활환경학회지 Vol.21 No.4

Purpose: The purpose of this study was to evaluate the effect of preventive education of lead poisoning for parents with preschool?aged children. Method: The respondents were 30 parents of preschool children. Data were collected before and after education. Parents in the study were educated on lead poisoning prevention for 3 weeks using in developed brochure. Data were analyzed with SPSS (version 21.0), and included descriptive statistics, paired-t test. Results: In this study, lead poisoning prevention education improved parental knowledge(t = -6.49, p = .000) and parents’ preventive health behaviors(t = -2.47, p = .019). Conclusion: Based on the findings this study , it is imperative that health care providers continue to educate parents about primary prevention of lead poisoning.

Three-Dimensional Porous Collagen/Chitosan Complex Sponge for Tissue Engineering

Kim, Sung Eun,Cho, Yong Woo,Kang, Eun Jung,Kwon, Ick Chan,Lee, Eunhee Bae,Kim, Jung Hyun,Chung, Hesson,Jeong, Seo Young The Korean Fiber Society 2001 Fibers and polymers Vol.2 No.2

A three-dimensional, porous collagen/chitosan complex sponge was prepared to closely simulate basic extracellular matrix (ECM) constitutes, collagen and glycosaminoglycan. The complex sponge was prepared by a lyophilization method and had the regular network with highly porous structure, suitable for cell adhesion and growth. The pores were well interconnected, and their distribution was fairly homogeneous. The complex sponge was crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to increase its boilogical stability and enhance its mechanical properties. The crosslinking medium has a great effect on the inner structure of the sponge. The homogeneous, porous structure of the sponge was remarkably collapsed in an aqueous crosslinking medium. However, the morphology of the sponge remained almost intact in a water/ethanol mixture crosslinking milieu. Mechanical properties of the collagen/chitosan sponge were significantly enhanced by EDC-mediated crosslinking. The potential of the sponge as a scaffold for tissue engineering was investigated using a Chinese hamster ovary cell (CHO-K1) line.

Clinical Significance of Classification of Graves` Disease According to the Characteristics of TSH receptor Antibodies

(Won Bae Kim),(Hyun Kyung Chung),(Young Joo Park),(Do Joon Park),(Hong Kyu Lee),(Bo Youn Cho) 대한내과학회 2001 The Korean Journal of Internal Medicine Vol.16 No.3

N/A Background:It has been widely accepted that the epitope (s) and/or functional characteristics of thyrotropin receptor antibodies (TSHRAb) from Graves` patients are heterogenous among patients. However, the clinical significance of such heterogeneity has not been systematically evaluatedyet. We were to elucidate and find the clinical significance of heterogeneity for TSH receptor antibodies in Graves` disease. Methods:We measured stimulating TSHRAb (TSAb) activities using CHO-hTSHR cells, FRTL-5 cells and chimeric receptor expressing cells (Mc1+2 and Mc2), specific blocking TSHRAb (TSBAb) activities using Mc2 cells and TBII activities using porcine thyroid membrane in 136 patients with untreated hyperthyroid Graves` disease. Results: Based on various TSHRAb activities from each patient, the patients could be categorized into 7 subgroups by cluster analysis; 1) Group 1 (n=41) was characterized by moderate TSAb activities bothin CHO-hTSHR cells and in FRTL-5 cells, typical TSAb epitope, rare blocking antibodies and high TBII activities. 2) Group 2 (n=16) was characterized by the presence of blocking TSHRAb in most patients, albeit the other characteristics were the same as those in Group 1. 3) Group 3 (n=19) patients had low TSAb activities bothin CHO-hTSHR cells and in FRTL-5 cells, seldom had blocking TSHRAb, but they had high TBII activities. 4) Group 4 (n=30) could be categorized as `mild disease` group, as they had low activities in all kinds of TSHRAb assay and had low antim icrosomal antibody activities. 5) Group 5 (n=14) was characterized by moderate TSAb activities with atypical epitope (s), rare blocking TSHRAb and moderate TBII activities. 6) Group 6 (n=10) patients had veryhigh TSAb activities with typical epitopes, seldom blocking TSHRAb and low TBII activities. 7) Group 7 (n=6) was characterized by very high TSAb activities with atypical epitopes and high TBII activities. Pretreatment serum thyroid hormone level was low only in group 4 patients compared to the other 6 groups (p<0.05). The size of goiter was significantly larger in those in group 1 and group 3 (p<0.05) compared to the other 5 groups. The prevalence of clinically significant ophthalmopathy was higher in group 2 patients than the other 6 groups (50%vs.27.5%,p=0.06). Among 6 kinds of TSHRAb activities, only the blocking TSHRAb activity was significantly associated with the presence of ophthalmopathy in multivariate analysis. Conclusion:These results suggest that the differences inepitopes for TSAb or the presence of blocking TSHRAb is not a major factor in determining the degree of thyrotoxicosis in Graves` disease. Although the pathogenic mechanism is not clearyet, we suggest that patients with ophthalmopathy have different TSHRAb repertoire from those without ophthalmopathy in Graves` disease.

단크론항체를 이용한 소 로타바이러스 감염증 치료시험

최해연 ( Hae Yean Choi ),박재명 ( Jae Myong Park ),이은정 ( Un Jeong Lee ),조우영 ( Woo Young Cho ),이종인 ( Jong In Lee ),조부제 ( Bu Jae Cho ),정운선 ( Un Sun Chung ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.21 No.2

To study the efficacy monoclonal antibody(MAb) against bovine rotavirus(BCV) in treatment of calf diarrhea, the MAb was fed to 166 calves with diarrhea from Chung-buk area. The results were summarized as follows. 1. Among the 1,049 calves investigated, 166(16%) calves were infected with BCV. 2. The monthly rate of BCV infection were higher in October to December compared with other months of the year. 3. Among the 166 calves with diarrhea, 137(83%) calves were recovered. 4. Young calves within 7-day-old were more effective in treatment than other ages and the rate of treatment was 86%. 5. Most effective period for treatment of rotavirus was at the first stage of infection.

식육중 테트라사이클린계 항생물질 잔류조사

박재명 ( Jae Myoung Park ),최해연 ( Hae Yean Choi ),이은정 ( Eun Jeong Lee ),조우영 ( Woo Young Cho ),조부제 ( Boo Je Cho ),정운선 ( Un Sun Chung ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.2

In an effort to improve the quality of meat and to monitor farms, residual tetracyclines in local beef and pork produced in the province of North Chung-cheong were determined by a EEC 4-plate, Charm Ⅱ and HPLC, respectively. The results were summarized as follows; 1. Of the 547 samples, 4 beef and 13 pork samples were judged to be positive by EEC 4 -plate method. 2. Detection rates of tetracyclines by type 1(+ + - -) and type 2(+ + + or + + ± -) micro-bial growth inhibition in EEC 4-plate method were 100% and 71%, respectively. 3. Of 17 positive samples, 6 were positive for tetracyclines, 4 were positive for tetracyclines and sulfonamides, 1 was positive for sulfonamides, and 2 were positive for others by Charm Ⅱ test. 4. The best eluents were 0.01M methanolic oxalic acid, and the ideal temperature for stable concentration was 40℃ as optimal HPLC analytical conditions for the detection of tetracy-clines. 5. Of the 10 positive samples for tetracyclines by Charm Ⅱ test, tetracyclines were confirmed in 2 beef and 6 pork samples, using HPLC, at levels ranging from 3.64~4.22 ppm and 0.2~1.20 ppm, respectively.

下水 處理場의 最適處理工程에 關한 實驗的 硏究

조정석,한양수 대구대학교 과학기술연구소 1995 科學技術硏究 Vol.2 No.1

The growth of population and industrialzation has been increasing the discharge of wastewater and requiring more intensive eater treatment processes. This study was carried out to determine the optimal design conditions of a sewage treatmentplant in the small city of An-jung. Also, we set out to find the operation guide data pertaining to the activated sludge process required by a city of this size. The biological reactors were operated with hydraulic retention time of 4hrs, 6hrs, 8hrs, 10hrs, and 12hrs respectively, using a mixture of raw wastewater. From the results of the experiments, we found that the removal efficiency increased as the F/M ratio decreased. From this conclusion we found that we could get the BOD removal eficiency above 90% if the F/M ratio was 0.13 to 0.27 KgBOD/Kg MLSSday.

Box-Jenkins法을 利用한 河川水質의 時系列 豫測

趙廷錫,韓陽洙,金喜洛 大邱大學校 科學技術硏究所 1997 科學技術硏究 Vol.4 No.2

This study was carried out to develop the stochastic stream water quality model for the intaking station of Taejoun City waterworks in the Keum River system. The monthly water dissolved oxygen with periodicity and trend were forcasted by multiplicative ARIMA models and then the applicability of the models was tested based on 13years of the historical monthly water quality data at Taejoun City intaking site. The parameter estimation was made with the monthly observed data. The last one year data was used to compare the forcasted water quality by ARIMA model with the observed. The models are ARIMA(2.0.2)x(2.1.1)_(12) for dissolved oxygen. The forecasting results showed a good agreement with the observed. It is implying the applicability of multiplicative ARIMA model for forecasting monthly water quality at the Taechoung site.