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박재명 ( Jae Myoung Park ),최해연 ( Hae Yean Choi ),이은정 ( Eun Jeong Lee ),조우영 ( Woo Young Cho ),조부제 ( Boo Je Cho ),정운선 ( Un Sun Chung ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.2
In an effort to improve the quality of meat and to monitor farms, residual tetracyclines in local beef and pork produced in the province of North Chung-cheong were determined by a EEC 4-plate, Charm Ⅱ and HPLC, respectively. The results were summarized as follows; 1. Of the 547 samples, 4 beef and 13 pork samples were judged to be positive by EEC 4 -plate method. 2. Detection rates of tetracyclines by type 1(+ + - -) and type 2(+ + + or + + ± -) micro-bial growth inhibition in EEC 4-plate method were 100% and 71%, respectively. 3. Of 17 positive samples, 6 were positive for tetracyclines, 4 were positive for tetracyclines and sulfonamides, 1 was positive for sulfonamides, and 2 were positive for others by Charm Ⅱ test. 4. The best eluents were 0.01M methanolic oxalic acid, and the ideal temperature for stable concentration was 40℃ as optimal HPLC analytical conditions for the detection of tetracy-clines. 5. Of the 10 positive samples for tetracyclines by Charm Ⅱ test, tetracyclines were confirmed in 2 beef and 6 pork samples, using HPLC, at levels ranging from 3.64~4.22 ppm and 0.2~1.20 ppm, respectively.