Treadmill 보행시의 보행속도, 경사도 및 체중에 따른 산소섭취량 예측식에 관한 통계학적 연구

백정민,정규철 중앙대학교 의과대학 의과학연구소 1985 中央醫大誌 Vol.10 No.1

The oxygen uptake in treadmill walking at speeds of 2, 4. 6 and 8 km/hr on the slopes from 0(level) to, 25% of gradient was measured by means of the Douglas bag method on 74 male college students. Correlation coefficients and multiple regression formulae Of VO^2(ml/min) and VO^2/body weight(ml/kg/min) on multivariables such as speed, square of speed, gradient and body weight were calculated. Regression formulae for prediction of the gross oxygen uptake(formulae 1, 3, 4, 5, 6 and 7) in treadmill walking were all highly significant with correlation coefficients ranging from 0.84150 to 0.89227, and standard errors of the estimates ranged from 397.3 ml/min to 475.8 ml/min. Regression formulae for prediction of the oxygen uptake per unit body weight(formulae 2, 8, 9. 10, 11 and 12) were also all significant with correlation coefficients ranging from 0.84201 to 0.90913, and their standard errors of the estimates ranged from 6.6 ml/kg/min to 8.6 ml/kg/min. It was found through regression analysis and means of the differences between the predicted and the actually measured oxygen uptake, that the following two formulae (2) and (3) were the most valid equations for predicting both oxygen uptake per unit body weight and gross oxygen uptake in treadmill walking: VO_2/wt = (0.057G + 0.26) V^2 + 0.5IG + 1.71............(2) VO_2 = 71.6G + 359.6V - 546.1 ........................(3) Standard errors of the estimates and means of the difference between the predicted and the measured by the formula(2) were S.E. = 29.74 ml/kg/min and 0.46 ml/kg/min and S.E. = 258.4 ml/min and = 23.7ml/min by the formulae (3). In predicting the oxygen uptake in treadmill walking, equations expressed as a function of speed or squire of the speed and gradient were all proved to be valid, whether it was gross oxygen uptake or per unit body weight, The mean values of measured oxygen uptake for the Korean male college students were larger than those of the predicted values calculated by the formulae suggested by some foreign researchers. This discrepancies may arise, in part, from the racial difference in aerobic work capacity.

The Junction of Language and Culture : The Significance of Teaching Culturally Loaded English Words

Yook,Cheong-Min 신영어영문학회 2011 신영어영문학 Vol.49 No.-

Yook, Cheong-Min. The Junction of Language and Culture: The Significance of Teaching Culturally Loaded English Words. The New Studies of English Language & Literature 49 (2011): 253-267. This paper highlights the significance of teaching culturally loaded English words that are interpreted differently by people from different cultures. Intended to be a general discussion, the paper explores pedagogical issues related to the acquisition of culturally loaded English words in the EFL context of Korea. It first discusses what culturally loaded words are and why they should receive our attention. It then shows the scarcity of theoretical and empirical research on culturally loaded English words and how to teach them in the EFL context. The paper finally makes several suggestions for teaching culturally loaded English words in the Korean EFL context, including a suggestion to make a list of culturally loaded words and provide relevant cultural information in the textbook. (Chungnam National University)

Simultaneous Determination of Amphetamine-Type Stimulants and Cannabinoids in Fingernails by Gas Chromatography-Mass Spectrometry

Kim, Jin-Young,Cheong, Jae-Chul,Kim, Min-Kyoung,Lee, Jae-Il,In, Moon-Kyo 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.6

A gas chromatography-mass spectrometric (GC-MS) method was developed and validated for the simultaneous detection and quantification of four amphetamine-type stimulants (amphetamine (AP), methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA) and 3,4-methylenedioxymethamphetamine (MDMA)) and two cannabinoids (${\Delta}^9$-tetrahydrocannabinol (${\Delta}^9$-THC) and 11-nor-${\Delta}^9$-tetrahydrocannabinol-9-carboxylic acid (THCCOOH)) in fingernails. Fingernail clippings (30 mg) were washed with distilled water and methanol, and then incubated in 1.0 M sodium hydroxide at $95^{\circ}C$ for 30 min. The compounds of interest were isolated by liquidliquid extraction followed by derivatization with N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) at $70^{\circ}$ for 15 min. The derivatized compounds were analyzed by GC-MS in the selective ion monitoring (SIM) mode. The linear ranges were 0.1-15.0 ng/mg for AP, 0.2-15.0 ng/mg for MDA, ${\Delta}^9$-THC and THCCOOH, and 0.2-30.0 ng/mg for MA and MDMA, with good correlation coefficients ($r^2$ < 0.9991). The intra-day, inter-day, and inter-person precisions were within 10.6%, 6.3%, and 5.3%, respectively. The intra-day, inter-day and inter-person accuracies were between -6.1 and 5.0%, -6.2 and 5.7%, and -6.4 and 5.6%, respectively. The limits of detection (LODs) and quantification (LOQs) for each compound were lower than 0.056 and 0.2 ng/mg, respectively. The recoveries were in the range of 74.0-94.8%. Positive GC-MS results were obtained from specimens of nine suspected MA or cannabis abusers. The concentration ranges of MA, AP, and THCCOOH were 0.10-1.41, 0.12-2.64, and 0.20 ng/mg, respectively. Based on these results, the method proved to be effective for the simultaneous qualification and quantification of amphetamine-type stimulants and cannabinoids in fingernails.

Cellulase 에 의한 목재당화에 (木材糖化) 관한 연구 - (Ⅰ) 기질 (基質) 처리의 효과 -

정대성,민두식 ( Tae Seong Cheong,Du Sik Min ) 한국산림과학회 1978 한국산림과학회지 Vol.38 No.1

In this study, enzymatic hydrolysis of the holocellulose from Alnus hirsuta (Spach) Rupr. (8-14 yr`s) was investigated using crude cellulase preparations of Trichoderma viride Pers. ex. Fr. SANK 16374. And conducted on the optimum condition of the treated substrate for saccharification. A strain of Trichoderma viride Pers. ex. Fr. SANK 16374 was found to be highly efficient for the cellulase productivity, especially in the submerged culture process. The culture medium used in this experiment was prepared from an extract of wheat bran consisting also of KH₂ PO₄ 10, (NH₄)₂ SO₄ 3, NaNO₃ 3, and MgSO₄7H₂O 0.5g/1. Cellulose powder (Toyo filter paper, 60 mesh) was found to be an importent factar for inducing the cellulase formation. And the cellulase produced in the culture fluid was salted out quantitatively by the use of ammonium sulfate (Fig. 1) Reducing sugar was determined by the Dinitrosalicylic acid (DNS) method, using reagents prepared according to the method of Sumner (1925). The results obtained were summerized as follows; 1. The method of delignification were treated by the Peracetic acid (PA) method, according to the method of Toyama (1970). The yield of holocellulose were decreased in accordance with increasing concentration of Peracetic acid solution; delignification of Alnus hirsuta Rupr. with 20% Peracetic acid was satisfied for 48 hours and 40%∼60% peracetic acid was satisfied for 24 hrs: 2. The substrate (holocellulose) was changed easely into fine powder with enzymatic hydrolysis and cellulase exhibits optimum activity on the reducing sugar formation from substrate at the range of 60-100 mesh. 3. The reducing sugar formation increased in accordance with increasing dry temperature on holocellulose substrate was found to be 190±5℃. 4. The optimal heat treated time of holocellulose substrate was found to be 45 min. for the reducing sugar formation showed the best products. The reducing sugar formation did not show statisticaly significent diflerences at 5% levels by heat treated time for 45 min. and 60 min.

Simultaneous Determination of Amphetamine-Type Stimulants and Cannabinoids in Fingernails by Gas Chromatography-Mass Spectrometry

김진영,Jae Chul Cheong,Min Kyoung Kim,Jae Il Lee,Moon Kyo In 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.6

A gas chromatography-mass spectrometric (GC-MS) method was developed and validated for the simultaneous detection and quantification of four amphetamine-type stimulants (amphetamine (AP), methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA) and 3,4-methylenedioxymethamphetamine (MDMA)) and two cannabinoids (Δ9-tetrahydrocannabinol (Δ9- THC) and 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid (THCCOOH)) in fingernails. Fingernail clippings (30 mg) were washed with distilled water and methanol, and then incubated in 1.0 M sodium hydroxide at 95oC for 30 min. The compounds of interest were isolated by liquidliquid extraction followed by derivatization with N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) at 70oC for 15 min. The derivatized compounds were analyzed by GC-MS in the selective ion monitoring (SIM) mode. The linear ranges were 0.1-15.0 ng/mg for AP, 0.2-15.0 ng/mg for MDA, Δ9-THC and THCCOOH, and 0.2-30.0 ng/mg for MA and MDMA, with good correlation coefficients (r2 > 0.9991). The intra-day, inter-day, and inter-person precisions were within 10.6%, 6.3%, and 5.3%, respectively. The intra-day, inter-day and inter-person accuracies were between -6.1 and 5.0%, -6.2 and 5.7%, and -6.4 and 5.6%, respectively. The limits of detection (LODs) and quantification (LOQs) for each compound were lower than 0.056 and 0.2 ng/mg, respectively. The recoveries were in the range of 74.0-94.8%. Positive GC-MS results were obtained from specimens of nine suspected MA or cannabis abusers. The concentration ranges of MA, AP, and THCCOOH were 0.10-1.41, 0.12-2.64, and 0.20 ng/mg, respectively. Based on these results, the method proved to be effective for the simultaneous qualification and quantification of amphetamine-type stimulants and cannabinoids in fingernails.

農民技術受容에 影響을 주는 要因의 比較分析 : 關係機關과 情報傳達媒體를 中心으로

鄭址雄,崔敏浩,金性洙,徐圭善,李昶植 서울大學校 農科大學 1984 서울대농학연구지 Vol.9 No.2

The purpose of the study was to analyze the variables affecting farmers' adoption process of agricultural innovations in Korea. Specific objectives of the study were to ; (1) review the adoption process and the variables associated with this process, (2) investigate the rate of adoption of selected agricultural innovations in the fields of rice production, animal husbandary and horticulture, (3) analyze communication media and change agencies or institutions which affected the adoption of agricultural innovations, and (4) identify the relations of the farmers' adoption and communication exposure behavior. Data for the study were collected from 393 cluster sampled farmers out of selected 17 rural villages in Korea. Five graduate students and three faculty members of Seoul National University participated in conducting interview with pre-tested questionnairs. The data were analyzed by HP 3,000 computer in the College of Agriculture, Seoul National University. Major statistical techniques used for the study were frequency distribution, chi-square and discriminant analysis. Statistical significance was tested at .05 level. The major findings of the study were as follows : 1. Variables associated with the agricultural adoption process were classified into the following six categories ; (1) perceived characteristics of agricultural innovations, (2) personality and characteristics of adopters, (3) the patterns of communication media or channels, (4) efforts of change agents. (5) priorities in agricultural development policies, and (6) characteristics of social system. 2. Rats of adoption were about 83% in application of silicate fertilizers on rice seed bed, 41% in feeding calf starter known as artificial milk to calves, 56% vinyl mulching practices in vegetable cultivation, and 67% in spreading raw rice straw on paddy land to improve soil conditions. 3. Time lag between awareness and adoption appeared to be about seven months in application of silicate fertilizers on rice seed bed, and about a year in feeding calf starter. 4. Change agent interpersonal communication revealed to be the most important factor affecting farmers' awareness in application of silicate fertilizers on rice seed bed, in feeding calf starter, and in spreading raw rice straw on paddy land, while localite interpersonal communication in vinyl mulching practices in vegetable cultivation. 5. Change agent interpersonal communication appeared to be the most important factor affecting farmers' adoption in application of silicate fertilizers on rice seed bed and in feeding calf-starter, Localite interpersonal communications were more important in vinyl mulching practices of vegetable cultivation and in spreading raw rice straw. Localite interpersonal communications were more important factor at adoption stage than at awarenss stage. 6. Agricultural extension agencies appeared to be the most important factor affecting farmers' adoption in application of silicate fertilizers on rice seed bed, in feeding calf starter, and in vinyl mulching practices. Based on the results of this study, the following are recommended for further development of agricultural extension services and effective adoption process of agricultural innovation. 1. The variables associated with the agricultural innovation decision process should be taken into consideration to improve effectiveness of change agencies or agricultural extension services. 2. Effective use of communication media along with the feedback messages from the change agency as well as client system be considered to increase effectiveness and meaningfulness of both systems. 3. Innovative agricultural research be continually sharpen its focus on the highest priority needs of client system by utilizing feedback from farmers through the communication link of change agents. 4. A long-term educational approach by using various communication media be emphasized, and regular field survey on innovation-decision process will prevent discontinuance of innovations.

中國 唐代小說 속에 반영된 女仙 이미지 : 『太平廣記』를 중심으로

정민경 서강대학교 인문과학연구소 2009 서강인문논총 Vol.0 No.25

Novels of China have been developing under a close relation with religion, especially a special relation with Taoism. So this manuscript pays attention to features of a women Taoist immortals[女仙] appearing with what looks and image in novels of Tang age. To begin with, a general idea of it comes by looking into a women Taoist immortals division, a minor division of a grand series of novels of "Tae Pyeong Gwang Gi[太平廣記]". Though a women Taoist immortals division of "Tae Pyeong Gwang Gi" collects stories from Han Dynasty to early Song Dynasty, an aspect of novels at that time can be looked into sufficiently because most of it was collected and created by people of Tang Dynasty. An image of a women Taoist immortals in novels of Tang age can be divided into two phases after analyzing stories of a women Taoist immortals division of "Tae Pyeong Gwang Gi". One has a looks of a women Taoist immortals of religious dimension still showing a divine nature with looks of goddess, and the other has a looks of a women Taoist immortals already secularized and degraded to the status of a lover of men. But it stresses' a wise mother and good wife' in the image of a women Taoist immortals with a religious divine nature and gives prominence to a sexy and erotic side of a women Taoist immortals by reflecting looks of a gisaeng[妓女], a women Taoist[女道士] and even a women ghost[女鬼,] in the image of a lovely women Taoist immortals. And this image of a women Taoist immortals has changed much due to writers of men and Tang society at that time. This image of a women Taoist immortals shown in the novels has become more secularized in later generations and takes on an aspect overlapping even an image of a temptress in the novels. 중국의 소설은 종교와의 긴밀한 연관성 아래에서 발전해왔으며 그 중 도교와의 관계는 특별하다고 할 수 있다. 본 고에서는 도교 속 여선의 모습이 당대소설 속에서 어떠한 이미지를 가지고 어떠한 양상으로 나타나고 있는지에 주목하였다. 우선『太平廣記』라는 소설총집을 대상으로 그 안의 소분류인 女仙類를 살펴봄으로써 그 대강을 파악해보았다. 『태평광기』의 여선류는 漢나라부터 宋나라 초까지의 이야기들을 수록하고 있지만 그 대부분이 당나라 사람들이 수록하고 창작한 것이어서 당대 사회의 면모를 충분히 살펴볼 수 있다. 『태평광기』여선류 이야기를 분석해 본 결과 당대 소설 속 여선의 이미지는 크게 두 가지 양상으로 나타났는데, 그 중 하나는 女神의 모습을 여전히 간직한 채 신성을 드러내고 있는 종교적 차원의 여선의 모습이었고 또하나는 이미 세속화되어 남성의 연인이나 상대로 전락한 여선의 모습이었다. 그러나 종교적 고결함을 갖춘 여선의 이미지 속에서도 유가 윤리적 '현모양처'의 이미지를 강조하고 있고 사랑스러운 여선의 이미지 속에도 당대 女道士나 妓妹, 심지어는 女鬼의 모습까지 투영시켜 여선의 섹시하고 에로틱한 측면만을 부각시키는 것으로 보아, 당시 남성 위주의 사회 속에서 여선의 이미지가 크게 변화되었음을 살펴볼 수 있었다. 이러한 여선의 이미지를 통해 당대소설 속에 반영된 당시 사회의 일면을 살펴볼 수 있을 뿐만 아니라 도교 문학의 세속화 과정도 동시에 찾아볼 수 있었다. 당대 소설 속에 보이는 이러한 여선의 이미지는 후대로 가면서 더욱더 세속화되어 후대 문언소설이나 백화소설 속에서는 심지어 요부의 이미지와도 겹쳐지는 양상을 띠게 된다.

지역사회와 병원에서 페니실린 내성 폐렴구균 감염의 임상적 특징 및 분자유전학적 성상

정희진,황병연,박정원,김우주,김민자,박승철,이영희 대한감염학회 2001 감염 Vol.33 No.2

Background : During the last three decades, the resistance of S. pneumoniae to penicillin has been rapidly increasing in many parts of the world, especially in Korea. To characterize the clinical features and epidemiology of penicillin-resistant S. pneumoniae (PRSP) infections in the community -and hospital, as well as to investigate the possible spread of resistant clone, we performed the antimicrobial susceptibility tests, pulsed filed gel electrophoresis (PFGE) and penicillin-binding protein (PBP) profile analysis of PRSP isolates. Methods : A total 48 PRSP isolates from patients who visited or were admitted to Korea University Guro hospital during the period form July 1998 to June 1999 were studied. Anitimicrobial susceptibility tests for 48 isolates were performed with microbroth dilution method to determine the minimal inhibitory concentration of 11 antibiotics. 39 isolates and 35 isolates were subjected to PFGE and PBP profile analysis, respectively to investigate the genetic relatedness between PRSP isolates. Results : Pneumonia was most common site of infection in the community and the hospital as 50%. There were no significant differences of clinical fentures and prognosis between community and hospital isolates. But, patients with serious underlying diseases had poor prognosis despite of acquisition site. High level penicillin resistance were observed in 69%, multi-drug resistance were 64.6% of isolates. PFGE showed that 13 of 29 community acquired infection were identical PFGE pattern but not that of 23F Spanish clone. There were various PFGE patterns were observed from community and hospital acquired infection isolates. Some of them were existed in both. PBP profiles showed more diverse, even if in isolates of the same PFGE pattern. Conclusion : In our study, high level penicillin resistance and multi-drug resistance were observed in PRSP clinical isolates. No clinical and prognostic differences were observed between community and hospital acquired infections. Molecular epidemiology study were suggest the there were various genotypes of PRSP within our society. Some of them were observed of the hospital and community. Therefore, there was an evidence of communication of PRSP clones between the community and hospital. (Korean J Infect Dis 33:112~122, 2001)

흰민들레 (Taraxacum coreanum) 추출물이 급성 수은 중독된 생쥐의 간에 미치는 효과

정민주,윤중식,허진,노영복,최영복,김종세,이현화 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.1

본 연구는 급성 수은 독성에 대한 민들레의 효과를 알아보기 위하여 시도되었다. 30 g 내외의 생쥐를 대상으로 대조군, 수은(5mg/kg) 투여군, 수은 투여 후 민들레(3 g/kg)를 구강투여 한 후 다시 24, 48, 72, 96시간, 1주일군으로 세분하여 간장 손상 억제 효과를 알아보기 위하여 생화학적 및 조직학적 실험을 실시하였다. 혈액중 aspartate amiotransferase (AST)와 alanine aminotransferase (ALT)의 수치는 민들레 투여군이 수은 투여군보다 감소되었다. 또한 간조직의 SOD와 catalase 활성도 역시 민들레 투여군이 수은 투여군에 비하여 감소하였으나 통계학적 유의성은 없었다. 간 조직의 광학현미경적 관찰에서 심한 조직괴사가 관찰되지만 민들레 투여군에서는 문맥주위의 약간의 괴사와 심한 호중구 침윤현상이 관찰되었다. 전자현미경적 관찰 결과, 간장에서 수은 투여군은 간세포의 핵이 함입되어 불규칙했으며 미토콘드리아와 조면소포체의 수조가 팽대되고, 리보솜의 탈락이 관찰되었다. 민들레 투여군은 핵이 정상적인 상태로 관찰되었고, 전자밀도가 높은 미토콘드리아가 분포되어 있었으며, 리보솜이 부착된 상태로 층판구조를 형성하는 조면소포체가 관찰되었다. 이상의 연구 결과로 보아 민들레가 수은으로 유발된 간손상을 보호하는 효과가 있는 것으로 사료되며 민들레에 대한 다양한 연구가 필요할 것으로 사료된다. Dandelion has been frequently used as a remedy for women’s disease, inflammatory diseases and disorders of the liver and gallbladder. Dandelion extracts water extract, an herbal medication, may have an effect on the activity of hepatic antioxidant enzymes in diabetic rat. This study aims demonstrate the effect of dandelion extracts, one of the natural chelator, on the biochemical and enzyme activity changes in the mouse liver caused by HgCl₂. Mice approximately 30 gm in weight were grouped into the control, mercury chloride-treated, and the dandelion extractstreated after mercury chloride groups. HgCl₂ (5 mg/kg) and dandelion extracts (3 g/kg) were delivered orally. Serum AST and ALT were measured, enzyme activity of liver were examined by spectrophotometer and ultrastructural alteration of liver were examined by light and electron microscopy. Dandelion extracts were decreased the increase of serum AST and ALT level induced by mercury. The catalase activity was decreased in the dandelion extracts group. The activity of SOD was dereased, but did not show significant differences. Mercury chloride-treated hepatic cell were irregular nucleus, enlarged and reduced number of mitochodria, enlarged rough endoplasmic reticulum, loss of ribosomes. Cells treated with dandelion extracts were similar to those of the control group. In conclusion, dandelion extracts may protect the mercury-induced toxicity on Liver.

임상 검체에서 분리된 vancomycin 내성 장구균에 대한 Quinupristin/dalfopristin(Synercid®)을 포함한 10종 항균제의 시험관내 항균력

정희진,박기호,김동림,김우주,김민자,박승철,신종희 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

목적 : 본 연구에서는 고려대 병원과 전남대 병원에서 임상 검체로부터 분리된 VRE 16균주를 대상으로 하여 장구균 감염의 치료에 흔히 사용되는 항균제인 ampicillin, gentamicin, vancomycin, teicoplanin과 VRE에 대해서도 비교적 항균력이 있는 것으로 알려진 기존의 chloramphenicol, doxycycline, erythromycin, ciprofloxacin, rifampicin, 그리고 다제 내성 그람 양성균의 치료를 위하여 새로이 개발된 quinupristin/dalfopristin을 이용한 시험관내 항균력을 조사하고자 하였다. 방법 : 총 16균주의 VRE faecium을 대상으로 quinupristin/dalfopristin (Synercid^(R))를 포함한 10종 항균제에 대한 최소 발육 억제 농도(MIC)를 한천희석법을 이용하여 측정하였다. 각각의 항균제에 대한 감수성은 NCCLS에서 제시한 기준에 의거하여 해석하였다. 결과 : 1) 5균주의 vanB VRE에 대한 항균제의 MIC_(90)은 ampicillin, erythromycin, gentamicin, cipro- floxacin에서 각각 64㎍/㎖, 512㎍/㎖, 512㎍/㎖, 4㎍/㎖로서 100%에서 내성을 보였고, doxycycline, chloramphenicol의 경우 4㎍/㎖, 4㎍/㎖로서 100%에서 감수성을 보였다. Teicoplanin의 MIC_(90)은 1㎍/㎖로서 모두 감수성을 보였고 quinupristin/dalfopristin에 대해서도 MIC_(90) 0.25㎍/㎖로서 감수성을 보였다. 2) 11균주의 vanA VRE에 대한 항균제들의 MIC 범위는 vanB VRE보다는 다양하였다. MIC_(90)을 보면 amplicillin, erythromycin의 경우 256㎍/㎖, 512㎍/㎖으로 100%에서 내성을 보였응며, gentamicin은 MIC_(90) ≥500㎍/㎖의 고도내성을 보인 경우가 8 균주로서 73%에서 내성을 보였다. Ciprofloxacin은 vanB VRE에서와 마찬가지로 MIC≥32㎍/㎖의 100%의 내성을 보였다. Chloramphenicol의 경우는 MIC_(90)이 16㎍/㎖로서 vanA VRE faecium에 대해서도 감수성이 있는 약제로 남아 있었다. 반면에 doxycycline은 MIC_(90)이 4㎍/㎖로서 시험관내에서는 100% 감수성을 보였고, quinupristin/dalfopristin은 MIC_(90)이 2㎍/㎖로서 내성을 보인 균주는 한 균주도 없었다. 결론 : 국내 임상분리 VRE 16균주는 기존에 사용되어 오던 chloramphenicol, doxycyline등의 항균제에 대하여 시험관내에서 100% 감수성을 보였으며 quinupristin/dalfopristin과 같은 새로운 항균제에 대해서도 중등도 또는 완전 감수성을 보여 향후 VRE에 의한 감염증 치료에 있어 이들 약제의 병합요법등이 고려될 수 있을 것이라 사료된다. Background : Vancomcyin-resistant enterococci (VRE) have become a major nosocomial pathogen in the USA during 1990s. In Korea, VRE have recently emerged as a important nosocomial pathogen and posed therapeutic difficulty. We evaluated the antimicrobial susceptibility of clinical VRE isolates to the antimicrobial agents that are commonly used to treat enterococcal infections and to some alternative drugs and to a newer antibiotic such as quinupristin/dalfopristin(Synercid®). Methods : We evaluated 16 clinical VR-E. faecium (5 vanB VRE, 11 vanA VRE) strains that were isolated from Korea Universtiy and Chonnam University hospital during the last five years (1994-1998). The isolates were identified to the species level by using API 20system. The in vitro susceptibility testing was performed by the agar dilution methods, following the criteria described by the National Committee for Clinical Laboratory Standards (NCCLS). The VRE isolates were tested against antimicrobial agents commonly used to treat enterococcal infections (vancomycin, teicoplanin, ampicillin, gentamicin), potential alternative drugs (chloramphenicol, doxycycline, ciprofloxacin, erythromycin, rifampicin) and a newer antibiotic (quinupristin/dalfopristin). Results : 100% (5/5) of vanB VR-E. faecium were resistant to ampicillin, gentamicin and ciprofloxacm, but 100% (5/5) were susceptible to chloramphenicol, doxycycime, teicoplanin and quinupristin/dalfopristm. 100% (11/11) of vanA VR-E. faecium were resistant to ampicillin, ciprofloxacm, 73% were resistant to gentamicin, and 100% (11/11) were susceptible to chloramphenicol and doxycycline. Quinupristin/dalfopristm showed intermediate or high level susceptibility against all vanA VRE isolates. Rifampin is less susceptible (MIC: 4-16 ㎍/mL) against both vanA and vanB VR-E. faecium. Conclusion : The treatment options for infections caused by VRE seem to be very narrow since a small percentage of those isolates were susceptible to the other antmicrobial agents commonly used to treat these infections. And only a few of the alternative drugs including quinupristin/dalfopristm tested showed good in vitro activity. This study suggested that chloramphenicol, doxycycline and qumuprisitin/dalfopristin would be a good candidate to treat VRE infection, but further studies are necessary to demonstrate the clinical role of these antimicrobial agents.