Recent advances in the use of photochromic dyes for photocontrol in biomedicine

Cheng, Hongbo,Yoon, Juyoung,Tian, He Elsevier 2018 Coordination chemistry reviews Vol.372 No.-

<P><B>Abstract</B></P> <P>Light can play important roles in controlling biochemical processes. Owing to their high spatiotemporal resolution and non-invasive nature, light can be utilized as smart triggers to mediate the biological activities of substances. In this review, we summarize important photochromic dye based photoswitchable substances that have been developed in recent years. Additionally, the basic principles involved in the design of the photochromic biomaterials, and insights into how to expand applications of these light activated agents and the key challenges that need to be considered are discussed.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The main approaches for constructing the photochromic biomaterial are reviewed. </LI> <LI> The general concept and importance of optical control of biomedicine are discussed. </LI> <LI> The opportunities and challenges for photochromic biomaterial are included. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

An Interactive Strategy of Grid and Electric Vehicles Based on Master‑Slaves Game Model

Hongbo Cheng,Tong Luo,Chen Kang,Jianbo Xin,Hua Wan,Feng Pei,Xu Tian 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.1

In order to avoid the adverse efects of the grid caused by disorder large-scale electric vehicles and improve supporting function of energy storage battery on the power grid, interaction model of electric vehicles (EVs) and the power grid is constructed according to a master-slave game method. Then, the utility functions of each participant are established, and the dynamic constraints of the batteries for EVs are established considering the travel demand of users. Then, based on the YALMIP/CPLEX solver, the optimal response strategy of the EVs can be obtained, and the genetic algorithm is used to solve the Nash equilibrium solution of the master-slaves game model. Moreover, the Monte Carlo method is employed to simulate the user travel data and the basic parameters of EVs in a certain area. Based on these data, the optimal time-of-use price in this area is ofered, and the optimal response strategy of EVs under this price is analyzed. Finally, the study of the case show that the proposed game model can make the strategies of both the EVs and the grid, which can restrict the load fuctuation of the grid and improve the beneft of EVs without afecting their travel.

Fe3+-binding transferrin nanovesicles encapsulating sorafenib induce ferroptosis in hepatocellular carcinoma

Youmei Xiao,Zhanxue Xu,Yuan Cheng,Rufan Huang,Yuan Xie,Hsiang‑i Tsai,Hualian Zha,Lifang Xi,Kai Wang,Xiaoli Cheng,Yanfeng Gao,Changhua Zhang,Fang Cheng,Hongbo Chen 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Ferroptosis, iron-dependent cell death, is an established mechanism for cancer suppression, particularly in hepatocellular carcinoma (HCC). Sorafenib (SOR), a frontline drug for the treatment of HCC, induces ferroptosis by inhibiting the Solute Carrier family 7 member 11 (SLC7A11), with inadequate ferroptosis notably contributing to SOR resistance in tumor cells. Methods To further verify the biological targets associated with ferroptosis in HCC, an analysis of the Cancer Genome Atlas (TCGA) database was performed to find a significant co-upregulation of SLC7A11 and transferrin receptor (TFRC), Herein, cell membrane-derived transferrin nanovesicles (TF NVs) coupled with Fe3+ and encapsulated SOR (SOR@TF-Fe3+ NVs) were established to synergistically promote ferroptosis, which promoted the iron transport metabolism by TFRC/TF-Fe3+ and enhanced SOR efficacy by inhibiting the SLC7A11. Results In vivo and in vitro experiments revealed that SOR@TF-Fe3+ NVs predominantly accumulate in the liver, and specifically targeted HCC cells overexpressing TFRC. Various tests demonstrated SOR@TF-Fe3+ NVs accelerated Fe3+ absorption and transformation in HCC cells. Importantly, SOR@TF-Fe3+ NVs were more effective in promoting the accumulation of lipid peroxides (LPO), inhibiting tumor proliferation, and prolonging survival rates in HCC mouse model than SOR and TF- Fe3+ NVs alone. Conclusions The present work provides a promising therapeutic strategy for the targeted treatment of HCC.

OTUD7B controls non-canonical NF-kB activation through deubiquitination of TRAF3

( Hongbo Hu ),( George C Brittain ),( Jae Hoon Chang ),( Nahum Puebla Osorio ),( Jin Jin ),( Anna Zal ),( Yichuan Xiao ),( Xuhong Cheng ),( Mikyoung Chang ),( Yang Xin Fu ),( Tomasz Zal ),( Chengming 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0

The non-canonical NF-κB pathway forms a major arm of NF-κB signalling that mediates important biological functions, including lymphoid organogenesis, B-lymphocyte function, and cell growth and survival. Activation of the non-canonical NF-κB pathway involves degradation of an inhibitory protein, TNF receptor-associated factor 3 (TRAF3), but how this signalling event is controlled is still unknown. Here we have identified the deubiquitinase OTUD7B as a pivotal regulator of the non-canonical NF-κB pathway. OTUD7B deficiency in mice has no appreciable effect on canonical NF-κB activation but causes hyperactivation of non-canonical NF-κB. In response to non-canonical NF-κB stimuli, OTUD7B binds and deubiquitinates TRAF3, thereby inhibiting TRAF3 proteolysis and preventing aberrant non-canonical NF-κB activation. Consequently, the OTUD7Bdeficiency results in B-cell hyper-responsiveness to antigens, lymphoid follicular hyperplasia in the intestinal mucosa, and elevated host-defence ability against an intestinal bacterial pathogen, Citrobacter rodentium. These findings establish OTUD7B as a crucial regulator of signal-inducednon-canonical NF-κB activation and indicate a mechanism of immune regulation that involves OTUD7B-mediated deubiquitination and stabilization of TRAF3.

Outd7b facilitates T cell activation and inflammatory responses by regulating Zap70 ubiquitination

( Hongbo Hu ),( Hui Wang ),( Yichuan Xiao ),( Jin Jin ),( Jae Hoon Chang ),( Qiang Zou ),( Xiaopin Xie ),( Xuhong Cheng ),( Shao Cong Sun ) 영남대학교 약품개발연구소 2016 영남대학교 약품개발연구소 연구업적집 Vol.26 No.-

Signal transduction from the T ceII receptor (TCR) is crucial for T cell-mediated immune responses and, when deregulated, also contributes to the development of autoimmunity. How TCR signaling is regulated is incompletely understood. In this study, we demonstrate a ubiquitin-dependent mechanism in which the deubiquitinase Otud7b has a crucial role in facilitatinq TCR sig­naling. Upon TCR ligation, Olud7b is rapidly recruited to the tyrosine kinase Zap70, a central mediator of TCR-proximal sig­naling. Otud7b deficiency attenuates the activation of Zap70 and its downstream pathways and impairs T cell activation and differentiation, rendering mice refractory to T cell-mediated autoimmune and inflammatory responses. Olud7b facilitated Zap70 activation by deubiquitinating Zap70, thus preventing the association of Zap70 with the negative-regulatory phospha­tases SIs1 and Sts2. These findings establish Otud7b as a positive requlator of TCR-proximal signaling and T cell activation. highlighting the importance of deubiquitination in regulaling Zap70 function.

Virtual Resource Allocation in Virtualized Small Cell Networks with Physical-Layer Network Coding Aided Self-Backhauls

( Yulun Cheng ),( Longxiang Yang ),( Hongbo Zhu ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.8

Virtualized small cell network is a promising architecture which can realize efficient utilization of the network resource. However, conventional full duplex self-backhauls lead to residual self-interference, which limits the network performance. To handle this issue, this paper proposes a virtual resource allocation, in which the residual self-interference is fully exploited by employing a physical-layer network coding (PNC) aided self-backhaul scheme. We formulate the features of PNC as time slot and information rate constraints, and based on that, the virtual resource allocation is formulated as a mixed combinatorial optimization problem. To solve the problem efficiently, it is decomposed into two sub problems, and a two-phase iteration algorithm is developed accordingly. In the algorithm, the first sub problem is approximated and transferred into a convex problem by utilizing the upper bound of the PNC rate constraint. On the basis of that, the convexity of the second sub problem is also proved. Simulation results show the advantages of the proposed scheme over conventional solution in both the profits of self-backhauls and utility of the network resource.

Penta-1,2,3,4,6-O-galloyl-beta-D-glucose induces p53 and inhibits STAT3 in prostate cancer cells in vitro and suppresses prostate xenograft tumor growth in vivo.

Hu, Hongbo,Lee, Hyo-Jeong,Jiang, Cheng,Zhang, Jinhui,Wang, Lei,Zhao, Yan,Xiang, Qiu,Lee, Eun-Ok,Kim, Sung-Hoon,Lü,, Junxuan American Association for Cancer Research, Inc 2008 Molecular Cancer Therapeutics Vol.7 No.9

<P>Penta-1,2,3,4,6-O-galloyl-beta-D-glucose (PGG) is a naturally occurring gallotannin from some Oriental herbs. Several cell culture studies suggested a potential for PGG as a novel agent for the chemoprevention and treatment of cancer. Here, we investigated the cell death signaling mechanisms induced by PGG in human prostate cancer cells of different p53 functional status. We observed the induction of G(1)- and S-phase arrests and caspase-mediated apoptosis in the androgen-dependent human LNCaP cells, which express wild-type p53, and in the androgen-independent, p53-mutant DU145 cells. In LNCaP cells, caspase-mediated apoptosis induction by PGG was associated with and mediated in major part by activation of p53 as established through small interfering RNA knockdown and dominant-negative mutant approaches. Intracellular reactive oxygen species production by PGG was found to be crucial for these molecular and cellular actions. In DU145 cells, which harbor constitutively active signal transducer and activator of transcription 3 (STAT3), caspase-mediated apoptosis induction by PGG was associated with an inhibition of STAT3 Tyr705 phosphorylation and the down-regulation of STAT3 transcriptional targets Bcl-XL and Mcl-1. Overexpression of Bcl-XL or knockdown of its binding partner Bak attenuated apoptosis induction. Furthermore, we provide, for the first time, in vivo data that PGG significantly inhibited DU145 xenograft growth in an athymic nude mouse model in association with an inhibition of pSTAT3. Our data support PGG as a multitargeting agent for chemoprevention and therapy of prostate cancer by activating the p53 tumor suppressor pathway and by inhibiting STAT3 oncogenic signaling.</P>

A novel class of pyranocoumarin anti-androgen receptor signaling compounds.

Guo, Junming,Jiang, Cheng,Wang, Zhe,Lee, Hyo-Jeong,Hu, Hongbo,Malewicz, Barbara,Lee, Hyo-Jung,Lee, Jae-Ho,Baek, Nam-In,Jeong, Jin-Hyun,Kim, Dae-Keun,Kang, Kyung-Sun,Kim, Sung-Hoon,Lu, Junxuan American Association for Cancer Research 2007 Molecular Cancer Therapeutics Vol.6 No.3

<P>Androgen and the androgen receptor (AR)-mediated signaling are crucial for prostate cancer development. Novel agents that can inhibit AR signaling in ligand-dependent and ligand-independent manners are desirable for the chemoprevention of prostate carcinogenesis and for the treatment of advanced prostate cancer. We have shown recently that the pyranocoumarin compound decursin from the herb Angelica gigas possesses potent anti-AR activities distinct from the anti-androgen bicalutamide. Here, we compared the anti-AR activities and the cell cycle arrest and apoptotic effects of decursin and two natural analogues in the androgen-dependent LNCaP human prostate cancer cell culture model to identify structure-activity relationships and mechanisms. Decursin and its isomer decursinol angelate decreased prostate-specific antigen expression with IC(50) of approximately 1 mumol/L. Both inhibited the androgen-stimulated AR nuclear translocation and transactivation, decreased AR protein abundance through proteasomal degradation, and induced G(0/1) arrest and morphologic differentiation. They also induced caspase-mediated apoptosis and reactive oxygen species at higher concentrations. Furthermore, they lacked the agonist activity of bicalutamide in the absence of androgen and were more potent than bicalutamide for suppressing androgen-stimulated cell growth. Decursinol, which does not contain a side chain, lacked the reactive oxygen species induction and apoptotic activities and exerted paradoxically an inhibitory and a stimulatory effect on AR signaling and cell growth. In conclusion, decursin and decursinol angelate are members of a novel class of nonsteroidal compounds that exert a long-lasting inhibition of both ligand-dependent and ligand-independent AR signaling. The side chain is critical for sustaining the anti-AR activities and the growth arrest and apoptotic effects.</P>

The critical time of avian leukosis virus subgroup J-mediated immunosuppression during early stage infection in specific pathogen-free chickens

Feng Wang,Xiaowei Wang,Hongbo Chen,Jianzhu Liu,Ziqiang Cheng 대한수의학회 2011 JOURNAL OF VETERINARY SCIENCE Vol.12 No.3

The critical time of avian leukosis virus subgroup J (ALV-J)-mediated immunosuppression was determined by body weight, relative immune organ weight, histopathology, and presence of group specific antigen and antibodies in specific pathogen-free (SPF) chickens. CD4^+ and CD8^+ cell activity in the spleen, total and differential leukocyte counts in blood, and viral RNA levels in spleen were measured. Significant growth suppression was observed in the two ALV-J-infected groups. A strong immune response by infected groups was present in spleen at 2-weeks-of-age, but after 4-weeks-of-age, the response decreased quickly. The thymus and bursa showed persistent immunosuppression until 4-weeks-of-age. Proliferation of fibroblasts and dendritic cells were observed in immune organs at 4- and 5-weeks-of-age. However, the granulocyte cell number was markedly lower in the infected groups than in the control group. In group 1 (day 1 infection) CD4^+ cells increased during the second week but significantly decreased during the fourth week, while group 2 (day 7 infection) showed the opposite effect. Viral RNA increased significantly by the fourth week. These data identify 3∼4 weeks post-infection as the key time at which the ALV-J virus exerts its immunosuppressive effects on the host.

Experimental Study and Fine-Tuned Simulation of Cable-Joint Pair Slipping in the Cable-Supported Structure

Renzhang Yan,Chunling Yan,Shuai Wang,Chongsheng Cheng,Ting Liu,Hongbo Liu 한국강구조학회 2023 International Journal of Steel Structures Vol.23 No.1

During the tensioning process of the cable-supported structure, the sliding friction between cable wires and joint will have a great influence on the mechanical properties of the structure. However, due to the complex structure of the cable and the combined action of bending deformation and large pre-tension at the cable-strut joint, it is difficult to accurately determine the coefficient of sliding friction (CSF) between cable wires and joint, and thus the overall mechanical properties of the structure cannot be accurately evaluated. Based on the twisting characteristics of the cable, this paper establishes a friction-slip test model and a refined numerical model to carry out detailed study of the mechanical behavior of contact between cable wires and joint during the tensioning process. Firstly, combined with the calculation results of contact mechanics, it is finds that the stress distribution at straight and curved sections of the cable are non-uniform along its axis and each cross section, and the non-uniformity coefficient of the internal force distribution of the cable is further quantified. At the same time, the local bending of the cable will intensify the uneven stress distribution in the circumferential direction of the cable. The non-uniformity of stress reveals the contact working mechanism of the cable-joint pair. Then, the change of the prestress loss between cable wires and joint with the tensioning process is analyzed, and the parametric analysis is conducted to reveal the effect of the cable included angle, tensile force, and lay length on the CSF. The results find that the prestress loss increases approximately linearly with the increase of tension force and decreases with the increase of the cable included angle, but the CSF is less affected by the lay length during the tensioning process. Finally, a method for valuing the CSF of the cable-supported structure is developed.