Acid phosphatase activity in an Acanthamoeba species isolate

Chen, Ko-Hsien,Min, Duk-Young,Kim, Kyung-Min 한양대학교 의과대학 1994 한양의대 학술지 Vol.14 No.2

이 연구에서는 우리나라에서의 담수어에서 분리한 Acanthamoeba species의 생리기능적 구조에 대한 기초적 연구로 세포질내 acid(AcPase)활성능을 관찰하여 다음과 같은 결과를 얻었다. 광학현미경상에서 아메바의 AcPase의 활성을 관찰한 결과 세포내 공포로 여겨지는 부위에서 과립상의 반응을 보였다. 투과전자현미경으로 아메바의 AcPase 활성을 관찰한 결과 대부분의 공포 및 REP에서 AcPase 양성반응을 관찰할 수 있었으며 Golgi-compelx 및 미토콘드리아에서는 AcPase 양성반응을 관찰할 수 없었으나 항혈청으로 처치된 아메바에서는 일부 미토콘드리아에서 Acpase 양성반응을 나타내는 것으로 관찰되었다. 이 때 양성 반응을 보이는 공포들은 (a) 크기가 작으며 구조 전체가 강한 양성 반응을 보이는 lysosome (b) 크기가 크며 한계막 및 내용물이 강한 양성 반응을 보이는 식포들 (c) 전체적으로 한계막과 내용물의 반응이 약한 식포들 (d) 내용물이 있으나 전혀 양성 반응이 없는 식포들 및 (e) 내용물도 없고 음성 반응을 보이는 식포 및 수축포들 등으로 구분할 수 있었다. 또한 강한 Acpase 양성 반응을 보이는 lysosome 또는 vesicle등은 서로 융합하여 커다란 식포를 형성하고 있음이 관찰되었으며 일부, AcPase 양성인 식포는 또 다른 식포 주변에 접해 있거나 융합 또는 연결되어 있음을 관찰하였다. 이상의 결과로 미루어 이 연구에서 관찰된 Acanthamoeaba species는 소화기능에 있어서 lysosome이 서로 융합되거나 다른 식포에 융합, 연결되어 포식된 내용물을 소화하는 것으로 여겨진다.

Molecularly Imprinted Polymers Having Amidine and Imidazole Functional Groups As an Enzyme-Mimetic Catalyst for Ester Hydrolysis

Chen, Wen,Han, Dong-Keun,Ahn, Kwang-Duk The Polymer Society of Korea 2002 Macromolecular Research Vol.10 No.2

A molecularly imprinted polymer (MIP) having both amidine and imidazole functional groups in the active site has been prepared using p-nitrophenyl phosphate as a transition state analogue (TSA). The imprinted polymer MIP with amidine and imidazole found to have the highest hydrolysis activity compared with other MIPs with either amidine or imidazole groups only. It is postulated a cooperative effect between amidine and imidazole in the hydrolysis of p-nitrophenyl methyl carbonate (NPMC) as a substrate when both groups were arranged in proximity by molecular imprinting. The rate enhancement of the hydrolysis by MIP was 60 folds over the uncatalyzed solution reaction and two folds compared with the control non-imprinted polymer CPI having both functional groups. The enzyme-mimetic catalytic hydrolysis of p-nitrophenyl acetate by MIP was evaluated in buffer at pH 7.0 with $K_{m}$ of 1.06 mM and $k_{cat}$ of 0.137 $h^{-1}$ . . .

Molecularly Imprinted Polymers Having Amidine and Imidazole Functional Groups As an Enzyme - Mimetic Catalyst for Ester Hydrolysis

Wen Chen,Dong Keun Han,Kwang Duk Ahn,Jong Man Kim 한국고분자학회 2002 Macromolecular Research Vol.10 No.2

The Mixed Kernel Function SVM-based Point Cloud Classification

Chao Chen,Xiaomin Li,Abdelkader Nasreddine Belkacem,Zhifeng Qiao,Enzeng Dong,Wenjun Tan,Duk Shin 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.20 No.5

Measurement and detection of ground information by airborne Lidar are one of the hot topics in the field of intelligent sensing in recent years. This study proposes a new point cloud classification algorithm of Mixed Kernel Function SVM to distinguish different types of ground objects. Firstly, the combined features including the coordinate values, the RGB value, normalized elevation, standard deviation of elevation, and elevation difference of point cloud data were extracted. A mixed kernel function of Gauss and Polynomial was designed. Then, one-versus-rest SVM multiple classifiers was constructed. Finally, the feature of 3D point cloud data was employed to train the SVM classifiers. The overall classification accuracies of test data were 97.69% and 99.13% for two data sets, I and II respectively. In addition, the experimental results have showed that the performance of the proposed method with mixed kernel function SVM was better than standard SVM method with Gaussian kernel function and polynomial kernel function only, which demonstrates the effectiveness of the proposed method.

Testing of Purchasing Power Parity Theory Using the Doubly Truncated ARMA-GARCH Model and MCMC Algorithms

( Su Duk Kim ),( Chyong Ling Chen ),( Hiroki Tsurumi ) 한양대학교 경제연구소 2003 JOURNAL OF ECONOMIC RESEARCH Vol.8 No.2

Study and Software Implementation of Shield Excavation Guidance System

Jin-duk Lee(이진덕),Ji-xiang Chen(천지시앙) 대한토목학회 2009 대한토목학회 학술대회 Vol.2009 No.10

This article firstly focused on the study of mathematical model about the system, and then given the software design framework of guidance system based on the model, finally we take some example data for quantitative test to ensure the reliability.

중국 전통무술 특징 탐색을 통한 발전전략

이현신 ( Xian Chen Li ),김학덕 ( Hak Duk Kim ),김찬룡 ( Chan Ryong Kim ) 대한무도학회 2011 대한무도학회지 Vol.13 No.2

최근 중국 전통무술은 현대사회의 급진적인 변화와 함께 수련생의 감소와 무술의 침체기를 맞고 있다. 중국 전통무술은 개인의 수련과 품행을 매우 중시하며 무술의 기격성 및 실전가치는 그의 본질적인 특징이라고 할 수 있다. 또한, 전통무술 및 예의의 융합이 전통무술의 중요한 특징이라고 하여 사람들은 전통무술 중 예의를 무덕이라고 칭한다. 이로 인해 전통무술의 특징을 말하자면 기술이나 이론에만 한정할 수 없으며 그에 속하는 일부로써의 무덕까지 고려해야 한다. 현재 중국무술은 전통무술과 경기무술 두 가지 영역이 존재한다. 그러나 이러한 두 가지 영역이 불균형적인 발전추세를 보이고 있다. 이에 본 연구는 중국 전통무술의 문파 특징, 내재적 특징, 경기·예술 특징, 예의적 특징, 전통무술과 경기무술 정의 특징을 탐색하여, 문헌연구를 통하여 중국 전통무술의 역사성과 현대사회의 흐름에 잘 적용할 수 있는 발전전략을 모색하는 것이 본 연구의 목적이다. 이를 통해 도출된 구체적인 결론은 다음과 같다. 첫째, 과학적인 전통무술의 이론을 정립해야 한다. 둘째, 전통무술과 경기무술간의 조화로운 발전을 도모해야 한다. 셋째, 전통무술의 전문기술의 보유 및 수련을 목적으로 하는 것 뿐 만 아니라 건강을 주요목적으로 해야 한다. 넷째, 전통무술의 투로를 보호해야 한다. 다섯째, 대내외의 협조 및 교류를 증가시켜야 한다. 여섯째, 전통 무술을 다양한 행사 및 홍보활동을 통하여 보급을 강화해 나가야 한다. With dramatic changes of the modern society and reduction in trainee numbers, there has been stagnation in recent traditional Chinese martial arts. Chinese traditional martial arts considers the training and demeanor of the individual very importantly and is fundamentally characteristic of its martial nature and actual fighting value. In addition, courtesy is referred to as ``the virtue of martial arts` since it is taught that the fusion of traditional martial art and courtesy is an important characteristic of the traditional martial arts. Because of this, when speaking of the characteristics of traditional martial arts, they are not limited to the techniques or theories but must consider the virtue of martial arts as its integral part. Currently, there are two areas of Chinese traditional martial arts in practice; traditional martial arts and competing martial arts. However, the trend these days is that the two are developing unevenly. Hence, this study investigates the characteristics of various schools of traditional martial arts, their internal features, competition/arts features, courtesy features, and the characteristics of defining traditional art and competing martial arts through literary reviews with an aim to explore development strategies that are applicable to the historicity and the current trends of traditional martial arts. Specific conclusions from the study are as follows. First, scientific theories of traditional Chinese martial arts should be established. Second, harmonious development between traditional Chinese martial arts and its competition is needed. Third, health as well as professional skills retention and training should be placed as the main purpose of traditional martial arts. Fourth, a set pattern of traditional Chinese martial arts should be protected. Fifth, domestic and international cooperation and interaction should increase. Finally, propagation of martial arts should be enhanced by various events and promotions.

The Software Implementation on TBM Supervise System with Expansion of VRS

Lee, Jin-Duk(이진덕),Chen, Ji Xiang(전지장) 한국측량학회 2010 한국측량학회 학술대회자료집 Vol.2010 No.4

Adaptive PI gain Control to Realize Sinusoidal Ripple Current Charging

Jen-Guey Chen,Yong-Duk Lee,Sung-Yeul Park 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6

This paper presents the adaptive PI gain control to regulate sinusoidal ripple current, which is for battery charging applications. Typically, sinusoidal ripple current charging (SRC) is an outstanding charging algorithm due to its high charging efficiency. In order to perform SRC, the frequency sweep function and regulation of high frequency ripple current are required. The typically designed charger would not be able to satisfy the aforementioned requirements because the control bandwidth of a PI controller is designed for constant current profile. Therefore, the fixed PI gain control is limited to regulate the wide range frequency of ripple current. This paper proposes the adaptive PI gain control based on Model Reference Adaptive Control so that it can regulate variable frequency of charging ripple current. The proposed approach is realized for an existing charger without any change of switching devices. As a result, there is no attenuation and phase delay under variable ripple frequency condition. The performance of current control is compared with conventional fixed PI gain and the proposed adaptive variable gain. The proposed method is verified by simulation and experimental results.

동의보감(東醫寶鑑) 중(中) 천화분(天花粉)이 주약(主藥)으로 배오(配伍)된 방제(方劑)의 활용(活用)에 대한 고찰(考察)

서창우,유진덕,Seo Chang-Woo,Liu Chen-Duk 대한한의학방제학회 2003 大韓韓醫學方劑學會誌 Vol.11 No.1

Through the research into the date of Radix Thichosanthis main blended 21 kinds of prescriptions Radix Thichosanthis from Donguibogam. This study summrized as follows; The suitable dosage of Radix Thichosanthis per one application is one to two don(about $4{\sim}8g$). Radix Thichosanthis Radix main blended prescriptions broadly use in the symptoms of diabetes, excessive thirst, cabuncles and eye diseases In diabetes and excessive thirst, the structes of Radix Thichosanthis main blended prescriptions are commonly used Seng Mak San(生脈散), Radix Puerariae(葛根), Frectus Schisandrae(五味子), Radix Ophiopogonis(麥門冬) In Furuncles, Carbuncles and Cancer like diseases, the structes of Radix Thichosanthis main blended prescriptions are generally used Kumeunhwasan(金銀花散), Jipaesan(芷貝散), Twonongsan(透膿散).