Leukocyte invasion of the brain after peripheral trauma in zebrafish (Danio rerio)

Chen Xiang-Ke,Kwan Joseph Shiu-Kwong,Wong Gordon Tin-Chun,Yi Zhen-Ni,Ma Alvin Chun-Hang,Chang Raymond Chuen-Chung 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Despite well-known systemic immune reactions in peripheral trauma, little is known about their roles in posttraumatic neurological disorders, such as anxiety, sickness, and cognitive impairment. Leukocyte invasion of the brain, a common denominator of systemic inflammation, is involved in neurological disorders that occur in peripheral inflammatory diseases, whereas the influences of peripheral leukocytes on the brain after peripheral trauma remain largely unclear. In this study, we found that leukocytes, largely macrophages, transiently invaded the brain of zebrafish larvae after peripheral trauma through vasculature-independent migration, which was a part of the systemic inflammation and was mediated by interleukin-1b (il1b). Notably, myeloid cells in the brain that consist of microglia and invading macrophages were implicated in posttraumatic anxiety-like behaviors, such as hyperactivity (restlessness) and thigmotaxis (avoidance), while a reduction in systemic inflammation or myeloid cells can rescue these behaviors. In addition, invading leukocytes together with microglia were found to be responsible for the clearance of apoptotic cells in the brain; however, they also removed the nonapoptotic cells, which suggested that phagocytes have dual roles in the brain after peripheral trauma. More importantly, a category of conserved proteins between zebrafish and humans or rodents that has been featured in systemic inflammation and neurological disorders was determined in the zebrafish brain after peripheral trauma, which supported that zebrafish is a translational model of posttraumatic neurological disorders. These findings depicted leukocyte invasion of the brain during systemic inflammation after peripheral trauma and its influences on the brain through il1b-dependent mechanisms.

Depth Distributions of Bi+ Ions Implanted into Ni, Si and SiO₂, Films

Ke-Ming Wang,Feng Chen,Xue-Lin Wang,Jian-Hua Zhang,Xiang-Dong Liu 한국진공학회(ASCT) 2002 Journal of Korean Vacuum Science & Technology Vol.6 No.1

Ni, Si and SiO₂ films were implanted by 350 keV Bi^+ ions at room temperature with fluences of 1×10^(16) and 2×10^(16) ions/㎤. The depth distributions of implanted Bi^+ ions in Ni, Si and SiO₂ films were by investigated by Rutherford backscattering. The results show that the depth distributions of implanted Bi^+ ions into Ni, Si and SiO₂ films have obeyed nearly Gaussian distributions. The maximum difference between experimental and calculated values is less than 18 % for mean projected range. Experimental range straggling deviated significantly from calculated value. The possible reasons are discussed.

Tools Setting of 5 Axis Ultra-Precision Single point Diamond Lathe without OTS

Ke-Tian Li,Xin Chen,Xin-Du Chen,Qiang Liu,Xiang-bo Ouyang,Hong-Jian Xia,Jian-Qun Liu 한국생산제조학회 2014 한국생산제조시스템학회 학술발표대회 논문집 Vol.2014 No.9(2)

X-Ray Repair Cross-Complementing Group 1(XRCC1) Genetic Polymorphisms and Thyroid Carcinoma Risk: a Meta-Analysis

Qian, Ke,Liu, Kui-Jie,Xu, Feng,Chen, Xian-Yu,Chen, Gan-Nong,Yi, Wen-Jun,Zhou, En-Xiang,Tang, Zhong-Hua Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

A number of studies have been conducted to explore the association of XRCC1 polymorphisms with thyroid cancer risk, but the results have been inconsistent. Thus we performed the present meta-analysis to clarify this issue based on all of the evidence available to date. Relevant studies were retrieved by searching PubMed and statistical analysis conducted using Stata software. Nine studies were included in this meta-analysis (1,620 cases and 3,557 controls). There were 6 studies (932 cases and 2,270 controls) of the Arg194Trp polymorphism, 7 studies (1432 cases and 3356 controls) of the Arg280His polymorphism and 9 studies (1,620 cases and 3,557 controls) for the Arg399Gln polymorphism. No association of XRCC1 Arg194Trp, Arg280His and Arg399Gln polymorphism with thyroid cancer risk was observed in the overall analysis. However, subgroup analysis revealed: 1) an elevated risk in aa vs AA analysis (OR=2.03, 95%CI= 1.24-3.31) and recessive genetic model analysis (OR=1.93, 95%CI= 1.20-3.08) in the larger sample size trials for XRCC1 Arg194Trp polymorphism; 2) a decreased thyroid cancer risk on subgroup analysis based on ethnicity in Aa vs AA analysis (OR=0.84, 95%CI= 0.72-0.98) and in a dominant genetic model (OR=0.84, 95%CI= 0.72-0.97) in Caucasian populations for the XRCC1 Arg399Gln polymorphism; 3) a decreased thyroid cancer risk on subgroup analysis based on design type in Aa vs AA analysis (OR=0.72, 95% CI= 0.54-0.97) among the PCC trials for the Arg399Gln polymorphism. Our results suggest that the XRCC1 Arg399Gln polymorphism may be associated with decreased thyroid cancer risk among Caucasians and XRCC1 Arg194Trp may be associated with a tendency for increased thyroid cancer risk in the two larger sample size trials.

Study on the Control and Projective Synchronization of a New Fractional-order Chaos System

Shao Ke-Yong,Chen Bai-Quan,Wang Ting-Ting,Xu Xiang,Zhang Ting-Ting,Ma Di 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.10

In this paper, a new fractional-order chaotic system was studied, and the basic dynamic properties of the new system were analyzed. The control of the new system in unstable equilibrium point was realized by designing the reasonable nonlinear controller. Further, we investigated the projective synchronization problem about fractional order chaotic systems with different dimensions, the appropriate controller was designed to achieve the projective synchronization of a four-dimensional drive system and a three-dimensional response system by reducing the dimension. With the numerical simulation of Matlab, the feasibility of the scheme was verified.

Investigation of Planar Optical Waveguide Formed by MeV He+ Ion - Implantation into NaEr(WO₄)₂ Crystal

Feng Chen,Xue-Lin Wang,Ke-Ming Wang,Zhen-Xiang Cheng,Huan-Chu Chen,Ding-Yu Shen 한국진공학회(ASCT) 2002 Journal of Korean Vacuum Science & Technology Vol.6 No.2

NaEr(WO₄)₂ is a new laser material. The planar optical waveguide was formed in NaEr(WO₄)₂ crystal by 2.6 MeV He^+ ion implantation at doses of 1.0-1.5×10^(16) ions/㎤ at room temperature. The effective refractive indices of the dark modes were measured using the prism coupling method. Four TE modes and five TM modes were observed in the waveguide. The refractive index profiles were analyzed using the reflectivity calculation method (RCM). The influence of heat treatment at moderate temperature on the refractive index profiles of the waveguide was also investigated. We used the TRIM’98 (Transport of Ions in Matter) code to simulate the damage profile in the NaEr(WO₄)₂ crystal by 2.6 MeV He^+ ion implantation which is helpful for a better understanding of the waveguide formation.

Targeted Efficacy of Dihydroartemisinin for Translationally Controlled Protein Expression in a Lung Cancer Model

Liu, Lian-Ke,Wu, Heng-Fang,Guo, Zhi-Rui,Chen, Xiang-Jian,Yang, Di,Shu, Yong-Qian,Zhang, Ji-Nan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Objective: Lung cancer is one of the malignant tumors with greatest morbidity and mortality around the world. The keys to targeted therapy are discovery of lung cancer biomarkers to facilitate improvement of survival and quality of life for the patients with lung cancer. Translationally controlled tumor protein (TCTP) is one of the most overexpressed proteins in human lung cancer cells by comparison to the normal cells, suggesting that it might be a good biomarker for lung cancer. Materials and Methods: In the present study, the targeted efficacy of dihydroartemisinin (DHA) on TCTP expression in the A549 lung cancer cell model was explored. Results and Conclusions: DHA could inhibit A549 lung cancer cell proliferation, and simultaneously up-regulate the expression of TCTP mRNA, but down-regulate its protein expression in A549 cells. In addition, it promoted TCTP protein secretion. Therefore, TCTP might be used as a potential biomarker and therapeutic target for non-small cell lung cancers.

Finite Element Analysis and Test Study on Restraint of High-energy Pipe Whip in Conventional Island

Ling-yun Peng,Yu-ke Deng,Hua-ting Chen,Ying-jie Kang,Xiang-xiu Li 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.4

The effects of high-energy pipe whipping after rupturing are very important in an AP1000 nuclear power plant's conventional island and restraints of pipe whipping should be considered in the design. It is the first time in the country when reinforced concrete shear walls are used as restraint services. In this paper, the behaviors of walls and restraint services subjected to pipe whipping are analyzed through static and dynamic methods in the finite element software (ABAQUS), in which all kinds of nonlinearities are considered. In addition, a test study on the restraint of pipe whipping is conducted. The results show that the wall and the restraint service can prevent pipe whipping effectively under the design load, and anchor plates arranged around the wall opening can improve the local concrete compression performance of concrete to reduce the damage of concrete. Meanwhile, the study also provides the valuable reference for wall designing to prevent from pipe whipping.

Population dynamics, hunting nature on insect pests and existence of symbiotic bacterial microbes among leading transgenic cotton spiders

Nasir Muhammad,Zhao Chen-chen,Luo Jun-yu,Zhu Xiang-zhen,Gao Xue-ke,Iqbal Mazher F.,Ji Ji-chao,Zhang Shuai,Cui Jin-jie 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1

The genetically modified cotton holding Bt proteins, is noxious to bollworms larvae but very little is known about its impact on population of non-target spiders within the field. Studies conducted with the aim to identify spiders along with their abundance, prevalence pattern, preying habit on insect pests in Bt cotton (L280, Cry2Ab4) and commonness of symbiotic bacteria in these spiders. Spiders collections were made via vial tapping/jarring, from May to end of October (2018). In total, 13,342 spiders were recorded during the entire cropping season. Least relative abundance was in May (0.28%), highest in August (30.39%) while ahead decline was observed, reached to 12% in October. The 27 species were verified, least richness was in May (3 species), high in August (19 species), later in the end, reduced to 7 species. High abundance was of Hylyphantes graminicola (68.56%) and Neoscona theisi (19.98%). Existence of H. graminicola and Pardosa astrigera observed during the whole cropping season. Presence of seven guilds among spiders had a diverse nature of hunting insect pests and on number of species existed in the crop, maximum was from direct hunting habit. Composition and community structure of microbiota varied as of spider species. Proteobacteria was the most abundant bacterial phylum and topmost genus was Wolbachia in observed spiders. Five groups of bacteria distinguished across 4 clusters of spiders depending on core community of symbiotic bacterial genera. This effort is an initial step to get knowhow about spiders in Bt cotton, which will facilitate future research studies concerning spiders.

Marein Prevented LPS-Induced Osteoclastogenesis by Regulating the NF-κB Pathway In Vitro

( Yuling Li ),( Jing Zhang ),( Caiping Yan ),( Qian Chen ),( Chao Xiang ),( Qingyan Zhang ),( Xingkuan Wang ),( Ke Jiang ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.2

Many bone diseases such as osteolysis, osteomyelitis, and septic arthritis are caused by gramnegative bacterial infection, and lipopolysaccharide (LPS), a bacterial product, plays an essential role in this process. Drugs that inhibit LPS-induced osteoclastogenesis are urgently needed to prevent bone destruction in infective bone diseases. Marein, a major bioactive compound of Coreopsis tinctoria, possesses anti-oxidative, anti-inflammatory, anti-hypertensive, antihyperlipidemic, and anti-diabetic effects. In this study, we measured the effect of marein on RAW264.7 cells by CCK-8 assay and used TRAP staining to determine osteoclastogenesis. The levels of osteoclast-related genes and NF-κB-related proteins were then analyzed by western blot, and the levels of pro-inflammatory cytokines were quantified by ELISA. Our results showed that marein inhibited LPS-induced osteoclast formation by osteoclast precursor RAW264.7 cells. The effect of marein was related to its inhibitory function on expressions of pro-inflammatory cytokines and osteoclast-related genes containing RANK, TRAF6, MMP-9, CK, and CAII. Additionally, marein leads to markedly inhibited NF-κB signaling pathway activation in LPS-induced RAW264.7 cells. Concurrently, when the NF-κB signaling pathway was inhibited, osteoclast formation and proinflammatory cytokine expression were decreased. Collectively, marein could inhibit LPS-induced osteoclast formation in RAW264.7 cells via regulating the NF-κB signaling pathway. Our data demonstrate that marein might be a potential drug for bacteria-induced bone destruction disease. Our findings provide new insights into LPS-induced bone disease.