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Chelliah, Ramachandran,Saravanakumar, Kandasamy,Daliri, Eric Banan-Mwine,Kim, Joong-Hark,Lee, Jung-Kun,Jo, Hyeon-yeong,Kim, Se-Hun,Ramakrishnan, Sudha Rani,Madar, Inamul Hasan,Wei, Shuai,Rubab, Momna Elsevier 2020 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.143 No.-
<P><B>Abstract</B></P> <P>Human-milk-based probiotics play a major role in the early colonization and protection of infants against gastrointestinal infection. We investigated potential probiotics in human milk. Among 41 Lactic acid bacteria (LAB) strains, four strains showed high antimicrobial activity against <I>Escherichia coli</I> 0157:H7, <I>Listeria monocytogenes</I> ATCC 15313, <I>Bacillus cereus</I> ATCC 14576, <I>Staphylococcus aureus</I> ATCC 19095, and <I>Helicobacter pylori</I>. The selected LAB strains were tested in simulated gastrointestinal conditions for their survival. Four LAB strains showed high resistance to pepsin (82%–99%), bile with pancreatine stability (96%–100%), and low pH (80%–94%). They showed moderate cell surface hydrophobicity (22%–46%), auto-aggregation abilities (12%–34%), and 70%–80% co-aggregation abilities against <I>L. monocytogenes</I> ATCC 15313, <I>S. aureus</I> ATCC 19095, <I>B. cereus</I> ATCC 14576, and <I>E. coli</I> 0157:H7. All four selected isolates were resistant to gentamicin, imipenem, novobiocin, tetracycline, clindamycin, meropenem, ampicillin, and penicillin. The results show that <I>Pediococcus acidilatici</I> is likely an efficient probiotic strain to produce < 3 Kda pediocin-based antimicrobial peptides, confirmed by applying amino acid sequences), using liquid chromatography mass spectrometry and HPLC with the corresponding sequences from class 2 bacteriocin, and based on the molecular docking, the mode of action of pediocin was determined on LipoX complex, further the <SUP>13</SUP>C nuclear magnetic resonance structural analysis, which confirmed the antimicrobial peptide as pediocin.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Isolation, biochemical and molecular characterization of human milk isolates. </LI> <LI> Probiotic Characterization for the Isolated lactic acid bacteria. </LI> <LI> In vitro antimicrobial activity – Disc diffusion and Growth curve MIC method. </LI> <LI> Antimicrobial peptide characterization and molecular interaction Antimicrobial mechanism – Post biotic efficacy towards pathogens. </LI> <LI> Safety on Probiotic based on ex vivo analysis, in-vivo <I>C. elegans</I> and mice model. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
CHELLIAH RAMACHANDRAN,오덕환 한국식품위생안전성학회 2020 한국식품위생안전성학회지 Vol.35 No.4
Galacto-oligosaccharides (GOS) are prebiotics that have a beneficial effect on human health by promoting the growth of probiotic bacteria in the gut, in addition to having various applications in the food industry. GOS are generally produced from lactose in a reaction catalyzed by ß-galactosidase. Synthesis of GOS from whey permeate (WP) (ultrafiltration of whey, concentrated then spray dried) using surface engineered β-galactosidase in Pichia pastoris (P. pastoris) is a novel method to convert waste into a valuable product. Cell-surface display is the expression of peptides and proteins on the surface of living cells by fusing them to functional components of cells. Surface engineered cells have many potential uses. The Flo1p flocculation functional domain, thought to be located near the N terminus, recognizes and adheres non-covalently to cell-wall components such as α-mannan carbohydrates, causing reversible aggregation of cells into flocs.
Chelliah, Ramachandran,Wei, Shuai,Park, Byung-Jae,Park, Joong-Hyun,Park, Youn-Seo,Kim, Se-Hun,Jin, Yong-Guo,Oh, Deog-Hwan Elsevier 2019 Microbial Pathogenesis Vol.126 No.-
<P><B>Abstract</B></P> <P> <I>Bacillus thuringiensis</I> promotes the growth of numerous economically important crops. The present study presents the complete genome sequence for a mega plasmid present in the type strain of <I>B. thuringiensis</I> ATCC 10792, a typical spore-forming Gram-positive bacterium with insecticidal activity, and investigates its genetic characteristics. The genome was sequenced and assembled de novo using Pac-Bio sequencers and the Hierarchical Genome Assembly Process, respectively. Further genome annotation was performed, and a total of 489 proteins and a novel mega-plasmid (poh1) with 584,623 bps were identified. The organization of poh1 revealed the genes involved in the insecticidal toxin pathway. The genes responsible for antimicrobial, insecticidal and antibiotic activities were well conserved in poh1, indicating an intimate association with plant hosts. The poh1 plasmid contains the gene encoding a novel crystal protein kinase responsible for production of zeta toxin, which poisons insects and other Gram-negative bacteria through the global inhibition of peptidoglycan synthesis. Lantibiotics are a group of bacteriocins that include the biologically active antimicrobial peptide <I>Paenibacillin</I>. Further, poh1 also contains the genes that encode the gramicidin S prototypical antibiotic peptide and tetracycline resistance protein. In conclusion, the strain-specific genes of <I>B. thuringiensis</I> strain ATCC 10792 were identified through complete genome sequencing and bioinformatics data based on major pathogenic factors that contribute to further studies of the pathogenic mechanism and phenotype analyses.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Complete genome sequence of Mega plasmid (poh1) in <I>B. thuringiensis</I> ATCC 10792. </LI> <LI> The plasmid encodes the Paenibacillin proteins (nisin, bisin, subtilin, epidermin and gallidermin) for lantibiotic family. </LI> <LI> The plasmid harbors a novel crystal like protein and zeta toxin, Tetracycline resistance gene in the mega plasmid. </LI> <LI> Specific gene with selective proteins, and its information has been deposited in Uni-Prot (https://www.uniprot.org/uniprot/AA0A0BU976). </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Role of Peptides in Antiviral (COVID-19) Therapy
Chelliah, Ramachandran,Daliri, Eric Banan-Mwine,Elahi, Fazle,Yeon, Su-Jung,Tyagi, Akanksha,Park, Chae Rin,Kim, Eun Ji,Jo, kyoung Hee,Oh, Deog-Hwan The Korean Society of Food Hygiene and Safety 2021 한국식품위생안전성학회지 Vol.36 No.5
COVID-19와 같은 전염병 감염 시나리오 전반에 걸쳐 펩타이드 기반 치료법을 발견하고 설계하는 개발 시대의 추세는 보다 효율적이고 저렴한 치료 환경으로 발전할 수 있습니다. 결과적으로, 그들의 단백질 분해 약화는 천연펩타이드 약물의 단점 중 하나입니다. 펩티도미메틱스는 이 단점을 해결하는 데 도움이 될 수 있습니다. 이 리뷰에서 펩타이드 및 펩타이드 기반 약물 발견은 숙주 안지오텐신 전환 효소-2(ACE2) 수용체 및 바이러스 스파이크 (S)단백질의 연관성을 포함하는 중증 코로나바이러스 폐색전 증후군(SARS-CoV-2)의 주요 진입 기전 중 하나를 표적으로 요약했습니다. 또한, 펩타이드 기반의 새로운 치료법을 통해 COVID-19에 대해 연구된 단백질, 펩타이드 및 기타 가능한 조치의 이점을 다룹니다. 그리고 펩타이드 기반 약물 치료 환경의 개요는 진화적 관점, 구조적 특성, 작동 한계값 및 치료 영역에 대한 설명으로 구성된다 Trends in the developing era to discover and design peptide-based treatments throughout an epidemic infection scenario such as COVID-19 could progress into a more efficient and low-cost therapeutic environment. However, the weakening of proteolysis is one downside of natural peptide drugs. But, peptidomimetics may help resolve this issue. In this review, peptide and peptide-based drug discovery were summarized to target one key entry mechanism of severe coronavirus pulmonary emboli syndrome (SARS-CoV-2), which encompasses the association of the host angiotensin-converting enzyme-2 (ACE2) receptor and viral spike (S) protein. Furthermore, the benefits of proteins, peptides and other possible actions that have been studied for COVID-19 through new peptide-based treatments are discussed in the review. Lastly, an overview of the peptide-based drug therapy environment is comprised of an evolutionary viewpoint, structural properties, operational thresholds, and an explanation of the therapeutic area.
( Chelliah R. ),( S. R. Ramakrishnan ),( U. Antony ),( S. H. Kim ),( I. Khan ),( C. N. Tango ),( P. N. Kounkeu ),( S. Wei ),( M. S. Hussain ),( E. B. M. Daliri ),( R. Momna ),( M. Y. Kwon ),( E. H. Le 강원대학교 농업생명과학연구원(구 농업과학연구소) 2018 강원 농업생명환경연구 Vol.30 No.1
Plant proteins are important functional ingredients in many processed food products. In particular, globular proteins from various sources play an important role in many food products. In the current study, 11S globulin protein from white sesame seeds, amandin protein from almond seeds, and cucurbitin protein from pumpkin seeds were extracted by means of ammonium sulfate precipitation and purified by anion-exchange chromatography on a DEAE-Sephadex column (20 × 30 cm). Amandin protein of almond and cucurbitin protein of pumpkin seeds both belong to the 11S globulin family. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of protein samples treated with reducing agents revealed that the isolated 11S globulin from white sesame seeds was composed of an acidic (30-33 kDa) and basic (20-24 kDa) polypeptide, amandin from almond seeds was composed of an acidic (40-42 kDa) and basic (15-20 kDa) polypeptide, and cucurbitin from pumpkin seeds was composed of an acidic (35-42 kDa) and basic (20-25 kDa) polypeptide. The alpha amylase-inhibiting activities of the three proteins was determined. Cucurbitin of pumpkin seeds had a greater alpha amylase inhibitory effect (-86%) than 11S globulin of sesame (82.6%) and amandin protein of almond seeds (76%). The antihypertensive effect of the three proteins was evaluated by a chrioallantoin membrane assay in chick embryos, which revealed that cucurbitin protein showed higher vasodilatation activity than the other two proteins.
Role of Antihypertensive Peptides from Plant (sesame, almond and pumpkin) Seed Storage Protein
( Chelliah R. ),( S. R. Ramakrishnan ),( D. H. Oh ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2017 강원 농업생명환경연구 Vol.29 No.3
Proteins are a potential source of health-promoting biomolecules with medical, nutraceutical, and/or food applications. In particular, plant proteins, including globular proteins, represent important functional ingredients in many processed food products. Thus, the current study investigated and compared the antihypertensive properties of globulin, amandin, and cucurbitin proteins isolated from white sesame, almond, and pumpkin seeds, respectively. Extracted globulin, amandin, and cucurbitin proteins were shown to each comprise acidic and basic polypeptides of varying sizes (globulin polypeptides, 30-33 and 20-24 kDa, respectively; amandin polypeptides, 40-42 and 15-20 kDa, respectively; cucurbitin polypeptides, 35-42 and 20-25 kDa, respectively). The alpha amylase-inhibiting activity and resulting antihypertensive effects exhibited by the isolated 11S globulin, amandin, and cucurbitin polypeptides were characterized. Finally, the current study also reviewed current literature with regard to the properties of various peptides released by plant proteases, and their mitigating effects on disorders such as hypertension, diabetes, obesity, and cancer.
Chelliah, Ramachandran,Wei, Shuai,Park, Byung-Jae,Kim, Se-Hun,Park, Dong-Suk,Kim, Soon Han,Hwan, Kim Seok,Oh, Deog-Hwan Academic Press 2017 Microbial pathogenesis Vol.111 No.-
<P><B>Abstract</B></P> <P>Quantitative triplex real-time PCR (qPCR) offers an alternative method for detection of bacterial contamination. It provides quantitation of the number of gene copies. In our study, we established a qPCR assay to detect and quantify the specificity towards <I>Bacillus cereus</I> and <I>B. thuringiensis</I>. The assay was designed to detect a 280 bp fragment of motB gene encoding the flagellar motor protein, specific for detection of <I>B. cereus</I> and <I>B. thuringiensis</I>, excluding other group species <I>B. pseudomycoides, B. mycoides</I> and <I>B. weihenstephanensis</I>. Specificity of the assay was confirmed with 111 strains belonging to <I>Bacillus cereus</I> group and performed against 58 <I>B. cereus</I>, 50 <I>B. thuringiensis</I>, 3 other <I>Bacillus</I> bacteria and 9 non-<I>Bacillus</I> bacteria. Detection limit was determined for each assay. Direct analysis of samples revealed the specificity towards identification and characterization of <I>B. cereus</I> group cultured in nutrient media. Based on results, it was observed that motB showed 97% specificity towards <I>B. cereus</I> strains, 98% for <I>B. thuringiensis</I> but other <I>B. cereus</I> group showed less sensitivity (0%), thus, provides an efficient tool to identify <I>B. cereus</I> and <I>B. thuringiensis</I>. Further, environmental and food samples do not require band isolation, re-amplification or sequence identification. Thus, reducing the time and cost of analysis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Flagellar motor protein primer OPL-105-motB showed 95% specificity towards <I>B. cereus</I> group. </LI> <LI> Sensitivity of the newly developed primers was confirmed with 120 bacterial strains. </LI> <LI> Biomarkers were developed to detect <I>B. cereus</I> contamination in food industries. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Factor Algebras of Signed Brauer's Algebras
Selvaraj, Chelliah Department of Mathematics 2007 Kyungpook mathematical journal Vol.47 No.4
In this paper we obtain a decomposition of certain factors of the signed Brauer algebra into a direct sum of simple algebras and we obtain the structure of the factor algebra.