Tracklet-Global Track Fusion Using Support Degree Function in Sensor Networks

Xiaobin Li,En Fan,Changhong Yuan,Pengfei Li 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.10

For the situation with unknown qualities of local tracks in sensor networks, a new tracklet-global track fusion method using the support degree function (SDF-T2GTF) is proposed. According to the characteristic of actual transmission modes, two local estimates of a moving target in adjacent interval transmitted by the same local node are defined as a tracklet, and subsequently tracklet-global track (T2GT) fusion can replace the traditional track fusion in the global node, namely local track-global track (LT2GT) fusion. Considered the advantage of the fuzzy track association (TA) method for unknown prior information of local tracks, it is used in T2GT association. Then all correlated tracklets in the same interval can be mapped into a set of points in parameter space by the Hough transform (HT) algorithm. The support degree function of these points is utilized to dynamically estimate the qualities of tracklets and reasonably allocates the weights of local estimates in fusion results. Hence, the proposed method can realize T2GT fusion without the prior information of local tracks. The experimental result illustrates that the proposed method can satisfy the requirement of data transmission in real systems, and can realize T2GT fusion; on the other, it can improve the performance of track fusion in accuracy compared with the traditional methods.

Upregulation of long non-coding RNA XIST has anticancer effects on epithelial ovarian cancer cells through inverse downregulation of hsa-miR-214-3p

Changhong Wang,Shan Qi,Cheng Xie,Chunfu Li,Pu Wang,Dongmei Liu 대한부인종양학회 2018 Journal of Gynecologic Oncology Vol.29 No.6

Objective: The present study is to evaluate the biological functions of long non-coding RNA (lncRNA), X-inactive specific transcript, X-inactive specific transcript (XIST) in human epithelial ovarian cancer (EOC). Methods: XIST was upregulated in EOC cell lines, CAOV3 and OVCAR3 cells by lentiviral transduction. The effects of XIST overexpression on cancer cell proliferation, invasion, chemosensitivity and in vivo tumor growth were investigated, respectively. Possible sponging interaction between XIST and human microRNA hsa-miR-214-3p was further evaluated. Furthermore, hsa-miR-214-3p was overexpressed in XIST-upregulated CAOV3 and OVCAR3 cells to evaluate its effect on XIST-mediated EOC regulation. Results: Lentivirus-mediated XIST upregulation had significant anticancer effects in CAOV3 and OVCAR3 cells by suppressing cancer cell proliferation, invasion, increasing cisplatin chemosensitivity and inhibiting in vivo tumor growth. Hsa-miR-214-3p was confirmed to directly bind XIST, and inversely downregulated in XIST-upregulated EOC cells. In EOC cells with XIST upregulation, secondary lentiviral transduction successfully upregulated hsa-miR-214-3p expression. Subsequently, hsa-miR-214-3p upregulation functionally reversed the anticancer effects of XIST-upregulation in EOC. Conclusion: Upregulation of lncRNA XIST may suppress EOC development, possibly through sponging effect to induce hsa-miR-214-3p downregulation.

A Sphere Filling Algorithm for Irregular Aggregate Particle Generation based on Nonlinear Optimization Method

Changhong Zhou,Hongzhi Yue,Yuhua Li,Miaomiao Zhang,Jiayin Liu,Shahroz Aijaz 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.1

The angularity of particles has important effects on the mechanical properties of asphalt mixture and other granular materials. To simulate these effects, the glue-sphere method was usually used to create an arbitrary polyhedron particle. Unlike other studies, this paper aims to efficiently fill a polyhedron with as few spheres as possible through optimization technology in order to reduce the cost of calculation during mixture simulation. Four contents are mainly discussed here: a) how to produce non-spherical aggregates and control their sizes with the minimal bounding box; b) how to fill convex non-spherical particles with the fewest balls as possible using the constrained nonlinear optimization method; c) how to compute the typical shape factors of these particles; and d) how the control parameters affect the filling effect. The algorithm for this study was programmed by MATLAB software and was proven to have better filling performance and less computational cost compared to other methods.

Single-cell transcriptomic analysis reveals transcriptional and cell subpopulation differences between human and pig immune cells

Li Jie,Xu Yanan,Zhang Jiayu,Zhang Zhaoqi,Guo Han,Wei Dong,Wu Changhong,Hai Tang,Sun Hai-Xi,Zhao Yong 한국유전학회 2024 Genes & Genomics Vol.46 No.3

Background The pig is a promising donor candidate for xenotransplantation. Understanding the differences between human and swine immune systems is critical for addressing xenotransplant rejection and hematopoietic reconstitution. The gene transcriptional profile differences between human and pig immune cell subpopulations have not been studied. To assess the similarities and differences between pigs and humans at the levels of gene transcriptional profiles or cell subpopulations are important for better understanding the cross-species similarity of humans and pigs, and it would help establish the fundamental principles necessary to genetically engineer donor pigs and improve xenotransplantation. Objective To assess the gene transcriptional similarities and differences between pigs and humans. Methods Two pigs and two healthy humans’ PBMCs were sorted for 10 × genomics single-cell sequence. We generated integrated human-pig scRNA-seq data from human and pig PBMCs and defined the overall gene expression landscape of pig peripheral blood immune cell subpopulations by updating the set of human-porcine homologous genes. The subsets of immune cells were detected by flow cytometry. Results There were significantly less T cells, NK cells and monocytes but more B cells in pig peripheral blood than those in human peripheral blood. High oxidative phosphorylation, HIF-1, glycolysis, and lysosome-related gene expressions in pig CD14+ monocytes were observed, whereas pig CD14+ monocytes exhibited lower levels of cytokine receptors and JAK-STAT-related genes. Pig activated CD4+T cells decreased cell adhesion and inflammation, while enriched for migration and activation processes. Porcine GNLY+CD8+T cells reduced cytotoxicity and increased proliferation compared with human GNLY+CD8+T cells. Pig CD2+CD8+γδT cells were functionally homologous to human CD2+CD4+ γδT cells. Pig CD2−CD8−γδT cells expressed genes with quiescent and precursor characteristics, while CD2−CD8+γδT cells expressed migration and memory-related molecules. Pig CD24+ and CD5+B cells are associated with inflammatory responses. Conclusion Our research with integrated scRNA-seq assays identified the different distribution of pig immune cell subpopulations and the different transcriptional profiles of human and pig immune cells. This study enables a deeper understanding of the development and function of porcine immune cells. Background The pig is a promising donor candidate for xenotransplantation. Understanding the differences between human and swine immune systems is critical for addressing xenotransplant rejection and hematopoietic reconstitution. The gene transcriptional profile differences between human and pig immune cell subpopulations have not been studied. To assess the similarities and differences between pigs and humans at the levels of gene transcriptional profiles or cell subpopulations are important for better understanding the cross-species similarity of humans and pigs, and it would help establish the fundamental principles necessary to genetically engineer donor pigs and improve xenotransplantation. Objective To assess the gene transcriptional similarities and differences between pigs and humans. Methods Two pigs and two healthy humans’ PBMCs were sorted for 10 × genomics single-cell sequence. We generated integrated human-pig scRNA-seq data from human and pig PBMCs and defined the overall gene expression landscape of pig peripheral blood immune cell subpopulations by updating the set of human-porcine homologous genes. The subsets of immune cells were detected by flow cytometry. Results There were significantly less T cells, NK cells and monocytes but more B cells in pig peripheral blood than those in human peripheral blood. High oxidative phosphorylation, HIF-1, glycolysis, and lysosome-related gene expressions in pig CD14+ monocytes were observed, whereas pig CD14+ monocytes exhibited lower levels of cytokine receptors and JAK-STAT-related genes. Pig activated CD4+T cells decreased cell adhesion and inflammation, while enriched for migration and activation processes. Porcine GNLY+CD8+T cells reduced cytotoxicity and increased proliferation compared with human GNLY+CD8+T cells. Pig CD2+CD8+γδT cells were functionally homologous to human CD2+CD4+ γδT cells. Pig CD2−CD8−γδT cells expressed genes with quiescent and precursor characteristics, while CD2−CD8+γδT cells expressed migration and memory-related molecules. Pig CD24+ and CD5+B cells are associated with inflammatory responses. Conclusion Our research with integrated scRNA-seq assays identified the different distribution of pig immune cell subpopulations and the different transcriptional profiles of human and pig immune cells. This study enables a deeper understanding of the development and function of porcine immune cells.

Study on Mud-Water Separation Test Technology for Shield Slurry in Underground Tunnel

( Changhong Yan ),( Shulan Guo ),( Liang Wen ),( Liangwei Li ),( Xiaoyong Yang ),( Zhiwei Jin ),( Xuyang Fan ) 대한지질공학회 2019 대한지질공학회 학술발표회논문집 Vol.2019 No.2

The construction of urban subway produced a large amount of slurry with hign moisture content. In order to ensure the stability of the excavation face during the construction of the tunnel shield, prevent the spoil from adhering to the shield cutter, a large amount of additives such as water, bentonite, foaming agent are added to the spoil. Therefore, the slurry has the characteristics of uneven particle composition, high moisture content, low permeability and remarkable plastic flow, and its shear strength is almost zero. The transportation and stacking of slurry can cause environmental pollution and damage the ecological environment. To solve this problem, our research team developed a set of mud-water separation test device to carry out research on mud-water separation technology. The Optimal treatment plan for mud-water separation is given by a large number of laboratory tests: when the moisture content of slurry is relatively high, the vacuum negative pressur is firstly used to discharge the free water. When the moisture content drops below the liquid limit, the electroosmosis and the vacuum negative pressure are combined to discharge the bound water, at which time the moisture content in the slurry is close to the plastic limit, thereby achieving slurry hardening. This method has a fast drainage speed and low cost, and has been successfully applied to the Suzhou Metro. It is not only conducive to the resource utilization of slurry, but more importantly, it can effectively protect the ecological environment.

Review : Construction of a Genetic System for Streptomyces albulus PD-1 and Improving Poly(ε-L-lysine) Production Through Expression of Vitreoscilla Hemoglobin

( Zhaoxian Xu ),( Changhong Cao ),( Zhuzhen Sun ),( Sha Li ),( Zheng Xu ),( Xiaohai Feng ),( Hong Xu ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.11

Poly(ε-L-lysine) (ε-PL) is a novel bioactive polymer secreted by filamentous bacteria. Owing to lack of a genetic system for most ε-PL-producing strains, very little research on enhancing ε-PL biosynthesis by genetic manipulation has been reported. In this study, an effective genetic system was established via intergeneric conjugal transfer for Streptomyces albulus PD-1, a famous ε-PL-producing strain. Using the established genetic system, the Vitreoscilla hemoglobin (VHb) gene was integrated into the chromosome of S. albulus PD-1 to alleviate oxygen limitation and to enhance the biosynthesis of ε-PL in submerged fermentation. Ultimately, the production of ε-PL increased from 22.7 g/l to 34.2 g/l after fed-batch culture in a 5 L bioreactor. Determination of the oxygen uptake rate, transcriptional level of ε-PL synthetase gene, and ATP level unveiled that the expression of VHb in S. albulus PD-1 enhanced ε-PL biosynthesis by improving respiration and ATP supply. To the best of our knowledge, this is the first report on enhancing ε-PL production by chromosomal integration of the VHb gene in an ε-PL-producing strain, and it will open a new avenue for ε-PL production.

Investigation of Some Hard Coatings Synthesized by Ion Beam Assisted Deposition

Jianli He,Wen-Zhi Li,Xiaoming He,Changhong Liu 한국진공학회 1995 한국진공학회 학술발표회초록집 Vol.- No.-

Theoretical investigation of deformation characteristics of stratified rocks considering geometric and mechanical variability

Peitao Wang,Meifeng Cai,Fenhua Ren,Changhong Li,Tianhong Yang 한국지질과학협의회 2017 Geosciences Journal Vol.21 No.2

Anisotropy is one of the most distinct features of stratified rock mass and it must be considered in engineering design and stability analysis. On the basis of linear elastic theory and displacements equivalence, a computational model considering elastic anisotropy was established. The equivalent elastic modulus and equivalent Poisson’s ratio of the stratified rock perpendicular and parallel to the loading direction were discussed. The relations of material properties and geometry parameters with the equivalent elastic modulus and equivalent Poisson’s ratio in two directions were studied. Uniaxial compressive test was conducted and the elastic modulus and Poisson’s ratio in the proposed model agreed well with experimental results. The comparison between a comprehensive set of experimental data and theoretical analysis data proves that the proposed constitutive model can effectively characteristic the mechanical behavior of stratified rocks. The proposed model can consider the effects of material parameters and geometry parameters of rock and joints on the mechanical behavior of stratified rock mass. Moreover, the elastic anisotropy was considered in the model. Thus, the model can supply some valuable reference in studying deformation of stratified rock mass and engineering design.

Tin-porphyrin sensitized TiO₂ for the production of H₂ under visible light

Kim, Wooyul,Tachikawa, Takashi,Majima, Tetsuro,Li, Changhong,Kim, Hee-Joon,Choi, Wonyong Royal Society of Chemistry 2010 ENERGY AND ENVIRONMENTAL SCIENCE Vol.3 No.11

<P>Hydrogen was successfully produced under visible light irradiation in a tin porphyrin (SnP)-sensitized TiO<SUB>2</SUB> system in the wide pH range (pH 3–11) although SnP hardly adsorbs on TiO<SUB>2</SUB>. The number of H<SUB>2</SUB> produced in the SnP/TiO<SUB>2</SUB> system after 9 h irradiation corresponds to the turnover number of 410. The apparent photonic efficiency for H<SUB>2</SUB> evolution was estimated to be 35% with the monochromatic radiation of 550 ± 10 nm. The photochemical production of hydrogen is mediated through the formation of the π-radical anion (SnP&z.rad;<SUP>−</SUP>) that subsequently transfers electron to TiO<SUB>2</SUB>. The photogenerated SnP&z.rad;<SUP>−</SUP> was monitored by transient absorption spectroscopy and its lifetime is long enough to survive the slow diffusion from the solution bulk to the TiO<SUB>2</SUB> surface, which makes the adsorption of SnP on TiO<SUB>2</SUB> not required for hydrogen production. This is clearly contrasted with the common ruthenium complex-sensitized TiO<SUB>2</SUB> system where the adsorption of the sensitizer complex is essentially required and the hydrogen production is limited to the acidic condition where the adsorption of the sensitizers on TiO<SUB>2</SUB> is allowed. The photocatalytic activity of SnP was mainly attributed to the Q-band (500–650 nm), not to the Soret band (420–430 nm) of which absorption intensity is much higher.</P> <P>Graphic Abstract</P><P>Hydrogen was successfully produced under visible light irradiation in tin porphyrin (SnP)-sensitized TiO<SUB>2</SUB> system in the wide pH range (pH 3–11) although SnP hardly adsorbs on TiO<SUB>2</SUB>. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0ee00205d'> </P>

Numerical analysis on scale effect of elasticity, strength and failure patterns of jointed rock masses

Peitao Wang,Tianhong Yang,Tao Xu,Meifeng Cai,Changhong Li 한국지질과학협의회 2016 Geosciences Journal Vol.20 No.4

It is of great importance to study the failure process and scale effect of jointed rock mass in the field of rock mechanics and mining engineering. In the present paper, initially the uniaxial compression test on granite was performed and acoustic emission (AE) sequence was acquired during the compression process in laboratory. Results from numerical simulations using the particle flow code in two dimensions (PFC2D) were presented, and compared with experimental measurements. It was observed that the approach was reasonably good in predicting the real response of granite rock samples. The mechanical parameter of joint model was then calibrated based on PFC2D model with experimental results. Finally the mechanical properties of complex rocks with discrete fracture network (DFN) were studied and scale effects on the elasticity and strength were then investigated. The result showed that the failure pattern was similar when the ratio of joint contact bond strength (both shear and normal) to rock contact bond strength was in the range of 3~9%. The elastic modulus and strength parameters were changed with the sizes of rock sample for DFN models. Moreover, the variation of rock failure pattern under different sizes was also studied and finally the representative elementary volume (REV) size of the considered rock mass was estimated to be 9 × 9 m. It is suggested that the failure pattern analysis should be considered in the REV study of jointed rock mass.