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Miaomiao Sheng,Huake Jia,Gongyou Zhang,Lina Zeng,Tingting Zhang,Yaohang Long,Jing Lan,Zuquan Hu,Zhu Zeng,Bing Wang,Hongmei Liu 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.5
Brevibacillus brevis GZDF3 is a gram-positive, plant growth-promoting rhizosphere bacterium (PGPR) isolated from the rhizosphere soil of Pinellia ternata (an important herb in traditional Chinese medicine). The GZDF3 strain produces certain active compounds, such as siderophores, which are the final metabolite products of non-ribosomal peptide synthetase (NRPS) and independent non-ribosomal peptide synthetase (NIS) activity. With the present study, we attempted to investigate the siderophore production characteristics and conditions of Bacillus sp. GZDF3. The antibacterial activity of the siderophores on pathogenic fungi was also investigated. Optimal conditions for the synthesis of siderophores were determined by single factor method, using sucrose 15 g/l, asparagine 2 g/l, 32°C, and 48 h. The optimized sucrose asparagine medium significantly increased the production of siderophores, from 27.09% to 54.99%. Moreover, the effects of different kinds of metal ions on siderophore production were explored here. We found that Fe3+ and Cu2+ significantly inhibited the synthesis of siderophores. The preliminary separation and purification of siderophores by immobilized-metal affinity chromatography (IMAC) provides strong antibacterial activity against Candida albicans. The synergistic effect of siderophores and amphotericin B was also demonstrated. Our results have shown that the GZDF3 strain could produce a large amount of siderophores with strong antagonistic activity, which is helpful in the development of new biological control agents.
Miaomiao Zhang,Wei Hong,Fangxi Xie,Yan Su,Liwei Han,Bin Wu 한국자동차공학회 2018 International journal of automotive technology Vol.19 No.3
A detailed investigation of the impact of injection timing and injection pressure on combustion and particles of a spray-guided GDI engine was conducted, under different engine operating conditions. The results indicated that, more proportion of large particles were emitted when increasing engine load, and the peak of accumulation mode particles moved toward smaller size when rising engine speed. With retarding the injection timing, the in-cylinder pressure and heat release rate rose first and then dropped at 2000 rpm, but they continuously rose at lower or higher speed conditions. The total particles concentration curves at all cases showed a trend of U-shape, and the corresponding timing of the lowest particles concentration advanced as the engine speed or load increased. The minimum value of emitted particles first rose and then fell when increasing load at 2000 rpm conditions, and it continuously rose when increasing speed at 40 Nm conditions. Generally, injection pressure did no sensitively affect combustion process except that it showed a relatively strong impact at low load conditions. However, particulate matter could be effectively inhibited by elevating fuel pressure from 5.5 to 11.5 MPa at all cases. In detail, the total particles concentration continuously fell at low speed and mid speed-high load cases, but it showed a rose trend when further increase fuel injection pressure at mid speed-low load and high-speed conditions.
Yingran Liang,Yuanyuan Zhang,Miaomiao Liu,Xue Han,Jianping Zhang,Xuan Zhang,Li Chu 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.8
Many studies describe the stimulating eff ect ofquercetin on Ca 2+ channels and the treatment of cardiovasculardiseases such as myocardial ischemia and hypertension. However, these studies are scattered and contradictory. The aim of this study is to elucidate the protective eff ects ofquercetin against isoproterenol (ISO)-induced myocardialischemia and verify the cellular mechanisms based on theL-type Ca 2+ channel (LTCC), Ca 2+ transients, and myocardialcontractility. An animal model of myocardial ischemiawas established by subcutaneous injection of ISO for 2 days. Quercetin significantly reduced J-point elevation, heartrate, reactive oxygen species, serum levels of myocardialenzymes, superoxide dismutase, catalase, glutathione, glutathioneperoxidase, glutathione S-transferase and improvedheart pathologic morphology. L-type Ca 2+ current (I Ca-L )was tested in an experiment with isolated rat myocardialcells by using the whole-cell patch-clamp recording techniqueand IonOptix Myocam detection system. Quercetin reduced I Ca-L in a concentration-dependent fashion with ahalf-maximal inhibitory concentration of 4.67 × 10 –4 M. Quercetin also shifted the current–voltage curve upwards,moved the activation and inactivation curves to the left andinhibited the amplitude of the cell shortening and Ca 2+ transients. The results showed that quercetin acts as a LTCCinhibitor and exerts a cardioprotective eff ect by inhibitingCa 2+ infl ux and contractility in rats.
Bacillus subtilis Spore Surface Display Technology: A Review of Its Development and Applications
( Guoyan Zhang ),( Yingfeng An ),( Hossain M. Zabed ),( Qi Guo ),( Miaomiao Yang ),( Jiao Yuan ),( Wen Li ),( Wenjin Sun ),( Xianghui Qi ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.2
Bacillus subtilis spore surface display (BSSD) technology is considered to be one of the most promising approaches for expressing heterologous proteins with high activity and stability. Currently, this technology is used for various purposes, such as the production of enzymes, oral vaccines, drugs and multimeric proteins, and the control of environmental pollution. This paper presents an overview of the latest developments in BSSD technology and its application in protein engineering. Finally, the major limitations of this technology and future directions for its research are discussed.
곽묘묘(Miaomiao Huo),전윤식(Yoonsik Jeon),유갑곤(Jiakun Liu),장홍연(Hongyan Zhang) 전북대학교 산업경제연구소 2018 아태경상저널 Vol.10 No.2
본 연구에서는 심층행위를 예측하는 데 있어 계획된 행위이론의 심층행위의도를 활용하였다. 심층행위는 감정을 조정하는 과정으로 심층행위의도와 심층행위의 관계에서 의도 안정성을 확보하기 어렵기 때문에 이러한 관계에서 개인이 쉽게 변할 수 없는 성격특성에 따라 영향관계의 강도가 변할 수 있다. 즉 본 연구에서 심층행위의도와 심층행위의 관계에서 우호성과 정서불안정성의 조절효과를 살펴보고자 한다. 또한 심층행위를 수행하는 과정에서 인구통계요인에 따라 어떤 차이가 있는지를 확인하는 것은 인적자원관리 합리성 제고에 의미가 있을 것이다. 연구결과에 따르면 조직에서 감정노동자들로 하여금 심층행위를 수행하면 심층행위의도를 유도할 필요가 있다. 또한 인사부서는 종업원들을 채용할 때 성격특성 측정을 통해 최대한 우호성이 강한 사람을 채용할 필요가 있고 정서불안정성이 강한 사람을 피할 필요가 있다. 마지막으로 인사부서에서 직원을 채용할 때, 여성 직원을 채용할 필요가 있고, 학력은 상대적으로 높은 사람을 채용할 필요가 있다고 볼 수 있다. In this study, we used deep acting intention to predict deep acting of emotional labor. Since deep acting is the process of adjusting emotion, it is difficult to insure the relationship stability between deep acting intention and deep acting, furthermore the relationship can be changed depending on personality characteristics. Hence, this study investigated moderating effects of agreeableness and instability between deep acting intention and deep acting. In addition, identifying differences in demographics in the process of conducting deep acting will be meaningful in raising human resource management rationality. This study suggests that HRM managers should focus on hiring people who with the highest level of agreeableness and avoiding people who with strong emotional instability. Furthermore, it is necessary to hire female employees and high education background employees.
Research on the Dynamic Scenes’ Ability to Adjust to the RFID Anti-Collision Calculation Method
Jie Zhang,Miaomiao Zhao 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.7
Anti-collision calculation method is an important technique in the RFID system. On the basis of the research of RFID’ s special decoding method and classic anti-collision method in a dynamic environment, using results of the ability of the reader to test the ability of the electronic tags to adjust itself to the blank time frames, controlling the reader’s region of communication and allowing the reader to be at its highest efficiency and decoding rate, combined with the binary tree calculation method, the ability of the tags to adjust to the binary tree anti-collision method. When the tags are adjusting to the dynamic environment, at the same time, the tags that are interfering with the process can be removed, improving the stability of the RFID signal. The results shows that as compared to the calculation method used now, this calculation method effectively controls the happening of collisions, increasing the efficiency of the RFID system’s reading of tags.
Research and Design of the Tobacco Machinery Equipment Remote Fault Diagnosis System Platform
Jie Zhang,Miaomiao Zhao 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.6
Because of the machine equipment fault has the fuzzy characteristics, based on the ZJ19E monitoring system, proposed one kind based on the Internet of things technology and fuzzy neural network intelligent fault diagnosis of remote control system scheme, established the fault diagnosis model based on fuzzy neural network, realizes the remote diagnosis of the machine equipment fault, improves the traditional control methods greatly improved productivity and automation, and improves the high rate of fault diagnosis of the machine equipment, makes the ZJ19E monitoring system remotable and intelligentialize.
Research on the Use of RFID Technology in Cigarette Production
Jie Zhang,Miaomiao Zhao 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.8
With regard to the cigarette manufacturing’s demand for modern management, to protect the integrity and reliability of the cigarette production processing data, the proposed RFID application in cigarette production system, building on the basis of RFID security controlled digital information system platform, the system covers the entire process of cigarette production and can realize the control and management of cigarette production and optimize the improvement on the basis of binary ALOHA anti-collision algorithm. It can also improve RFID’s recognition accuracy to achieve the basic information for cigarette products in the process of digitization accuracy.