苦蔘이 家免의 血管收縮抑制에 미치는 影響

권오율,남창규,정찬길 世明대학교 한의학연구소 1999 韓醫學硏究所 論文集 Vol.2 No.-

The purpose of this study was to analyze the effect and mechanism of Sophorae Radix on arterial experimental section contracted by PE(phenyl-ephrine). The results were summarized as follows; 1.Sophorae Radix significantly ingibited the PE-induced contraction of artery, accordingly as concentration of Sophorae Radix increased. 2.Sophorae Radix significantly ingibited the PE-induced contraction of artery both with intact endothelium and removed endothelium. 3.The ingibiting effect of Sophorae Radix on contracted strips was not changed by the pretreatment of NLA(Nω-nitro-L-arginine). 4.Sophorae Radix more significantly inhibited the PE-induced contraction of artery than KCI-induced. 5.The mechanism of inhibition of Sophorae Radix on contracted strips is suggested that Ca++ was blocked from the external medium.

제주도 토양 방선균의 속 다양성 분포

권오성,박동진,이찬용,김창진 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

제주도 전역에서 채집한 59점의 토양시료로부터 총 938주의 방선균을 분리하였다. 분리된 각 균주의 형태적, 생리적 특성을 조사하고 이를 기준으로 속명을 동정하므로써 제주도 지역 토양 방선균의 속 다양성을 비교하였다. 그 결과 Streptomyces 속 방선균이 62.6%를 차지하였으며 Micromonospora 속은 16.4%, Nocardioform 군은 8.6%, Actinomadura 속은 2.2%, Microbispora 속은 1.0% 나머지 Streptosporangium 속은 5.9%이었다. 토양 환경별로 보면, 초지에는 Streptomyces 속과 Micromonospora 속 균주가 풍부하였고, 밭에는 Streptomyces 속과 Micromonospora 속 균주가 풍부하였고, 산림에는 Micromonospora 속과 기타 속 균주가 풍부하였으며 기타 지역과 더불어 방선균의 속 다양성이 풍부한 편이었다. 그리고 특히 자연 동굴 토양에서는 Streptomyces 속의 분포 비율이 높지 않았으며 상대적으로 Nocardioform 균주의 분포 비율이 특히 높았다. Total 938 actinomyete strains were isolated from 58 soil samples collected at Cheju island. All of these isolates were identified to the genus level based on morphological and physiological characteristics. As the result, 62.6% of those isolates were Streptomyces, 16.4% were Micromonospora, 8.6% were Nocardioform group, 2.2% were Actinomadura, 1.7% were Microbispora, 1.6% were Nocardiopsis, 1.0% were Streptosporangium, and 5.9% were the others. As the sources of soil, Streptomyces and Microbispora were abundant in grassland soil, Streptomyces and Micromonospora were abundant in field soil, and Micromonospora were abundant in forest soil. Especially, Nocardioform strains were abundant in natural caves.

리벳 구멍을 가진 알루미늄 박판구조의 피로손상 탐지를 위한 음향방출의 활용

김정찬,권오양,김성진 한국비파괴검사학회 2003 한국비파괴검사학회지 Vol.23 No.3

항공기 구조를 모사하여 일련의 리벳 구멍을 갖는 AA2024-T3 박판 구조를 대상으로 피로하중에 의한단균열(short crack)의 발생시점과 성장거동을 음향방출(AE)을 위주로 한 측정으로 평가하였다.AE 위치표정에 의해 단균열의 좌표를 정확하게 결정하였으며, 이동식 현미경으로 균열의 크기를 측정하였다. 누적 AE 발생수 곡선은 단균열의 발생과 성장에 따라 일정한 간격을 두고 급격히 증가하는 양상을 보임으로써 여러 차례의 계단식 곡선을 형성하였다. AE 위치표정에서는 리벳구멍을 중심으로 파괴역학에 근거한 관심영역(ROI)을 설정하였으며, 웨이블릿변환 잡음제거 방법을 사용하여 위치표정의 정확도를 향상할 수 있었다. 실제로 탐지된 신호의 대부분이 단균열의 발생 및 성장과 관계없는 외부 잡음신호로 나타났으며, ROI 내에서 발생한 AE 발생원의 위치도 구조의 기하학적 특징이나 신호대잡음비의 영향에 의해 왜곡될 수 있음을 알 수 있었다. The initiation and growth of short fatigue cracks in the simulated aircraft structure with a series of rivet holes was detected by acoustic emission (AE). The location and the size of short cracks were determined by AE source location techniques and the measurement with traveling microscope. AE events increased intermittently with the initiation and growth of short cracks to form a stepwise increment curve of cumulative AE events. For the precise determination of AE source locations, a region-of-interest(ROI) was set around the rivet holes based on the plastic zone size in fracture mechanics. Since the signal-to-noise(SNR) was very low at this early stage of fatigue cracks, the accuracy of source location was also enhanced by the wavelet transform de-noising. In practice, the majority of AE signals detected within the ROI appeared to be noise from various origins. The results showed that the effect of structural geometry and SNR should be closely taken into consideration for the accurate evaluation of fatigue damage in the structure.

2,4-Dinitrophenol을 처리한 호밀(Secale cereale L.) 유식물의 질산염 환원에 관한 연구 : Ⅰ. 호밀 유식물 묘조의 질산염 환원요소 활량에 대한 2, 4-Dinitrophenol의 영향 Ⅰ. Effect of 2, 4-Dinitrophenol on in vivo Nitrate Reductase Activity in the Shoots of Rye Seedlings

曺圭燐,李相淑,權五溶 충남대학교 1991 忠南科學硏究誌 Vol.18 No.1

This work was accomplished to determine the effect of 2,4-dinitrophenol (DNP) on in vivo nitrate reductase activities(NRA) in the shoots of 6 day old rye(Secale cereale L.) seedlings. The NRA in the shoots of rye seedlings pretreated with 0.5mM DNP was higher than that of the control group in all the experimental conditions as below. The optimal concentration of KNO_3 for maximum NRA was 10mM in both the control and treated group. The NRA accumulated with the treatment of 10mM KNO_3 was gradually increased for 4 hours in both groups, and then maintained constantly. On the other hand, the NRA occurred per hour was highest at 1 hour in both groups, while it was declined subsequently. The optimal pH for NRA was 7.5 in both groups. The daily pattern of NRA was markedly decreased for 4 days in both groups, and decreased slowly to 7 days. The NRA was greatly inhibited in the solutions of 200mM KCI and NaCl. According to the treatment time with the solutions of 200mM KCI and NaCl, the NRA was extremely inhibited at 2 hours, thereafter recovered slowly from its inhibition effects.

항공기구조 모사 알루미늄 박판에서의 피로균열 탐지 및 표면초음파를 이용한 균열크기 측정

김정찬,권오양 한국비파괴검사학회 2004 학술대회 논문집 Vol.- No.-

Since the fatigue damage usually occurs around the rivet holes in aircraft structures, the detection and sizing of short fatigue cracks emanating from rivet holes is extremely important. The initiation of fatigue cracks in a simulated aircraft structures with a series of rivet holes was detected by acoustic emission(AE), and the crack length was determined by the surface acoustic wave(SAW) technique. AE events increased intermittently with the initiation and growth of short cracks to form a stepwise incremental curve of cumulative AE events. with the SAW technique employed, the crack sizing in the range of 1∼8 mm long was possible but it was impossible in the range shorter than 1 mm.

폴리머를 이용한 하수관로에서의 퇴적물 제거 기법

전찬열,오율권,황세준 한국산업안전학회 2002 한국안전학회지 Vol.17 No.4

This experimental study is aimed to increasing the velocity in the sewerage and also development of easy removal method for a corrosion and sand in the flow pipe. And the result by the increasing the our flow per unit time, diameter of sewerage pipe is becoming small size, cut down the construction fee and the same time reducing the tubulent flow pattern in the flow pipe. As a result of detected experimental velocity signal from the probe in the test wall surface, reducing the pump power and increasing the out flow per unit time. Apparent water velocity decreases as the value of void ratio increases depends on the varies center of pipe.

웨이블릿 변환 노이즈 제거에 의한 AE 위치표정

이경주,권오양,주영찬 한국비파괴검사학회 2000 한국비파괴검사학회지 Vol.20 No.6

사용하는 탄성파의 파장과 두께가 비슷하거나 보다 얇은 박판 구조에서 음향방출(acoustic emission, AE)신호의 위치표정 정확도의 향상을 위해 새로운 신호처리 방법인 웨이블릿 변환 디노이징(wavelet transform de-noising) 기법을 도입하였다. 탐지된 AE 신호에 대하여 웨이블릿 변환과 역변환을 수행하여 상대적으로 저주파수이고 큰 진폭을 갖는 굽힘과 성분(flexural component)은 활용하고, 고주파수이고 작은 진폭의 팽창파 성분(extensional component)은 필터링하여 제거한 다음 신호를 재구성하는 디노이징 처리를 거침으로써 박판에서의 위치표정 시 발생하는 도달시간차 측정오차를 최소화할 수 있음을 확인하였다. 따라서 웨이블릿 디노이징 처리를 도입함으로써 위치표정의 정확도가 게인(gain)이나 문턱값의 설정, 판의 두께, 센서간거리, 발생원과 센서의 상대적인 위치에 무관하고 전통적인 문턱값 등과 방법에 비하여 월등하게 향상되었다. 또한 상대적으로 매우 큰 진폭을 가지는 굽힘과 성분을 활용하므로 실제적인 박판 구조물에서의 위치표정에 효과적으로 활용될 수 있을 것이다. A new technique for the source location of acoustic emission (AE) in plates whose thichness are close to or thinner than the wavelength has been studied by introducing wavelet transform de-aoising technique. The detected AE signals were pre-prossed using wavelet transform to be decomposed into the low-frenquency, high=amplitude flexural components and the high-frequency, low-amplitude extensional components. If the wavelet transform de-noising was employed, we could successfully filter out the extensional wave component, one of the critical errors of source location in plates by arrival time difference method. The accuracy of source location appeared to be significantly improved and independent of the setting of gain and threshold, plate thickness, sensor-to-sensor distance, and the relative position of source to sensors, Since the method utilizes the flexural component of relatively high amplitude, it could be applied to very large, thin-walled structures in practice.

Cantharis의 세포독성

殷載淳,權鎭,全焄,吳贊鎬,蘇俊魯 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

The cytotoxicity of cantharidine(CTD), the main component of Cantharis, and the combined effect of CTD and anti-tumor drugs on HeLa. Hep G2,SK-OV3. KOHS-NP, BALB/c 3T3 cells, mouse splenocytes and human lymphocytes were estiated by MTT colorimetry assay. CTD inhibited the proliferation of anti-tumor cells, BALB/C 3T3, mouse spleen cells and human lymphocytes. CTD increased the cytotoxicity of mitomycin C, cisplatin or mercaptopurine on tumor cells and BALB/c 3T3 cells. These results indicate that cantharidine has the cytotoxicity of tumor cells, fibroblast cells and immunocytes.

쌍자엽식물의 자엽에 있어서 α-Amylase활성에 관한 식물생장물질의 연구 : Ⅰ. 녹두 자엽에서 α-Amylase 활성에 대한 식물 생장 조절체의 효과 Ⅰ. Effects of Plant Growth Regulator on α-Amylase Acticity in the Cotyledons of Mung Bean(Phaseolus radiatus L.)

李相淑,曺圭燦,權五溶 충남대학교 자연과학연구소 1992 忠南科學硏究誌 Vol.19 No.1

The effects of plant growth regulators on α-amylase activities in the embryonic axis-attached and detached cotyledons of mung bean (Phaseolus radiatus L.) have been investigated. α-Amylase activities in the attached cotyledons of developing mung bean have been markedly increased for 3 days after the imbibition and then gradually decreased. The changes of α-amylase activity have accorded with changes on the band density of α-amylase isozymes in the attached and detached cotyledons. Two bands of isozyme have appeared on day 3 in the attached cotyledons, but on day 4 in the detached cotyledons. When the 10^-6 M solutions of GA_3 and kinetin have been exogenously supplied in the detached cotyledons for 3 days, α-amylase activities have occurred with about 1.5-fold in GA_3 and 1.25-fold in kinetin rather than the control. α-Amylase activities have been increased with 1.8-fold in the detached cotyledons treated with the 10^-3 M and 10^-4 M solutions of IAA. In contrast with the treatment of IAA, α-amylase activities have been increased with 3.5-fold in the detached cotyledons treated with the 10^-5 M and 10^-6 M solutions of 2, 4-dichlorophenoxyacetic acid belonged to synthetic auxin. Consequently, it has been indicated that the embryonic axis in the cotyledons of mung bean has been directly influence on α-amylase activity, and the α-amylase activity of the detached cotyledons treated with a 10^-6 M solution of 2, 4-dichlorophenoxyacetic acid has developed much increased effect. For this reason, it has been suggested that auxin from the embryonic axis has been probably induced α-amylase in the attached cotyledons during germination of mung bean.

L1210 세포증식에 대한 Glycyrrhizin의 억제작용 기전

殷載淳,徐龍勳,權鎭,柳東和,吳贊鎬,蘇俊魯,全焄,黃甲洙 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

The purpose of this research was to investigate the mechanism of inhibitory action of Glycyrrhizin(GZ) on the proliferation of mouse leukemia cell-line, L1210 cells. The cytotoxic activity was tested using a colorimetric tertrazolium assay(MTT assay), the apoptosis was tested using flow cytometry. Nitric oxide(NO) production form mouse peritoneal macrophage was tested using a Griess method and the phagocytosis of human polymorphonuclear cells was tested using a lucigenin chemiluminescence. GZ ingibited the proliferation of L1210, BALB/c 3T3 cells and mouse thymocytes at 50 ug/ml/ GZ did not affect nitric oxide production from mouse peritoneal macrophages in vitro, but ingibited nitric oxide production from lipopolysaccharide and y-interferon treated macrophages. Macrophages of GZ-administered mice accelerated NO production. The proliferation of L1210 cells apoptosis of L1210 cells were induced by co-culture with macrophage of GZ-administered mice. The apoptosis of L1210 cells were induced by co-culture with macrophage of GZ-administered mice. GZ increased the phagocytosis of human polymorphonuclear cells. These results suggest that GZ inhibit the proliferation of L1210 cells not only as a direct cytotoxic agent o tumor cells, but also by the enhancement of NO production and phagocytic activity.