모세관 전기 영동법에 의한 분석에서 피크 모양 및 검출 향상을 위한 단백질의 유도체화

조병연 경희대학교 자연과학종합연구원 2000 자연과학논문집 Vol.6 No.-

단백질 및 항체를 6-aminoquinolyl-N-hydroxysuccinimidyl (6-AQC) 시약을 사용하여 단일 피크가 생성되는 조건으로 유도체화 하였다. 유도체화를 통하여, 전기 영동법에 의한 분석에서 피크 모양 및 검출 한계 등이 향상되었으며, pI 값 등의 변화 등이 관측되었다. 이러한 실험 결과 단백질의 전기 영동법에 의한 분석에서 더 좋은 분리도 및 피크 확인이 가능하였다. 유도체화된 단백질은 항원-항체 반응을 통하여 활성도를 확인하였으며, 단백질에 따라 활성도를 유지하는 단백질이 관측되었다. Proteins and antibodies can be derivatized with 6-ammoqumoly l-N- hydroxysuccinimidyl (6-AQC) reagent, leading to a single HPCE peak. These species have greatly improved peak shape and performance, improved detectabilities, altered pI values, and in general, improved identification properties in various HPCE modes. The confirmation of the activity of the derivatized proteins through antibody-antigen recognition was also performed, and the activity of some tagged proteins was observed.

인터넷의 효과적 활용을 위한 수업모형 개발 연구

조주연,이병승 서울敎育大學校 初等敎育硏究所 2002 한국초등교육 Vol.13 No.2

The purpose of this study was to develop a teaching model for elementary schools which takes advatage of the internet. For the purpose, this study carried out aalyzing ad synthesizing the internet-based teaching models which had been developed by other researchers. This study has especially operated an internet cafe for elementary classroom students/teacher/parents as an experiment of this study. As a result, this study identified and analyzed two internet-based teaching models; (1) Resource-centered teaching model and (2) Exploratory project teaching model. The Resource-centered teaching model is a teaching model in which the teacher makes a search for instruction-related materials and presents students the materials, when it is possible to access the internet in the classroom. The Exploratory project teaching model is a teaching model in which students themselves search for learning materials according to the subject. This study established a classroom cafe(www.daum.net/ayo51) in the internet, and let the classroom members participate in the bulletin board, data room and/or chatting room. The teacher's diary and classroom diary let the teacher and students exchange emotions with one another. Students' parents also participated in the cafe, asking questions or discussing with the teacher. The processes and results of this study let the co-researchers reach the following conclusions. First, educators in the elementary education have to make a rational/systematic approach to take advantage of the internet materials for students. Second, the teacher has to think of the internet as another effective method of the school education, and be active in using the internet during his/her teaching. Third, educators have to develop continuously the contents for the internet classroom which are appropriate to students' intellectual developments and their learning circumstances.

林道의 迂廻係數 算出 : 江陵營林署의 國有林道를 대상으로 National forest roads of Kangnung national forest office

曺丘鉉,池炳潤,車斗松 江源大學校 林科大學 森林科學硏究所 1994 Journal of Forest Science Vol.10 No.-

본 연구는 林道開設을 위한 기초자료로서 江陵營林署의 5개 管理所(강릉, 양양, 삼척, 정선, 평창관리소)의 國有林道를 대상으로 傾斜別 林道迂廻係數를 산출하였다. 그 결과, 林道迂廻係數는 경사도 5%이하에서 0.33, 6∼10%에서 0.39, 11∼15%에서 0.50, 16∼20%에서 0.58, 및 21%이상에서는 0.63으로 나타났다. This study was carried out to estimate forest road elongation coefficients for providing the basic information of forest road construction. As the results, road elongation coefficients were calculated 0.33 less than 5% of road gradient, 0.39 between the gradient of 6% and 10%, 0.50 between the gradient of 11% and 15%, 0.58 between the gradient of 16% and 20%, and 0.60 greater than the gradient of 20%.

직업성질환 감시체계의 계획과 관리

조수헌,홍윤철,임종한,장성실,천병철 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.1

직업성 질환에 관련된 다양한 진료를 체계적으로 수집하고 관리하여 직업성질환의 예방 목적으로 이를 활용하고 자하는 노력이 우리나라에서도 이제 다양한 방법으로 시도되고 있다. 최근의 자료 시스템의 구축과 관리 방법의 향상은 새로운 직업성 잘환감시방법을 개발하게 하여 직업성질환과 손상을 예 방하는데 크게 기여하고 있다. 우리나라에서도 기존의 산업보건사업의 성과를 계승하면서, 외국의 직업성질환 감시의 여러 경험을 비판적으로 수용하여 한국적인 직업성질환 감시 모델을 구축해야 할 것으로 생각된다. 자발적인 지역차원의 직업성질환 감시체계들이 틀을 잡아가고 있는 이 시점에서 본격적으로 직업성질환 감시체계 수립을 위한 전략과 구체적 방법론을 마련하고 공유하여야 할 필요가 있다. 이를 위하여 우리나라의 직업성질환 감시체계를 구축하는데 기초가 될 수 있는 직업성질환 감시체계의 계획과 관리에 대한 내용을 정리하였다. 이러한 감시체계를 우리나라에 정립하려는 노력들의 일환으로 최근 직업성질환감시연구회의 결성 및 각 지역의 직업성질환 감시체계의 운영 등이 조금씩 결실을 맺어가고 있어 간략히 소개하면 다음과 같다.첫째, 최근에 인천, 천안, 대전, 여천, 구미 등에서 지역단위의 직업성질환 감시체계를 구축하고 있고 한국산업안전공단에서는 특수건강진단과 작업환경측정 자료를 전산화하여 이를 직업성질환 감시에 사용하는 전국단위의 감시체계와 천식, 근골격계질환, 피부질환 등 질환별 감시체계 등이 시도되고 있다.둘째, 이러한 움직임은 과거에 비하여 한층 발전된 양상이고 바람직하지만 이제는 국가적 차원에서 직업성질환 감시체계를 어떻게 설정하고 조율할 지를 고민하고 계획하여야 할 때이다. 즉 한국산업안전공단과 지역단위 직업성질환 감시체계들의 역할분담과 상호 정보의 교류 등이 정리되어야 이를 바탕으로 유기적인 국가 직업성질환 감시체계가 구축될 수 있다.셋째, 이를 위하여서는 직업성질환 감시체계 구축의 방법론을 서로 교류하고 공유할 필요가 있으며 나아가서는 이를 표준화하여 기본적인 자료의 교류가 원활하게 이루어질 수 있도록 하여야 한다. 이러한 역할은 최근에 결성된 직업성질환감시연구회와 산업안전보건연구원, 그리고 대한산업의학회, 산업간호학회, 산업위생학회 등이 협력하여 학술적인 작업과 함께 실무적인 지침을 만들어나가도록 하는 것이 바람직할 것이다.

돼지에서 정맥, 근육 그리고 경구 투여시의 enrofloxacin의 약물동태학

윤효인,김무열,박승춘,조준형,박병권,이내경,노상석,장범수,신광순,조명행 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

In order to characterize pharmacokinetic profiles according to route of a new enrofloxacin salt form (Enrotil®), it was given to 4 healthy pigs via oral (p.o.), intramuscular (i.m.) or intravenous (i.v.) administrations at a dose rate of 5 ㎎/㎏ body weight. Enrofloxacin (ENFX) in serum was detected by bioassay using E. coli BE1186 as a test organism. The biological elimination half-lives (t_1/2(β)) of ENFX were 6.76±0.99 h (i.v.), 7.16±2.30 h (i.m.) and 11.45±3.90 h (p.o.), Volume of distribution (Vd) of enrofloxacin was 2.20±0.31 L/㎏ (i.v.), 2.52±0.60 L/㎏ (i.m.) and 1.88±0.33 L/㎏ (i.m.). Mean residence time (MRT) was 8.77±1.26 h after i.v. injection and the maximal concentration time (Tmax) following p.o. and i.m. administration was 0.76±0.09 h and 0.60±0.12 h, indicating a rapid absorption from these routes. Bioavailibility (F) was calculated as 64.1% for p.o. administration and 59.71% for i.m. injections. In summary, the newly formulated enrofloxacin salt form has shown a high water solubility, rapid absorption and large tissue distribution, suggesting a potential antibacterials for oral application on a large scale in veterinary sectors.

DSM-Ⅲ-R 성격장애 진단의 내용타당도

양병환,조연규,김창현,황순택,오동열 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.2

The purpose of this study was to examine the content validity of DSM-Ⅲ-R personality disorder(PDs). A 3-point rating scale of 150 preliminary diagnostic criteria including 95 DSM-Ⅲ-R PD diagnostic criteria was sent to psychiatrists. Each item in this scale was rated by psychiatrists(n=101) in term of prototypicality to every DSM-Ⅲ-R PD. The results show that each PD criteria set of DSM-Ⅲ-R was registered high level of interrater consistency and items listed in the DSM-Ⅲ-R were, for the most part, rated as very prototypical to their DSM-Ⅲ-R diagnosis. Some criteria were, however, not rated to characteristic features to their DSM-Ⅲ-R diagnosis. 11 items of DSM-Ⅲ-R diagnostic criteria scored high not only to their DSM-Ⅲ-R diagnosis but also other diagnosis. 16 items rated as high prototypicality item of other diagnosis. Another 16 items seemed not to be appropriate to any of DSM-Ⅲ-R PD diagnosis. These results suggest that the content validity of the DSM-Ⅲ-R personality disorder is not so high.

FET형 반도체 마이크로센서 개발(Ⅱ)

손병기,이흥락,박이순,조진호,이성필,최평,서화일,고성택,박재윤,서장수,김창수 경북대학교 센서기술연구소 1994 연차보고서 Vol.1994 No.-

현재 의료진단, 화학공정의 모니터링이나 환경공학적 감시 및 제어 등의 분야에서 사용되고 있는 기존의 센서는 고가이며 용적이 클 뿐만 아니라 분석시간이 길고 사용하기 까다로운 것 등 여러 가지 문제점이 있다. 또한 측정환경에 영향을 주지 않을 만큼 충분히 작으며 빠른 분석시간을 가진 센서를 필요로 하고 있다. 본 연구에서는 기존의 센서들의 난점을 극복할 수 있는 새로운 형태의 FET형 전해질(electrolyte : H^+, K^+, Ca^2+, Na^+)분석 시스템, 용존가스(O_2, CO_2)분석 시스템의 개발을 중점적으로 추진하며, FET형 압력센서, 습도센서, 방사형 온도센서 등의 개별 FET형 센서에 관한 연구도 병행하고자 한다. Recently various kinds of sensors have been developed, being applicated to their own purpose. There are lots of difficulties to apply them to measurements in which the real-time monitoring is required without disturbing the surrounding environment. FET type semiconductor sensors, fabricated by the semiconductor integration technologies, have many advantages for their miniaturization, standardization, mass-production and in vivo/in situ monitoring. They also hold a very proper configuration for multi-functional sensors or integrated smart sensors, and wide availability by forming various kinds of physical or chemical sensing materials onto their sensing gates.

승모판 대치술후에 발생한 좌심실유출로 폐색 1예

김도연,조홍근,정익모,박시훈,박성훈,신길자,정병철 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.1

산업용 인버터의 모니터링을 위한 내장형 리눅스 적용

최병욱,조덕연 선문대학교 2001 공학계열 논총 Vol.4 No.-

In this paper, we apply the embedded Linux to monitor the status of industrial inverter, which is for controlling the motor of hydraulic elevator. And we implement the monitoring board using an embedded web server in which embedded Linux is running inside. The monitoring board is based on ARM7TDMI and has Ethernet controller to connect internet. The web-based monitoring system using embedded Linux can reduce the cost, and have flexibility both of technical issues and locations of the system to be monitored. The system shows the feasibility of remote monitoring system based on embedded web server running as a daemon in embedded Linux.

사과의 열과원인에 관한 조사연구

권병규,강영호,이우승,성민웅,이상윤,조화석 慶北大學校 生産技術硏究所 1970 生産技術 Vol.4 No.-

It is very important to eliminate the immense economic loss caused by cracking in apples through examining the causes of cracking and divising a possible method of prevention of it. Several investigators published their findings on the causes of the cracking of apples which develop on the skin of Ralls apples. However, no definite conclusions or established methods of prevention have been published to this date. Therefore, in this study the causes of the cracking of apples are examined through the use of several methods: (a) the histological and physiological, (b) the microelemental, and (c) the natural environmental. The results are as follows: 1). At the early stage of growth, the thicknesses of the cuticle, as well as of the subepidermis, were the same in Ralls apples without envelopes, Ralls with envelopes, and Jonathan. However. at maturity. the thicknesses differed in the following manner; Ralls apples without envelopes were thickest: Ralls envelopes were less thick than those without envelopes; while Jonathan apples were thinest. 2). Although in the Ralls without envelopes the epidermis is arranged in a brick wall like layer during maturity, in the middle of August the brick wall like epidermis begins to collapse and is surrounded by the cuticle. The process of cracking seems to start with the lenticle and the fruit spot. 3). The rate of cracking was slowed by application of a mixture of IAA and Bordeaux mixture solution, but cracking was not completely halted by this treatment. 4). The stem of a branch laden with apples was put into a solution containing Mn-54, and after absorption took place the distribution of Mn-54 was measured. The results of the measurements were as follows; the bark contained the most Mn-54; the leaves contained the second largest quantitiy; and the apples has the least. 5). The two orchards which were tested tended to have a low soil pH, and a high concentration of soil-solution Mn. 6). The soil with a high rate of cracking tended to have a high pH, which the soil with a low rate had a low pH. 7). In trees with a high rate of cracking, the amount of Mn in the leaves was equal to the amount in the stalk. However, in trees with a low rate of cracking, the amount of Mn in the leaves tended to be high compared with that found in the stalk. 8). The epidermis of the Ralls had a high Ca content than the epidermis of the Johnathan. 9). The epidermis of cracked apples tended to have a higher content of Ca, K,P, Cu and Fe than the epidermis of sound apples. 10). Amoug the apples tested, 11% cracked during the course of the year, with the cracking starting in the middle of Sept. 1969, This low percentage of cracking can be attributed to the small amount of rain during harvest time (late Sept. and October). The total of the added temperature during May, June, and July was 1962℃. 11). Cracking occurred more frequently in apples on the periphery of the tree, in well-colored apples, on the south side of the apples, and on the cheeks of the apples. These occurrances can perhaps be attributed to the amount of sunlight. 12). In the trees tested with newspaper envelops, cracking occurred in 1.9% of the apples with envelopes, and in 11.9% of the apples without envelopes. In the trees tested with Polysteron paper envelopes, cracking occurred in 2.5% of the apples with envelops, and 13.4% of the apples without envelopes. The apples with Polysteron paper envelopes were less infected by insects than the apples with newspaper envelopes. 13). Cracking occurred in 2.4% of the apples covered with vinyl, while in trees not covered with vinyl, 16.6% of the apples crack. The Artificial precipitation produced during harvest time increased the rate of cracking.