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Yoon, Cheol-Hee,Jang, Dai-Ho,Kim, Kyung-Chang,Park, Soon Young,Kim, Hye-young,Kim, Sung Soon,Chi, Sung-Gil,Choi, Byeong-Sun S.Karger 2014 Intervirology Vol.57 No.2
<P>Abstract</P><P><B><I>Objectives:</I></B> Persistent HIV-1 infections are characterized by a long silent infection period in resting CD4+ T cells, which allows them to escape the host immune response. Several HIV-1 latency mechanisms have been reported, but the molecular mechanism underlying polycomb repressor complex (PRC)-mediated HIV-1 latency remains poorly understood. <B><I>Methods:</I></B> Expression of PRC proteins in latent cells was measured by Western blot. Knockdowns of PRC genes were conducted by the specific siRNA and methylations at H3K27 on the proviral LTR were investigated by ChIP assay. <B><I>Results:</I></B> PRC proteins (EED, BMI-1, and RNF2) were dramatically downregulated in latent cells after PMA treatment. The downregulation of PRC proteins was followed by a decrease in the methylation of H3K27 and ubiquitination of H2AK119 in the PMA-treated latent cells. siRNA knockdowns of EED and BMI-1 also enhanced HIV-1 reactivation significantly in latently infected cells. By contrast, proteasomal inhibitor MG132 successfully abrogated the PMA-induced downregulation of PRCs. In particular, di-/tri-methylations of histone-3 in the proviral LTR was absent from latent cells after PMA treatment. <B><I>Conclusions:</I></B> This study shows that PRC is strongly related to the control of HIV-1 latency and that PRC-breaking agents may be helpful for purging HIV-1 from latent reservoirs.</P><P>© 2014 S. Karger AG, Basel</P>
군소(Aplysia kurodai)에 분포하는 글루코사미노글리칸의 추출과 기능특성 : 1. 다당류 추출의 최적화와 글루코사미노글리칸의 정제
윤보영(Bo-Yeong Yoon),최병대(Byeong Dai Choi),최영준(Yeung Joon Choi) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.11
남해안에서 주로 여름철에 어획하여 부산 및 경남 지역을 위주로 소비하고 있는 군소에서 다당류 추출을 위한 최적조건을 검토하였다. 군소 다당류 추출을 위한 단백질 가수분해 효소로는 Flavourzyme 500 MG가 가장 효과적이었고, 추출한 다당류의 회수를 위한 가장 효율적인 추출물의 농도와 ethanol 첨가량은 각각 Brix 60과 5배량이었다. DEAESepharose 칼럼 크로마토그래피로 얻은 크로마토그램상의 전기전도도와 전기영동 이동도에 근거하여 0.5~0.75 M NaCl 상에서 용출되는 물질이 GAG임을 확인하였으며, heparan sulfate인 것으로 추정하였다. 다당추출물과 정제 GAG의 uronic acid 함량은 각각 1.0 g/100 g과 6.0 g/100 g이었고, GAG를 구성하는 성분으로 지표물질인 hexosamine의 함량은 당 추출물과 정제물에서 각각 5.6 g/100 g과 25.7 g/100g이었다. 정제물은 agarose 겔 전기영동 상에서 heparan sulfate로 추정되었으며, 분자량은 겔 크로마토그래피에서 29.6 kDa으로 나타났다. The optimum condition was investigated for the extraction of glycosaminoglycan (GAG) from sea hare, Aplysia kurodai. The most effective enzyme was Flavourzyme for extraction of glycosaminoglycan. The optimum incubation temperature and time for hydrolysis were 60℃ and 15 hr, respectively. The yield of precipitated polysaccharide depended on Brix and ethanol volume. The most effective concentration of Brix and ethanol were sixty and 5 volume of ethanol, respectively. Most GAG was eluted between 0.5 M and 0.75 M NaCl gradient on DEAE-Sepharose column, and identified by electroconductivity. The contents of hexuronic acid from polysaccharide extract and GAG were 1.0 g/100 g and 6.0 g/100 g, respectively. Hexosamine of polysaccharide and GAG as indicator of GAG component was 5.6 g/100 g and 25.7 g/100 g, respectively. GAG was identified as heparan sulfate compared with bands of other GAG on agarose gel electrophoresis, and its molecular weight was 29.6 kDa on Superdex 200 HR column.
이명갑,김성진,최대해,전덕호,유병대,이동필 대한응급의학회 2002 대한응급의학회지 Vol.13 No.4
Purpose: There has been an increase in the number of nontraumatic prehospital cardiac arrests due to increases in both cardiovascular diseases and the average age of the population. We performed this study to identify the proper resuscitation technique to be used to increase the survival rate in nontraumatic, prehospital cardiac arrest. Methods: We reviewed the charts of patients with nontraumatic, prehospital cardiac arrest who visited our Emergency Department of Keimyung University Hospital during the period of May 2001 through April 2002. Results: Out of 60 cases of nontraumatic, prehospital cardiac arrest, 27 (45%) experienced no ROCS, 15 (25%) experienced transient ROSC, 6 (10%) died after 24 hours, and 12 (20%) alived and were discharged. Among those discharged, 10 had visited our emergency department for cardiac causes and 2 for non-cardiac causes. EKG findings were VF in 8, PEA in 2, and bradycardia in 2. In the discharged survival cases, the cardiac arrest had been witnessed. Conclusion: We failed to find significant statistical survival differences based on the causes of cardiac arrest, the initial EKG monitoring, or the method of visit. Witnessed cases of cardiac arrest had a higher survival rate than non-witnessed cases (p<0.05).
산업장 화학 사고로 응급실에 내원한 환자의 임상적 고찰: 일개병원의 후향적 연구
신희준 ( Hee Jun Shin ),오세광 ( Se Kwang Oh ),유병대 ( Byeong Dai Yoo ),전덕호 ( Duck Ho Jun ),이동하 ( Dong Ha Lee ),우극현 ( Kuck Hyeun Woo ),윤성용 ( Seong Yong Yoon ),최성용 ( Sung Yong Choi ) 대한임상독성학회 2015 대한임상독성학회지 Vol.13 No.2
Purpose: The purpose of this study is to understand what kinds of chemical substances have been used annually and to investigate incidents that occurred due to chemical hazard release and to analyze statistically clinically chemical injury patients who visited one regional emergency medical center in Gumi city with documented references review. Methods: Annual chemical waste emission quantity (Kg/Year) (Cwep) was reproduced using national web site data governed by the Ministry of Environment and 5 years (from 1 .Jan. 2010 to 31. Dec. 2014) of medical records of chemical injury patients who visited our emergency department were reviewed retrospectively. By applying exclusion criteria, 446 patients of 460 patients were selected. Results: Dichloromethane, Toluene, Trichloroethylene, and Xylene were always included within Top 5 of Cweq. Six cases of chemical incidents were reported and in 3 of 6 cases involving Hydrogen fluoride were included during the study period. Male gender and twenties were the most prevalent group. Injury evoking chemicals were Hydrogen fluoride, unknown, complex chemicals (over 2 substances) in sequence. The most frequent site of wounds and injuries was the respiratory tract. Gas among status, intoxication among diagnosis, and discharge among disposition was most numerous in each group. Conclusion: There have been no uniform clinical protocols for chemical wounds and injuries due to various kinds of chemicophysical properties and ignorance of antidotes. Therefore conduct of a multicenter cohort study and experiments for ruling out chemicals according to chemicophysical priority as well as development of antidotes and clinical protocols for chemical injury patients is needed.