임상화학 검사의 Delta Check System 개발 및 정도관리 효율성 검토

권계철,임춘화,김문희,박연보 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Background : The application of the delta check in tests in general chemistry detects both random and clerical errors, thus enhancing the reliability of tests. However, since this approach adds the burden of confirming the results to the laboratory, it has the disadvantage of lengthening the turn around time of tests. We speculated that an online delta check system needed to reduce the turn around time. We developed delta quality control system and evaluated its effectiveness of quality control in clinical chemistry. Methods : We developed an online delta check system based on the client-server paradigm. We used IBM PentiumⅢ PC as server and as clients. The database system used was powerbuilder 6.0. Results : In the system we developed, delta screening was performed when test results were input to the computer. The decision over the delta screening was made by comparing it against patient's clinical information and cumulative results within the same screen. Conclusions : The developed delta check method made it available to reduce the turn around time previously spent on delta screening by eliminating the batch processing of tests which was needed in previous approaches separate query cumulative results and patient's clinical informations for screening purposes.

식품생물 산업기술의 새로운 미래를 선도하는 나노기술

김종태,김철진,조용진,전보연 한국산업식품공학회 2004 산업 식품공학 Vol.8 No.1

Nanotechnology represents a new frontier in science and technology with long term goal and benefits. Nowdays, nanotechnology is producing many revolutions and applications such as quantum computing, surface and materials modification, novel swparation and sensing technologies, and human biomedical replacements. These new techniques are being introduced in food and cio filed. In this review, the status and trends related to nanocomposites, nanoparticles, nanoyubes, delivery vehicles and biosafely were reviwed. The situation of funding of nanotechnology research in USA, Japan and Korea is also summarized.

P068 : The sebum secretion characteristics in trunk and its association with facial sebum secretion

( Bo Ri Kim ),( Mi Young Chun ),( Sung Ae Kim ),( Sang Woong Youn ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Background: To the best of our knowledge, there were no studies performed on the objective analysis of truncal sebum secretion. Objectives: The goal of this study was to investigate the characteristics of sebum secretion in face and trunk according to the each zone. We also evaluated the correlation between sebum secretion of face and trunk. Methods: The casual sebum levels (CSLs) of the five facial areas and ten trunk areas in 35 Korean females with acne were measured, using Sebumeter®. The areas of face and trunk were reclassified into low zone (U, FL, BL) and high zone (T, FH, BH) by CSLs. Statistical analysis was performed, according to the each zone. Results: The face showed higher CSLs than the trunk. The T zone showed higher CSLs than that of the U zone in the face. The FH zone had higher CSLs than that of the FL zone, and the BH zone had higher CSLs than that of the BL zone in the trunk. CSLs showed a significant positive correlation between T zone and FH zone. In addition, there was a significant positive correlation in the CSLs of FH zone and BH zone.Conclusion: This was the first study to report the characteristics of sebum secretion in trunk and the significant correlations between the CSLs of face and trunk using an objective, bioengineering method. We suggest that face and trunk can be divided into low zone and high zone according to the CSLs, and the patients who have higher CSLs in face are more likely to have higher CSLs in trunk.

Differential Interaction between Selenoprotein M and Apoptosis-Related Proteins in HEK 293 Cell and Transgenic Rat Overexpressing the Human Selenoprotein M

Dae Youn Hwang,Ji Soon Sin,Yong Kyu Kim,Chuel Kyu Kim,Byung Guk Kim,Sun Bo Shim,Seung Wan Jee,Su Hae Lee,Chang Jun Bae,Byoung Chun Lee,Mee Kyung Jang,Min Sun Kim,Su Youn Yim,Jung Sik Cho,Kab Ryong Cha 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.3

Selenoprotein M (SeIM) is a eukaryotic selenoprotein containing one selenocysteine (Sec) and Sec insertion sequence (SECIS) in 3' untranslated regions. In order to examine whether SelM expression and selenium treatment were influenced on expression level of the apoptosis-related proteins, pCMV/hlDE fusion gene were overexpressed in the HEK 293 cell line and hSelM-Tg rat. Herein, it is concluded that; ⅰ) two transgenic mice were identified by PCR analysis using specific primers by screening 28 newborn founder mice, ⅱ) the GFP-hSeIM fusion protein were successfully detected on the protein level in the HEK 293 cell line and hSeIM-Tg rat. ⅲ) it was also observed that p53 proteins were significantly changed in the HEK 293 cell under the condition of hSeIM overexpression and selenuim treatment, while not changed in the kidney tissue of hSeIM-Tg rat. ⅳ) Bax proteins were significantly changed in the kidney of hSeIM-Tg rat under the condition of hSeIM overexpression and sel treatment, while not changed in the HEK 293 cell line. ⅴ) Bel2 proteins in the HEK 293 cell were significantly decreased in the hSeIM transfectants, but hSeIM-Tg rat were shown the different expression patterns in the sel treated condition. These results suggest that selenium treatment and SeIM overexpression were differently influenced the expressions of apoptosis-related proteins in the tumor cell line and transgenic tissue

Plant Growth and Photosynthetic Characteristics of Yellow Flag (Iris Iris pseudacorus L.) to Hevymental Cadmium Treatment

Sung Chun Lee,Bo Young Jung,Seung Youn Lee,Gui Soon Kim,Jin Hee Gu,Jeong Sik Lee 한국생물환경조절학회 2007 한국생물환경조절학회 학술발표논문집 Vol.16 No.2

Tea Tree Essential Oil이 피부상태에 미치는 영향에 관한 연구 -청소년피부를 중심으로-

김보람 ( Bo Ram Kim ),윤천성 ( Chun Sung Youn ) 한국미용학회 2008 한국미용학회지 Vol.14 No.3

With the standard of living being elevated, people have an increasing interest in health and beauty. In particular, natural and eco-friendly beauty products, rather than products made of artificial chemicals, are gaining popularity as ways to enhance people`s skin conditions. In this regard, this paper studies the effect of Tea Tree essential oil, one of the natural products in the limelight, and also considers the oil`s potential to be used as natural antibiotics. The subject of this study is a group of 58 male and female middle school students in Daejeon and the group is divided into two groups of 29. The sample group is given cosmetics containing Tee Tree essential oil and the control group is given cosmetics without it. All of them are required to apply the cosmetics twice a day, once in the morning and once in the evening, for 4 weeks. The skin conditions of the two groups are measured by cornemeter, sebumeter and skin pH meter three times from the beginning of the test at two-week intervals over the four weeks` test period for comparison. The followings are the results that this study presents. The first hypothesis that “Tea Tree essential oil will have a positive effect on the level of moisture within the skin.” was denied. Skin condition is highly influenced by seasonal change. And as the study was conducted during the seasonal transition from late summer to autumn, reduction in skin`s moisture was detected in all subjects regardless of which group they belong to. The dry weather also caused dry skin making it difficult for us to precisely grasp how effective Tea Tree essential oil was on the subjects` skin. The second hypothesis that “Tea Tree essential oil will have a positive effect on the level of oil within the skin.” was supported. Tea Tree essential oil was found to balance the level of oil within skin by helping reduce oil in an excessively oily skin and by providing and maintaining a certain degree of oil in an oil-deprived dry skin. In short, Tea Tree essential oil has proved to have a certain function as a sebum controller. The third hypothesis that “Tea Tree essential oil will have a positive effect on the changes in skin pH.” was denied. Since not only the sample group but also the control group showed some changes in their skin pH levels, there was no evidence that Tea Tree essential oil had a direct impact on the pH level changes. Since it proves to improve the oil balance within people`s skin, Tea Tree essential oil is considered to strengthen skin`s resistance to external factors, sterilizing power and elasticity while preventing dry skin. For adults, Tea Tree essential oil is thought to help keep youthful skin by forming a protective film over it. And given the positive effects it has on the conditions of skin surface, there seems to be high possibility for Tea Tree essential oil to be used for the development of natural antibiotics and various medical cosmetics.

Bacterial Composition of Respiratory Organs and Intestine in Mice and Rats

Byoung Chun Lee,Mee Kyung Jang,Jong-Kun Seo,Jin-Ho Kang,Kab Ryong Chae,Dae Youn Hwang,Byoung Guk Kim,Seung Wan Jee,Sun Bo Shim,Su Hae Lee,Ji Soon Sin,Chang Jun Bae,Jong-Min Woo,Chuel Kyu Kim 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.1

The change of microbial flora in intestine and respiratory organs make some effects on animal health, which can cause some aberrations to experiment data. Therefore, it is an important factor to maintain the homogeneity of the bacterial composition of each organ. In this study, the bacterial distribution of respiratory organs and intestine were analyzed from 247 mice and 200 rats by analyzing biochemical properties and 16S rDNA sequencing. The total of 1,075 isolates were classified to 35 species of 17 genera. In case of rats, intestinal microbial flora were composed of 5 major bacteria including E. coli, P. mirabilis, M. morganii, C. freundii, and P. vulgaris, and 5 bacteria such as E. coli, P. mirabilis, S. aureus, S. cohnii, and M. morganii also mainly consisted of the microbial flora of respiratory organs. In case of mice, intestinal microbial flora were composed of 4 major bacteria including E. coli, P. mirabilis, A. lwoffi, and L. adecarboxylata, and 5 bacteria such as E. coli, B. sphaericus, S. auricularis, A. lwoffi, and L. adecarboxylata also mainly consisted of microbial flora of respiratory organs. This study suggested that B. sphaericus, G. morbillorum, S. paucimobilis, Staphylococcus spp. were specific in respiratory organs, and that M. morganii and Citrobacter spp. were almost specific in intestine. This study also can give some basic information to understand the relationship between animal care environment and microbial composition of animal organs.

산업용 연축전지의 충전특성실험에 근거한 축전효율 개선 알고리즘

朴允鎬(Youn-Ho Park),全舜鏞(Soon-Yong Chun),徐輔?(Bo-Hyeok Seo) 대한전기학회 2000 전기학회논문지 D Vol.49 No.8

Survey on the Current Status of Laboratory Animal Quality Control Program in Korea

Byoung Chun Lee,Mee Kyung Jang,Kab Ryong Chae,Dae Youn Hwang,Byoung Guk Kim,Seung Wan Jee,Sun Bo Shim,Su Hae Lee,Ji Soon Sin,Chang Jun Bae,Jong-Min Woo,Jung Sik Cho,Kwang Soo Joo,Chuel Kyu Kim 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.1

It is critical to harmonize the laboratory animal quality control program of each organization in order to improve the quality of laboratory animals. The aim of this study was to survey the current status of the laboratory animal quality control of 6 major animal suppliers and 14 Good Laboratory Practice (GLP) organizations. First of all, 6 suppliers provided more than 3 million animals, and 99% of them were specific pathogen-free (SPF) mice and rats. 88% of the GLP organizations were also using SPF animals, 81% of which were mice and rats. Two suppliers importing and offering animals were depending on the animal health report certified by providers, 2 suppliers were outsourcing to a domestic monitoring center, and 2 suppliers were monitoring in-house and simultaneously sending live animals to international centers for microbiological monitoring. All suppliers were monitoring the genetic integrity of inbred animals only once in a year. Fifty seven percentage of GLP organizations were doing the in-house microbiological monitoring, and only one of them was evaluating the genetic background of animals. Four suppliers monitoring animal quality were applying more than 5 samples of each animal facility unit quarterly. Fifty five percentage of GLP organizations monitored with each facility unit and 36% of them chose samples in the experimental group. Additionally, the animal number used in monitoring was 3-5, and monitoring frequency was 4 times in a year. Two suppliers sending animals to foreign companies for monitoring adopted almost all agents as testing items. Two suppliers were testing critical items including Ectromelia virus, Sendai virus, Mouse Hepatitis virus, Hantaan virus, Rat Coronavirus, Minute virus of mice, M. pulmonis, C. piliforme, and H. hepaticus. It were only 5 microoragnisms, MHV, Sendai virus, M. pulmonis, C. piliforme, and Ectoparasites which more than half of GLP organizations were monitoring commonly. Almost all organizations had their own SOPs for animal health monitoring and required a nationally harmonized guideline on the laboratory animal quality control. The result of this survey will be basic information on the national policy of the management of laboratory animal quality, and contribute to the improvement of laboratory animal quality in Korea.

Significant Change of Stress-related Proteins and Gene Profile in the Liver of Long-term Ground Transported Animals

Sun Bo Shim,Se Hyun Lee,Dae Youn Hwang,Chuel Kyu Kim,Bung Guk Kim,Yong Kyu Kim,Seung Wan Jee,Su Hae Lee,Ji Soon Sin,Chang Jun Bae,Byoung Chun Lee,Mi Kyong Jang,Min Sun Kim,Su Youn Yim,Jung Sik Cho,Kab 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.1

The transportation stress cause rise in the levels of many hormones and proteins to maintain homeostasis in the laboratory animals. In order to study whether the stress induced by long-term ground transport could contribute to change in the stress-related protein and profile of stress-regulated gene expression, these factors were detected in the animals between before and after the ground transportation. Herein, it is concluded that; ⅰ) the transported mice significantly exhibited the increase of corticosterone concentration in serum. ⅱ) the heat shock protein 70 (HSP70) in the liver were significantly decreased in the transported animals. ⅲ) 372 and 272 genes were up- and down-regulated, respectively, by stress in the long-term ground transported mice compare to the controls. ⅳ) genes encoding for immunity, signal transduction response and response to protein metabolism were significantly over-represented in the up-regulated group. ⅴ) among the down-regulated transcripts, those encoding for protein metabolism were dramatically over-represented, followed by those related to lipid metabolism and development process. These results suggested that the stress induced by ground transportation could significantly changed the level of stress-indicator hormone, proteins and gene profile in the mice. Therefor, the animal transported a long distance should be had the adaptation period to recover the normal physical state.