Variation in Phenotypic Traits in Onion (Allium cepa L.) Germplasm Collections

Binod Prasad Luitel(Binod Prasad Luitel ),한지원(JiWon Han),조명철(Myeong Cheoul Cho),최민선(Min-Seon Choi) 한국자원식물학회 2023 한국자원식물학회지 Vol.36 No.3

Dynamics of Microalgae Along the Coastal Areas of Sooyoung Bay, Busan, South Korea

Prasad, Binod,Thiyam, General,Lee, Dong-Gyu,Kim, Moo-Sang,Cho, Man-Gi The Korean Society for Marine Biotechnology 2011 한국해양바이오학회지 Vol.5 No.4

Microalgae are one of the major, sustaining components of ecosystem processes and are responsible for biogeochemical reactions that drive our climate changes. Despite this, many marine microalgae are poorly described and little is known of their abundance and distribution along the coastal areas of Sooyoung Bay, Busan, South Korea. The present study has been conducted from November, 2011 to August, 2009 with the objective to provide an overview of the taxonomy diversity and abundance of microalgae along the coastal areas of the Sooyoung Bay. Water samples were collected from different sites, which were located by using a GPS tracker. Chlorophyll fluorescence of the water samples were measured by using ToxY-PAM dual-channel yield analyzer. The chlorophyll fluorescence values were relatively higher during the spring and summer and even in the region near to the sea port. Similarly the abundance of microalgae was higher near the port but diversity index had lower values. The temperature and pH values were same at all the sites. However, only the temperature varied during the sampling period, with higher values during summer and lower in winter. From the preliminary results, the following class of microalgae were found; Bacillariophyceae, Dinophyceae, Silicoflagellate and Cryptophyceae. With a future ongoing work, microalgae are being isolated to establish single cell culture and for identification using light microscopic observations, photography and molecular approaches.

Yield in Pepper (Capsicum annuum L.) Cultivars Established at Different Planting Bed Size and Growth Containers

Binod Prasad Luitel,Taek Jong Lee,Tserendendev Oyuntugs,Won Hee Kang 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.5

A study was undertaken to investigate the optimum planting bed size and growth containers using masato soil on fresh yield of pepper cultivars in the production space of greenhouse at Hwacheon from June to October, 2009. Results showed that cultivars significantly affected on plant height, total fruit number, marketable fruit number (%) plant and total yield per plant whereas bed size significantly affected the plant height, marketable fruit number (%), marketable and non-marketable weight (g) per plant. Planting transplants at 60㎝ bed width gave the highest total fruit number (61.8), marketable fruit number (58.3%) and weight (1,119.4 g) per plant. Growth containers significantly affected the total fruit number, marketable and non-marketable fruit number (%) and total fruit yield (g) per plant. ‘Chungyang’ and ‘Nokkwang’ grown on plastic pot gave higher total fruit number, marketable fruit number percentage and total yield per plant but ‘Special’, ‘Minipaprika’ and ‘Pimiento’ grown in Styrofoam trays significantly gave higher total fruit (no), marketable fruit number (%) and total yield (g) per plant. Hence, in masato soil, 60cm planting bed width in Styrofoam trays can be used to obtain better yield in peppers as well as to optimize the production space in plastic house.

Dynamics of Microalgae Along the Coastal Areas of Sooyoung Bay, Busan, South Korea

Binod Prasad,General Thiyam,Dong-Gyu Lee,Moo-Sang Kim,Man-Gi Cho 한국해양바이오학회 2011 한국해양바이오학회지 Vol.5 No.1

Genetic Variability and Traits Relationship Studies of WorldVeg Tomato Genotypes in Nepal

Binod Prasad Luitel,Dipendra Ghimire,Surendra Lal Shrestha,정효봉,양은영,조명철 한국국제농업개발학회 2023 韓國國際農業開發學會誌 Vol.35 No.3

This study was conducted to assess the genetic variability and correlation of phenotypic characteristics in 12 tomato (Solanum lycopersicum L.) genotypes including 11 WorldVeg and one commercial variety (Pusa Ruby) in Terai (plain) region of Nepal in 2021–2022. This experiment was laid out in a randomized complete block design with three replications. The phenotypic traits, including days to 50% flowering, plant vigor and height, fruit number/plant, fruit yield, fruit weight and diameter, fruit firmness and fruit pericarp thickness, and total soluble solids (TSS) content of the fruits, were studied. Analysis of variance revealed significant differences among the genotypes for all the traits except for plant vigor. The genotype of AVTO1705 resulted the highest fruit yield (2.9 kg/plant) than Pusa Ruby, a commercial check (0.5 kg/plant). The phenotypic coefficient of variation (PCV) was higher than the genotypic coefficient of variation (GCV) for all the traits and PCV values were maximum for the number of fruits, fruit yield, and fruit weight. High PCV, GCV, and genetic advance (GA) were observed for yield, fruit weight, and plant height, respectively, indicating the additive gene effect. High heritability for fruit yield/plant and plant height inferred the phenotypic selection for their genetic improvement. Fruit yield was significantly (P<0.05) positively correlated with the fruit number and fruit weight, and direct selection of these traits are reliable for yield improvement in tomato.

Yield and Fruit Quality of Tomato (Lycopersicon esculentum Mill.) Cultivars Established at Different Planting Bed Size and Growing Substrates

Binod Prasad Luitel,Prakash Babu Adhikari,Cheol Soo Yoon,Won Hee Kang 한국원예학회 2012 한국원예학회 학술발표요지 Vol.2012 No.5

Yield and Fruit Quality of Tomato (Lycopersicon esculentum Mill.) Cultivars Established at Different Planting Bed Size and Growing Substrates

Binod Prasad Luitel,Prakash Babu Adhikari,윤철수,강원희 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.2

This study was carried out to investigate the yield and fruit quality of tomato (Lycopersicon esculentum Mill.)cultivars using different plant bed width and growing substrates in Hwacheon Farm, 2011. Transplants of tomato cultivars ‘Campari’, ‘Temptation’, ‘Annamay’, and ‘Adoration’ were planted at 20 cm, 40 cm, 60 cm, and 80 cm bed width and same cultivars were grown at cocopeat, rockwool and masato in early July, 2011 in a greenhouse. Planting bed size significantly affected the fruit number, marketable fruit number (%) and weight (g), and total yield (g) per plant. Plants grown at 60 cm bed width yielded the highest fruit number (22.4), marketable fruit number (56.0%), marketable fruit weight (356.8 g) and total yield (564.5 g) per plant. Bed width had no significant effect on fruit size and quality characters. Plants grown in cocopeat produced the highest marketable fruit number (56.2%) per plant and yielded the greatest (445.6g) marketable yield per plant. Plants grown in cocopeat substrate produced higher fruit number (5.2%) and total yield (0.7%) than that of rockwool substrate. Fruit size and fruit quality characters showed no significant differences within growing substrates. Hence, planting the tomato cultivars in single row at 60 cm bed width is better approach to optimize the production space in greenhouse and cocopeat is recommended as potential growing substrates for tomato cultivation to increase its yield and fruit quality characters. This study was carried out to investigate the yield and fruit quality of tomato (Lycopersicon esculentum Mill.)cultivars using different plant bed width and growing substrates in Hwacheon Farm, 2011. Transplants of tomato cultivars ‘Campari’, ‘Temptation’, ‘Annamay’, and ‘Adoration’ were planted at 20 cm, 40 cm, 60 cm, and 80 cm bed width and same cultivars were grown at cocopeat, rockwool and masato in early July, 2011 in a greenhouse. Planting bed size significantly affected the fruit number, marketable fruit number (%) and weight (g), and total yield (g) per plant. Plants grown at 60 cm bed width yielded the highest fruit number (22.4), marketable fruit number (56.0%), marketable fruit weight (356.8 g) and total yield (564.5 g) per plant. Bed width had no significant effect on fruit size and quality characters. Plants grown in cocopeat produced the highest marketable fruit number (56.2%) per plant and yielded the greatest (445.6g) marketable yield per plant. Plants grown in cocopeat substrate produced higher fruit number (5.2%) and total yield (0.7%) than that of rockwool substrate. Fruit size and fruit quality characters showed no significant differences within growing substrates. Hence, planting the tomato cultivars in single row at 60 cm bed width is better approach to optimize the production space in greenhouse and cocopeat is recommended as potential growing substrates for tomato cultivation to increase its yield and fruit quality characters.

In Vitro Androgenic Response of Minipaprika (Capsicum annuum L.) Genotypes in Different Culture Media

Binod Prasad Luitel,강원희 한국원예학회 2013 Horticulture, Environment, and Biotechnology Vol.54 No.2

The in vitro androgenic response of minipaprika F1 hybrid cv. Vine sweet (red, yellow, and orange form)was investigated using Dumas de Vaulx (CP) and Murashige and Skoog (MS) culture media to ascertain the effectiveness of androgenesis in haploid production. The frequency of callus and embryo formation varied in anther cultures of all minipaprika forms in both culture media. Anthers in both culture media responded to form the callus without regeneration in all minipaprika forms. The anthers of ‘Vine sweet-yellow’ produced 62.5% and 46.7% normal embryo in CP and MS medium, respectively. ‘Vine sweet-yellow’ produced almost four fold more normal-looking embryos than ‘Vine sweet-red’ in both culture media, and ‘Vine-sweet orange’ showed low androgenic response to anther culture. The anthers cultured in CP medium gave more normal embryo in all minipaprika forms as compared to MS medium. Among a total of 51 embryos transferred to growth regulator free MS medium for regeneration, 45plants were regenerated. Flow cytometry analysis revealed that 44.5%, 42.4%, and 33.3% plants were haploids, and 55.5%, 57.6%, and 66.7% plants were spontaneous diploids in red, yellow and orange forms, respectively. The chromosome number of haploid plant was 12 and that of spontaneous diploid was 24. Stomata characters and chloroplast count in the guard cell of leaf stomata were also found to be reliable and simple method to analyze the ploidy level of regenerated plants. The spontaneous diploids confirmed as doubled haploids (DHs) following the self-pollination. The obtained DH and haploid plants from anther culture would be the valuable breeding materials for heterosis breeding.

Variation in Seed Yield and Yield-related Traits of Onion (Allium cepa L.) Germplasm Collections

Binod Prasad Luitel,JiWon Han,Min-Seon Choi 한국원예학회 2023 한국원예학회 학술발표요지 Vol.2023 No.5

네팔의 식물유전자원 보유현황과 국립종자은행의 자원연구 및 관리현황

Binod Prasad Luitel,Krishna Hari Ghimire,Bal Krishna Joshi,Kyoung-Yul Ryu,Sung Jung-Sook,Juhee Rhee,Sang-Gyu Kim,Ho-Cheol Ko,Hyung-Jin Baek,Moon-Sup Yoon,On-Sook Hur 한국국제농업개발학회 2016 韓國國際農業開發學會誌 Vol.28 No.2

The main objective of this paper is to elucidate the present status of Plant Genetic Resources (PGRs), their research, and management system in the genebank of Nepal. PGRs for food and agriculture are the biological basis of world food and nutrition security. The remarkable elevation ranging from 60 meter above sea level (masl) to highest summit Mount Everest (8,848 masl) has created huge geographical and climatic variation which harbor enormous plant species in the country. National Agriculture Genetic Resource Center (NAGRC) or ‘Genebank’ currently conserved 11,051 accessions of more than 110 crops species belonging to cereals (5,850), pseudo cereals (1,150), pulses (1,800), oilseeds (185), and vegetables (565) including other crops (1,501) at ex-situ. Diversity mapping, characterization and evaluation of PGRs, duplicates identification, diversity study, pre-breeding and landrace enhancement are the major research works of NAGRC, and its management strategies include conservation method, types, and groupings of PGRs. Characterization, evaluation and tagging of economically important traits in PGRs are now more important for strengthening their pre-breeding and proper utilization. Additionally, strong communication and collaborative network among public, private, community based organizations and international organizations are important for the effective management of PGRs.