Basic Fibroblast Growth Factor Increases Intracellular Magnesium Concentration through the Specific Signaling Pathways

Bing-Zhe Hong,박선아,Han-Na Kim,Tian-Ze Ma,Han-Gyu Kim,강형섭,김환규,곽용근 한국분자세포생물학회 2009 Molecules and cells Vol.28 No.1

Basic fibroblast growth factor (bFGF) plays an important role in angiogenesis. However, the underlying mechanisms are not clear. Mg2+ is the most abundant intracellular divalent cation in the body and plays critical roles in many cell functions. We investigated the effect of bFGF on the intracellular Mg2+ concentration ([Mg2+]i) in human umbilical vein endothelial cells (HUVECs). bFGF increased [Mg2+]i in a dose-dependent manner, independent of extracellular Mg2+. This bFGF-induced [Mg2+]i increase was blocked by tyrosine kinase inhibitors (tyrphostin A-23 and genistein), phosphatidylinositol 3-kinase (PI3K) inhibitors (wortmannin and LY294002) and a phospholipase Cγ (PLCγ) inhibitor (U73122). In contrast, mitogen-activated protein kinase inhibitors (SB202190 and PD98059) did not affect the bFGF-induced [Mg2+]i increase. These results suggest that bFGF increases the [Mg2+]i from the intracellular Mg2+ stores through the tyrosine kinase/PI3K/PLCγ- dependent signaling pathways.

H2O2가 hKv1.5 전류에 미치는 영향

홍병철 ( Bing Zhe Hong ),유춘원 ( Chun Won Yoo ),김한규 ( Han Kyu Kim ) 전북대학교 의과학연구소 2004 全北醫大論文集 Vol.28 No.1

Hydrogen peroxide의 농도에 따른 심장기능 조절작용을 규명하고자, 심방에서 중요한 기능을 한다고 알려진 hKv1.5유전자를 HEK-293 cell 및 Ltk- cell에 발현시켜 유도된 K* 전류 및 사람 심방 세포의 K*전류에 미치는hydrogen peroxide의 영향을 관찰하여 다음과 같은 결과를 얻었다. 1. Hydrogen peroxide는 HEK-293 cell 및 Ltk- cell에서 Kv1.5 전류의 activation curve의 전압의존성을 과분극 쪽으로 약20 mV정도 이동시켜 전류를 증가시켰다. 또한, HEK-293 cell에서 Kv1.5/Kvβ1.3전류의 activation curve의 전압의존성올 이동시켰을 뿐 만 아니라 fast inactivation을 하였다. 2. Ltk- cell에서 0.01-0.3 mM의 hydrogen peroxide는 Kv1.5를 증가시켰고 1 mM이 상에서는 현저히 감소시켰다. 3. 사람 심방세포서 1 mM의 hydrogen peroxide는 steady state outward 전류를 5배 이상 증가시켰다. 이상의 실험성적은 hydrogen peroxide가 농도에 따라 hKv1.5전류를 조절할 수 있음을 밝혔는데, 특히 비교적 낮은 농도의 hydrogen peroxide는 이 전류를 증가시킴을 사람심방세 포에서 확인하였다. During myocardial ischemia and subsequent reperfusion, reactive oxygen species are an important mediator of cellular damage and rhythm disturbances. H2O2, a small, uncharged and relatively stable molecule that diffuses easily through tissue, has several adverse effects on the myocardium including induction of cardiac arrhythmias. Although several previous investigations have described electro-physiological effects of H2O2 in mammalian myocardium, the underlying ionic mechanisms of H2O2 effects remain unclear. In addition, voltage gated K* channels represent the most complex group of ion channel genes expressed in cardiovascular system. The human Kv1.5 channel (hKv1.5) represents the IKur repolarizing current in atrial myocytes. The hKv1.5 channel is functionally modulated by the Kvβ1.3 subunit, which converts it from a delayed rectifier to a channel with rapid inactivation and enhanced voltage sensitivity. In present study, we examined the effects of H2O2 following coexpression of hKv1.5 with the Kvβ1.3 subunit in LtK-cells and HEK-293 cells. H2O2 (1 mM) reversibly increased the K* currents in the cells transected with hKv1.5 and/or Kvβ1.3 or Kvβ2.1 subunits. Additionally, H2O2 slowed deactivation and fast inactivation in the cells cotransfected with hKv1.5 and/or Kvβ1.3 or Kvβ2.1, and made the hyperpolizing shift of activation curve by about 20 mV. However, higher concentration of H2O2 (higher than 1 mM) decreased the hKv1.5/Kvβ2.1 current. Catalase (1000 u/ml) itself accelerated the inactivation process of hKv1.5 current and decreased the steady state current. In isolated human atrial myocytes, H2O2 (1 mM) reversibly increased the outward K* currents by about five times. These results reported that H2O2 has a dual effect on hKv1.5 current, thereby being able to explain the potential roles of H2O2 during myocardial reperfusion injury.

Association of Visit-to-Visit Variability of Blood Pressure with Cardiovascular Disease among Type 2 Diabetes Mellitus Patients: A Cohort Study

Zhe-Bin Yu,Die Li,Xue-Yu Chen,Pei-Wen Zheng,Hong-Bo Lin,Meng-Ling Tang,Ming-Juan Jin,Jian-Bing Wang,Kun Chen 대한당뇨병학회 2019 Diabetes and Metabolism Journal Vol.43 No.3

Background: Increasing evidence has shown that visit-to-visit variability (VVV) of blood pressure (BP) is associated with an increased risk of cardiovascular disease (CVD). The objective of this study was to evaluate the impact of VVV of systolic blood pressure (SBP) and diastolic blood pressure (DBP) on the risk of CVD among patients with type 2 diabetes mellitus (T2DM) in China. Methods: We conducted a retrospective cohort study of 10,163 T2DM patients who were not previously diagnosed with CVD from January 2008 to December 2012 in Ningbo, China. The VVV of BP was calculated using five metrics, including standard deviation (SD), coefficient of variation (CV), variation independent of mean, average real variability, and successive variability (SV) of measurements, obtained over a 24-month measurement period. Hazard ratios and 95% confidence intervals (CIs) were estimated by Cox proportional hazards regression models for the associations of variability in BP with risk of CVD. Results: A total of 894 CVD events were observed during a median follow-up of 49.5 months. The hazard ratio in the highest quintile of SD of SBP was 1.24 (95% CI, 1.01 to 1.52) compared with patients in the lowest quintile. The association between higher VVV of DBP and risk of CVD was not consistent across different metrics and sensitivity analyses. Conclusion: Higher VVV of SBP was associated with an increased risk of CVD, irrespective of the mean SBP level. Future studies are needed to confirm these findings.

Asian Type 감마 3 정을 이용한 대퇴골 전자간 골절의 치료결과 분석

황병철 ( Bing Zhe Huang ),박용욱 ( Yong Wook Park ),박진수 ( Jin Su Park ),노규철 ( Kyu Cheol Noh ),김성연 ( Soung Yon Kim ),정국진 ( Kook Jin Chung ),김홍균 ( Hong Kyun Kim ),김형년 ( Hyong Nyun Kim ),윤용현 ( Yong Hyun Yoon ) 대한골절학회 2014 대한골절학회지 Vol.27 No.3

Purpose: This study was conducted in order to demonstrate the radiologic and clinical results of using the Asian type gamma 3 nail in treatment of trochanteric fractures. Materials and Methods: Patients underwent operations with gamma 3 nails between August 2010 and August 2012. For the radiologic evaluation, we analyzed bone quality, fracture pattern, reduction quality, union period, and position of lag screw (tip-apex distance, Cleveland index). For the clinical evaluation, we analyzed mobility score of Palmer and Parker and Jensen`s functional score. Results: The mean union was taken at 17.4 weeks (14-25 weeks). Two cases (4.3%) underwent reoperation due to cutting out of the hip screws. The clinical outcomes of Parker and Palmer`s average mobility score changed from 7.3 to 5.8 (79%), Jensen`s functional score was 1.3 to 1.8 (72%). Conclusion: We achieved excellent clinical and radiological outcomes; therefore, Asian type gamma 3 nail is effective in treatment of trochanteric fractures of the femur in Korea.

A 2D/2D BiPO4/g-C3N4-B Z-type heterojunction for enhanced photocatalytic degradation of dye pollutants

Zhao Hong-jian,Zhou Yan,Wu Ren-Jang,Han Zheng-bing,Li Xu,Yu Zhe 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.12

A 2D/2D BiPO4/g-C3N4-B nano-sheet heterojunction photocatalyst was synthesized via a simple coprecipitation method at room temperature using glacial acetic acid as solvent, which showed excellent activity toward the degradation of rhodamine B (RhB). The heterojunction showed much higher efficiency of separation and transfer of photogenerated carriers compared to that of its constituents. Moreover, the spectral response range of BiPO4 was effectively broadened after the combination of g-C3N4-B and BiPO4. Consequently, a 97.3% degradation of RhB within 25 min by BiPO4/g-C3N4-B heterojunction photocatalyst under visible light irradiation was observed. The difference in work functions of BiPO4 and g-C3N4-B was evident from UPS characterization, which led to the bending of the energy band and the establishment of an internal electric field at the interface of the heterojunction. Therefore, the synthesized direct Z-type BiPO4/g-C3N4-B heterojunction enhanced the oxidation-reduction ability by promoting the effective separation of photogenerated carriers.

Role of Gap Junction in the Regulation of Renin Release and Intracellular Calcium in As 4.1 Cell Line

Jeong Hee Han,Bing-Zhe Hong,Young Geun Kwak,Kuichang Yuan,Woo Hyun Park,Sung Zoo Kim,Suhn Hee Kim 대한생리학회-대한약리학회 2007 The Korean Journal of Physiology & Pharmacology Vol.11 No.3

Gap junction protein, connexin, is expressed in endothelial cells of vessels, glomerulus, and renin secreting cells of the kidney. The purpose of this study was to investigate the role of gap junction in renin secretion and its underlying mechanisms using As 4.1 cell line, a renin-expressing clonal cell line. Renin release was increased proportionately to incubation time. The specific gap junction inhibitor, 18-beta glycyrrhetinic acid (GA) increased renin release in dose-dependent and time- dependent manners. Heptanol and octanol, gap junction blockers, also increased renin release, which were less potent than GA. GA-stimulated renin release was attenuated by pretreatment of the cells with amiloride, nifedipine, ryanodine, and thapsigargin. GA dose-dependently increased intracellular Ca<SUP>2+</SUP> concentration, which was attenuated by nifedipine, nimodipine, ryanodine, and thapsigargin. However, RP-cAMP, chelerythrine, tyrphostin A23, or phenylarsine oxide did not induced any signi</SUP>ficant change in GA-stimulated increase of intracellular Ca2+ concentration. These results suggest that gap junction plays an important role on the regulation of renin release and intracellular Ca<SUP>2+</SUP> concentration in As 4.1 cells.

Role of Gap Junction in the Regulation of Renin Release and Intracellular Calcium in As 4.1 Cell Line

Han, Jeong-Hee,Hong, Bing-Zhe,Kwak, Young-Geun,Yuan, Kui-Chang,Park, Woo-Hyun,Kim, Sung-Zoo,Kim, Suhn-Hee The Korean Society of Pharmacology 2007 The Korean Journal of Physiology & Pharmacology Vol.11 No.3

Gap junction protein, connexin, is expressed in endothelial cells of vessels, glomerulus, and renin secreting cells of the kidney. The purpose of this study was to investigate the role of gap junction in renin secretion and its underlying mechanisms using As 4.1 cell line, a renin-expressing clonal cell line. Renin release was increased proportionately to incubation time. The specific gap junction inhibitor, 18-beta glycyrrhetinic acid (GA) increased renin release in dose-dependent and time-dependent manners. Heptanol and octanol, gap junction blockers, also increased renin release, which were less potent than GA. GA-stimulated renin release was attenuated by pretreatment of the cells with amiloride, nifedipine, ryanodine, and thapsigargin. GA dose-dependently increased intracellular $Ca^{2+}$ concentration, which was attenuated by nifedipine, nimodipine, ryanodine, and thapsigargin. However, RP-cAMP, chelerythrine, tyrphostin A23, or phenylarsine oxide did not induced any significant change in GA-stimulated increase of intracellular $Ca^{2+}$ concentration. These results suggest that gap junction plays an important role on the regulation of renin release and intracellular $Ca^{2+}$ concentration in As 4.1 cells.

V. vulnificus의 cyolysin에 의해 형성된 Pored의 이온 투과성에 관한 연구

마천택 ( Tian Ze Ma ),홍병철 ( Bing Zhe Hong ),고경희 ( Hee Ko Kyeung ) 전북대학교 의과학연구소 2004 全北醫大論文集 Vol.28 No.1

V. vulnificus가 분비하는 cytolysin의 pore 형성 효과를 전기생리학적으로 확인하고 그 pore의 이온선택성을 밝히고자, V. vulnificus cytolysin의 표적세포로 예산되는 폐내피세포인 CPAE 세포에서 여러 bath용액에서의 cytolysin pore의 이온투과성을 비교 관찰하여 다음과 같은 결과를 얻었다. 1. Cytolysin 1 HU/ml을 세포내외에 투여시 2-7분의 lag time 후 sensitivity 차이없이 115.6 ± 5.1 pS의 단일 전도도를 갖는 pore를 시간 의존적으로 형성하였으며, 이런 pore의 이온투과성은 세척 후에도 지속적으로 유지되었다. 2. Bath용액의 140 mM KCI을 같은 농도의 NaC1 및 CsC1로 치환하여도 cytolysin pore 의 이온투과성에는 유의한 변동이 없었다. 3. Bath용액의 140 mM KCI을 같은 농도의 CaC1(2) 및 BaCl2로 치환하면 cytolysin pore의 이온투과성이 각각 1.8배와 2.3배로 현저하게 증가하였다. 4. Bath용액의 C1 농도를 동일하게 하고자, 각각 140 mM KCI과 70 mM MgC1(2)로 치환시 cytolysin pore의 이온투과성에는 유의한 차이가 없었다. 5. Bath용액의 KCI농도를 140 mM에서 10mM과 1 Mm로 감소시 cytolysin pore의 역전전압이 0 mV에서 각각 -31.1 ± 1.5mV와 -42.3 ± 1.8 mV로, C1 이온의 역전전압에 근접하게 이동하였다. 이상의 실험결과를 종합하변, V. vulnificus cytolysin은 세포막내외의 차이없이 시간의존 적인 pore를 형성하는데, 이 pore의 투과성은 막전위에 비의존적이며 주로 C1 이온에 선택성이 높음을 시사한다. Cytolysin produced by V. vulnificus has been incriminated as one of the important virulence determinants in V. vulnificus infection. Ion selectivity of cytolysin-induced pres has been examined in CPAE cells, a cell line of pulmonary endothelial cells, using inside-out patch clamp techniques. In symmetrical KCI concentration (140 mM). intracellular and extracellular cytolysin formed the ion-permeable pores with single channel conductance of 115.6±5.1 pS (n=6) in a time-dependent manner and a voltage-independent manner. The pore currents were consistently maintained after washout of cytolysin. Replacement of intracellular 140 mM KCI with 140 mM NaCl, 140 mM CsCl and 70 mM MgCl2 did not affect the pore currents, but replacement of it with equimolar CaCl2 and BaCl2 increased the pore current by 1.8 and 2.4 times of control current (140 mM KCL), respectively. When the intracellular KCI concentration was lowered from 140 to 10 and 1 mM, zero-current membrane potentials shifted from 0 mV to -31.1 ± 1.5 and -42.3 ± 1.8 mV, respectively. These results indicate that cytolysin produced by V. vulnificus formed the CI(-) -selective pores in CPAE cells.

Decreased Complete Oxidation Capacity of Fatty Acid in the Liver of Ketotic Cowsa

Xu, Chuang,Liu, Guo-wen,Li, Xiao-bing,Xia, Cheng,Zhang, Hong-you,Wang, Zhe Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.3

Complete oxidation of fatty acid in the liver of ketotic cows was investigated. Serum non-esterified fatty acid (NEFA), beta-hydroxybutyric acid (BHBA) and glucose concentrations were measured using biochemical techniques. Carnitine palmitoyl transferase II (CPT II), 3-hydroxy acyl-CoA dehydrogenase (HAD) and oxaloacetic acid (OAA) concentrations in the liver were detected by ELISA. Serum glucose was lower in ketotic cows than controls (p<0.05). Serum BHBA and NEFA concentrations were higher in ketotic cows than controls (p<0.05). OAA, CPT II, and HAD contents in the liver of ketotic cows were lower than in controls (p<0.05). There were negative correlations between serum NEFA concentration and OAA, CPT II and HAD, but no correlation between serum BHBA concentration and capacity for complete oxidation of fatty acid. Overall, the capacity for complete fatty acid oxidation in the liver of ketotic cows might have been decreased. High serum NEFA concentrations may be unfavorable factors for the pathway of complete oxidation of fatty acid in the liver.