대장암 세포암종 HCT - 15 세포 및 위암 세포암종 AGS 세포에서 차가버섯 조성물에 의한 세포생육 억제 효과

차재영(Jae-Young Cha),전병삼(Beong-Sam Jeon),문재철(Jae-Chul Moon),유지현(Ji-Hyun Yoo),조영수(Young-Su Cho) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.4

녹차분말을 포함하는 차가버섯 조성물의 수용성 추출물에 의한 인체 위암 세포 AGS 및 대장암 세포 HCT-15, 그리고 마우스 정상세포 NIH3T3 fibroblast의 세포생육에 미치는 효과를 세포수 측정방법과 MTT assay 방법으로 측정하였다. 차가버섯 조성물의 수용성 추출물은 인체 대장암 세포주 HCT-15와 위암 세포주 AGS의 생육을 억제하였다. 그러나 동일한 실험조건하에서 마우스 정상 세포주 NIH3T3은 80% 이상의 생존율을 나타내었다. 본 연구의 결과로 차가버섯 조성물의 수용성 추출물은 정상세포에는 독성을 나타내지 않으면서 위암 및 대장암 세포에는 높은 생육억제 효과를 나타냄을 알 수 있었다. This study was performed to investigate the cytotoxic effect of the water-extract from Chaga mushroom (Inonotus obliquus) compositions containing powdered green tea in HCT-15 human colon carcinoma, AGS human gastric carcinoma and NIH3T3 mouse normal fibroblast cells using viable cell count and MTT assay. The water-extract from Chaga mushroom compositions induced inhibitory effects on proliferation of HCT-15 and AGS cells in the MTT assay and viable cell count. However, mouse normal NIH3T3 cells were exhibited 80% survival under the same condition. Chaga mushroom compositions showed highly antiproliferative effect in human cancer cell line HCT-15 and AGS, but not in mouse normal cell line NIH3T3. These results suggest that Chaga mushroom (Inonotus obliquus) compositions containing powdered green tea are the candidate for chemoprevention in colon and gastric cancer.

식품 : 차가버섯과 어성초 함유 발효 조성물이 인체 위암 AGS 및 대장암 HCT-15 세포 생육에 미치는 영향

차재영 ( Jae Young Cha ),전병삼 ( Beong Sam Jeon ),박정원 ( Jeong Won Park ),문재철 ( Jae Chul Moon ),조영수 ( Young Su Cho ) 한국응용생명화학회(구 한국농화학회) 2004 Applied Biological Chemistry (Appl Biol Chem) Vol.47 No.2

사람 양수중 다종의 세포외성 포스포리파제 A₂의 부분정제 및 특성

전용주,백석환,이지혜,문태철,민병우,장현욱 영남대학교 약품개발연구소 1997 영남대학교 약품개발연구소 연구업적집 Vol.7 No.-

Multiple forms of extracellular phospholipase A₂ have been detected in human amniotic fluid (HAF). When HAF was subjected to heparin-Sepharose column chromatography, phospholipase A₂activity was deleced in both heparin-non binding and binding fraction. The activity of heparin-non binding fraction was further purified by sequential uses of column chromatographies on butyl-Toy-opearl 659M and DEAE-Sephacel. DEAE-SEphacel fraction contained three different phospholipase A₂ activities (Peak Ⅰ, Ⅱ, Ⅲ). The molecular weight of DEAE-Sephacel fraction phospholipase A₂ determined by SDS-PAGE were about 52KDa (Peak Ⅰ). Peak Ⅱ, Ⅲ required micromolar Ca^(2+) ion for its maximun activity, but Peak Ⅰ enzyme showed calcium independent phospholipase A₂ activity and showed broad range of pH (6.0~10.0) optimum. All these enzymes were not recognized by a monoclonal antibody reised against phospholipase A₂ from human synovial fluid. These results suggest that HAF might contain multiple forms of extracellular phospholipase A₂ which may neither belong to the 14KDa group Ⅱ phospholipase A₂ family nor cytosolic phospholipase A₂.

혹명나방저항성벼의 잡초화 및 유전자 이동 가능성

이인용(In-Yong Lee),박재읍(Jae-Eup Park),문병철(Beong-Chul Moon),서석철(Seok-Cheol Suh),신공식(Kong-Sik Shin),우미옥(Mi-Ok Woo),권순종(Soon-Jong Kweon) 韓國雜草學會 2009 Weed&Turfgrass Science Vol.29 No.1

혹명나방저항성벼(06’ : Event C7-1-9-1, ‘07년 Event C7-1-9-1-1, ’08년 Event C7-1-9-1-1-1)의 잡초화 및 유전자 이동 가능성을 조사하기 위하여 여러 가지 시험을 수행하였다. 토양 중에 매몰한 후 1개월 경과된 모든 종자는 100% 발아하였으나, 3개월 이후에는 모두 발아율이 0%이었다. 수확과정에서 탈립된 종자 중 자연노출조건에서 추청벼는 27% 발아한 반면에 GM벼는 2.7%, 낙동벼는 3.3% 발아 되어 월동조건에 따라 발아정도가 상이하였으나, 물에 떨어진 종자는 익년도에 발아가 되지 않았다. 비의도적으로 일출된 혹명나방저항성벼는 글리포세이트액제나 패라쾃디클로라이드액제와 같은 비선택성 제초제로 방제 가능하였다. 혹명나방저항성벼와 재배벼인 낙동벼 간의 유전자 이동여부는 거리별로 수확한 후 바스타액제 처리 결과, 근접거리가 가까운 벼에서 바스타에 생존하는 비율이 높아 화분교잡에 의한 유전자 이동 비율이 8.6~11.6%를 보였다 이러한 유전자 이동여부는 test kit를 이용하여 유전자 함유여부를 최종 확인할 수 있었다. 저항성벼와 잡초성벼간의 유전자 이동시험에서 바스타 처리시 생존하는 개체가 없었는데 이러한 결과는 출수기가 달랐기 때문으로 사료된다. 시험포장에 발생된 잡초는 일년생 및 다년생잡초가 고르게 분포되었으며, 혹명나방저항성벼와 인근 포장이 잡초발생 양상이 유사하였다. This study was carred out to investigate the possibility of weediness and gene flow from leaf-folder(Cnaphalocrocis medinalis) resistant rice (06’ : Event C7-1-9-1. ‘07 : C7-1-9-1-1, ’08 : C7-1-9-1-1-1). The leaf-folder resistant rice was germinated 100% at 1 month after bury into soil and was showed 0% after 3 month. The shattered rice seeds during harvest were germinated 27% in Chunchungbyeo, 2.7% in GM rice and 3.3% in Nakdongbyeo under the field condition. However, germination pattern was different according to overwinter condition. When GM rice was unintentional escape in outdoor of the leaf-folder resistant rice can be controlled with glyphosate and paraquat of non-selective herbicides. In order to investigate gene flow from the leaf-folder resistant rice, Nakdongbyeo and weedy rice were planted with difference distance. Degree of survival was higher at near planting Nakdongbyeo from the leaf-folder resistant rice. The detection of gene flow from the leaf-folder resistant rice was done using a lateral flow strip test kit. Gene of the leaf-folder resistant rice can be detected by lateral flow strip test. But showed no survival plant from weedy rice because of differential stage of heading. Weed flora in the culture field of leaf-folder resistant rice were very similar with culture field of non GM rice.

우리나라 잡초이름으로 알아본 언어학적 고찰

이인용(In-Yong Lee),김창석(Chang-Seog Kim),문병철(Beong-Chul Moon),오세문(Se-Mun Oh),박재읍(Jae-Eup Park) 韓國雜草學會 2009 Weed&Turfgrass Science Vol.29 No.3

한국잡초학회에서 발간한 잡초학용어집에 수록된 638종을 대상으로 하여 크기, 형태, 계절, 동물 및 곤충, 참과 거짓, 지역, 색상, 사람, 유사성, 오감 등으로 나누고 이를 언어학적으로 고찰하였다. 크기를 나타내는 접두어로 가는/쇠/실(narrow, small, 細), 애기(small, 小), 좀/좁쌀(little, bit, 小), 큰(big, 大), 긴(long, 長)등이 있으며, 형태(모양)를 나타내는 접두어로는 가시/바늘(thorn, needle, 針), 누운(lie, 臥), 등근(round, circular, 圓), 선(stand, 立), 주름(creases, folds, wrinkled), 털(hair, 毛)등이 있다. 또한, 개, 고양이, 소, 쥐 등과 같은 우리 주변에 있는 동물 또는 곤충을 의인화한 잡초가 있다. 진짜를 의미하는 참(real, 眞)이 들어간 잡초로는 참방동사니, 참소리쟁이 등이 있으며, 거짓 또는 질이 떨어지는 것을 뜻하는 개(plain, 野), 물가나 물(water, 水)과 관련되어 물이라는 접두어가 붙은 잡초가 있다. 문제 외래잡초에는 미국(美國), 서양/양(Western, 西洋), 구주/유럽(Europe, 歐洲)이라는 접두어를 붙였다. 잡초의 외관이나 꽃이 특유의 색상이 잡초명에 포함된 경우도 있는데 노란색(yellow, 黃), 붉은색(red, 赤), 자주/주홍(purple, violet, 紫), 흰색(white, 白)등이 있다. 잡초 중에는 사람을 의인화하거나 비속화시켜 이름을 붙인 경우도 있는데 광대(clown, 廣大), 기생(妓生), 난쟁이(dwarf, 侏儒), 바보(fool) 등이 있다. 이밖에 유사성을 강조하여 나도(me too) 또는 너도(you too)라는 단어가 붙는 경우도 있다. We selected 638 weed species in the weed glossary book published by the Korean Society of Weed Science and linguistically studied them after categorizing them into different sizes, shapes, seasons, and associated animals insects, truths and lies, colors, persons, Similarities and relation with the five senses was also part of the categorization. The prefixes for size are narrow and/or small, long, small, little and/or bit, large and long. The prefixes for shape are thorn and/or needle, crawling/prostate, round and/or circular, stand, creases and/or folds and/or wrinkled and hairy. Some Korean weeds have names that personify animals such as dogs, cats, cows, rats and/or insects. Chambangdongsani (Cyperus iria) and Chamsorijaengi (Rumex japonicus) etc. are weeds that contain ‘real’ which means ‘truths’ and Gaegatnaengi (Rorippa indica), Gaegijang (Panicum bisulcatum) are weeds that contain ‘false’ which means ‘lies’ or ‘low qualities’. Some weeds contain the prefix ‘tideland’ which means reclaimed land among areas, plain or water which relates to water or waterside. Other troublesome exotic weeds have prefixes for U.S.A., western and/or Europe. Some weed names contain the appearance of the weed or the unique color of the flower such as yellow, red, purple, violet, and/or whitd. Among the weed, some weed names were personified as humans or degraded. Clown, Gisaeng, dwarf, fool are the human related subjects and designations as daughter-in-law. Then, some weeds contain the words ‘me too’ or ‘you too’ based on similarity.

An Early-Maturing, High-Quality, and High-Yielding Rice Cultivar "Munjangbyeo"

Kil Lim Ryu,Jeong IL Kim,You Chun Song,Jung Kon Kim,Beong Chul Moon,Sang Jong Lim,Ho Yeong Kim,Hae Chune Choi,Soon Chul Kim,Moo Sang Lim,Hee Woo Lee,Yeon Kyu Hong,Dae Sik Kim 한국육종학회 2003 한국육종학회지 Vol.35 No.1

Munjangbyeo' is a japonica rice cultivar developed from a cross between Sangsanbyeo and Suweon 397 by Sangju Substation of National Yeongnam Agricultural Experiment Station, RDA in 1999. The cultivar is early matured with heading date of Aug. 2 in ordinar

사람 양수중 다종의 세포외성 포스포리파제 A2의 부분정제 및 특성

전용주(Yong Ju Jeon),백석환(Suk Hwan Baek),이지혜(Jee Hae Lee),문태철(Tae Chul Moon),민병우(Beong Woo Min),장현욱(Hyeun Wook Chang) 대한약학회 1997 약학회지 Vol.41 No.2

Multiple forms of extracellular phospholipase A2 have been detected in human amniotic fluid (HAF). When HAF was subjected to heparin-Sepharose column chromatography, phospholipase A2 activity was detected in both heparin-non binding and binding fraction. The activity of heparin-non binding fraction was further purified by sequential uses of column chromatographies on butyl-Toy-opearl 650M and DEAE-Sephacel. DEAE-Sephacel fraction contained three different phospholipase A2 activities (Peak I, II, III). The molecular weight of DEAE-Sephacel fraction phospholipase A2 determined by SDS-PAGE were about 52KDa (Peak I). Peak II, III required micromolar Ca2+ ion for its maximum activity, but Peak I enzyme showed calcium independent phospholipase A2 activity and showed broad range of pH (6.0~10.0) optimum. All these enzymes were not recognized by a monoclonal antibody raised against phospholipase A2 from human synovial fluid. These results suggest that HAF might contain multiple forms of extracellular phospholipase A2, which may neither belong to the 14KDa group II phospholipase A2 family nor cytosolic phospholipase A2.