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들잔디 성숙종자로부터 캘러스배양 및 식물체 재분화에 미치는 몇 가지 요인의 영향
이상훈,김범수,원성혜,조진기,김기용,박근제,성병렬,이효신,이병현 Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-
In an effort to optimize tissue culture responses of zoysiagrass (Zoysia japonica Steud.) for genetic transformation, factors affecting callus induction and plant regeneration were investigated. MS medium containing 3 ㎎/L 2,4-D was optimal for embryogenic callus induction from mature seed. The plant regeneration frequency of 73.3% was observed when embryogenic calli induced in this medium were transferred to N6 medium supplemented with 0.1 ㎎/L 2,4-D and 5 ㎎/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Regenerated plants were grown normally when shoots transplanted to the soil. A rapid and efficient plant regeneration system established in this study will be useful for molecular breeding of turfgrass through genetic transformation.
Jo, Beom-Geun,Park, No-June,Jegal, Jonghwan,Choi, Sangho,Lee, Sang,Yi, Li,Kim, Su-Nam,Yang, Min Georg Thieme Verlag KG 2019 Planta medica Vol.85 No.7
<B>Abstract</B><P> Stellera chamaejasme, also known as “Langdu”, has been traditionally used for the management of skin-related diseases such as psoriasis and skin ulcers. The aim of this study was to determine whether S. chamaejasme and its major component, luteolin 7-O-glucoside, have a preventive effect on the development of atopic dermatitis in oxazolone-treated BALB/c mice and 2,4-dinitrochlorobenzene-treated hairless mice. The epicutaneous applications of oxazolone and 2,4-dinitrochlorobenzene evoke an experimental murine atopic dermatitis-like reaction in BALB/c mouse ears and SKH-1 hairless mice. Atopic skin symptoms, including erythema (redness), pruritus (itching), exudation (weeping), excoriation (peeling), and lichenification (skin thickening), responded to treatment with S. chamaejasme aerial parts EtOH extract for 2 or 3 weeks. Histopathological examination revealed S. chamaejasme aerial parts EtOH extract significantly reduced inflammatory cell infiltration when applied to atopic dermatitis mice. In addition, luteolin 7-O-glucoside, the major active compound of the S. chamaejasme aerial parts EtOH extract, decreased serum IgE and IL-4 levels and transepidermal water loss and increased skin hydration, therefore exhibiting strong anti-atopic dermatitis activity in 2,4-dinitrochlorobenzene-induced atopic dermatitis mice. In this study, we confirmed antipruritic and antidermatitic effects of S. chamaejasme extract and its main component luteolin 7-O-glucoside in atopic dermatitis murine models. The study shows S. chamaejasme aerial parts EtOH extract and luteolin 7-O-glucoside are most likely to be potential drug candidates for atopic dermatitis treatment.</P>
Jo, Beom-Geun,Park, No-June,Jegal, Jonghwan,Choi, Sangho,Lee, Sang Woo,Jin, Hang,Kim, Su-Nam,Yang, Min Hye Elsevier 2018 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.59 No.-
<P><B>Abstract</B></P> <P> <I>Stellera chamaejasme</I> L. (family Thymelaeaceae), also known as ‘<I>Langdu</I>’, has been traditionally used to treat of skin-related diseases, such as, psoriasis and skin ulcers. The aim of this study was to identify the biologically active component of <I>S</I>. <I>chamaejasme</I> and evaluate its preventive effects on IL-4 and mast cell degranulation in RBL-2H3 cells and on the development of atopic dermatitis (AD) in 2,4-dinitrochlorobenzene (DNCB)-treated SKH-1 hairless mice. A novel flavonoid, genkwanin 5-<I>O</I>-xylosyl(1 → 2)glucoside (named stechamone), and three known compounds (umbelliferone, luteolin, and luteolin-7-<I>O</I>-glucoside) were isolated from the aerial parts of <I>S</I>. <I>chamaejasme</I> using chromatographic methods. Of these four compounds, stechamone most potently inhibited IL-4 production and mast cell degranulation in RBL-2H3 cells. Topical application of 0.5% stechamone improved atopic skin symptoms, including, erythema (redness), pruritus (itching), exudation (weeping), excoriation (peeling), and lichenification (skin thickening) in DNCB-treated AD mice by accelerating skin barrier recovery function and suppressing inflammatory cell infiltration. In addition, stechamone attenuated DNCB-induced increases in IL-4 (an inflammatory T<SUB>H</SUB>2 cytokine) expression and in serum IgE levels in our murine model of AD. DNCB induced AD-like skin lesions, but treatment with stechamone exhibited strong anti-atopic activity by regulating skin barrier function and reducing inflammatory responses. The results obtained suggest stechamone is a potential anti-atopic agent and treatment for skin inflammatory diseases.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A new flavonoid, stechamone, was isolated from <I>Stellera chamaejasme</I>. </LI> <LI> Stechamone exhibited potent IL-4 inhibitory activity in RBL-2H3 cells. </LI> <LI> 2,4-dinitrochlorobenzene was used to induce atopic dermatitis (AD) in hairless mice. </LI> <LI> Stechamone appeared to exert strong anti-AD effects on DNCB-stimulated mice. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
류근관 ( Lyu Geun Gwan ),빈기범 ( Bin Gi Beom ),이영조 ( Lee Yeong Jo ),조성준 ( Jo Seong Jun ) 한국파생상품학회 2003 선물연구 Vol.11 No.1
Efficient market hypothesis implies that the past price movements do not help forecast future price movements. Thus, it is impossible to consistently benefit by a technical trading strategy. On the other hand. technical analysts claim that the historical price movements are useful in predicting future price movements. These two lines of arguments are mutually contradictory. This paper reasonably assumes that the more efficient markets are, the worse will be the investment performance of technical analysis, and that as financial market`s trading volume grows and with the elapse of time, the efficiency of markets should improve. This implies that after the launch of a new financial asset, market efficiency would improve with increased trading and elapsed time. In this paper, the duration analysis technique is used as a forecasting model and applied to measure the efficiency of Korean futures market and the won/dollar exchange rate market.