Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

Si₃N₄ 소결체의 미세조직과 파괴인성측정

최태운,김범석 慶星大學校 1998 論文集 Vol.19 No.2

Pure fine β-Si₃N₄powdersand fine β-Si₃N₄powders containing 3wt% large particles were hot-pressed at 1700℃ for 0hr using Y₂O₃and MgO as aids. These were gas-pressure annealed at 1800℃ for 0.5hr and 1hr, respectively. As a result of experiments, the following conclusions can be drawn. 1) When the fine and uniform β-Si₃N₄powders were sintered and annealed, the micro-structure has kept uniform. 2) When the fine β-Si₃N₄powders containing large particles were sintered and annealed, the needle-like particles were grown and the fracture toughness was increased with incleasing annealing time. 3) The fracture toughness(Kic) of annealing specimens was two times larger than that of hot-pressed specimens.

Isopiestic 증류법에 의한 염산의 정제

최범석 慶熙大學校 材料科學技術硏究所 1988 材料科學技術硏究論集 Vol.1 No.-

Hydrochloric acid used for rinsing and etching agents for semiconductor industry was purified by isopiestic distillation. Fifteen of the inorganic elements which appear to be critical for quality control were analyzed. The efficiency of purification was investigated by comparing the analytical data of purified products with the specifications of the electronic grade reagent. In order to prevent contaminations from air and containers in the preconcentration process necessary for the determination of ppb level impurities, sample was treated under the controlled system for contaimnation. Both of the atomic absorption and inductively coupled plasma spectrometries were employed for the determination of impurities.

Lutembacher 증후군 환자 증례 보고

김성범,서정주,곽철훈,김상민,이보라,민선경,황은구,김용인,조욱현,최석구 인제대학교 2008 仁濟醫學 Vol.29 No.-

Lutembacher's syndrome is rare combination of mitral stenosis (MS) and atrial septal defect (ASD). The hemodynamic interplay between the MS and ASD leads to wide variation of clinical presentation. Here we describe a 43-year-old female with Lutembacher's syndrome and tricuspid regurgitation with pulmonary hypertension, who underwent direct closure of ASD and tricuspid valvuloplasty successfully. We also reviewed other literatures in an effort to increase awareness of this condition.

Aluminum응집제들에 의한 금속이온들의 응집에 관한 연구

김미향,최범석 慶熙大學校 材料科學技術硏究所 1999 材料科學技術硏究論集 Vol.12 No.-

Coagulation efficiencies of inorganic ions by aluminum based coagulants including alum, PAC, PACS were studied. Pb, As, Cu, Hg ions shows good coagulation efficiences with all the coagulants. Residual amount of Hg is also decreased by the precipitation as a hydroxide. The maximum coaulation efficiency is observed at the neutral pH and the efficiency is decreased with lowering pH, except Se. The coagulation efficiencis are decresed with an order of PACS, PAC, and alum. Cr, Cd shows lower coagulation efficiencies and Ni and Mn are not coagulated with all the coagulants.

질병의 활동도 지표로써 머리카락 중 미량의 구리와 아연의 분석

정한성,최범석 慶熙大學校 材料科學技術硏究所 1997 材料科學技術硏究論集 Vol.10 No.-

The copper and zinc in scalp hair are determined by the ICP-AES and the relations between the trace elements concentrations with disease activity for the rheumatoid arthritis are investigated. The concentrations of the metals are varied depending upon the distance from the skin. The concentrations at the distal are higher than that of the proximal. Zinc concentration of the rheumatoid arthritis patients is higher than that of the osteroarthritis ones, and the confidence level that the group are different lies 95-99%. However, copper concentration for rheumatoid arthritis patients is lower and the two groups are different with a 50-80% confidence level.

Tributyl Phosphate와 질산염을 이용한 희토류원소의 용매추출 효과에 관한 연구

이정민,김숙영,최범석 경희대학교 자연과학종합연구원 2000 자연과학논문집 Vol.6 No.-

ICP-AES으로 희토류원소의 분석시 스펙트럼의 방해영향이 없는 분석을 시도하기 위하여 13종의 희토류 원소를 tributyl phdspate로 용매 추출하고 물로 역추출하고, 추출시 희토류원소의 추출효율을 조사하였다. 용매추출시 분배계수를 증가시키기 위하여 NaNO_(3) 농도에 따른 영향과, 소수성 용매의 첨가시 추출 효율을 조사하였다. NaNO_(3) 농도가 증가할수록 추출효율은 증가하며, 4M 이상에서 몇가지의 원소를 제외하고 추출효율이 95% 이상으로 높았다. 반면에 소수성용매의 첨가는 추출효율을 오히려 감소시켰다. Group separation of 13 rare earth elements(REE) was achieved by solvent extraction with tributyl phsphate, followed by back extraction with water in order to separate the REE from the matrix. The method can circumvent the spectral interferences for ICP-AES. Effects of the NaNO_(3) concentration and addition of hydrophobic solvents on the extraction efficiencies are investigated. Increases of the NaNO_(3) concentration enhanced the extraction efficiencies of REE and more than 95% recovery was obtained above 4 M of NaNO_(3) concentration. On the other hand, addition of hydrophobic solvents lowered the extraction efficiencies.

내시경적 점막절제술로 치험한 식도 과립상 세포종 1례

강혁주,김성욱,최석진,이중현,장재식,서영범,윤병구,박건욱,김성자,김용섭,강승완,이구,양창헌,이창우,김욱년,이광헌,서정일 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-

과립상 세포종은 Schwann 세포 기원으로 생각되며 인체에 비교적 드물게 발생한다. 과립상 세포종은 전신 어느 곳에서나 발견될 수 있으나 주로 혀, 구강, 피부 혹은 유방 등에서 호발하며 드물게 위장관에서 발견된다. 위장관에서는 식도에서 가장 호발하며 다음으로 위, 대장 순이다. 과립상 세포종은 대부분, 특히 위장관에서는 양성이며 소수의 악성 병변이 보고되었다. 이러한 이유와 함께 수술 전의 진단이 어렵기 때문에 과립상 세포종에 대한 근본적인 치료는 현재까지 외과적 절제술이다. 최근에 시도되는 치료방법들로는 내시경적 레이저 치료, 용종절제술, 내시경적 점막 절제술 등이 있다. 저자들은 상부 소화관 내시경검사를 시행하여 식도 과립상 세포종을 진단하고 내시경적 점막 절제술을 시행하여 합병증 없이 퇴원하여 현재 재발없이 경과 관찰중인 1례를 경험하였기에 보고하는 바이다. Granular cell tumors, which occur infrequently, are probably of Schwann cell origin. They can occur almost anywhere in the body but usually affect the tongue, oral cavity, skin, or breasts and are rarely found in the gastrointestinal tracts. The esophagus is the most frequent gastrointestinal site, followed by the stomach and the colon. Granular cell tumors are generally benign, especially in the gastrointestinal tract, some malignant lesions have been reported. For this reason, and also because preoperative diagnosis is difficult, the standard treatment for granular cell tumor has until now been surgical excision. In recent years, other therapeutic methods is endoscopic laser therapy (ELT), polypectiomy, endoscopic mucosal resection (EMR). We report a case of esophageal granular cell tumor which was diagnosed by an endoscopy and managed using an endoscopic mucosal resection without complication.

Determination of Germanium in Ganoderma Lucidum by Hydride Generation-ICP AES

Choi, Beom Suk,Kim, Sun Tae 경희대학교 환경연구소 1993 環境硏究 論文集 Vol.4 No.-

A method is described for the determination of germanium in ganoderma lucidum by hydride generation-inductively coupled plasma spectroscopy. The effect of several acids on the reduction of germanium to the hydride, and interelement interferences were investigated. Recovery tests with organic germanium were examined to ensure complete sample decomposition, and to avoid losses of volatile germanium compounds. Interelement interferences were overcome by the extraction of germanium into a mixture of 9M hydrochloric acid and carbon tetrachloride, followed by back extraction into water. Wet digestion with nitric, perchloric and sulfuric acids showed complete decomposition of ganoderma lucidum without loss of germanium.

FATIGUE LIFE ASSESSMENT OF REACTOR COOLANT SYSTEM COMPONENTS BY USING TRANSFER FUNCTIONS OF INTEGRATED FE MODEL

Choi, Shin-Beom,Chang, Yoon-Suk,Choi, Jae-Boong,Kim, Young-Jin,Jhung, Myung-Jo,Choi, Young-Hwan Korean Nuclear Society 2010 Nuclear Engineering and Technology Vol.42 No.5

Recently, efficient operation and practical management of power plants have become important issues in the nuclear industry. In particular, typical aging parameters such as stress and cumulative usage factor should be determined accurately for continued operation of a nuclear power plant beyond design life. However, most of the major components have been designed via conservative codes based on a 2-D concept, which do not take into account exact boundary conditions and asymmetric geometries. The present paper aims to suggest an effective fatigue evaluation methodology that uses a prototype of the integrated model and its transfer functions. The validity of the integrated 3-D Finite Element (FE) model was proven by comparing the analysis results of individual FE models. Also, mechanical and thermal transfer functions, known as Green's functions, were developed for the integrated model with the standard step input. Finally, the stresses estimated from the transfer functions were compared with those obtained from detailed 3-D FE analyses results at critical locations of the major components. The usefulness of the proposed fatigue evaluation methodology can be maximized by combining it with an on-line monitoring system, and this combination, will enhance the continued operations of old nuclear power plants.