Investigation of PCR-RFLPs within Major Histocompatibility Complex B-G Genes Using Two Restriction Enzymes in Eight Breeds of Chinese Indigenous Chickens

R. F. Xu,K. Li,G. H. Chen,B. Y. Z. Qiang,D. L. Mo,B. Fan,C. C. Li,M. Yu,M. J. Zhu,T. A. Xiong,B. Liu 아세아·태평양축산학회 2005 Animal Bioscience Vol.18 No.7

New polymorphism of major histocompatibility complex B-G genes was investigated by amplification and digestion of a 401bp fragment including intron 1 and exon 2 using polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) technique with two restriction enzymes of Msp I and Tas I in eight breeds of Chinese indigenous chickens and one exotic breed. In the fragment region of the gene, three novel single nucleotide polymorphisms (SNPs) were detected at the two restriction sites. We found the transition of two nucleotides of A294G and T295C occurred at Tas I restriction site, and consequently led to a nonsynonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variableregion- like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that a single mutation of A294G occurring at the site, also caused an identical substitution of amino acid, asparagine 54-to-serine, to that we described previously. And the transversion of G319C at Msp I site led to a non-synonymous substitution, glutamine 62-to-histidine. The new alleles and allele frequencies identified by the PCR-RFLP method with the two enzymes were characterized, of which the allele A and B frequencies at Msp I and Tas I loci were given disequilibrium distribution either in the eight Chinese local breeds or in the exotic breed. By comparison, allele A at Msp I locus tended to be dominant, while, the allele B at Tas I locus tended to be dominant in all of the breeds analyzed. In Tibetan chickens, the preliminary association analysis revealed that no significant difference was observed between the different genotypes identified at the Msp I and Tas I loci and the laying performance traits, respectively.

Paenibacillus harenae sp. nov., isolated from desert sand in China

Jeon, C. O.,Lim, J.-M.,Lee, S. S.,Chung, B. S.,Park, D.-J.,Xu, L.-H.,Jiang, C.-L.,Kim, C.-J. Microbiology Society 2009 International journal of systematic and evolutiona Vol.59 No.1

<P>A Gram-positive, endospore-forming, rod-shaped bacterium, designated strain B519T, was isolated from a desert sand sample of Gansu Province, China. Strain B519T was strictly aerobic and cells were motile by means of peritrichous flagella. The strain grew optimally at 32-35 degrees C and pH 6.5-7.0. Chemotaxonomic data supported the affiliation of the new isolate to the genus Paenibacillus, including menaquinone-7 (MK-7) as the major isoprenoid quinone, DNA G+C content of 49.9 mol%, cell-wall type A1gamma (meso-diaminopimelic acid as the diagnostic diamino acid) and anteiso-C(15 : 0), iso-C(15 : 0), C(16 : 0) and iso-C(16 : 0) as the major fatty acids. Comparative 16S rRNA gene sequence analysis showed that strain B519T was most closely related to Paenibacillus alkaliterrae KSL-134T (98.0 % similarity). DNA-DNA relatedness between strain B519T and P. alkaliterrae KSL-134T was about 12.3 %. On the basis of phenotypic characteristics and molecular properties, strain B519T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus harenae sp. nov. is proposed. The type strain is B519T (=KCTC 3951T =DSM 16969T).</P>

Kinetics of microbial hydrogenation of free linoleic acid to conjugated linoleic acids

Xu, H.,Lee, H.Y.,Hwang, B.,Nam, J.H.,Kang, H.Y.,Ahn, J. Blackwell Publishing Ltd 2008 Journal of applied microbiology Vol.105 No.6

<P>Abstract</P><P>Aims: </P><P>To investigate the ability of selected probiotic bacterial strains to produce conjugated linoleic acid (CLA) and also to estimate the biohydrogenation kinetics of <I>Lactobacillus acidophilus</I> on the production of CLA from free linoleic acid (LA).</P><P>Methods and Results: </P><P>Six probiotic bacteria, <I>Lact</I>. <I>paracasei</I>, <I>Lact</I>. <I>rhamnosus</I> GG, <I>Lact</I>. <I>acidophilus</I> ADH, and <I>Bifidobacterium longum</I> B6, <I>Lact</I>. <I>brevis</I>, and <I>Lact</I>. <I>casei</I>, were used to examine their ability to convert LA to CLA. LA tolerance was evaluated by addition of different LA concentrations in MRS broth. <I>Lact</I>. <I>acidophilus</I> showed the major tolerant to LA and the greatest CLA-producing ability (36–48 &mgr;g ml<SUP>−1</SUP> of CLA). The rate-controlling steps were <I>k</I><SUB>2</SUB> and <I>k</I><SUB>1</SUB> for the addition of 1 and 3 mg ml<SUP>−1</SUP> of LA, respectively. The percentage of CLA conversion was higher in MRS broth supplemented with 1 mg ml<SUP>−1</SUP> (65%) than 3 mg ml<SUP>−1</SUP> (26%).</P><P>Conclusion: </P><P>The results provide useful information and new approach for understanding the biohydrogenation mechanisms of CLA production.</P><P>Significance and Impact of the Study: </P><P>This study would help elucidate the pathway from LA to stearic acid (SA), known as biohydrogenation. In addition, the use of selected probiotic bacteria might lead to a significant improvement in food safety.</P>

Numerical simulation of fully nonlinear sloshing waves in three-dimensional tank under random excitation

Xu, Gang,Hamouda, A.M.S.,Khoo, B.C. Techno-Press 2011 Ocean systems engineering Vol.1 No.4

Based on the fully nonlinear velocity potential theory, the liquid sloshing in a three dimensional tank under random excitation is studied. The governing Laplace equation with fully nonlinear boundary conditions on the moving free surface is solved using the indirect desingularized boundary integral equation method (DBIEM). The fourth-order predictor-corrector Adams-Bashforth-Moulton scheme (ABM4) and mixed Eulerian-Lagrangian (MEL) method are used for the time-stepping integration of the free surface boundary conditions. A smoothing scheme, B-spline curve, is applied to both the longitudinal and transverse directions of the tank to eliminate the possible saw-tooth instabilities. When the tank is undergoing one dimensional regular motion of small amplitude, the calculated results are found to be in very good agreement with linear analytical solution. In the simulation, the normal standing waves, travelling waves and bores are observed. The extensive calculation has been made for the tank undergoing specified random oscillation. The nonlinear effect of random sloshing wave is studied and the effect of peak frequency used for the generation of random oscillation is investigated. It is found that, even as the peak value of spectrum for oscillation becomes smaller, the maximum wave elevation on the side wall becomes bigger when the peak frequency is closer to the natural frequency.

Wave propagation in a 3D fully nonlinear NWT based on MTF coupled with DZ method for the downstream boundary

Xu, G.,Hamouda, A.M.S.,Khoo, B.C. Techno-Press 2014 Ocean systems engineering Vol.4 No.2

Wave propagation in a three-dimensional (3D) fully nonlinear numerical wave tank (NWT) is studied based on velocity potential theory. The governing Laplace equation with fully nonlinear boundary conditions on the moving free surface is solved using the indirect desingularized boundary integral equation method (DBIEM). The fourth-order predictor-corrector Adams-Bashforth-Moulton scheme (ABM4) and mixed Eulerian-Lagrangian (MEL) method are used for the time-stepping integration of the free surface boundary conditions. A smoothing algorithm, B-spline, is applied to eliminate the possible saw-tooth instabilities. The artificial wave speed employed in MTF (multi-transmitting formula) approach is investigated for fully nonlinear wave problem. The numerical results from incorporating the damping zone (DZ), MTF and MTF coupled DZ (MTF+DZ) methods as radiation condition are compared with analytical solution. An effective MTF+DZ method is finally adopted to simulate the 3D linear wave, second-order wave and irregular wave propagation. It is shown that the MTF+DZ method can be used for simulating fully nonlinear wave propagation very efficiently.

Impact of fatty liver on long-term outcomes in chronic hepatitis B: a systematic review and matched analysis of individual patient data meta-analysis

Yu Jun Wong,Vy H. Nguyen,Hwai-I Yang,Jie Li,Michael Huan Le,Wan-Jung Wu,Nicole Xinrong Han,Khi Yung Fong,Elizabeth Chen,Connie Wong,Fajuan Rui,Xiaoming Xu,Qi Xue,Xin Yu Hu,Wei Qiang Leow,George Boon-B 대한간학회 2023 Clinical and Molecular Hepatology(대한간학회지) Vol.29 No.3

Background/Aims: Chronic hepatitis B (CHB) and fatty liver (FL) often co-exist, but natural history data of this dual condition (CHB-FL) are sparse. Via a systematic review, conventional meta-analysis (MA) and individual patient-level data MA (IPDMA), we compared liver-related outcomes and mortality between CHB-FL and CHB-no FL patients. Methods: We searched 4 databases from inception to December 2021 and pooled study-level estimates using a random- effects model for conventional MA. For IPDMA, we evaluated outcomes after balancing the two study groups with inverse probability treatment weighting (IPTW) on age, sex, cirrhosis, diabetes, ALT, HBeAg, HBV DNA, and antiviral treatment. Results: We screened 2,157 articles and included 19 eligible studies (17,955 patients: 11,908 CHB-no FL; 6,047 CHB-FL) in conventional MA, which found severe heterogeneity (I2=88–95%) and no significant differences in HCC, cirrhosis, mortality, or HBsAg seroclearance incidence (P=0.27–0.93). IPDMA included 13,262 patients: 8,625 CHB-no FL and 4,637 CHB-FL patients who differed in several characteristics. The IPTW cohort included 6,955 CHB-no FL and 3,346 CHB-FL well-matched patients. CHB-FL patients (vs. CHB-no FL) had significantly lower HCC, cirrhosis, mortality and higher HBsAg seroclearance incidence (all P≤0.002), with consistent results in subgroups. CHB-FL diagnosed by liver biopsy had a higher 10-year cumulative HCC incidence than CHB-FL diagnosed with non-invasive methods (63.6% vs. 4.3%, P<0.0001). Conclusions: IPDMA data with well-matched CHB patient groups showed that FL (vs. no FL) was associated with significantly lower HCC, cirrhosis, and mortality risk and higher HBsAg seroclearance probability.

The Kinetics of Anodic Dissolution and Repassivation on 316L Stainless Steel in Borate Buffer Solution Studied by Abrading Electrode Technique

( H S Xu1 ),( D B Sun ),( H Y Yu ),( H M Meng ) 한국부식방식학회(구 한국부식학회) 2015 Corrosion Science and Technology Vol.14 No.6

The capacity of passive metal to repassivate after film damage determines the development of local corrosion and the resistance to corrosion failures. In this work, the repassivation kinetics of 316L stainless steel (316LSS) was investigated in borate buffer solution (pH 9. 1) using a novel abrading electrode technique. The repassivation kinetics was analyzed in terms of the current density flowing from freshly bare 316L SS surface as measured by a potentiostatic method. During the early phase of decay (t < 2 s), according to the Avrami kinetics-based film growth model, the transient current was separated into anodic dissolution (idiss) and film formation (ifilm) components and analyzed individually. The film reformation rate and thickness were compared according to applied potential. Anodic dissolution initially dominated the repassivation for a short time, and the amount of dissolution increased with increasing applied potential in the passive region. Film growth at higher potentials occurred more rapidly compared to at lower potentials. Increasing the applied potential from 0 VSCE to 0. 8 VSCE resulted in a thicker passive film (0. 12 to 0. 52 nm). If the oxide monolayer covered the entire bare surface (θ=1), the electric field strength through the thin passive film reached 1. 6 × 107 V/cm.

Ixabepilone Plus Capecitabine for Metastatic Breast Cancer Progressing After Anthracycline and Taxane Treatment

Thomas, Eva S.,Gomez, Henry L.,Li, Rubi K.,Chung, Hyun-Cheol,Fein, Luis E.,Chan, Valorie F.,Jassem, Jacek,Pivot, Xavier B.,Klimovsky, Judith V.,de Mendoza, Fernando Hurtado,Xu, Binghe,Campone, Mario,L Grune & Stratton 2007 Journal of clinical oncology Vol.25 No.33

<B>Purpose</B><P>Effective treatment options for patients with metastatic breast cancer resistant to anthracyclines and taxanes are limited. Ixabepilone has single-agent activity in these patients and has demonstrated synergy with capecitabine in this setting. This study was designed to compare ixabepilone plus capecitabine versus capecitabine alone in anthracycline-pretreated or -resistant and taxane-resistant locally advanced or metastatic breast cancer.</P><B>Patients and Methods</B><P>Seven hundred fifty-two patients were randomly assigned to ixabepilone 40 mg/m<SUP>2</SUP>intravenously on day 1 of a 21-day cycle plus capecitabine 2,000 mg/m<SUP>2</SUP>orally on days 1 through 14 of a 21-day cycle, or capecitabine alone 2,500 mg/m<SUP>2</SUP>on the same schedule, in this international phase III study. The primary end point was progression-free survival evaluated by blinded independent review.</P><B>Results</B><P>Ixabepilone plus capecitabine prolonged progression-free survival relative to capecitabine (median, 5.8 v 4.2 months), with a 25% reduction in the estimated risk of disease progression (hazard ratio, 0.75; 95% CI, 0.64 to 0.88; P = .0003). Objective response rate was also increased (35% v 14%; P < .0001). Grade 3/4 treatment-related sensory neuropathy (21% v 0%), fatigue (9% v 3%), and neutropenia (68% v 11%) were more frequent with combination therapy, as was the rate of death as a result of toxicity (3% v 1%, with patients with liver dysfunction [≥ grade 2 liver function tests] at greater risk). Capecitabine-related toxicities were similar for both treatment groups.</P><B>Conclusion</B><P>Ixabepilone plus capecitabine demonstrates superior efficacy to capecitabine alone in patients with metastatic breast cancer pretreated or resistant to anthracyclines and resistant to taxanes.</P>

Microwave Absorption and Magnetic Properties of Cobalt Ferrites/Carbon Nanotubes Nanocomposites

B. B. Zhang,P. F. Wang,J. C. Xu,Y. B. Han,H. X. Jin,D. F. Jin,X. L. Peng,B. Hong,J. Li,J. Gong,H. L. Ge,Z. W. Zhu,X. Q. Wang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.5

Owing to the unique microstructure and the excellent dielectric properties, carbon nanotubes(CNTs) were decorated with CoFe2O4 nanoparticles to synthesize the CoFe2O4/CNTs nanocomposites by the solvothermal method. The phase structure, morphology, magnetic properties and microwave absorption performance of the as-prepared CoFe2O4/CNTs were characterized and discussed by X-ray diffraction (XRD), thermal gravity analysis (TGA), transmission electron microscope (TEM), vibrating sample magnetometer (VSM) and vector network analyzer (VNA). All results indicated that the diameter of CoFe2O4 nanoparticles decorating on the surface of CNTs increased with the solvothermal temperature. CoFe2O4/CNTs prepared at 180℃, 200℃ and 220℃ exhibited superparamagnetism, while the other samples presented ferromagnetism at room temperature. And with the increasing solvothermal temperature, the saturation magnetization and coercivity increased up to 72 emu/g and 2000 Oe for the sample prepared at 260℃ (S-26). And the reflection loss of CoFe2O4/CNTs nanocomposites increased with the solvothermal temperature up to -15.7 dB for S-26 with the bandwidth of 2.5 GHz.

Effect of CoO additive on structure and electrical properties of (Na_0.5Bi_0.5)_0.93Ba_0.07TiO₃ ceramics prepared by the citrate method

Xu, Q.,Chen, M.,Chen, W.,Liu, H.X.,Kim, B.H.,Ahn, B.K. Elsevier Science 2008 ACTA MATERIALIA Vol.56 No.3

(Na<SUB>0.5</SUB>Bi<SUB>0.5</SUB>)<SUB>0.93</SUB>Ba<SUB>0.07</SUB>TiO<SUB>3</SUB> ceramics with added 0-0.8 wt.% CoO were prepared by a citrate method and the influence of the CoO additive on the structure and electrical properties of the ceramics was investigated. All the specimens maintained a rhombohedral-tetragonal phase coexistence in crystal structure and the addition of CoO caused a remarkably promoted grain growth. Adding CoO led to a disappearance of the response in the dielectric constant (ε<SUB>r</SUB>) to the ferroelectric-antiferroelectric transition and increased the diffuseness of the dielectric constant peak around 230<SUP>o</SUP>C. Polarization-electric field hysteresis loops at varied temperatures revealed that adding CoO served to increase the depolarization temperature (T<SUB>d</SUB>). The addition of CoO tailored the dielectric, piezoelectric and ferroelectric properties at room temperature basically following a hard doped effect. The specimen with 0.8 wt.% CoO added showed a low dissipation factor (tanδ) of 0.8% and a high mechanical quality factor (Q<SUB>m</SUB>) of 297 while retaining a piezoelectric constant (d<SUB>33</SUB>) of 137 pC N<SUP>-1</SUP>.