Comparative and Structural Analysis of the Interaction between β-Lactoglobulin type A and B with a New Anticancer Component (2,2'-Bipyridin n-Hexyl Dithiocarbamato Pd(II) Nitrate)

A. Divsalar,A. A. Saboury*,H. Mansoori-Torshizi,B. Hemmatinejad 대한화학회 2006 Bulletin of the Korean Chemical Society Vol.27 No.11

The interaction between whey carrier protein b-lactoglobulin type A and B (BLG-A and -B) and 2,2'-bipyridin n-hexyl dithiocarbamato Pd(II) nitrate (BPHDC-Pd(II)), a new heavy metal complex designed for anticancer property, was investigated by fluorescence spectroscopy combined with chemometry and circular dichroism (CD) techniques. A strong fluorescence quenching reaction of BPHDC-Pd(II) to BLG-A and -B was observed. Hence, BPHDC-Pd(II) complex can be bound to both BLG-A and -B, and quench the fluorescence spectra of the proteins. The quenching constant was determined using the modified Stern-Volmer equation. The binding parameters were evaluated by fluorescence quenching method. The results of binding study provided evidences presence of two and three sets of binding sites on the BLG-B and -A, respectively, for BPHDC-Pd(II) complex. Using fluorescence spectroscopy and chemometry, the ability of BLG-A and -B to form an intermediate upon interaction with BPHDC-Pd(II) complex was assessed. CD studies displayed that under influence of different concentrations of BPHDC-Pd(II) complex, the regular secondary structure of BLG-B had no significant changes, whereas for BLG-A a transition from a-helix to b-structure was appeared. The results for both of BLG-A and -B displayed that BPHDC-Pd(II) complex can induce a conformational transition from the native form to an intermediate state with a slightly opened conformation, which is detectable with chemometry analyses.

Adenosine triphosphate-based chemotherapy response assay-guided chemotherapy in unresectable colorectal liver metastasis

Hur, H,Kim, N K,Kim, H G,Min, B S,Lee, K Y,Shin, S J,Cheon, J H,Choi, S H Nature Publishing Group 2012 The British journal of cancer Vol.106 No.1

<P><B>Background:</B></P><P>This study aims to evaluate the effectiveness of adenosine triphosphate-based chemotherapy response assay (ATP-CRA)-guided neoadjuvant chemotherapy for increasing resectability in patients with unresectable colorectal liver metastasis.</P><P><B>Patients and methods:</B></P><P>Patients were randomised into two groups: Group A was treated by conventional chemotherapy regimen and Group B was treated by chemotherapy regimen according to the ATP-CRA. Three chemotherapeutic agents (5-fluorouracil, oxaliplatin and irinotecan) were tested by ATP-CRA and more sensitive agents were selected. Either FOLFOX or FOLFIRI was administered. Between Group A and B, treatment response and resectability were compared.</P><P><B>Results:</B></P><P>Between November 2008 and October 2010, a total 63 patients were randomised to Group A (<I>N</I>=32) or Group B (<I>N</I>=31). FOLFOX was more preferred in Group A than in Group B (26 out of 32 (81.3%) <I>vs</I> 20 out of 31 (64.5%)). Group B showed better treatment response than Group A (48.4% <I>vs</I> 21.9%, <I>P</I>=0.027). The resectability of hepatic lesion was higher in Group B (35.5% <I>vs</I> 12.5%, <I>P</I>=0.032). Mean duration from chemotherapy onset to the time of liver resection was 11 cycles (range 4–12) in Group A and 8 cycles (range 8–16) in Group B.</P><P><B>Conclusion:</B></P><P>This study showed that tailored-chemotherapy based on ATP-CRA could improve the treatment response and resectability in initially unresectable colorectal liver metastasis.</P>

Morphological, ecological and biological variations in the mustard aphid, Lipaphis pseudobrassicae (Kaltenbach) (Hemiptera: Aphididae) from different host plants

B.K. Agarwala,Kalpana Das,Parichita Raychoudhury 한국응용곤충학회 2009 Journal of Asia-Pacific Entomology Vol.12 No.3

Genetic and morphological differentiation of insect populations in relation to the use of different host plants is an important phenomenon that leads to ecological specialization. In this study, we describe variations in morphology, and in ecological and biological parameters of Lipaphis pseudobrassicae (Kaltenbach) clones associated with three host species of Cruciferae, Brassica juncea (L.) var. rai sarson Czern and Cross (brown mustard), Brassica campestris L. var. sarson Prain (yellow mustard), and Rorippa indica (L.) Hiern (wild herb). This study was aimed at obtaining evidence regarding phenotypic differentiation induced by, or associated with, the use of distinct host species. Ten morphological characters, 4 growth parameters and 8 biological functions were investigated in wingless aphids collected from plants of the three host species. Aphids from B. campestris and B. juncea clones were bigger in size, heavier in weight and showed higher growth rates and fecundity than the clones from R. indica. Between the two crop plants, clones from B. juncea showed significantly higher growth rates than the clones from B. campestris. Transfer of L. pseudobrassicae populations from B. campestris to B. juncea and R. indica and vice versa resulted in poor performance. Results indicate that the average phenotype of L. pseudobrassicae individuals inhabiting different host plant species differs as a consequence of the contrasting feeding environments the host species provide. Genetic and morphological differentiation of insect populations in relation to the use of different host plants is an important phenomenon that leads to ecological specialization. In this study, we describe variations in morphology, and in ecological and biological parameters of Lipaphis pseudobrassicae (Kaltenbach) clones associated with three host species of Cruciferae, Brassica juncea (L.) var. rai sarson Czern and Cross (brown mustard), Brassica campestris L. var. sarson Prain (yellow mustard), and Rorippa indica (L.) Hiern (wild herb). This study was aimed at obtaining evidence regarding phenotypic differentiation induced by, or associated with, the use of distinct host species. Ten morphological characters, 4 growth parameters and 8 biological functions were investigated in wingless aphids collected from plants of the three host species. Aphids from B. campestris and B. juncea clones were bigger in size, heavier in weight and showed higher growth rates and fecundity than the clones from R. indica. Between the two crop plants, clones from B. juncea showed significantly higher growth rates than the clones from B. campestris. Transfer of L. pseudobrassicae populations from B. campestris to B. juncea and R. indica and vice versa resulted in poor performance. Results indicate that the average phenotype of L. pseudobrassicae individuals inhabiting different host plant species differs as a consequence of the contrasting feeding environments the host species provide.

EFFECTS OF DIETARY VITAMIN B₆ LEVELS ON LIPID CONCENTRATION AND FATTY ACID COMPOSITION IN GROWING CHICKS

An, B.K.,Tanaka, K.,Ohtani, S. Asian Australasian Association of Animal Productio 1995 Animal Bioscience Vol.8 No.6

This experiment was designed to evaluate the effect of various dietary vitamin $B_6$ levels on conversion from linoleic acid to arachidonic acid in various tissues in growing chicks. Growing chicks were fed the purified diet containing 7% safflower oil with different levels of vitamin $B_6$ (0, 4, 8, 40, 80 mg per kg diet) for 14 days. Feed intake and weight gain in chicks fed the vitamin $B_6$-free diet were markedly depressed. Esterified and free cholesterol concentrations in serum were significantly higher, while the serum triglyceride concentration was significantly lover in chicks fed the vitamin $B_6$-free diet compared to that fed diets with vitamin $B_6$. The liver triglyceride content was also lower in chicks fed the vitamin $B_6$-free diet. The liver and serum cholesterol ester fractions in chicks fed the vitamin $B_6$-free diet showed higher rate of $C_{18:2n6}$ and lower rates of $C_{18:3n6}$, $C_{20:3n6}$ and $C_{20:4n6}$ as compared with vitamin $B_6$ fed groups. In serum phospholipid fraction of chicks fed the vitamin $B_6$-free diet, rates of $C_{20:3n6}$ and $C_{20:4n6}$ were markedly lower. As dietary vitamin $B_6$ level was increased, the rate of $C_{20:4n6}$ was slightly increased, although it was statistically not significant. The fatty acid compositions of adipose tissue showed almost the same pattern as those in liver and serum. This result suggests that the desaturation of $C_{18:2n6}$ to $C_{18:3n6}$, elongation to $C_{20:3n6}$ or both steps might be impaired by vitamin $B_6$ deficiency in growing chicks.

反芻胃 Celluloytic Bacteria에 의한 섬유소 분해 및 비구조 탄수화물의 이용성에 관한 硏究

민병렬,김범석,맹원재 건국대학교 동물자원연구센터 1991 動物資源硏究誌 Vol.16 No.-

본 실험은 반추위내에 존재하는 주요한 cellulolytic bacteria를 選擇 및 非選擇培地를 사용하여 면양의 rumen내에 존재하는 celluloytic bacteria를 사용하여 纖維素 分解菌 각각의 纖維素源의 分解度, 비구조 탄수화물의 이용성 및 휘발성 지방산의 생성량을 측정하였다. 본 실험결과를 요약해 보면 다음과 같다. 1) In vitro상에서 Corn stover, alfalfa, rice straw 및 filterpaper No.1등의 섬유소원에 대한 R.flavefaciens의 평균 DM 소화율은 10.75%로 가장 높은 소화율을 나타내었으며, B.succinogenes, Buty. fibrisolvens 및 B. ruminicola subsp. 등은 각각 8.20%, 5.66% 및 2.5%의 DM소화율을 나타내었다.(p<0.05). 2) 섬유소원에서의 평균 microbial dry cell生成量은 R.flavefaciens. B.succinogenes, Buty.fibrisolvens 및 B.rumminicola subsp. 등이 각각 11.4, 4.47, 6.38 및 1.13mg/100ml를 가장 높은 미생물 cell의 생성량을 나타내었다. 3) ADF 및 NDF 평균 소화율은 R.flavefaciens가 가장 높았으며(23.61% 및 39.87%), B.succinogenses는 각각 11.16% 및 35.91%의 분해도를 나타내었다. Buty. fibrisolvens는 16.82% 및 24.34%로서 가장 낮은 분해도를 나타내었다. CEllulose消化率은 B.succinogenes가 14.43%로 R.flavefaciens(11.11%)보다 높은 분해율을 나타내었다. 4) 비구조 炭水化物 利用率은 (OD=670nm)R.flavefaciens가 mannose, arabinose 및 xylose 등을 가장 잘 이용하며, B. ruminicola subsp.은 galactose, mannose, arabinose 및 xylose등을 가장 잘 이용하는 것으로 나타났다. B.succinogenes는 glucose 및 rffainose를 가장 잘 이용하며, B.fibrisolvens는 glucose, galactose, arabinose, xylose 및 maltose등을 가장 잘 이용하는 것으로 나타났다. (P<0.05). 5) PYG basal medium에 炭水化物源을 각각 1g씩 첨가한 培養區에서 VFA 생성량은 R.flavefaciens는 주로 acetate, propionate, n-butyrate 및 succinate를 생산하는 것으로 나타났으며, 이것들의 생산량은 각각 81.65, 26.36, 20.16 및 108.91mM/100ml를 생산하여 succinate를 가장 많이 생산하는 것으로 나타났다. B.ruminicola subsp은 주로 acetate, propionate 및 n-butyrate를 생산하는 것으로 나타났으며, 이 균의 생산량은 각각 104.72, 31.32 및 26.12mM/100ml를 생산하였으며, B. succinogenes는 주로 acetate, propionate, n-butyrate 및 succinate를 생산하며, 이 균들의 생산량은 각각 87.66, 27.70 및 56.07mM/100ml 생산하였다. Buty.fibrisolvens는 주로 acetate, propionate 및 n-butyrate를 생산하는 것으로 나타났다. Fiber degradation of corn stover, rice straw, alfalfa or filter paper No.1 was evaluated using microscopy and in vitro analyses after incubation with pure culture of cellulolytic ruminal bacteria. 1) Analyses of the initial substrate and the recovered residue after 24h of static incubation showed that R.flavefaciens degraded a mean dry matter(10.75% versus 5.66%) more effectively than B. succinogenes. However, B, succinogenes demonstrated a qualitative advantage in degrading cellulose of particular substrates. DM digestibilities for alfalfa and rice straw. Treatment for 24h were 13.96% and 9.46% for R.lflavefaciens, 10.27% and 4.32% for B.succinogenes, and 7.74% and 2.31% for Buty. fibrisolvens(p<0.05). Bacteriodes ruminitola subsp. was unable to degrade plant cell wall components, while it degraded non-structural carbohydrates more actively than any other bacteria. Microbial cell DM yield(avg, 11.44mg/dl) was the highest for R. flavefaciens cultured with alfalfa substrate(p<0.05). 2) Ruminococcu flavefaciens degraded a mean 23.61% of the ADF and 39.87% of the NDF in the various substrates. whereas, B. succinogenes degraded a mean 11.6% and 35.91% of these fractions, respectively. 3) The utilization of carbodydrates was the highest for R.flavefaciens cultured with mannose and xylcse substrate, and for B. ruminicola subsp. with arabinose, xylose, raffinose and galactose respectively. B. succinogenes utilized glucose and raffinose, as major substrates fermentd(p<0.05) and Buty. fibrisolvens utilized glucose, galactose, arabinose, xylose and maltose. 4) Ruminococcus flavefaciens produced acetate, succinate and propionate, and B.ruminicola subsp. produced acetate, propionate and n-butyrate as major VFA. B.succinogenes produced acetate, propionate, n-butyrate and succinate, and Buty.fibrisolvens produced n-butyrate and acetate as major VFA.

On the range kernel orthogonality of derivations

Duggal, B.P. TOPOLOGY AND GEOMETRY RESEARCH CENTER 1998 Proceedings of the Topology and Geometry Research Vol.9 No.-

Let H be a separable infinite dimensional complex Hilbert space, and let B(H) denote the algebra of operators on H into itself. The generalized derivation δA,B : B(H) → B(H) is defined by δA,B (X) = AX - XB; let △A,B : B(H) → B(H) be defined by △A,B (X) = AXB - X, and let d A,B denote δA,B or △A,B . Given that S ∈ Cp (the Schatten p-class, 1 < p < ∞) and that the pair of operators A and B* satisfies a Putnam-Fuglede commutativity theorem type property, we consider here a necessary and sufficient condition for ∥dA,B (X) + S∥p ≥ ∥S∥ p to hold for all X ∈ C p.

반추위내 (反芻胃內) Cellulolytic bacteria 의 분리 동정 (同定) 및 Activity 에 관한 연구 (2) 반추위내 Cellulolytic bacteria 의 Activity 에 관한 연구

민병렬(B . R . Min),맹원재(W . J . Maeng),조남기(N . K . Cho) 한국영양사료학회 1991 韓國營養飼料學會誌 Vol.15 No.3

국내 감염성질환 병원체의 성상분석을 통한 표준실험실 체계 구축(Ⅱ) : 장내바이러스와 B형간염바이러스 국내 분리주의 성상분석(1)

윤재득,지영미,김기순,천두성,안정배,정윤석,신수연,이선화,이지원,조해월 국립보건연구원 2000 국립보건원보 Vol.37 No.-

1998년 분리된 이질균의 역학적 특성

이복권,강연호,김성한,유재연,전정훈 국립보건연구원 1998 국립보건원보 Vol.35 No.-

박 보리스의 생애와 한국학 연구

박벨라보리소브나 ( Bella B. Pak ) 인하대학교 한국학연구소 2013 한국학연구 Vol.0 No.29

This article attempts to describe the life and activity of the outstanding scientist of Korean studies Boris Dmitrievich Pak, major milestones of his career in science, shaping him as a scholar, an important focus of his research, the most important works. assess his contribution to Korean studies, to the forming the new generation of scientists, his social activities, to show his place in the domestic and the international scientific community, to tell about a time when he had to live and work, the difficulties that he as a son of the “enemy of the people” and a representative of the repressed people had to overcome. The author also wanted in addition to the analysis of B. D. Pak ‘s works to help to get to know him, to reveal some features of his personality, human side, based on her personal experiences. The article describes the childhood years, deportation, education in the Central State University, postgraduate studies, the first monograph. It described period after the move to Moscow and work at the Institute of Oriental Studies, analyzes his main works. The author gives the characteristic of the school of B. D. Pak, his international and social activities. Boris D. Pak was born on January 4th, 1931 in Vladivostok, in the family of Pak Dynnen and Choi Yon Ai. In 1937, his father was arrested “as a Japanese spy” and shot. He was posthumously rehabilitated in 1958. Boris’s family survived the deportation of the Korean population in Kazakhstan and Central Asia. Being a “son of an enemy of the people” B. D. Pak from the early years experienced enormous hardships, was forced to do hard physical labor. But despite this he graduated from high school with honors in 1950 and joined the faculty of the Eastern Central Asian State University in Tashkent, as a history major. Already during his studies at the university he showed a great aptitude for scientific research. He took part in student conferences. His paper on the Japanese Communist Party in 1954, was published in the proceedings of the students. The collection opened with his article. Undoubted leadership qualities, excellent achievements in learning, talent, and a great capacity for work soon attracted attention and he was elected leader of the Young Communist League. After graduating with honors in 1956, B. D. Pak worked as a teacher of history and director of the school № 13 with the famous collective farm “Polar Star” in Middle-Chirchik district of Tashkent region. In 1962, Boris D. Pak enrolled in postgraduate studies at Moscow State Pedagogical Institute named after V. I. Lenin. This became possible with the death of Stalin, when for the Koreans in Central Asia and Kazakhstan the restrictions on studying and living outside the Central Asian republics were eased. In 1965 he defended his Ph. D. thesis “The liberation struggle of the Korean people on the eve of the First World War.” In 1967, it was published by the publishing house “Science”. After finishing graduate school, B. D. Pak was sent as a lecturer in the history of the East to Irkutsk State Pedagogical Institute. The most prolonged and intense “Siberian period” in the scientific activities of Boris Pak began. In September 1968, Boris Dmitrievich received academic rank of assistant professor and was elected Dean of the Faculty of History. In 1972, B. D. Pak enrolled in doctoral studies at Moscow State Pedagogical University, and in 1974 he defended his doctoral thesis on “Russia and Korea in the middle of XIX - early XX century.” After that, he was elected chair of World History. In 1977 he was awarded the title of professor. He becomes the leading scientist in Siberia on Korea and East Asia. B. D. Pak is the author of over 200 scientific papers. His writings on the history of Russian-Korean relations, the history of Russian Koreans recognized classic, translated into many languages. Boris D. Pak is the founder of the trend in Korean Studies - History of Russian-Korean relations. His book “Russia and Korea” examines Russia's policy in Korea, the Russian-Korean relations since their inception in the thirteenth century until the annexation of Korea in 1910. A major landmark in his work since the mid 90's was the study of Korean anti-Japanese national liberation movement. On this issue he wrote his monographs, “the March First Movement in 1919 in Korea through the eyes of a Russian diplomat (1997)”(in collaboration with Pak Thegyn) and “The Recompence in the Harbin Railway Station”(1998) - the first monographic study of the An Junggeun. The name Boris Dmitrievich Pak linked with the development of the history of Russian Koreans as a scientific discipline. On this issue in the second half of the 90's he published the books: “The Koreans in the Russian Empire”(1994), “Koreans in Soviet Russia”(1995). They first examined the history of Korean immigration to the Russian Empire since the 50's XIX century up to 1937. From October 1999 until his death on December 24, 2012 B. D. Pak worked in the Department of Korea and Mongolia of the Institute of Oriental Studies Russian Academy of Sciences. In creative terms, these years have been extremely fruitful in the life of B. D. Pak. Written with Ninkolay F. Bugai the book “140 years in Russia. Essay on the history of Russian Koreans”(Moscow, 2004), dedicated to the 140 anniversary of the resettlement of Koreans in Russia, has been translated into Korean and became one of the most important works on the history of Russian Koreans. In 2006, Boris Dmitrievich Pak has published the book “USSR, the Communist International and the Korean liberation movement. 1918~1925”. B. D. Pak is the founder of the series of monographs “Russian Koreans” dedicated to outstanding representatives of the diaspora of Russian Koreans. He is the author-compiler and editor of books in this series(“Kim Mangym”, “Khan Myonse”, “Lee Bomjin”, “140 years in Russia”(in co. with N.F. Bugai), “Kim Pen Hwa”, “Alexandra Kim-Stankevich”, “Choi Jaehyen”, “Russian Koreans struggle for the independence of Korea. 1905~1919”, “Soviet Koreans in the Great Patriotic War of 1941~1945”). Within this series first appeared detailed biographies of the most prominent representatives of Koreans in prerevolutionary period and Soviet era, including those who have been subjected to repression during the Stalinist era. Great work led B. D. Pak and as archivist. Together with Y. V. Vanin prepared for publication, and was editor of the compiler of collections of documents and materials “Korea through the eyes of Russians, 1895~1945” and “The first news about Korea. 1675~1884”. B. D. Pak contributed greatly to the scientific training of historians. Under his leadership, 12 candidates and doctors of history were prepared. B. D. Pak contributed to the development and emergence in Russia, Korea, Japan and China of publications, which have based their approaches on the principles laid down by him. Attention to historical sources, the highest professionalism in their study are a distinctive feature of his research school. B. D. Pak created the International Centre for Asian Studies at the Irkutsk State Pedagogical University, that become one of the most famous centers for the study of Asia in Siberia. He developed the International relations of Irkutsk Pedagogical Institute with foreign research centers. He directed five international conferences with the participation of eminent scientists. As the editor published 12 issues of “Bulletin of the International Centre for Asian Studies IGPU.” B. D. Pak has worked hard to establish scientific cooperation between scientific institutions of Russia and South Korea. He is the Member of the National Institute study of national history(Kuksa phyonch'an wiwonhve) and “outstanding researcher” of the Institute of the independence movement of Independence Memorial(Tonnip kinyomgwan). He was an active participant in international scientific conferences in the Republic of Korea, Japan, Hungary, USA. He was the scientific adviser of documentaries KBC(Yoksa Special). B. D. Pak actively cooperated and helped Korean organizations abroad, was honorary president of the Korean Association of Irkutsk. He led an active social and political work. Elected to the Council of the city of Irkutsk, he was a brilliant lecturer in international studies. Scientific and public activity of B. D. Pak was highly appreciated both at home and abroad. He was the first Russian Korean who was awarded the title “Honored Worker of Science of the Russian Federation”(1992), member of the Academy of Humanities(1995), was awarded the “Friendship” order, medals of the Russian Federation, Mongolia and the Republic of Korea. Interest in the works of B. D. Pak continues unabated even after his death. In 2011, Seoul “Russia and Korea”, translated into Korean, and in September “Soviet Koreans in the Great Patriotic War” were published. In October 2012 the book “An Junggeun - National Hero of Korea” was issued. B. D. Pak can be put on a par with people whose ideas laid the foundation of modern Korean studies. Many scholars of Russian-Korean relations, the Korean national liberation movement, the history of Russian Koreans call him their spiritual mentor. His great contribution to the development of Korean widely recognized.