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Ting-Ting Li,Shuang-Shuang Geng,Hui-Yan Xu,Ao-Lin Luo,Peng-Wei Zhao,Huan Yang,Xing-Wei Liang,Yang-Qing Lu,Xiao-Gan Yang,Ke-Huan Lu 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.1
Currently, the systems for culturing buffalo spermatogonial stem cells (SSCs) in vitro are varied, and their effects are still inconclusive. In this study, we compared the effects of culture systems with undefined (foetal bovine serum) and defined (KnockOut Serum Replacement) materials on the in vitro culture of buffalo SSC-like cells. Significantly more DDX4- and UCHL1-positive cells (cultured for 2 days at passage 2) were observed in the defined materials culture system than in the undefined materials system (p < 0.01), and these cells were maintained for a longer period than those in the culture system with undefined materials (10 days vs. 6 days). Furthermore, NANOS2 (p < 0.05), DDX4 (p < 0.01) and UCHL1 (p < 0.05) were expressed at significantly higher levels in the culture system with defined materials than in that with undefined materials. Induction with retinoic acid was used to verify that the cultured cells maintained SSC characteristics, revealing an SCP3+ subset in the cells cultured in the defined materials system. The expression levels of Stra8 (p < 0.05) and Rec8 (p < 0.01) were significantly increased, and the expression levels of ZBTB16 (p < 0.01) and DDX4 (p < 0.05) were significantly decreased. These findings provided a clearer research platform for exploring the mechanism of buffalo SSCs in vitro.
Some Physical Properties of a Surrounding-Gate MOSFET with Undoped Body
Guang-Xi Hu,Ting-Ao Tang 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.49 No.2I
A kind of Metal-Oxide-Semiconductor Field-Effect-Transistor (MOSFET) with a surrounding gate is presented. Some physical properties of the MOSFET are obtained by solving Poisson’s equation analytically. We propose a new criteria for the threshold voltage, and the threshold voltage is obtained in due course. The threshold voltage of a surrounding-gate nMOSFET is influenced by the thickness of the oxide and the radius of the cylinder. It’s also influenced by the work function difference between the gate electrode and intrinsic silicon. A MOSFET with a thinner oxide or a smaller radius will have a higher threshold voltage. We can obtain a desirable threshold voltage by choosing the right material for the gate and the right size for the MOSFET.
Guang-Xi Hu,Ran Liu,Ting-Ao Tang,Ling-Li Wang 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.6
Analytic solutions for the surrounding-gate (SG) metal-oxide-semiconductor field-effect transistor (MOSFET) are obtained by solving Poisson's equation. The analytic expressions for the electric potential and the threshold voltage are obtained by taking into account the short-channel effects. Our analytic results fit with other numerical and simulated results quite well. The analytic expressions will be of great help in ultralarge-scale integrated-circuit (ULSI) design. Analytic solutions for the surrounding-gate (SG) metal-oxide-semiconductor field-effect transistor (MOSFET) are obtained by solving Poisson's equation. The analytic expressions for the electric potential and the threshold voltage are obtained by taking into account the short-channel effects. Our analytic results fit with other numerical and simulated results quite well. The analytic expressions will be of great help in ultralarge-scale integrated-circuit (ULSI) design.
Pei-Ying Huang,Xin Yin,Yue-Ting Huang,Qi-Qing Ye,Si-Qing Chen,Xun-Jie Cao,Tian-Ao Xie,Xu-Guang Guo 연세대학교의과대학 2022 Yonsei medical journal Vol.63 No.5
Purpose: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the pathogen of coronavirus disease 2019. Diagnosticmethods based on the clustered regularly interspaced short palindromic repeats (CRISPR) have been developed to detect SARSCoV-2 rapidly. Therefore, a systematic review and meta-analysis were performed to assess the diagnostic accuracy of CRISPR fordetecting SARS-CoV-2 infection. Materials and Methods: Studies published before August 2021 were retrieved from four databases, using the keywords “SARS-CoV-2”and “CRISPR.” Data were collected from these publications, and the sensitivity, specificity, negative likelihood ratio (NLR), positivelikelihood ratio (PLR), and diagnostic odds ratio (DOR) were calculated. The summary receiver operating characteristic curve wasplotted for analysis with MetaDiSc 1.4. The Stata 15.0 software was used to draw Deeks’ funnel plots to evaluate publication bias. Results: We performed a pooled analysis of 38 independent studies shown in 30 publications. The reference standard was reversetranscription-quantitative PCR. The results indicated that the sensitivity of CRISPR-based methods for diagnosis was 0.94 (95% CI0.93–0.95), the specificity was 0.98 (95% CI 0.97–0.99), the PLR was 34.03 (95% CI 20.81–55.66), the NLR was 0.08 (95% CI 0.06–0.10), and the DOR was 575.74 (95% CI 382.36–866.95). The area under the curve was 0.9894. Conclusion: Studies indicate that a diagnostic method based on CRISPR has high sensitivity and specificity. Therefore, this wouldbe a potential diagnostic tool to improve the accuracy of SARS-CoV-2 detection.
A Resistive Memory in Semiconducting BiFeO<sub>3</sub> Thin‐Film Capacitors
Jiang, An Quan,Wang, Can,Jin, Kui Juan,Liu, Xiao Bing,Scott, James F.,Hwang, Cheol Seong,Tang, Ting Ao,Lu, Hui Bin,Yang, Guo Zhen WILEY‐VCH Verlag 2011 ADVANCED MATERIALS Vol.23 No.10
<P><B>A ferroelectric‐resistive random access memory consisting of a conductive BiFeO<SUB>3</SUB></B> epitaxial thin film with a unipolar diode current modulated by electric polarization orientation is reported. This device has a memory that lasts for months, a sufficiently high on current and on/off ratio to permit ordinary sense amplifiers to measure “1” or “0”, and is fully compatible with complementary metal‐oxide semiconductor processing. </P>
Characterization of proteolysis in muscle tissues of sea cucumber Stichopus japonicus
Chen-Chen Zhao,Yang Yang,Hai-tao Wu,Zhi-Mo Zhu,Yue Tang,Cui-Ping Yu,Na Sun,Qiang Lv,Jia-Run Han,Ao-Ting Li,Jia-Nan Yan,Yue Cha 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.6
The proteolysis in muscle tissues of sea cucumber Stichopus japonicus (sjMTs) was characterized. The proteins from sjMTs were primarily myosin heavy chains (MHCs), paramyosin (Pm), and actin (Ac) having a molecular mass of approximately 200, 98, and 42 kDa, respectively. Based on SDS-PAGE analysis and quantification of trichloroacetic acid (TCA)-soluble peptides released, degradation of muscle proteins from sjMTs was favorable at pH 5 and 50°C. Proteolysis of MHCs was mostly inhibited by cysteine protease inhibitors, including trans-epoxysuccinyl-L-leucyl-amido (4- guanidino) butane (E-64) and antipain (AP). E-64 and AP completely inhibited the degradation of Pm and Ac, while iodoacetic acid showed a partially inhibitory effect. These results indicated that the proteolysis of sjMTs was mainly attributed to cysteine proteases. Avoidance of setting the tissues at 40–50oC and slightly acidic condition and inhibition of cysteine proteases are helpful for decreasing sea cucumber autolysis.