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Xing-Wei Liang,Yong-Xun Jin,Ga-Young An,Seul-Ki Lee,Jung-Woo Kwon,Xiang-Shun Cui,Nam-Hyung Kim 한국동물번식학회 2012 Reproductive & Developmental Biology(Supplement) Vol.36 No.2s
It is well established that mitochondrial genome is strictly maternally inherited in mammalian, despite the fact that paternal mitochondria enter into oocyte during fertilization. To date, although some mechanisms have been extrapolated to interpret the elimination of paternal mitochondria, the exact mechanism still is unclear. Recent studies suggest that autophagy process and the ubiquitin-mediated degradation pathway may be involved in elimination of paternal mitochondria. However, the dynamic profiles of autophagy and ubiquitination associated with paternal mitochondria degradation have not been determined in mouse model. Through immunostaining with specific antibody LC3 and Ubiquitin and confocal microscopy, we investigated the dynamic profiles of LC3 and Ubiquitin signals in mouse embryos during preimplantation development. In addition, embryos were stained with MitoTracker Red for tracking the degradation process of paternal mitochondria. Our results showed that paternal mitochondria gradually degraded during postfertilization development, and sporadic paternal mitochondria were found at least in 16 cell embryos. LC3 and Ubiquitin signals appeared in the midpiece of sperm at 3 h postfertilization, and they were strictly colocalizated with paternal mitochondria from zygote to 2 cell embryo. Nevertheless, the colocalization became loose at 4 cell embryos, and gradually disappeared beyond 4 cell embryos. Our results confirmed that autophagy process and the ubiquitin-mediated degradation pathway may take part in the postfertilization remove of paternal mitochondria.
Xing-Wei Liang,Shao-Chen Sun,Yong-Xun Jin,Seul-Ki Lee,Jung-Woo Kwon,Xiang-Shun Cui,Nam-Hyung Kim 한국동물번식학회 2012 Reproductive & Developmental Biology(Supplement) Vol.36 No.2s
Superovulation, or ovarian stimulation is a commonly used ART for treatment of human infertility/subfertility. Recent studies suggest that superovulation unaffects methylated imprints acquisition in mouse oocytes during oogenesis, whereas disrupts DNA methylation maintenance in embryos during preimplantation development. However, the mechanisms of defects in methylation maintanence caused by superovulation remain largely unclear. We hypothesized that superovulation may disrupt the expression of DNA methyltransferases (Dnmts), the enzymes which catalyze DNA methylation acquisition and maintenance. The mice were subjected to superovulate with low (6 IU) and high (10 IU) dosage hormone. We examined the global DNA methylation levels in zygotes and DNA methylation of repeated sequences (IAP and Line 1) in blastocyst stage embryos. In addition, we investigated the expression of Dnmts (Dnmt3a, Dnmt3b, Dnmt3l and Dnmt1o) in ovulated oocytes and zygotes. Through staining with antibody 5mC and Di-H3K9 coupled with confocal microscopy, we found that global methylation profiles in zygotes derived from females after low or high dosage hormone treatment were not affected when compared to control counterpart. Moreover, methylation at IAP in blastocysts also was unaffected by superovulation, irrespective of hormone dosage. In contrast, methylation level at Line 1 decreased when the females were administered by high dosage hormone. Furthermore, expression of de novo DNA methyltransferase Dnmt3a, Dnmt3b, Dnmt3L, as well as maintenance Dnmt1o in MII oocytes and zygotes was not disrupted by superovulation. Given superovulation adversely affected methylation maintenance in blastocysts during preimplantation development but with normal expression of Dnmts in oocytes and zygotes, it is indicated that defects of embryonic methylation didn’t originate from abnormal expression of Dnmts.
성선자극호르몬의 투여가 생쥐 난자의 각인유전자의 DNA메틸화에 미치는 영향에 관한 연구
양흥휘(Xing-Wei Liang),김영훈(Yong-Xun Jin),이재달(Jae-Dal Lee),최향순(Xiang-Shun Cui) 충북대학교 동물생명과학연구소 2012 동물생명과학연구 Vol.4 No.-
성선자극호르몬에 의한 포유동물의 과배란 유도는 많이 사용되어 온 효과적인 방법이다. 그러나 이러한 과배란 유도방법에 의한 난포의 성장 및 성숙은 난자의 성숙과 함께 이루어지기에 난자의 각인유전자 발현과 동일한 시기로서 각인유전자의 DNA 메칠화에 영향을 미칠 염려도 있다. 본 연구의 목적은 과량의 성선자극 호르몬으로 난소를 자극하였을 때 난자 각인인자의 DNA 메칠화에 영향을 미치는지를 관찰 하는 것 이다. 대조군은 6주령된 ICR 암컷 생쥐에 48시간 간격으로 PMSG와 hCG를 각각 5 IU씩 복강투여 하였고 처리군은 같은 방법으로 PMSG와 hCG를 각각 10 IU씩 투여하였다. 그리고 성숙된 (MII단계) 난자는 hCG투여한 13-15시간 뒤에 난관으로부터 회수하여 각인인자 Snrpn 와 Peg3의 DNA 메틸화를 Bisulfite 시퀀싱 방법으로 분석하였다. 분석결과, PMSG를 5 IU 투여한 암컷 마우스의 성숙난자는 Snrpn 와 Peg3 유전자 모두 정상적인 메틸화 상태를 나타냈다. 그러나, 정량 보다 두 배의 PMSG를 투여한 생쥐의 난자는 Snrpn 유전자는 정상적인 메틸화 양상을 나타냈지만, Peg3 유전자는 대조군에 비해 비정상적인 DNA 메틸화 양상을 나타냈다. 이 연구결과는 과량의 성선자극호르몬의 투여는 난자 각인유전자의 DNA 메틸화에 영향을 미친다는 것을 시사한다. Superovulation or ovarian stimulation with gonadotropin is an effective way to enhance the availability of oocyte. Therefore, it is currently an indispensable ART (assisted reproductive technology) base on its significant benefit. However, ovarian stimulation was performed during oocyte growth, thus it may likely affect imprinted methylation as genomic imprinting was established in oocytes during the same time window. In current study, we investigated the impact of superovulation with excessive gonadotropin on methylated imprints in oocytes. MII oocytes were collected from female ssuperovulated with single-dose and double-dose PMSG. DNA methylation profiles of two imprinted genes Snrpn and Peg3 were evaluated by bisulfite sequencing. Our results showed that Snrpn and Peg3 were hypermetylated in the oocytes from single-dose superovulation females. In contrast, although all clones of Snrpn were hypermethyled in oocytes from double-dose superovulation females while some clones of Peg3 were hypomethylated. Our data demonstrated that genomic imprinting was correctly acquired in oocytes from females superovulated with single-dose gonadotropin while excessive stimulation affected genomic imprinting in oocytes, suggesting that DNA methylation in oocytes may be more susceptible to disruption of methylated imprint when superovulated with excessive gonadotropin.
Liang Bai,Huilin Wei,Bin Wang,Fangfang Liao,Tianhua Zhou,Xing-Wen Liang 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.47 No.1
This paper presented an investigation into steel tubes encased high-strength concrete (STHC) composite walls, wherein steel tubes were embedded at the boundary elements of high-strength concrete walls. A series of cyclic loading tests was conducted to evaluate the failure pattern, hysteresis characteristics, load-bearing capacity, deformability, and strain distribution of STHC composite walls. The test results demonstrated that the bearing capacity and ductility of the STHC composite walls improved with the embedding of steel tubes at the boundary elements. An analytical method was then established to predict the flexural bearing capacity of the STHC composite walls, and the calculated results agreed well with the experimental values, with errors of less than 10%. Finally, a finite element modeling (FEM) was developed via the OpenSees program to analyze the mechanical performance of the STHC composite wall. The FEM was validated through test results; additionally, the influences of the axial load ratio, steel tube strength, and shear-span ratio on the mechanical properties of STHC composite walls were comprehensively investigated.
Wei Liu, Di Huang,Zhao Liang Wu,Yanji Wang,Rui Li,Na Na Yin,Jian Xing Jiang 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.25 No.-
The purpose of this work was to separate isoflavone aglycones from soy whey wastewater after foam fractionation and acidic hydrolysis using chitosan microspheres. The maximal equilibrium adsorption capacity could be obtained at pH 6.0. The adsorption isotherm fitted better to the Freundlich equation model and the adsorption was an exothermic and spontaneous physical process. The maximal desorption ratio of isoflavone aglycones could reach 94.82% by using 70% (v/v) ethanol as desorption solution. Moreover, the regenerated chitosan microspheres could be reused for separating isoflavone aglycones from the feeding solution up to five times without compromising their function.
Liang-Yao Chen,Su-Xing Pan,Yun-Fei Wu,Lai Wei,Yu-Xiang Zheng,You-Yuan Zhao,De-Wang Ma 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.4
We have investigated the probing beam transmitance due to the photon-induced anisotropy effect in the bacteriorhodopsin/polymer composite film(bR/PC film) by using the probing-pumping beam method under different laser wavelengths. By using a theoretical simulation, we give the characteristics of the birefringence Δn and the dichroism ΔD for various wavelengths of the probing and the pumping beams.
Xing Li,Hong-Liang Lu,Hong-Ping Ma,Jian-Guo Yang,Jin-Xin Chen,Wei Huang,Qixin Guo,Jijun Feng,David Wei Zhang 한국물리학회 2019 Current Applied Physics Vol.19 No.2
Thin Ga2O3 films were grown on Si (100) using trimethylgallium (TMG) and oxygen as the precursors through plasma-enhanced atomic layer deposition. The depositions were made over a temperature range of 80–250 °C with a growth per cycle of around 0.07 nm/cycle. Surface self-saturating growth was obtained with TMG pulse time ≥20 ms at a temperature of 150 °C. The root mean square surface roughness of the obtained Ga2O3 films increased from 0.1 nm to 0.3 nm with increasing the growth temperature. Moreover, the x-ray photoelectron spectroscopy analysis indicated that the obtained film was Ga-rich with the chemical oxidation states Ga3+ and Ga1+, and no carbon contamination was detected in the films after Ar+ sputtering. The electron density of films measured by x-ray reflectivity varied with the growth temperature, increasing from 4.72 to 5.80 g/cm3 . The transmittance of Ga2O3 film deposited on a quartz substrate was obtained through ultraviolet visible (UV–Vis) spectroscopy. An obvious absorption in the deep UV region was demonstrated with a wide band gap of 4.6–4.8 eV. The spectroscopic ellipsometry analysis indicated that the average refractive index of the Ga2O3 film was 1.91 at 632.8 nm and increased with the growth temperature due to the dense structure of the films. Finally, the I-V and C-V characteristics proved that the Ga2O3 films prepared in this work had a low leakage current of 7.2 × 10−11 A/cm2 at 1.0 MV/cm and a high permittivity of 11.9, suitable to be gate dielectric.
Wei-Li Luo,Hui Wang,Yadong Li,Xing Liang,Tongyi Zheng 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.42 No.5
In this paper, a vibration-based method using the change ratios of modal data and the experience-based learning algorithm is presented for quantifying the position, size, and interface layer of delamination in laminated composites. Three types of objective functions are examined and compared, including the ones using frequency changes only, mode shape changes only, and their combination. A fine three-dimensional FE model with constraint equations is utilized to extract modal data. A series of numerical experiments is carried out on an eight-layer quasi-isotropic symmetric (0/-45/45/90)s composited beam for investigating the influence of the objective function, the number of modal data, the noise level, and the optimization algorithms. Numerical results confirm that the frequency-and-mode-shape-changes-based technique yields excellent results in all the three delamination variables of the composites and the addition of mode shape information greatly improves the accuracy of interface layer prediction. Moreover, the EBL outperforms the other three state-of-the-art optimization algorithms for vibration-based delamination detection of composites. A laboratory test on six CFRP beams validates the frequency-and-mode-shape-changesbased technique and confirms again its superiority for delamination detection of composites.