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- 저자 : Ke-Huan Lu (검색결과 2 건)
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USP18 promotes the growth in hemangiomas by regulating PI3K/AKT pathway
Ke Huan,Ma Xiang,Zeng Ying,Lu Jingjing,Fu Guili 대한독성 유전단백체 학회 2021 Molecular & cellular toxicology Vol.17 No.4
Background USP (ubiquitin-specific peptidase) 18 functions as deubiquitinating enzyme and participates in various human malignancies. The underlying mechanism involved in USP18-mediated hemangiomas progression has not been reported yet. Objective Immunohistochemistry analysis, qRT-PCR, and western blot were applied to detect USP18 expression in hemangioma tissues and cells. Lentiviral-mediated short hairpin RNA transfection was performed to silence USP18, and pcDNA-mediated USP18 over-expression was also performed. Cell Counting Kit-8 (CCK-8), colony formation assay and flow cytometry were applied to investigate cell viability, proliferation, and apoptosis, respectively. In vivo xenograft model was performed to detect function of USP18 on tumor growth. Results USP18 was enhanced in hemangioma tissues and cells compared to vascular endothelial tissues and vascular endothelial cells, respectively. Forced USP18 promoted cell viability and proliferation of human hemangioma endothelial cell (HemEC) and hemangioendothelioma endothelial cell (EOMA). Cell apoptosis of HemEC and EOMA were repressed by USP18 over-expression with reduced Bax and cleaved caspase-3. In contrast, silence of USP18 demonstrated the opposite effects on HemEC and EOMA cell viability, proliferation, and apoptosis. Silence of USP18 also retarded in vivo tumorigenicity of hemangiomas. Phosphorylation of AKT was enhanced by USP18 over-expression, while reduced by USP18 silence. Conclusion USP18 functioned as an oncogene in hemangiomas through acceleration of cell proliferation and repression of cell apoptosis, providing new insight for therapy of hemangiomas. Background USP (ubiquitin-specific peptidase) 18 functions as deubiquitinating enzyme and participates in various human malignancies. The underlying mechanism involved in USP18-mediated hemangiomas progression has not been reported yet. Objective Immunohistochemistry analysis, qRT-PCR, and western blot were applied to detect USP18 expression in hemangioma tissues and cells. Lentiviral-mediated short hairpin RNA transfection was performed to silence USP18, and pcDNA-mediated USP18 over-expression was also performed. Cell Counting Kit-8 (CCK-8), colony formation assay and flow cytometry were applied to investigate cell viability, proliferation, and apoptosis, respectively. In vivo xenograft model was performed to detect function of USP18 on tumor growth. Results USP18 was enhanced in hemangioma tissues and cells compared to vascular endothelial tissues and vascular endothelial cells, respectively. Forced USP18 promoted cell viability and proliferation of human hemangioma endothelial cell (HemEC) and hemangioendothelioma endothelial cell (EOMA). Cell apoptosis of HemEC and EOMA were repressed by USP18 over-expression with reduced Bax and cleaved caspase-3. In contrast, silence of USP18 demonstrated the opposite effects on HemEC and EOMA cell viability, proliferation, and apoptosis. Silence of USP18 also retarded in vivo tumorigenicity of hemangiomas. Phosphorylation of AKT was enhanced by USP18 over-expression, while reduced by USP18 silence. Conclusion USP18 functioned as an oncogene in hemangiomas through acceleration of cell proliferation and repression of cell apoptosis, providing new insight for therapy of hemangiomas.
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Ting-Ting Li,Shuang-Shuang Geng,Hui-Yan Xu,Ao-Lin Luo,Peng-Wei Zhao,Huan Yang,Xing-Wei Liang,Yang-Qing Lu,Xiao-Gan Yang,Ke-Huan Lu 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.1
Currently, the systems for culturing buffalo spermatogonial stem cells (SSCs) in vitro are varied, and their effects are still inconclusive. In this study, we compared the effects of culture systems with undefined (foetal bovine serum) and defined (KnockOut Serum Replacement) materials on the in vitro culture of buffalo SSC-like cells. Significantly more DDX4- and UCHL1-positive cells (cultured for 2 days at passage 2) were observed in the defined materials culture system than in the undefined materials system (p < 0.01), and these cells were maintained for a longer period than those in the culture system with undefined materials (10 days vs. 6 days). Furthermore, NANOS2 (p < 0.05), DDX4 (p < 0.01) and UCHL1 (p < 0.05) were expressed at significantly higher levels in the culture system with defined materials than in that with undefined materials. Induction with retinoic acid was used to verify that the cultured cells maintained SSC characteristics, revealing an SCP3+ subset in the cells cultured in the defined materials system. The expression levels of Stra8 (p < 0.05) and Rec8 (p < 0.01) were significantly increased, and the expression levels of ZBTB16 (p < 0.01) and DDX4 (p < 0.05) were significantly decreased. These findings provided a clearer research platform for exploring the mechanism of buffalo SSCs in vitro.
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