EFFECT OF DIETARY PROTEIN AND ENERGY LEVELS ON THE PERFORMANCES OF STARCROSS LAYERS

Salah Uddin, M.,Tareque, A.M.M.,Howlider, M.A.R.,Jasimuddin Khan, M.,Ahmed, S. Asian Australasian Association of Animal Productio 1992 Animal Bioscience Vol.5 No.4

Two similar experiments were designed to evaluate the interaction of dietary CP and ME levels on the production performance parameters at the age between 155 and 300 days in Starcross layers. In both experiments, the feed intake and mortality decreased but the egg weight, body weight gain and feed conversion efficiency increased as the dietary CP and ME levels increased. The CP intake was highest CP and lowest ME levels. With the increasing CP and decreasing ME levels, the ME intake decreased significantly (Experiments 1 & 2). The $CP{\times}ME$ interactions were significant only on mean egg weight and egg production in Experiment 2. In both the experiments, the CP levels were positively correlated with CP intake, egg weight, body weight gain and egg production and negative correlation with feed intake, mortality percentage and ME intake. The ME levels showed negative correlation with feed intake, protein intake, mortality percentages and positive correlation with all other parameters in both experiments. The highest values were noted for all the parameters (except mortality percentages) in Experiment 1 than that recorded in Experiment 2.

Acacia pennata L. leaves: chemical profiling and impact on DNA damage, alteration of genotoxicity—related genes expression and ROS generation in hepatic tissues of acetaminophen treated male rats

EL-Taher Eman M. M.,El-Sherei Moshera M.,El Dine Riham Salah,ElNaggar Dina M.Y.,Khalil Wagdy K. B.,Kassem Salwa M.,Elkhateeb Ahmed,Kassem Mona E. S. 경희대학교 융합한의과학연구소 2022 Oriental Pharmacy and Experimental Medicine Vol.22 No.1

From the phenolic profile of Acacia pennata L. leaves hydromethanolic extract, nine flavonoids were tentatively character- ized (liquid chromatography-electrospray ionization mass spectrometry), of which Kaempferol 3,7-di-O-hexoside, Apigenin 6,8-di-C-hexoside, Luteolin-6-C-pentoside-8-C-hexoside, Apigenin-6-C-hexoside-8-C-pentoside and Apigenin-6-C-pento- side-8-C-hexoside were detected for the first time from the plant. Three doses of the plant extract (12.5, 25 and 50 mg/kg bw/d, four weeks treatment) were used to assess its protective effect against acetominophen induced genotoxicity in hepatic cells of male rats (2 g/kg bw twice per week). A. pennata extract and Acetominophen treated animal groups attenuated DNA damage rates (Comet assay) by 5.6, 6.2 and 6.4% for the three doses, respectively, compared with the acetaminophen treated and untreated groups by 21.2% and 5.8%, respectively. It also decreased DNA fragmentation, significantly down-regulate the expression of p53 tumour suppressor gene and mdr1b multidrug resistance gene [real-time PCR (qPCR)] and ROS genera- tion rates induced by Acetaminophen by 715.4, 453.8 and 376.9% in rats in a dose dependant effect. The results highlighted the DNA protection potential of A. pennata phytoconstituents from drug abuse harmful effects.

Anti-proliferative Activities of Metallic Nanoparticles in an in Vitro Breast Cancer Model

Loutfy, Samah A,Al-Ansary, Nadia A,Abdel-Ghani, Nour T,Hamed, Ahmed R,Mohamed, Mona B,Craik, James D,Eldin, Taher A. Salah,Abdellah, Ahmed M,Hussein, Yassmein,Hasanin, MTM,Elbehairi, Serag Eldin I Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.14

Aims: To investigate effect of metallic nanoparticles, silver (AgNPs) and gold nanoparticles (AuNPs) as antitumor treatment in vitro against human breast cancer cells (MCF-7) and their associated mechanisms. This could provide new class of engineered nanoparticles with desired physicochemical properties and may present newer approaches for therapeutic modalities to breast cancer in women. Materials and Methods: A human breast cancer cell line (MCF-7) was used as a model of cells. Metallic nanoparticles were characterized using UV-visible spectra and transmission electron microscopy (TEM). Cytotoxic effects of metallic nanoparticles on MCF-7 cells were followed by colorimetric SRB cell viability assays, microscopy, and cellular uptake. Nature of cell death was further investigated by DNA analysis and flow cytometry. Results: Treatment of MCF-7 with different concentrations of 5-10nm diameter of AgNPs inhibited cell viability in a dose-dependent manner, with IC50 value of $6.28{\mu}M$, whereas treatment of MCF-7 with different concentrations of 13-15nm diameter of AuNPs inhibited cell viability in a dose-dependent manner, with IC50 value of $14.48{\mu}M$. Treatment of cells with a IC50 concentration of AgNPs generated progressive accumulation of cells in the S phase of the cell cycle and prevented entry into the M phase. The treatment of cells with IC50 concentrations of AuNPs similarly generated progressive accumulation of cells in sub-G1 and S phase, and inhibited the entrance of cells into the M phase of the cell cycle. DNA fragmentation, as demonstrated by electrophoresis, indicated induction of apoptosis. Conclusions: Our engineered silver nanoparticles effectively inhibit the proliferation of human breast carcinoma cell line MCF-7 in vitro at high concentration ($1000{\mu}M$) through apoptotic mechanisms, and may be a beneficial agent against human carcinoma but further detailed study is still needed.

Effect of chronic lead intoxication on the distribution and elimination of amoxicillin in goats

Ahmed M. Soliman,Ehab A. Abu-Basha,Salah A. H. Youssef,Aziza M. Amer,Patricia A. Murphy,Catherine C. Hauck,Ronette Gehring,Walter H. Hsu 대한수의학회 2013 Journal of Veterinary Science Vol.14 No.4

A study of amoxicillin pharmacokinetics was conducted in healthy goats and goats with chronic lead intoxication. The intoxicated goats had increased serum concentrations of liver enzymes (alanine aminotransferase and γ-glutamyl transferase),blood urea nitrogen, and reactivated δ-aminolevulinic acid dehydratase compared to the controls. Following intravenous amoxicillin (10 mg/kg bw) in control and lead-intoxicated goats,elimination half-lives were 4.14 and 1.26 h, respectively. The volumes of distribution based on the terminal phase were 1.19and 0.38 L/kg, respectively, and those at steady-state were 0.54and 0.18 L/kg, respectively. After intramuscular (IM)amoxicillin (10 mg/kg bw) in lead-intoxicated goats and control animals, the absorption, distribution, and elimination of the drug were more rapid in lead-intoxicated goats than the controls. Peak serum concentrations of 21.89 and 12.19 μg/mL were achieved at 1 h and 2 h, respectively, in lead-intoxicated and control goats. Amoxicillin bioavailability in the lead-intoxicated goats decreased 20% compared to the controls. After amoxicillin, more of the drug was excreted in the urine from lead-intoxicated goats than the controls. Our results suggested that lead intoxication in goats increases the rate of amoxicillin absorption after IM administration and distribution and elimination. Thus, lead intoxication may impair the therapeutic effectiveness of amoxicillin.

Synthesis, characterization, and in vitro antifungal activity of anionic and nonionic surfactants against crop pathogenic fungi

AHMED SALAH MAHMOUD TAWFIK,Mohamed F. Zaky,Tahany G.M. Mohammad,Hanaa A.E. Attia 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.29 No.-

Fungi have long been identified as causal agents of plant diseases. The development of plant antifungalactivity was attempted by synthesis of a new type of anionic and nonionic surfactant. The surfaceproperties were studied, including critical micelle concentration (CMC), effectiveness (pcmc), maximumsurface excess (Gmax), and minimum surface area (Amin). Free energy of micellization and adsorption wascalculated (DG8mic and DG8ads). The antifungal activity of the prepared surfactants was verified in vitro bythe poisoned food technique against eight phytopathogenic fungi: Colletotrichum dematium, Drechslerahawaiiensis, Fusarium oxysporum, Fusarium solani, Humicola fuscoatra, Pestalotia laurocerasi, Phoma spp.,and Phytophthra. The results reported that the percentage inhibition of mycelial growth increases withincreasing concentrations of the surfactant for all fungal species used. These results indicated that thesurfactant inhibited the mycelial growth of all fungal species in a dose-dependent manner. Blendsurfactants have a potential antifungal effect for the control of some phytopathogenic fungi.

Green gold@chitosan nanocomposite via solid-state synthesis; a separable catalyst for reduction of Cr(IV)

Ahmed Atia,Yasser K. Abdel-Monem,A.H. Salama,Salah M. El-kousy,Wael H. Eisa 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.117 No.-

A clean and high-throughput synthesis of gold@chitosan nanocomposite using nontoxic oxalic acid as areducing agent was developed. The synthetic route was carried out in completely dry conditions, i.e. solid-state reaction. The gold nanoparticles were bound with chitosan through the hydroxyl and aminogroups, as confirmed by Fourier transform infrared spectroscopic analysis. Scanning and transmissionelectron microscopy imaging confirmed the formation of semi-spherical gold particles with an averagesize of 8–15 nm. The catalytic performance of the synthesized gold nanoparticles for the reduction ofchromium(IV) in the presence of formic acid was evaluated. The film-forming ability of chitosan enabledthe design of separable/reusable heterogeneous catalyst which is convenient for real industrial applications. The proposed solid-state synthetic route could be adapted to produce a wide variety of nanostructuredmaterials that are characterized by high-yield, greenness, and low cost in very simple steps.

Percutaneous Transpedicular Fixation: Technical tips and Pitfalls of Sextant and Pathfinder Systems

Mohamed M. Mohi Eldin,Ahmed Salah Aldin Hassan 대한척추외과학회 2016 Asian Spine Journal Vol.10 No.1

Study Design: The efficacy of the operative techniques, possible benefits as well as pitfalls and limitations of the techniques are discussed. Potential drawbacks are also detected. Purpose: This study aims to report indications, techniques, and our experience with the use of the Sextant and PathFinder percutaneous transpedicular screw fixation systems. Overview of Literature: Percutaneous pedicle screw insertion is a novel technique. Successful percutaneous placement of pedicle screws requires surgical skill and experience because of lack of anatomic surface landmarks. Fluoroscopy-guided percutaneous placement of pedicle screws is effective. Many systems are now available. Methods: We conducted a prospective operative and postoperative analysis of 40 patients with absolute indication for thoracic or lumbar instability between January 2009 and June 2013. All procedures were performed with the Sextant (group A) and PathFinder (group B) systems under fluoroscopic guidance. Operative techniques are discussed and the results compared. Results: Percutaneous transpedicular screw fixation minimizes the morbidity associated with open techniques without compromising the quality of fixation. A total of 190 screws were inserted. There was no additional morbidity. Postoperative computed tomography images and plain X-rays were analyzed. Reduction of visual analog scale scores of back pain was evident. Conclusions: Fluoroscopy-guided percutaneous pedicular screws are feasible and can be safely done. Current systems allow multisegmental fixation with significantly less difficulties. The described techniques have acceptable intra- and postoperative complication rates, and overall sufficient pain control with early mobilization of patients.

Automatic Monitoring of Water Pollution from Sentinal-2 Satellite Imagery: A Case Study of Mediterranean Coast, Egypt

Magda Farhan,Mahmoud Salah,Ali M. Basha,Ahmed Kahlil,Omnia Fathy 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.8

Water is the most important source among the natural sources on the earth's surface for the health of marine and near-shore ecosystems. Assessing water pollution in coastal areas is an essential process for sustainable development. El Gharbia coast, Egypt is one of the most important coasts of Egypt. The main objective of this work is to predict the future changes of water pollution in this coast using Sentinel-2 satellite imagery of three consequent times. First, three Sentinel-2 satellite imagery of consequent dates were acquired and processed for further classification process. The maximum likelihood classification algorithm was then used to prepare the base maps for: time 1, time 2 and time 3, with ten major classes (pollutants). The classified images of time 1 and time 2 were then used to predict the time 3 water pollution map using a Markov Chain Model. After that, the final predicted water pollution map for time 3 was validated with the classified one of the same time. Finally, and compared with the water pollution map of time 3, the future ratios of all types of pollutants have been predicted. The results showed that the proposed model can simulate water pollution changes with reliable results. Based on the simulated water pollution map and by 2030, the ratios of all pollutants will increase. Accordingly, El Gharbia coast and surrounding activities can be saved from more pollution in the future.

Serum Biomarkers for Early Detection of Hepatocellular Carcinoma Associated with HCV Infection in Egyptian Patients

Zekri, Abdel-Rahman,Youssef, Amira Salah El-Din,Bakr, Yasser Mabrouk,Gabr, Reham Mohamed,El-Rouby, Mahmoud Nour El-Din,Hammad, Ibtisam,Ahmed, Entsar Abd El-Monaem,Marzouk, Hanan Abd El-Haleem,Nabil, M Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.3

Background: Early detection of hepatocellular carcinoma using serological markers with better sensitivity and specificity than alpha fetoprotein (AFP) is needed. Aims: The aim of this study was to evaluate the diagnostic value of serum sICAM-1, ${\beta}$-catenin, IL-8, proteasome and sTNFR-II in early detection of HCC. Materials and Methods: Serum levels of IL-8, sICAM-1, sTNFR-II, proteasome and ${\beta}$-catenin were measured by ELISA assay in 479 serum samples from 192 patients with HCC, 96 patients with liver cirrhosis (LC), 96 patients with chronic hepatitis C (CHC) and 95 healthy controls. Results: Serum levels of proteasome, sICAM-1, ${\beta}$-catenin and ${\alpha}FP$ were significantly elevated in HCC group compared to other groups (P-value<0.001), where serum level of IL-8 was significantly elevated in the LC and HCC groups compared to CHC and control groups (P-value <0.001), while no significant difference was noticed in patients with HCC and LC (P-value=0.09). Serum level of sTNFR-II was significantly elevated in patients with LC compared to HCC, CHC and control groups (P-value <0.001); also it was significantly higher in HCC compared to CHC and control groups (P-value <0.001). ROC curve analysis of the studied markers between HCC and other groups revealed that the serum level of proteasome had sensitivity of 75.9% and specificity of 73.4% at a cut-off value of $0.32{\mu}g/ml$ with AUC 0.803 sICAM-1 at cut off value of 778ng/ml, the sensitivity was 75.8% and the specificity was 71.8% with AUC 0.776. ${\beta}$-catenin had sensitivity and specificity of 70% and 68.6% respectively at a cut off value of 8.75ng/ml with an AUC of 0.729. sTNFR-II showed sensitivity of 86.3% and specificity of 51.8% at a cut off value of 6239.5pg/ml with an AUC of 0.722. IL-8 had sensitivity of 70.4% and specificity of 52.3% at a cut off value of 51.5pg/ml with AUC 0.631. Conclusions: Our data supported the role of proteasome, sICAM-1, sTNFR-II and ${\beta}$-catenin in early detection of HCC. Also, using this panel of serological markers in combination with ${\alpha}FP$ may offer improved diagnostic performance over ${\alpha}FP$ alone in the early detection of HCC.

Diagnosis of Clostridium difficile infection in patients with hospital-acquired diarrhea

Ibrahim Afifi, Salwa Selim,Gomaa, Fatma Alzahraa M.,Fathi, Lamia Fouad,Rasslan, Fatma Salah,Hamdy, Ahmed Mohamed The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.3

Clostridium difficile infection (CDI) is a rapidly emerging infection that may have devastating consequences. Prompt and accurate diagnosis is crucial for management and control. The aim of this study was to determine the incidence of C. difficile associated diarrhea among hospitalized patients, and to compare different diagnostic laboratory methods for detection of toxin producing strains in clinical specimens. The study was conducted at a university hospital in Cairo during the period from May 2013 till June 2015. Subjects were under antibiotic therapy and presented with hospital-acquired diarrhea. Four hundred and sixty-five stool specimens were processed by different microbiological methods. C. difficile was recovered in culture in 51 of stool specimens. Of these, 86.3% to 98% were positive for toxin production by 2 different methods. This study showed that antibiotic intake is the major risk factor for development of hospital-acquired diarrhea. We evaluated different microbiological methods for diagnosis of C. difficile. We recommend the use of toxigenic culture as a gold standard for microbiological diagnosis of C. difficile.